Microsoft Word _排版二校出376

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馗臻龛武
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1 8 2 Vol. 8 No Journal of Food Safety and Quality Feb., 2017 * 谢瑜杰, 呼秀智, 孙晓铮, 薛占永 (, ) 摘要 :,,, -,,, 关键词 : ; ; Research progress of application of colloidal gold assay in determination of sulfonamides residues in aquatic products XIE Yu-Jie, HU Xiu-Zhi, SUN Xiao-Zheng, XUE Zhan-Yong * (Hebei University of Engineer, Handan , China) ABSTRACT: In recent years, due to the appearance and extensive use of antibiotics especially the wide usage of broad-spectrum and cheap sulfonamides in aquaculture for the prevention and treatment of bacterial diseases, it caused sulfonamides residues in aquatic products. This paper introduced sulfonamides and their harm, and briefly reviewed the application of colloidal gold immunochromatographic assay in the detection of sulfonamides in aquatic products. Compared with enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC-MS/MS) detection method and so on, colloidal gold immunochromatography technology is inexpensive, easy to use, fast and accurate, but its application is not so extensive, so how to improve the sensitivity of colloidal gold should be studied in the future, so that it can be able to detect a variety of drug residues in aquatic products. KEY WORDS: colloidal gold assay; sulfonamides residues; aquatic products 1 引言,,,, [1] [2], [1],, [3] * 通讯作者 :,,, @qq.com *Corresponding author: XUE Zhan-Yong, Associate Professor, Master Tutor, Hebei University of Engineer, Handan , China @qq.com

2 376 8,,, [4],, [5-7],,,, [8], 100 μg/kg, 20 μg/kg [5, 9-11] , 0.1 mg/kg [7,11-13], [5] (enzyme linked immunosorbent assay, ELISA) [8] (high performance liquid chromatography, HPLC) [5] - (liquid chromatography- tandem mass spectrometry, LC-MS/MS) [10] [5] 2 磺胺类药物介绍及其危害 2.1 磺胺类药物的作用原理 [11], [12],, [13],,,,, [7],,,, [11] 2.2 磺胺类药物残留的危害 [14], [5] [15],,, [5], [5] ;, [7] ;, 3, [16,17], [7] ;,, [6] ;, Wang [9], 3 磺胺类药物残留检测的研究现状,,, [18] 3.1 常用检测方法 1993, 100 ng/kg, [7] 20, [19-21],, [13],,, [7] - [7,22],,, ;, [23] (enzymelinked immunosorbent assay, ELISA) [13] [24], ELISA, 3.2 胶体金免疫层析技术, [25] 20 80, Faulk Taylor [26-28] 3, [29] ELISA,,,, (10~15 min),,,,,, Geoghegan Danscher Faulk Taylor [28],, [30],,

3 2, : 377 [28] [5] [6], 2, (T) (C) [31], T C, T C,, ( 100 ng/kg) [6] ; T C,, ; T,, T T C, [6], [32],, T C, ;, [33] 噁, 50 ng/ml, 5~10 min [34] SD 10 ng/ml, 500 ng/ml,, 100% [35],, 20 ng/ml [36] [11], [28],, 8, [37] [5], [30], [38],,, [39-43] 4 展望, [44-47],, [48-50],, [30],, 参考文献 [1],,,. [J]., 2016, 7(5): Gao LX, Huang ZK, Feng M, et al. Research progress of rapid detection in aquatic product safety testing [J]. J Food Saf Qual Test, 2016, 7(5): [2],,. [J]., 2013, 12: Zhang XY, Liu ZQ, Li Q. Application of colloidal gold immunochromatographic strip in aquaculture [J]. Biotechnol Bull, 2013, 12: [3],,,. [J]., 2005, (5): Li YD, Zhang SE, Wu ZG, et al. Rapid detection of chloramphenicol residue by colloidal gold immunochromatographic assay [J]. Chin J Food Hyg, 2005, (5): [4]. [D]. :, Cao YY. Study on the detection of veterinary drug residues in animal food and the market investigation of veterinary drug residues in animal products sold in Jinan [D]. Jinan: Shandong University, [5]. [D]. :, Luo M. Application of colloidal gold immunochromatographic strip for detection of sulfonamides residues in aquatic products [D]. Wuhan: Huazhong Agricultural University, [6]. ELISA [D]. :, Du YL. A preliminary study on the rapid detection kit for sulfonamides multi residues ELISA and colloidal gold immunochromatographic strip [D]. Urumchi: Xinjiang Agricultural University, [7]. [D]. :, Chen JZ. Study on the detection of sulfonamides residues [D]. Shanghai: Shanghai Jiao Tong University, [8],,,. [J]., 2004, 14(1): Guan R, Gu M, Wei WG, et al. Establishment of an ELISA for the detection of sulfadiazine residues in animal tissues [J]. Inspect Quarant Sci, 2004, 14(1): [9] Wang L, Wang S, Zhang J, et al. Enzyme-linked immunosorbent assay and colloidal gold immunoassay for sulphamethazine residues in edible animal foods: investigation of the effects of the analytical conditions and the sample matrix on assay performance [J]. Anal Bioanal Chem, 2008, 390(6): [10] Gao J, Cui Y, Tao Y, et al. Multi-class method for the quantification 92 veterinary anti microbial drugs in livestock excreta, wastewater, and surface water by liquid chromatography with tandem mass spectrometry [J]. J Sep Sci, 2016, 39(21): [11]. [D]. :, Han J. Study on colloidal gold immunochromatographic assay of sulfonamides [D]. Tianjin: Tianjin University of Science and Technology, [12]. [D]. :, 2009.

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Microsoft Word _排版 二校 出376

Microsoft Word _排版二校出376