Microsoft Word 欧阳凡-二校出4378.doc

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1 6 11 Vol. 6 No Journal of Food Safety and Quality Nov., , 欧阳凡 2*, 张遴 1, 董文宾 2, 王昌钊 (1., ; 2., ) 1 摘要 :,,,,, 关键词 : ; ; Research progress on the rapid propagation of Dendrobium officinale OUYANG Fan 1, 2, ZHANG Lin 1*, DONG Wen-Bin 2, WANG Chang-Zhao 1 (1. Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi an , China; 2. College of Food & Biological Engineering, Shaanxi University of Science & Technology, Xi an , China) ABSTRACT: Dendrobium officinale has high economic and healthy value, but its shortage of wild resources constrained the increasing market demand. Nowadays, current researches in tissue culture and artificial propagation techniques have been made a certain progress. In this paper, the researches of tissue culture and rapid propagation of D.officinale were reviewed from the aspects of selection of explants, hormone and natural additives. Recent research achievements were summarized and the future research directions were discussed in order to provide theoretical basis for achieving D.officinale magnifying production. KEY WORDS: Dendrobium officinale; tissue culture; rapid propagation 1 引言 (Dendrobium officinale Kimura et Migo),,, 114,,,,, [1],,, [2],,, 1987,,, [3],,,, * 通讯作者 :,, oouuyyff@163.com *Corresponding author: OUYANG Fan, Postgraduate, Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi an China. oouuyyff@163.com

Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi an 710068, China; 2.

2 11, : 选取材料和培养条件 2.1 外植体的选取 : [4-6] [7-15] [16-24] [25,26] [27],,,,, [28],,, [29], [8,18], 2.2 培养条件与组培过程控制 ph [30] 12 h/d, 68 μmol m 2 s 1,, 25 [24],,,,, [20] ph ph 5.8, ph 5.8 [31] 3, [18], [17], [32] 14 min, 100%,, 6~8 min [14], 50% [15] 70% 30 s, 0.1% 9 min,,, [13] 6, [32], C [10] 3 不同生长阶段的因素控制 3.1 种子萌发,,, MS 1/2MS KC B5 N6, [4] 3, 1/2MS, MS,, [5] MS,, MS, [2], MS, NAA, mg/l [5] ; 6-BA, [2] 1 mg/l KT IAA [33], ABA, [6] ABA [34], [5], 3.2 茎段培养,,,,,,,, [35] MS 1/2MS [9] MS 1/2MS 100%, MS, MS, [15] [36], [13,19] 1/2MS,, /,

8 [31] 3, [18], [17], [32] 14 min, 100%,, 6~8 min [14], 50% [15] 70% 30 s, 0.1% 9 min,,, [13] 6, [32], C [10] 3 不同生长阶段的因素控制 3.

3 [11] [13] 3 KT ZT 6-BA, 6-BA, 2 mg/l,,, mg/l NAA 6-BA, NAA 6-BA, [8],,, 15 / [9] 3.3 原球茎增殖 MS, Nitch, Knudson C(KC), N6, B5, C17, SH, 1/2 MS, MS [18] 5, MS, 1/2 MS MS, B5 MS KC N6 [2], 1/2MS, [19],, [4,5],,,, NAA KT 6-BA IAA IBA GA [5] NAA KT 6-BA,, [16], 6-BA 2.0~3.0 mg/l NAA 0.2~0.5 mg/l,,, 2,,, ;, [20],, 100 mg/l, NAA,, [37] [5],, 3.4 原球茎分化, MS 1/2MS, NAA 6-BA, NAA, 6-BA ; 6-BA NAA,, NAA [2],, [2] 3.5 生根与壮苗,,,, MS 1/2MS [36] MS 1/2MS KC 1/2KC, MS KC NAA 6-BA IBA IAA 6-BA, NAA, / [37] NAA 6-BA [5], 6-BA, NAA, NAA, 6-BA [2] NAA IBA, [8], NAA IBA IAA [30],, [16,29] [30] 3.6 出瓶移栽,, [38],,, 2 3 cm [26] [39] +10% 50% +50% 0 2 cm

6-BA,, [16], 6-BA 2.0~3.0 mg/l NAA 0.2~0.5 mg/l,,, 2,,, ;, [20],, 100 mg/l, NAA,, [37] [5],, 3.

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A preliminary study of Dendrobium soilless growing media [J]. China J Chin Mater Med, 2000, 25(1): 23 24. [42],,. [J]., 2014, 7(5): 2131 2134. Li HJ, Jiang Y, Lin CH.

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<D2BDC1C6BDA1BFB5CDB6C8DAD7CAB8DFB7E5C2DBCCB3B2CEBBE1C3FBB5A52E786C7378> 参会人员名单 Last Name 姓名公司 Tel Fax Bai 柏煜康复之家 8610 8761 4189 8610 8761 4189 Bai 白威久禧道和股权投资管理 ( 天津 ) 有限公司 8610 6506 7108 8610 6506 7108 Bao 包景明通用技术集团投资管理有限公司 8610

Microsoft Word 欧阳凡-二校 出4378.doc

Microsoft Word 欧阳凡-二校出4378.doc