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1 4 1 Vol. 4 No Journal of Food Safety and Quality Feb., 2013 朱小红 *, 牟宵, 刘海静 (, ) 摘要 :,,, 关键词 : ; ; ; Standard formulation of the veterinary drug residues in catering service and its application ZHU Xiao-Hong *, MOU Xiao, LIU Hai-Jing (Shaanxi Institute for Food and Drug Control, Xi an , China) ABSTRACT: In this paper, veterinary drug residues, varieties of food products and maximum residue limits which were relevant to veterinary drug residue standards in catering service industry were analyzed successively. The main categories of food which were supposed to be monitored in catering service were listed. Additionally, the detection methods of veterinary drug residues in raw aquatic products, ready-to-eat meat, dairy products and the differences of maximum residue limits between china and other countries were overviewed. Finally the foreground of on site rapid detection technique of veterinary drug residues in catering service was prospected. KEY WORDS: catering service; veterinary drug residues; maximum residue limits(mrl); rapid detection 1 引言 20, ( ),,, ( ),, (FAO/WHO), ( ),,, [1],,,, [1], 6 : :,, ; : ; : * 通讯作者 :,, zzxh@yahoo.com.cn *Corresponding author: ZHU Xiao-Hong, Vice Director Pharmacist, Shaanxi Institute for Food and Drug Control, No.421, Zhuque Road, Yanta District, Xi an , China. zzxh@yahoo.com.cn

2 190 4 ; : ; : ; : β2- ( ) [2],,,,,,, 2 餐饮服务环节兽药残留主要拟检测品种 2.1 生食水产品兽药残留的监测 ( ) ()( ),,, [3], DB11/ , DB35/ , ( 1) 1998,,, 熟肉制品兽药残留的监测, 235, [4], 噁 [3], 3 4 表 1 生食水产品兽药残留限量值及检测标准 Table 1 Analytical methods and MRL of veterinary drug residues in raw aquatic products (μg/kg) SN/T 1604 SN/T 1627 GB/T GB/T GB/T GB/T ( ) 100 GB/T 噁 300 GB/T 表 2 各国生食水产品兽药残留限量值比较 Table 2 Comparison of MRL of veterinary drug in raw aquatic products (μg/kg) 噁

3 1, : 191 Table 3 表 3 肉制品兽药残留限量参考值及检测方法 Analytical methods and MRL of veterinary drug residues in meat products (μg/kg) NY/T SN/T / SN/T 1604 GB/T 噁 GB/T 表 4 各国肉制品兽药残留限量值比较 Table 4 Comparison of MRL of veterinary drug in meat products (μg/kg) () 噁 () 2.3 乳制品的兽药残留监测,,, 235, [3], 5,, 6 3 兽药残留快速检测方法 (maximum residue limits, MRLs), [5],,,, :,,,,,, 5% [6], (HPLC),,,,, HPLC,,,,, [7]

4 192 4 表 5 乳制品兽药残留限量参考值及检测方法 Table 5 Analytical methods and MRL of veterinary drug residues in dairy products (μg/kg) SN/T 1604 GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T Table 6 表 6 各国乳制品兽药残留限量值比较 Comparison of MRL of veterinary drug in dairy products (μg/kg)

5 1, : 免疫技术酶联免疫吸附方法 (enzyme linked immunosorbent assay, ELISA) [5] (ELISA), ELISA β-,,, ELISA () ( ) [8],,, ELISA [9] GC-MS 2~3 h, GC-MS 30 min [8], ELISA : 95%, ;,, ; [9] 试纸法,, ( ), (),, [10],,, ;,, [9], 3~5 ng/ml [10,11] 3.2 生物传感器, [9],,,,, 1% [7] (surface plasmon resonance, SPR) G [2],, (SPR), [9] 3.3 其他快速检测方法,,, [12],,,, [12],, Q67 T3, [13], 10 Q67 2 T3,,, [13],,

194 4 4 结论,,, 参考文献 [1],,,. [J]., 2001, 22 (1): 88-91. Chen ZL, Yang Gx, Sun YX, et al. Veterinary drug residue toxicity and ecological toxicology research progress [J].

6 结论,,, 参考文献 [1],,,. [J]., 2001, 22 (1): Chen ZL, Yang Gx, Sun YX, et al. Veterinary drug residue toxicity and ecological toxicology research progress [J]. J South China Agr Univ, 2001, 22 (1): [2],,,. [M]. :, Wu YN, Shao B, Shen JZ, et al. Veterinary drug residue detection monitoring technology [M]. Beijing: Chemical Industry Press, [3] DB11/ [S]. DB11/ Hygiene Requirements for Eating Raw Aquatic Products Beijing local standards [S]. [4] 235.[EB]. tm Department of Agriculture Announced no The animal food in the highest of veterinary drug residue limits [EB] [5],. [J]., 2008, 8: He GZ, Long M. Meat food residue of veterinary drug fast detection research progress [J]. Meat Ind, 2008, 8: [6],,. [M]. :, Shi QY, Ji QX, Xu LY. Food safety rapid detection technology and application [M]. Beijing: Chemical Industry Press, [7],,. [M]. :, Wang L, Wang J, Zhou JY. Food safety rapid detection technical manual [M]. Beijing: Chemical Industry Press, [8],,,. ELISA [J]., 2008, 44(1): Fan GY, Wang JH, Wang ZL, et al. Development and application of competitive ELISA kit for rapid detection of strept omycin residues [J]. Chin J Vet Med, 2008, 44(1): [9],,,. [J]., 2009, (9): Zhang GP, Zhi AM, Deng RG, et al. Immunological rapid test of animal drug residues [J]. J Henan Agr Sci, 2009, (9): [10],,,. [J]., 2004, 24(1): Wang XN, Yang YY, Xing GX, et al. Development of rapid detection of clenobuterol hydrochloride single resistance [J]. Chin J Vet Med, 2004, 24(1): [11],,,. [P]:, ZL Zhang GP, Li XW, Wang XN, et al. Fast detection dipsticks of clenobuterol hydrochloride [P]: China, ZL [12],,,. [J]., 2003, 12: Du ZJ, Wang XH, Li HF, et al. The research and application of luminescent bacteria [J]. High Technol Lett, 2003, 12: [13],. [D]., Shi Y, Ding W. Luminescent bacteria rapid detection of veterinary drug residue animal research [D]. Northwest Agriculture and Forestry University, ( 责任编辑 : 赵静 ) 作者简介朱小红,, zzxh@yahoo.com.cn

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<453A5CCAB3C6B7BFC6D1A73131C4EA3134C6DA5C3134C6DA5C3032B7D6CEF6> 227 ELISA 刘沙洲 1,2, 桑小雪 1, 欧阳华学 3, 雷绍荣 3, 白林含 1, * (1. 四川大学生命科学学院, 四川成都 610065;2. 成都市食品药品检测中心, 四川成都 610045; 3. 四川省农业科学院分析测试中心, 四川成都 610066) 目的 : 比较