760 203 26 6 J of Radioimmunology203 26 6 Adams RH Alitalo K. Molecular regulation of angiogenesis and lymphangiogenesis J. Nat Rev Mol Cell Biol 2007 8 464-478. 2 Ferrara N Gerber HP LeCouter J. The biology of VEGF and its receptors J. Nat Med 2003 9 669-676. 3 Hattori K Heissig B Wu Y et al. Placental growth factor reconstitutes hematopoiesis by recruiting VEGFR + stem cells from bonemarrow microenvironment J. Nat Med 2002 8 84-849. 4 Rakic JM Lambert V Devy L et al. Placental growth factor a member of the VEGF family contributes to the development of choroidal neovascularization J. Invest Ophthalmol Vis Sci 2003 44 386-393. VEGF 5 Fischer C Jonckx B Mazzone M et al. Anti-PLGF Inhibits growth of VEGF R -inhibitor-resistant tumors without affecting healthy vessels J. Cell 2007 3 463-475. 6 Witte L Hicklin DJ Zhu Z et al. Monoclonal antibodies targeting the VEGF receptor-2 Flk /KDR as an anti-angiogenic therapeutic tie-2 ephrinb2 Eph4 strategy J. Cancer Metastasis Rev 998 7 55-6. 7 Klement G Baruchel S Rak J et al. Continuous low-dose therapy with vinblastine and VEGF receptor-2 antibody induces sustained tumor IL-8 regression without overt toxicity J. J Clin Invest 2000 05 8 5-24. 8 Urake CJ Cheresh DA Little CD et al. An antagonist of integrin αvβ3 prevents maturation of blood vessels during embryonic neovascularization J. J Cell Sci 995 08 7 2655-266. 9 Burrows FJ Thorpe PE. Eradication of large solid tumors in mice with an immunotoxin directed against tumor vasculature J. Proc Natl Acad Sci USA 993 90 9 8996-9000. 0 Takahashi N Haba A Matsuno F et al. Antiangiogenic therapy of established tumors in human skin / severe combined immunodeficiency mouse chimeras by anti-endoglin CD05 monoclonal antibodies and synergy between anti-endoglin antibody and cyclophosphamide J. Cancer Res 200 6 2 7846-7854. Oh P Li Y Yu J et al. Subtractive proteomic mapping of the endothelial surface in lung and solid tumors for tissue-specific therapy J. Nature 2004 429 629-635. 203-08-5 203-08-9 DOI 0. 3969 /j. issn. 008-980. 203. 04. 039 IFN-γ 张腊红 洪理泉 罗 贤 2 葛胜祥 徐立群 * 陈兆军 γ- TB-IGRA 96 TB-IGRA 2 5 6 2 5 8 6 TB-IGRA 96 58 38 TB-IGRA 82. % 9. 7% 93. % 79. 3% P < 0. 0 TB-IGRA 2 6 2 5 8 P < 0. 0 TB-IGRA TST P < 0. 0 TB-IGRA TB-IGRA TB-IGRA TB-IGRA IFN-γ 2009A67 3005 2 36005 *
203 26 6 J of Radioimmunology203 26 6 76 Application of Mycobacterium Tuberculosis Specific Protein Interferon- Gamma Release Assay in Tuberculosis Diagnosis and Therapeutic Monitoring Zhang Lahong Hong Liquan Luo Xian et al Department of Clinical Laboratory The Affiliated Hospital of Hangzhou Normal University Hangzhou 3005 China Abstract Objective To investigate the applicative value of mycobacterium tuberculosis specific protein interferon gamma release test in early diagnosis of tuberculosis and the evaluation of clinical efficacy. Methods 96 clinical diagnosis of tuberculosis patients were related to the history collection and detected by TB-IGRA simueltaneously tuberculin skin test purified protein derivative of tuberculin 5 units tubercle bacillus culture directly smear tuberculosis antibody detection. Treatment of tuberculosis patients were followed up 2 5 6 months retreatment patients at 2 5 8 months in 6 months and years after the end of treatment followup once. Compared with difference between the IGRA and tuberculin skin test and other traditional laboratory results. Results Confirmed tuberculosis cases in 96 cases including 58 cases of pulmonary tuberculosis 38 cases of extra-pulmonary tuberculosis. The sensitivity of TB-IGRA in the pulmonary tuberculosis and extra-pulmonary tuberculosis showed 8% and 86. 8% respectively at the same time TB-IGRA in the diagnosis of tuberculosis the negative predictive value express 86. 3% specificity was 9. 7% and the positive predictive value was 88. 2%. In anti-tuberculosis treatment the positive rate of TB-IGRA and concentration levels before treatment and after treatment 2 5 6months retreatment 2 5 8months decreased significantly P < 0. 0. There is better correlation TB-IGRA concentration level with number of mycobacterium tuberculosis. It is significant difference compared to TB-IGRA with the traditional TST method to the evaluation of clinical treatment P < 0. 0. Conclusion The sensitivity of TB-IGRA in the diagnosis of tuberculosis and the postive predictive value is higher it can be used as a good basis for the diagnosis of tuberculosis. TB-IGRA provides a more reliable diagnosis method in patients with extra-pulmonary tuberculosis. Peripheral blood TB-IGRA decreased significantly in anti- tuberculosis treatment process. Coincidence rate between TB-IGRA concentration level and number of mycobacterium tuberculosis is higher. It is an ideal index to evaluate the clinical efficacy. Key Words interferon-gamma release test mycobacterium tuberculosis diagnosis evaluation of clinical treatment mycobacterium tuberculosis MTB /3 MTB 870 3% TB-IGRA HIV 40 00 HIV 2. 2009 7 ~ 200 2 272 90 MTB -6 ESAT-6-0 CFP-0 Th TB-IGRA T INH RFP PZA EMB 6 ~ 6 T T IFN-γ 0. 8 HIV interferon-gamma release assay IGRA 3 4 INH TB-IGRA > 4 2 3 4-6 TB-IGRA TB-IGRA 2 HIV TB-IGRA. 2 TB-IGRA PPD MTB TB-IGRA GNP-9270 ESCO
762 203 26 6 J of Radioimmunology203 26 6. 3 TB-IGRA BD 2. 3 TB-IGRA 58 4ml 2h 40 25. 3% ml N T P 3 37 68 43. 0% 38 22h ± 2h 3000r /min 0min 6 5. 7% 8 4-20 2. 0% TB-IGRA 8. 0% TB-IGRA 86. 8% P < 0. 0 TST. 4 TST P > 0. 05 PPD 5 TST 72h 43. % TB-IGRA 9. 7% TB-IGRA 93. % > 0mm. 5 TB-IGRA 2 3 4 ~ 8 2 TB-IGRA. 6 MTB n TB-IGRA TST IgG 58 28 68 40 25 0 38 33 8 6 30 23. 7 46 2 0 0 83 72 3 TB-IGRA. 8 2 6 2 5 8 79. % 78. 9% TB-IGRA 79. 3% TST % % % % 6 TB-IGRA TST TB-IGRA 82. 9. 7 93. 79. 3 TST 79. 43. 65. 60. 6. 9 SPSS. 5 63. 2 50. 6 63. 2 50. 6 χ 2 23. 5 00 00 49. 3 P < 0. 05 38. 8 00 00 54. 9 2 2. 4 TB-IGRA 2 2. 272 MTB 6 32 /96 67. 3% vs. 48 /96 24. 5% P < 0. 0 TST 96 2 6 47 /96 WHO 75. 0% vs. 9 /96 59. 2% P > 0. 05 58 38 8 TST 98 50. 0% 38 8 TB-IGRA 2 6. % 4 32 2 6 TB-IGRA 4 4. % 3. % PPD 47. 9% 5 7 4 2 46. 9% 4 2 6 4 TB-IGRA 4 2 2 2 46 TB-IGRA TB-IGRA TST TST n % n % 80 5 5 6 82. 55 79. 2. 2 TB-IGRA 58 2 32 67. 3 47 75. 0 28 TB-IGRA 38 5 83 42. 3 23 62. 75 33 TB-IGRA 46 34 6 48 24. 5 6 59. 2 TB-IGRA TB-IGRA 2 8. 0% 86. 8% TB-IGRA 6 82. % 9. 7% 93. % 8 32 20 2 6. 3 0. 2 6. 08 02 98 55. 52. 0 50. 0 79. 3% 6 8 4. 94 47. 9 6 3. 92 46. 9 TB-IGRA TB-IGRA 2. 5 2 TB-IGRA 28 33 2 50. 0% PPD 30 5 34 4. 3% 6 TB-IGRA 58 38 46 8. 6% PPD
203 26 6 J of Radioimmunology203 26 6 763 5 23 22 6 7 73 6 2 07 66 6 2 0 2 9 3 84 38 46 07 77 2 0 9 6 4 86 23 63 02 84 6 8 8 0 6 2 88 2 76 96 92 8 6 6 0 5 90 6 84 93 97 6 4 4 0 2 2 92 4 88 92 00 4 4 0 2 2 92 2 90 90 02 TB-IGRA P < 2. 6 TB-IGRA 0. 0 TST TB-IGRA 480 ± 24vs. 4 ± 2 P < 0. 0 8 P > 0. 05 43. % 6 TB-IGRA 9. 7% P < 0. 0 6 TB-IGRA TB-IGRA ng /ml 480 ± 24 2 20 ± 8 6 60 ± 5 2 30 ± 4 6 20 ± 3 8 4 ± 2 6 0 ± 0 2005 FDA QFT-G T-SPOT. TB TB-IGRA TB-IGRA TB-IGRA T TB-IGRA TB-IGRA MTB TB-IGRA TST TB-IGRA MTB MTB MTB T ESAT-6 CFP-0 γ- γ- MTB TST MTB PPD MTB 44. 4% IFN-γ 6 TB-IGRA TB-IGRA MTB T 95. 0% PPD Th IFNγ IFN-γ MTB 50. 0% 5 5 TB-IGRA 2 ELISA TB-IGRA n TST n n γ 46 45 4 5 ELISpot 50 6 34 4 36 γ T T T-SPOT. 2 38 36 2 35 3 TB TB-IGRA 58 96 62 2 46 MTB BCG 3 BCG T T MTB IFNγ ES- AT-6 CFP-l0 MTB TST PBMC IFN-γ PPD 2 MTB T Harari 3 MTB T TST TST T IL-2 BCG IFN-γ T IFN-γ 2 T MTB Lalvani 7 ELISPOT IFN-γ γ- MTB IGRA T
764 203 26 6 J of Radioimmunology203 26 6 TB-IGRA interferon gamma responses in children evaluated for tuberculosis J. 96 γ- Plos One 2009 4 e430. TB-IGRA 7 Lalvani A Pathan AA McShane H et al. Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T P < 0. 0 cells J. Am J Respir Crit Care Med 200 63 824-828. T IFN-γ 8 Ferrara G Losi M Meacci M et al. Routine hospital use of a new commercial whole blood interferon-gamma assay for the diagnosis of tuberculosis infection J. Am J Respir Crit Care Med 2005 72 63-635. T IFN-γ TB-IGRA MTB 9 Lee SW Lee CT Yim JJ. Serial interferon -gamma release assays TST P < 0. 05 during treatment of active tuberculosis in young adults J. BMC Infect TB-IGRA Dis 200 0 e300. World Health Organization. Global tuberculosis report 202 R. Geneva Switzerland World. 3 Andersen P Munk ME Pollock JM et al. Specific immune-based diagnosis of tuberculosis J. Lancet 2000 356 099-04. 4 Andersen P Doherty TM Pai M et al. The prognosis of latent tuberculosis can disease be predicted J. Trends in molecular medicine 2007 3 75-82. 5 Diel R Loddenkempr R Meywald-Walter K et al. Predietive value of a whole blood IFN-gamma assay for the development of active tuberculosis disease after recent infection with Mycobacterium tuberculosis J. Am J Respir Crit Care Med 2008 77 64-70. 6 Herrmann JL Belloy M Porcher R et al. Temporal dynamics of 0 Zhang S Shao L Mo L et al. Evaluation of gamma interferon release assays using Mycobacterium tuberculosis antigens for diagnosis of latent and active tuberculosis in Mycobacterium bovis BCG -vaccinated populations J. Clin Vaccine Immunol 200 7 2 985-990. 2 200. Mazurek GH Jereb J Lobue P et al. Guidelines for using the 202 34 8 485-508. Quanti FERON-TB Gold test for detecting Mycobacterium tuberculosis infection United States J. MMWR Recomm Rep 2005 54 49-55. 2 Dogra S Narang P Mendiratta DK et al. Comparison of a whole blood interferon-gamma assay with tuberculin skin testing for the detection of tuberculosis infection in hospitalized children in rural India J. Infect 2007 54 267-276. 3 Harari A Vallelian F Meylan PR et al. Functional heterogeneity of memory CD4 T cell responses in different conditions of antigen exposure and persistence J. Immunol 2005 74 037-045. 203-0-2 DOI 0. 3969 /j. issn. 008-980. 203. 06. 040 乔元彪 王 2 权 张文举 陈 * 沁 htsh htsh htsh 28 BSA BALB /c htsh 4E5 IgG κ McAb ELISA. 6 0 5 LH FSH HCG McAb htsh Preparation and Identification of Monoclonal Antibody Against h-thyroid-stimulating Hormone Qian Yuanbiao Wang Quan 2 Zhang Wenju Chen Qin * Shanghai University Shanghai 200444 2 Shanghai Veterinary Research Institute Shanghai 20024 China Abstract Objective To prepare high quality monoclonal antibody against human thyroid stimulating hormone h-tsh for highsensitivity detection. Methods After synthetic htsh β chain of 28 amino acids glutaraldehyde cross-linked method was adopted to * 200444 2 20024