Microsoft Word 王似锦-二校 681

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1 6 2 Vol. 6 No Journal of Food Safety and Quality Feb, 2015 VIDAS 王似锦 *, 高春 ( ( ), ) 摘要 : 目的 (VIDAS) 方法 21 BPW BPW, VIDAS, 结果 21, BPW ; BPW 20 VIDAS GB, 95.2% 结论 VIDAS 96 h, VIDAS 48 h VIDAS,, 关键词 : ; ; ; Fast detection of Salmonella in health food by VIDAS method WANG Si-Jin *, GAO Chun (Beijing Institute for Drug Control (Beijing Center for Health Food and Cosmetics Control), Beijing , China) ABSTRACT: Objective The automated immunoassay analyzer (VIDAS) was applied to detect the Salmonella in health food. Methods The 21 lots of health food were divided into BPW group and BPW positive control group. The Salmonella in 21 lots of health foods were detected by both VIDAS method and GB method. The results were compared. Results All the results were negative in BPW group. Results of both VIDAS method and GB method in 20 lots of health food were consistent with each other in BPW positive control group, and the percentage accounted for 95.2% of the total. Conclusion VIDAS method could be used to fast detect the Salmonella in health food. It took 96 h at least to detect Salmonella by GB method. However, it took only 48 h by VIDAS method. It could be the final result if the result was negative, and it should be confirmed according to the GB method if the result was positive. KEY WORDS: health food; Salmonella; automated immunoassay analyzer; fast detection 1 引言 GB ( ) [1],, GB [2], 4 (96 h) VIDAS( ) (ELFA), (Solid Phase Receptacle),,, nm,, * 通讯作者 :,,, sijinw@163.com *Corresponding author: WANG Si-Jin, Responsible Pharmacist, Beijing Institute for Drug Control (Beijing Center for Health Food and Cosmetics Control), No.13, Shuiche Hongtong, Xicheng District, Beijing , China. sijinw@163.com

2 678 6,,, VIDAS 2 (48 h) [3,4], VIDAS, VIDAS,, 2 材料与方法 2.1 仪器与试剂 VIDAS( ) (SLM- Ⅱ ), (VITEK2- Compact)); (BPW) (TTB), (SC), (BS), (XLD) ; 2.2 样品 1, 方法 GB ,, (BPW), (CMCC(B)50094)50~100 CFU(BPW ) GB , (TTB) 24 h, VIDAS 3 结果与分析 2, 21 GB VIDAS, 21 BPW, GB 5, 表 1 样品列表 Table 1 The sample list 1 71 A K 2 77 B C 3338H7P D TAG E F G E 8 96 H I K05A J K 13K22B01B L JA M N O DJ P Q TAK R Z3A S T E U

3 2, : VIDAS 679 Fig. 1 1 Flow chart for detection of Salmonella in health food 16 ; VIDAS 4, 17, 20, 4, 16, 1, 77( B) 96( H) 109( P) 114( Q), 4, 4, BPW 3,,, [5-8] : 4, B [9], H [10] [11], P [12], Q [13] BPW, 1, 104( L), GB ( ), VIDAS,, VIDAS,, VIDAS [14-16] VIDAS, GB GB,,,,,, 21, VIDAS 48 h, GB 96 h~120 h, VIDAS

4 680 6 Table 2 表 2 保健食品 BPW 阳性对照沙门氏菌检验结果 Results of detection of salmonella (positive control) in health food BPW BPW GB VIDAS GB VIDAS 结论 21, 20 VIDAS GB, 95.2%, VIDAS, 48 h,, GB 参考文献 [1]. GB ( ) [S]. :, The people's Republic of China Technology Supervision Bureau. GB General standard for health (functional) foods [S].Beijing: Standards Press of China, [2]. GB [S]. :, Minister of Health of the People's Republic of China. GB National food safety standard Food microbiological examination: Salmonella[S]. Beijing: Standards Press of China, [3],. mini-vidas GB [J]., 2003, 22(1): Huang L, Meng DL. A comparative test of using Mini-Vidas and GB method inspect Slamonlla spp. for the food [J]. J Xinjiang Norm Univ (Nat Sci Edit), 2003, 22(1): [4],,,. [J]., 2010, 38(10): Huang CJ, Huang XR, Zheng J, et al. Detection of Salmonella in food samples by VIDAS method [J]. J Anhui Agri Sci, 2010, 38(10): [5],,,.

5 2, : VIDAS 681 [J]., 2013, 23(14): Wang SJ, Liu WJ, Sun W, et al. Classification of validation method in microbiological test of health food[j].chin L Health Lab Tec, 2013, 23(14): [6],,,. [J]., 2014, 24(13): Wang SJ, Zhang GH, Liu WJ, et al. Comparison of GB method and medium dilution method in aerobic plate count(apc) determination in health food [J]. Chin J Health Lab Technol, 2014, 24(13): [7],,,. ( ) [J]., 2008, 22(7): Te YX, Wang WJ, Qi WJ, et al. Discussion on the method of microbiological examination for health (funcitional) foods hygiene [J]. Chin Pharm Aff, 2008, 22(7): [8],,,. [J]., 2008, 28(2): Te YX, Gu T, Yang HS, et al. Preliminary study on antibacterial activity and mechanism of propolis [J]. Chin J Pharm Anal, 2008, 28(2): [9],,,. [J]., 2009, 34(5): Zhou XH, Zhang XX, Zhu XL, et al. Validation of method in microbial limit tests for health foods [J]. Food Sci Technol, 2009, 34(5): [10],,. [J]., 2009, 19(5): Lu XQ, Zhang CY, Wang B. Inhibitory effects of water first extractions of tea on digestive bacteria [J]. Chin J Nosocomiol, 2009, 19(5): [11],,. [J]., 2011, 11(6): Jin EH, Wu YY, Tu YY. Study on antibacterial effect of the aflavins [J]. J Chin Food, 2011, 11(6): [12],,. [J]., 1999, (9): Gong X, Yao SM, Lu YF. Study on antibacterial effect of extract of Ginkgo biloba [J]. Food Sci, 1999, (9): [13],,. [J]., 2006, 27(7): Pazilat B, Wen XM, Abdulla A. Extraction and bacteriostatic activities of Chromo-cor and Saccharides from Rhodiolarosea [J]. Food Sci, 2006, 27(7): [14],,,. VIDAS-VITEK O157 [J]., 2008, 18(8): Lin T, Lan M, Feng XJ, et al. Fast detection of Escherichia Coli O157 in foods by Vidas-vitek [J]. Chin J Health Lab Technol, 2008, 18(8): [15],. VIDAS O157 [J]., 2010, 12(2): Liu P, Shen JL. Preliminary screening and confirmation of suspected positive Escherichia Coli O157 by mini-vidas [J]. J Clin Transfus Lab Med, 2010, 12(2): [16],,,. - [J]., 2008, 34(8): Lin T, Lan M, Huang W, et al. Application on VIDAS-VITEK to detect Salmonella spp. in aquatic products [J]. Food Ferment Ind, 2008, 34(8): 作者简介 ( 责任编辑 : 白洪健 ) 王似锦, 主管药师, 主要研究方向为药品保健食品和化妆品的微生物检验, 致病菌的分离与鉴定 sijinw@163.com

Microsoft Word _刘冬玲-二校

Microsoft Word _刘冬玲-二校 7 3 Vol. 7 No. 3 2016 3 Journal of Food Safety and Quality Mar., 2016 刘冬玲, 蒋 * 波, 陈奇知, 杨美成 (, 201203) 摘要 : 目的方法 GB16740-2014, GB4789, 10 结果, 3 :, ;, 4, 结论,, 关键词 : ; ; ; Discussion on the method of microbiological