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1 7 9 Vol. 7 No Journal of Food Safety and Quality Sep., 2016 杨娟 1 2,, 郭铮蕾 3 2,, 畅晓晖 3 2,, 杨向莹 3, 许美玲 1 2,, 尚士进 3, 饶红 2, 张锡全 3 2,, 陈广全 3 2, 3*, 张捷 2, 3, (1., ; 2., ; 3., ) 摘要 :,, 6, 关键词 : ; ; Application prospect of immunochromatographic technology for detection of food-borne pathogenic bacterium YANG Juan 1, GUO Zheng-Lei 2, 3, CHANG Xiao-Hui 2, 3, YANG Xiang-Ying 2, 3, XU Mei-Ling 1, SHANG Shi-Jin 2, 3, RAO Hong 2, 3, ZHANG Xi-Quan 2, 3, CHEN Guang-Quan 2, 3 2, 3*, ZHANG Jie (1. Linyi Entry-Exit Inspection and Quarantine Bureau, Linyi , China; 2. Inspection and Quarantine Technonical Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing , China; 3. Beijing Key Laboartory of Entry-Exit Food Safty Testing, Beijing , China) ABSTRACT: The immunochromatographic technology is one kind of immunoassay technique which based on chromotography techniques and the immune reaction between antigen and its antibody. In recent years, the frequent outbreak of foodborne illness at home and abroad has attracted more and more attention of the society. So a rapid and simple method for the detection of food-borne pathogenic bacterium is of great social significance in the prevention and diagnosis of foodborne illness. Immunochromatography has high sensitivity, strong specificity, easy operation and fast detection speed, which has been widely used in the detection of food. This paper reviewed the application of six kinds of immune markers on the detection of different kinds of pathogenic bacteria, and the characteristics of each method was been introduced. KEY WORDS: food-borne pathogenic bacterium; rapid detection; immunochromatographic technology 1 引言,,,,, * 通讯作者 :,, zhangjie@bjciq.goc.cn *Corresponding author: ZHANG Jie, Senior Engineer, Inspection and Quarantine Technonical Center of Beijing Entry-Exit Inspection and Quarantine Bureau; Beijing Key Laboartory of Entry-Exit Food Safty Testing, Beijing , China. zhangjie@bjciq.goc.cn

2 [1],, [2],,, ( PCR PCR PCR PCR ) (ELISA(enzyme linded immunosorbent assay) ) [3],, [4],,,, 2 免疫层析技术的检测原理 20 80, (nitrocellulose filter membrane, NC ),, NC ( ), NC ( ),, (5~10 min), [5], : [6] 2.1 双抗夹心免疫层析法的检测原理 - (antibody 1, Ab1), Ab1, (antibody 2, Ab2), T,,,,, -, (T ) - - T, T ( 1) 2.2 竞争免疫层析法的检测原理, :, T,,, T,, T, T, ;, T, T, Fig.1 1 Schematic diagram of immunochromatographic test strip

3 9, : 免疫层析技术在食源性病原菌检测中的应用,,, [7], [8] 3.1 胶体金免疫层析技术 ( : Au), (AuCl - 2 ), (H + ),,,,,,,, [9], 3(nonstructural protein 3, NS3) (core),, 96.5%, 97.4% [10] Ⅱ (PCV2), dcap (PCV2 dcap N ), PCV2 dcap, PCV (H 3 N 2 ) 479 ELISA 99.57%, 100%, 99.58% [11] PCR, nuc,,, cfu/ml, 0.5 cfu/ml,,, [12] (surface-enhanced Raman scattering, SERS),,, [13] B, 5~10 min, 8 ng/ml, 8~1000 ng/ml [14], E2 6E10, TCID 50,, [15] SERS,,,, SERS-,,,, [16], :, ; ; [17] 3.2 量子点免疫层析技术 (quantum dots, QDs), Ⅱ~ Ⅱ Ⅲ~ Ⅲ Si [18], 10 nm,, Tully [19] (Listeria monocytogenes), IntA IntB, cfu/ml [20] O157: H7, 5 min, cfu/ml, 8 [21] A(ochratoxin A, OTA), 0.05~1.0 ng/ml(y=0.259lnx+0.897, r 2 =0.995), OTA 0.05 ng/ml, 10 min 3.3 荧光微球免疫层析技术, ;, [22] NC,, 10~100 [23]

4 3506 7, 108 -, μg/ml [24] O139,, O139, cfu/ml [25] O157: H7, O157: H7, cfu/ml, cfu/ml, [26], 2.5 ng/ml [27],, A,, cfu/ml, cfu/ml, 15 min 3.4 上转磷光纳米免疫层析技术 (up-converting phosphor, UCP) UCP ( ) [28,29], UCP [30],, UCP,, UCP,, UCP,, [31,32],, [33] (Listeria monocytogenes, LM) (up-converting phosphor technology based lateral flow assay, UPT-LF), 15 min [34] O157, cfu/ml [35] ( ), 30 min,, 10 4 [36],, O157:H7 4 UPT-LF, 10 5 ~10 6 cfu/ml 3.5 纳米磁珠免疫层析技术 ( Fe 3 O 4 ) -,,,, (30 nm ), 3~10 [37],, O157 H7, [38],, HBsAg 0.1 ng/ml, ELISA, 5 min [39] M 1, M 1, 0.1 μg/l, M 1 (0.5 μg/l) 3.6 时间分辨荧光免疫层析技术 (time-resolved fluorescent immunochromatographic assay, TRFIA) 3, [40],, [41] TRFIA [42] SARS N TRFIA, 0.02~150 ng/ml, 0.02 ng/ml [43], B1, 0.05 ng/ml [44],

5 9, : 3507 B 1, 0.3 μg/kg 4 结语,,,,,, [45],,,, 参考文献 [1] Gould LH, Walsh KA, Vieira AR, et al. Surveillance for foodborne diseaseoutbreaks-united States, [J]. Mmwr Surveill Summ, 2013, 62(2): 1 34 [2],,,. [J]., 2005, 15(6): Chen GQ, Rao H, Pu PB, et al. Advances in viral hepatitis A and Norwalk-like virus detection methods in Food [J]. Inspect Quarant Sci, 2005, 15(6): [3],,,. [J]., 2014, 41(7): Li ZK, Su GC, Su WJ, et al. Progress of detection methods for norovirus [J]. Acta Microb Sin, 2014, 41(7): [4] Glod C, Grabb AO. Immunocapillary gration with enzymelabeled antibodies rapid quantification of c-reation protein in human plasma [J]. Anal Biochem, 1981, 116: [5],,,. [J]., 2007, 28(12): Wu G, Jiang ZM, Huo GC, et al. The application of colloidal gold immune chromatography technology in food detection [J]. Sci Technol Food Ind, 2007, 28(12): [6],,. [J]., 2011, 32(17): Li HM, Xu HY, Xiong YH. Research progress on the application of immunochromatographic test strip technology in foodborne pathogen detection [J]. Food Sci, 2011, 32(17): [7],,,. [J]., 2014, 35(8): Li JW, Song CM, Liu F, et al. Immunochromatographic Strip tecnology and its application in food safty detction [J]. Food Sci, 2014, 35(8): [8],,. [J]., 2011, 27(6): Liu DF, Deng XL, Lai WH. Application of novel chromatographic detection techniques for food safety [J]. Food Mech, 2011, 27(6): [9],, Tony Lee,. [J]., 2010, 24(8): Jiang SH, Yang FQ, Tony Lee, et al. Development and performance analysis of diagnostic reagents for hepatitis C [J], Guide China Med, 2010, 24(8): [10],,,. 2 [J]., 2014, 44(7): Song WJ, Hu BL, Jin YL, et al. Establishment of a method for detection of the antibody against porcine circovirus type 2 based on colloidal gold immunochromatographic assay [J]. Chin Vet Sci, 2014, 44(7): [11],,,. [J]., 2013, 4: Wang LL, Zhao Y, Yu CN, et al. Research on nucleic acid lateral flow method for rapid detection of Staphylococcus aureus in food [J]. Food Eng, 2013, 4: [12],,,. [J]., 2009, 4: Chen X, Feng L, Liu S, et al. The Design of quantitative testing equipment for chromatographic colloidal-gold strips [J], J Heibei Univ Technol, 2009, 4: [13],,,. B[J]., 2010, 22(1): Xie SJ, Wang J, Jiang YQ, et al. Rapid and quantitative detection of staphyloccal enterotoxin B with colloid Gold immunochromatography [J]. Chin J Food Hyg, 2010, 22(1): [14],,,. [J]., 2010, 32(6): Wang XP, Sun Y, Yang ZQ, et al. Development of a colloidal gold immunochromatographic assay for antigen detection of wild-type classical swine fever virus [J]. Chin J Prev Vet Med, 2010, 32(6): [15]. (SERS-ICA) [D]. :, Chang HF. Studies and applications of surface-enhanced Raman spectroscopy based immunochromatographic assay (SERS-ICA) [D]. Suzhou: Soochow University, [16],. [J]., 2014, 17: Zhang JJ, Cui XC. Application of colloidal gold technique in immune chromatography [J]. Jiangsu Sci Technol Inf, 2014, 17: [17],,. [J].,2004, 6: 1 7. Mou Y, Wang N, Jin QH. Application and development of quantum dots to life science [J]. Micronanoelectr Technol, 2004, 6: 1 7. [18],,,. [J]., 2014, 5(7): Tang YW, Gao ZY, Wei LQ, et al. Progress on immunolabelling chromatographic techniques in food safety detection [J]. J Food Saf Qual, 2014, 5(7):

6 [19] Tully E, Hearty S, leonard P, et al. The development of rapid fluorescence-based immunlassays,using quantum dot-labelled antibodies for the detection of listeria monocytogences cell surface proteins [J]. Int J Biol Macromol, 2006, 39(1 3): [20],,,. O157:H7 [J]., 2015, 4: Yuan LJ, Li ML, Wang SY, et al. Study on quantum dot immune chromatography test strip for Escherchia coil O157:H7 [J]. Food Mech, 2015, 4: [21],,,. A[J]., 2015, 43(12): Zhou YF, Xiong SC, Jiang H, et al. Immunochromatographic assay for quantitative detection of ochratoxin A in maize by quantum dot beads [J]. Chin J Anal Chem, 2015, 43(12): [22],. [J]., 2015, 25(22): LY, Wang YL. Research progress of immune chromatography [J]. Chin J Health Lab Technol, 2015, 25(22): [23],,,. [J]., 2015, 15(2) Li JT, Hou SP, Ji ZW, et al. The establishment of immunofluorescence chromatographic detection method of Toxins in Seashell [J]. J Trop Med, 2015, 15(2): [24],,,. O139 [J]., 2010, 2(1): Fan F, Zhu H, Hong XL, et al. Rapid detection of Vibrio cholera O139 with fluorescent nanoparticles based lateral flow strip assay [J]. J Mol Diag Ther, 2010, 2(1): [25],,,. O157: H7 [J]., 2013, 34(16): Xie QY, Lai WH, Liu CM, et al. Development of fluorescent microsphere immunochromatographic strip for detection of Escherichia coli O157:H7 [J]. Food Sci, 2013, 34(16): [26],,,. [J]., 2012, 28(1): Liu DF, Deng XL, Lai WH, et al. Development of lateral flow assay of ractopamine based on fluorescent microspheres [J]. Food Mech, 2012, 28(1): [27],,,. [J]., 2015, 38(3): Shi YM,He H, Tan H, et al. Establishment of a fluorescent nanoparticle labeled immunochromatographic assay for the rapid detection of Staphylococcus aureus [J]. Chin J Front Health Quarant, 2015, 38(3): [28] Hong W, Huang L, Wang H, et al. Derelopment of up-converting phosphor teachnology-based 10-channel lateral flow assay for profiling antibodies yersinia [J]. J Microbiol Meth, 2010, 83(2): [29],,,. AFP [J]., 2009, 19(15): Zhao LJ, Zhou L, Fang F, et al. Development of up-converting phosphor immunochromatography test for quantitative detection of AFP [J]. Prog Mod Biomed, 2009, 19(15): [30]. HBsAg [J]., 2012, 14(2): Lei WQ. Evaluation of quantitative Immunochromatography assay use in blood screen for HbsAg [J]. J Clin Transfus Lab Med, 2012, 14(2): [31] Qu Q, Zhu Z, Wang Y, et al. Rapid and quantitative detection of Brucella by up-converting phosphor technology based lateral-flow assay [J]. J Microbiol Meth, 2009, 79(1): [32] Li L, Zhou L, Yu Y, et al. Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody[j]. Diagn Microbiol Infect Dis, 2009, 63(2): [33],,,. [J]., 2014, 38(9): Liu X, Li CF, Wang XY, et al. An up-converting phosphor technology based lateral flow assay for rapid detection of Listeria monocytogenes [J]. Mil Med Sci, 2014, 38(9): [34],,,. O157:H7[J]., 2007, 19(1): Wang J, Zhou L, Li W, et al. Development of UPC-immunochromatography test for rapid detection of O157:H7 [J]. Chin J Food Hyg, 2007, 19(1): [35],,,. [J]., 2006, 26(8): Li W, Zhou L, Wang J, et al. Development of up-converting phosphor immunochromatography for fast and quantitive detection of Bad lusanthracisspore [J]. Chin J Microbiol Immunol, 2006, 26(8): [36],,,. [J]., 2015, 39(2): Li CF, Zhao Y, Wang XY, et al. Development of up-converting phosphor technology based lateral flow assay for quantitative detection of foodborne pathogens [J]. Mil Med Sci, 2015, 39(2): [37],,,. O157:H7[J]., 2013, 41(12): Cui X, Xiong QR, Xiong YH, et al. Establishment of a method combined immunomagnetic separation with colloidal gold lateral flow assay and its application in rapid detection of Escherichia coli O157 H7 [J]. Chin J Anal Chem, 2013, 41(12): [38],,,. [J]., 2012, 23(1): Sun JB, Wang WH, Lei XY, et al. Development of a rapid quantitative magnetic nanoparticle-based immunochromatographic test for human hepatitis B surface antigen [J]. Lett Biotech, 2012, 23(1): [39],,,. M1 [J]., 2014, 42(5): Huang YM, Liu DF, Lai WH, et al. Rapid detection of aflatoxin M1 by immunochromatography combined with enrichment based on immunomagnetic nanobeads [J]. Chin J Anal Chem, 2014, 42(5): [40] Hu Z, Li M, Huang B, et al. Detection of hepatitis B virus PreS1 antigen using a time-resolved fluoroimmuno assay [J]. J Immunoass Immunoch, 2012, 33(2): [41],,,. [J]., 2006, 27(12):

7 9, : 3509 Jin J, Lai WH, Tu ZX, et al. Research progress and applications in food safety of time-resolved fluoroimmunoassay [J]. Food Sci, 2006, 27(12): , 2014,41(7): Li ZK, Su GC, Su WJ, et al. Progress of detection methods for norovirus [J]. Acta Microb Sin, 2014, 41(7): [42],,,. SARS [J]., 2005, 25(4): ( 责任编辑 : 刘丹 ) Wang L, Wu YS, Tang YP, et al. Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay [J]. J First Mil 作者简介 Med Univ, 2005, 25(4): [43],,,. B1 [J]., 2009, 26(12): Zhang Y, Zhu S, Chen J, et al. Establish the ultra trace detection method to 杨娟, 本科, 工程师, 主要研究方向为食品微生物检测 yangjuan0426@163.com distinguish fumonisin B1 time-resolved fluoroimmunoassay [J]. J Environ Health, 2009, 26(12): [44],,,. [J]., 2014, 47(18): Zhang ZW, Li PW, Zhang Q, et al. Study on time-resolved fluorescence immunochromatography for afaltoxin determination in agricultural product [J]. Sci Agric Sin, 2014, 47(18): 张捷, 博士, 高级工程师, 主要研究方向为食品微生物检测及食品安全检测评估 zhangjie@bjciq.goc.cn [45],,,. [J].

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