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1 7 5 Vol. 7 No Journal of Food Safety and Quality May, 2016 任舒悦 1 1,, 姜会聪 2, 彭媛 1, 白家磊 1, 宁保安 1, 高志贤 (1.,, ; 2., ) 1* 摘要 :,,,,,,,,, 关键词 : ; ; Up-converting phosphor technology-based immunochromatographic assay and its application in the field of food safety detection REN Shu-Yue 1, JIANG Hui-Cong 1, 2, Peng Yuan 1, BAI Jia-Lei 1, NING Bao-An 1, GAO Zhi-Xian 1* (1. Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Health and Environment Medicine, Military Medical Sciences, Tianjin , China; 2. School of Public Health, Zhengzhou University, Zhengzhou , China) ABSTRACT: Food safety is one of important things in the healthy lives of billions of people, and rapid detection technology for food safety was constantly progressive. The traditional physical and chemical methods exist many problems to be solved, such as complex sample preparation, time consumption and high cost, which can only be used for confirming the suspected samples but not for the screening on the scene. However, up-converting phosphor (UCP) technology-based immunochromatographic assay, a new technique, which is based on functional up-converting phosphor and the special combination of antigen-antibody, has the characteristics of high sensitivity, high specificity, ease to use, rapid detection and so on, being widely used in the process of rapid detection. In this paper, it took a brief introduction to immunochromatographic assay and UCP technology. Mainly, it also reviewed the progress of the application of this technology and its application in food safety detection. Through the analysis of the advantages and 基金项目 : ( ) (14ZCZDSF0021) Fund: Supported by the National Natural Science Foundation of China ( ) and the Science and Technology Support Projects of Tianjin (14ZCZDSF0021) * 通讯作者 :,, gaozhx@163.com *Corresponding author: GAO Zhi-Xian, Researcher, Institute of Health and Environment Medicine, No.1, Dali Road, Tianjin , China. gaozhx@163.com

2 5, : 1859 disadvantages of the present technology, the development direction was put forward according to its application field at present, which provided a reference for the further application of the technology in the field of food safety. KEY WORDS: up-converting phosphor; immunochromatographic assay; food safety detection 1 引言,,,,,,,,,, [1], DNA (ELISA), [2-4], [5], 2 上转换发光免疫层析技术 2.1 免疫层析技术 (immunochromatographic assay, ICA) -,,,,,,, [6],,, ; 焠, 2.2 上转换发光技术 (up-converting phosphor, UCP), f UCP, [7], UCP, (up-converting phosphor technology, UPT) UPT, [8-12] [13-18],, [19] 2.3 上转换发光免疫层析技术 (up-converting phosphor technology-based immunochromatographic assay), 5 :,,, UCP,,, UCP C T,, UPT ( 1), (T) (C) UCP, T/C, [20] UCP,,,, [21],, [22] 3,

1860 7, ( ), ( O157) UPT UPT [23-25],, [28] 10 UPT UPT-LF ( 2) O1 O139 10 4 CFU/mL; O157 10 5

2 Ten channel test strip structure diagram [28] (left for the internal structure, right for

1 Immunochromatography reaction process and the test card [20] 3 上转换发光免疫层析技术在食品安全检测中的应用 3.

3 1860 7, ( ), ( O157) UPT UPT [23-25],, [28] 10 UPT UPT-LF ( 2) O1 O CFU/mL; O CFU/mL; 10 6 CFU/mL; 10 7 CFU/mL 2 [28] ( ) Fig. 2 Ten channel test strip structure diagram [28] (left for the internal structure, right for the external structure) 1 [20] Fig. 1 Immunochromatography reaction process and the test card [20] 3 上转换发光免疫层析技术在食品安全检测中的应用 3.1 食源性致病菌的快速检测,,, [26] Listeria monocytogenes (LM) p60,, LM-UPT-LF, LM-UPT-LF,,, [27] 200~300 nm,, [29] UCP,, AFM1-UPT-LF, AFM1, 20 min, 100%, 85%,,,,,,,,, lgg UPT, UCP,, IgG,,, 1:10, : (1),,, ; (2), ELISA,

4 5, : 1861 ; (3),, ; (4),,, 3.2 农兽药残留的快速检测, UPT [30] PAA-UCNPs,, 1 ng/ml, 1~10000 ng/ml 3 7, : 4 4 4,,,, UPT, 4 面临的问题与展望 1995 UPT,, 2006,,, [31-35],,, [36],, :, N- [37],, [38],, [39,40], [41],,,, UCP,,, 参考文献 [1],,,. [J]., 2012, 3(6): Jia GC, Zhi AM, Song CM, et al. Up-converting phosphor technology and its application in the field of food safety detection [J]. J Food Saf Qual, 2012, 3(6): [2] Panzenhagen PH, Aguiar WS, Gouvêa R, et al. Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS [J]. Food Addit Contam, 2016, 3(1): 1 5. [3] Zhang L, Huang YJ, Wang JY, et al. Hierarchical flowerlike gold nanoparticles labeled immuno-chromatography test strip for highly sensitive detection of Escherichia coil0157:h7 [J]. Langmuir, 2015, 31: [4] Oguri S, Yoneya Y. Assay and biological relevance of endogenous histamine and its metabolites: application of microseparation techniques [J]. J Chromatogr. B, 2002, 781(1 2): [5] Wang FM, Li W, Wang JS, et al. Detection of telomerase on upconversion nanoparticle modified cellulose paper [J]. Chem Commun, 2015, 51: [6],,,. [J]., 2014, 5(7): Tang YW, Gao ZY, Wei LQ, et al. Progress on immunolabelling chromatographic techniques in food safety detection [J]. J Food Saf Qual, 2014, 5(7): [7] Nyk M, Kumar R, Ohulchanskyy TY, et al. High contrast in vitro and in vivo photoluminescence bioimaging using nearinfrared to near infrared up-conversionin Tm 3+ and Yb 3+ doped fluoride nanophosphors [J]. Nano

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<4D6963726F736F667420576F7264202D203631372D3632312032303133303430333030312DBACEC0F25FD0A3B6D4B8E55F2DB6FED0A32D2D2DC8A5B5F4CDBCD6D0B5C4BBD8B3B5B7FBBAC52E646F63> 第 4 卷第 2 期食品安全质量检测学报 Vol. 4 No. 2 2013 年 4 月 Journal of Food Safety and Quality Apr., 2013 何莉 *, 姜笑寒 ( 广东省食品药品职业技术学校, 广州 510663) 摘要 : 本文通过科学地运用 html5+jsp+sql 技术, 建