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2016 8 24 4 ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA August 2016 Vol. 24 No. 4 1, 1, 2, 2, (1., 201821;2., 200011) TGF β1, 240 SD, 185 148 5, TGF β1, 20, 1 21 42 d 63 d / ELISA TGF 1 TGF β1 21 d, /,TGF β1, ( P > 0 05) TGF β1, TGF β1; ; ; ; Q95 33 A 1005 4847(2016)04 0408 06 Doi:10 3969 / j. issn. 1005-4847 2016 04 014 Comparison of the therapeutic effect of TGF β1 gene therapy and traditional nonsurgical treatment on the rat model of postpartum stress urinary incontinence CHENG Ming jun 1, CAO Yun gui 1,DING Jing xin 2, HUANG Jian 2, (1 Shanghai Jiading Maternal and Child Health Hospital, Shanghai 201821,China; 2 Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011) Abstract Objective To study the effect of TGF β1 gene therapy on the rat model of postpartum stress urinary incontinence and explore a novel non operative treatment of this disease. Methods Two hundred and forty 6 month old SD female rats were used to prepare the model of postpartum stress urinary incontinence by vaginal dilation with a water sac. 148 rats from the 185 successfully prepared model rats were selected, and randomly divided into 5 groups: the TGF β1 gene therapy, clentuterol treatment, electric stimulation therapy, injection of empty vector plasmid, and non treated groups. In addition, 20 normal rats were selected as blank control group. Sneeze test and urodynamic test were conducted, the pelvic floor pubococcygeus muscle contractile force / muscle weight ratio was calculated, serum TGF 1 was detected by ELISA, and TGF 1 protein was detected by immunohistochemistry at 1, 21, 42 and 63 days after the treatment. Results At 21 days after treatment, all the maximum bladder capacity, leak point pressure, and urine or contractile force / muscle weight ratio of the TGF β1 gene therapy group showed even better effects than those of the electrical stimulation group, but the differences were statistically not significant ( P > 0 05). Conclusions TGF β1 gene therapy shows good therapeutic effect on the rat models of postpartum stress urinary incontinence, suggesting that TGF β1 gene therapy may become a new type of non surgical treatment for this disease. Key words TGF β1;gene therapy;stress urinary incontinence, postpartum; Rat Corresponding author: HUANG Jian. E mail: huangjian102@ sina. com [ ] ( :SHDC12013120); ( :ZK2015B08) [ ] (1976 - ),,,, Email: chengmingjun04@ 163. com [ ] (1978 - ),,, Email: huangjian102@ sina. com

2016 8 24 4 Acta Lab Anim Sci Sin,August 2016,Vol. 24 No. 4 409 (postpartum urinary inconti nence,ppui), (stress urinary incontinence, SUI), 10% ~ 60% [1-2],,,SUI,,,, PPUI β1 ( transforming growth factor β1,tgf β1),,,, [3],TGF β1,,,tgf β1, [4],, TGF β1, 1 1 1 1 1 1 :SPF,,6 Sprague Dawley (SD) 260, 310 ~ 350 g, [ SCXK ( ) 2012-0002] [SYXK( )2014-0029] 6,240,20 [5],, 8 Fr 2 cm, 8,, 300 g,8 h 1 1 2 :, (AD Instruments BIO Amp CF,QUAD Bridge), POWER LAB Chart v5 2 1, 10 ml / h,, ( ), (10 ml / h) ;, ;,,, 1 2 185 SD 128, 32 4 : (TGF β1), ( ),, 20,20 : 1 (TGF β1 ):, TGF β1 DNA 0 1 ml( 50 μg /, ); 0 5 cm 45 o 0 5 cm 2 ( ): ( : 1 5 V, 1 Hz, 0 1 ms, 2 s),,, 20, 3 3 ( ): 30 μg / ml, 3 4 ( ):, 5 ( ):, 0 1 ml /, 1 6 ( ): 1 21 42 d 63 d 1 4 8 5 6 5 1 3 ELISA TGF β1, 0 5 ml, - 80 ; ELISA TGF β1, RB ( Rapidbio),

410 2016 8 24 4 Acta Lab Anim Sci Sin,August 2016,Vol. 24 No. 4 DNM 9602 ; 1 4 TGF β1 : 10 min, 4%, SABC ( ) TGF β1 TGF β1 ( Abcam,ab92486) 1 100; 1 5, ;, ( JH 2), SKY A4, MFLab 3 01 ( ) 2 mm,, 3 6 V 1 Hz 20 ms,, 1 6 SPSS 13 0, Mean ± SD,, P < 0 05 2 2 1 240 SD PPUI,, 185, 77 1% ( 185 / 240), 0(0 / 20),, 1 d (P < 0 05 ), ( 1 2), 21 d TGF β1,,, ( P < 0 05 ), ; TGF β1, ( P > 0 05 ) Note. : : 21,P < 0 05 1 : Comparison between the clenbuterol, untreatment and null plasmid groups on D21, P < 0 05. Fig. 1 comparison of the maximum bladder capacity of the rats 2 2 TGF β1 TGF β1 ( 3),TGF β1, 1 d TGF β1 (P < 0 05),, TGF β1 TGF β1, (P < 0 05) 2 3 TGF βl, TGF βl,, ;,TGF βl 1 d TGF βl TGF βl, TGF β1 ( 4)

中国实验动物学报２０１６年８月第２４卷第４期注ＡｃｔａＬａｂＡｎｉｍＳｃｉＳｉｎＡｕｇｕｓｔ２０１６Ｖｏｌ２４Ｎｏ４与第２１天同次检测的药物治疗组未治疗组和空载质粒组比较Ｐ００５图２ＮｏｔｅＦｉｇ２注Ｃｏｍｐａｒｉｓｏｎｏｆｔｈｅｌｅａｋｐｏｉｎｔｐｒｅｓｓｕｒｅｏｆｔｈｅｒａｔｓ与第１天同次检测的药物治疗组电刺激组未治疗组和空载质粒组比较Ｐ００５图３Ｎｏｔｅ大鼠的漏尿点压力Ｃｏｍｐａｒｉｓｏｎｂｅｔｗｅｅｎｔｈｅｃｌｅｎｂｕｔｅｒｏｌｕｎｔｒｅａｔｍｅｎｔａｎｄｎｕｌｌｐｌａｓｍｉｄｇｒｏｕｐｓｏｎｄａｙ２１Ｐ００５血清ＴＧＦ β１含量Ｃｏｍｐａｒｉｓｏｎｂｅｔｗｅｅｎｔｈｅｃｌｅｎｂｕｔｅｒｏｌｐｈｙｓｉｃａｌｅｌｅｃｔｒｉｃｓｔｉｍｕｌａｔｉｏｎｕｎｔｒｅａｔｍｅｎｔａｎｄｎｕｌｌｐｌａｓｍｉｄｇｒｏｕｐｓｏｎｄａｙ２１Ｐ００５Ｆｉｇ３图４Ｆｉｇ４ＣｏｍｐａｒｉｓｏｎｏｆｔｈｅｓｅｒｕｍＴＧＦ β１ｌｅｖｅｌｓｏｆｔｈｅｇｒｏｕｐｓ注ＡＴＧＦ β１组Ｂ未治疗组Ｃ对照组耻尾肌组织ＴＧＦ β１表达均为治疗后１ｄ２００ＮｏｔｅＡＴＧＦ β１ＴＧＦ β１ｇｒｏｕｐＢＵｎｔｒｅａｔｍｅｎｔｇｒｏｕｐＣＣｏｎｔｒｏｌｇｒｏｕｐ２００ＥｘｐｒｅｓｓｉｏｎｏｆＴＧＦ β１ｉｎｔｈｅｐｕｂｏｃｏｃｃｙｇａｌｍｕｓｃｌｅｔｉｓｓｕｅａｔｏｎｅｄａｙａｆｔｅｒｔｒｅａｔｍｅｎｔ４１１

412 2016 8 24 4 Acta Lab Anim Sci Sin,August 2016,Vol. 24 No. 4 2 4 / / ( 5),, 1 d ( P > 0 05), 21 d TGF β1, (P < 0 05 ); TGF β1, (P > 0 05 ) Note. : 21,P < 0 05 5 : Comparison between the clenbuterol, untreatment and null plasmid groups on day21, P < 0 05. Fig. 5 Ratios of muscle contractile force / weight in the rat groups 3,, ( kegel ) [6,7],, [8],, [9],,,, [10] 2008 Carr PPUI, PPUI, [11-13],, ; SUI, [14,15] ( transforming growth factor,tgf), [3] ;,TGF β ECM, TGF βl [16] PPUI, ECM, PFD [17],TGF β1, [18], TGF β1 PFD TGF β1 PPUI,, TGF β1 PPUI, 1 d,, TGF β1,,,

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