Microsoft Word 翟晓瑞-二校

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1 7 6 Vol. 7 No Journal of Food Safety and Quality Jun., 2016 M 1 翟晓瑞 *, 李燕 ( ( ), ) 摘要 : M 1 (aflatoxin M 1, AFM 1 ),, - - AFM1,,, AFM1 关键词 : M 1 ; ; Research progress of detecting methods of aflatoxin M 1 in dairy products ZHAI Xiao-Rui *, LI Yan (Mengniu Dairy (Taiyuan) Co., Ltd., Shanxi , China) ABSTRACT: Aflatoxin M 1 has strong carcinogenicity and mutagenicity, and mainly exists in meat, urine, milk, liver, kidney, muscle and blood of the animal, especially in milk. This paper reviewed the research progress of detecting methods of aflatoxin M 1 in dairy products, including thin layer chromatography, effective thin layer chromatography, high performance liquid chromatography, liquid chromatography-tandem mass spectrometry, enzyme-linked immunosorbent method, radio immunity method, immune colloidal gold method, electrochemical method, chemiluminescence method, and so on, outlined the advantages and disadvantages, and gave the prospect of detection methods in the future, which provided a technical support to guarantee the dairy safety and improve the detection ability of aflatoxin M 1. KEY WORDS: aflatoxin M 1 ; dairy product; detecting method 1 引言 M 1 (aflatoxin M 1, AFM1) Alleroft 1963, 1965 AFM1 [1,2] C 17 H 12 O 7, 328.6, 299,,, [3] 365 nm,,, 34 AFM μg/kg [4],, AFM1 0.5 μg/kg [5,6] AFM1,,, B [7] 1, DNA, [8-11], * 通讯作者 :,, liyan1983.love@163.com *Corresponding author: ZHAI Xiao-Rui, Master, Mengniu Dairy (Taiyuan) Co., Ltd., Tang Huai South Road, Xiaodian Distict, Shanxi , China. liyan1983.love@163.com

2 AFM1 [12,13] 2 检测方法 2.1 色谱法薄层色谱法 (thin layer chromatography, TLC) AFM1,, [14] AFM1,, 365 nm, AFM1,,, [15] 高效薄层层析法, 5~10 μm,, [16] [17],,,,, 高效液相色谱法 (HPLC) AFM1,,, [18,19] AFM1,,, AFM1 [20,21],, [22] AFM1 50%,,,, [23] 10% 25% 1 ml AFM1,,,,,, AFM1 0.1~1 μg/l, 90%~110%, 5 pg, 2 pg [24] - AFM1, 0.04 μg/kg, 0.10 μg/kg, %~2.56%,, 30 min, [25] 0.01 μg/kg,, 89.1%~93.5%, 6.5%~9.7%, AFM1, AFM 液相色谱 - 质谱联用法 - (LC-MS),,,, AFM1 [26] -,,,, [27] - AFM1, μg/kg, 0.01 μg/kg, 55, 20%, 2.2 免疫化学法免疫亲和柱 - 荧光光度法 20 90,,, AFM1, AFM1,, [28-30],, [31] Scott [32] 0.05 μg/l, 97%,, AFM 酶联免疫吸附法 20 70, 70 [33] AFM1 EIA, AFM1,, AFM1,,, 450 nm,,, AFM1 [34],, [35]

3 6, : M [36] AFM1, ng/l, RSD 0.58% 0.33%, 97.4% 94.1%, RSD 1.7% 1.2%,,, AFM1 [37] 4 AFM1, μg/kg, 5%, 93%~120%, 10%,, AFM 放射免疫法, AFM1, 3 H,,, 1 min,, AFM1,,, AFM1 [38],,,, [39] 1981 Pestka [40] AFM1, 5.0~50.0 μg/kg, 1.0 μg/l 免疫胶体金法 20 80,,, 90.5%, 100% [41], AFM1,, [42] EDC/NHS AFM1,,, 0.1 μg/l, AFM1 [43], AFM1 0.3 μg/l, 10 min, AFM1 2.3 电化学方法 20 90,, AFM1, [18] Parker [44] AFM1 8 ng/l, 10~100 ng/l 2.4 化学发光法, AFM1, AFM1,, AFM1, AFM1 [15] Magliulo [45] AFM1,, 1 μg/kg, 96%~122% 3 展望,,,,,, [46],, -,,,,,, -,,,,, AFM1 : (1), AFM1 ; (2), ; (3) AFM1, AFM1 参考文献 [1],. [M]. :, Wen YM, Lu DY. Modern microbiology [M]. Shanghai: Shanghai Medical University Press, [2]. M1 [J]., 1992(3): Nie J. Research progress of aflatoxin M 1 [J]. Domest Foreign Med Sci, 1992(3): [3],,,. M 1 [J]., 2004, 14(3): Zhang DS, Zhao XL, Zhao CC, et al. Advancement in researches of

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Microsoft Word - 3098-3102 20150611007-蔡伟江-二校

Microsoft Word - 3098-3102 20150611007-蔡伟江-二校 6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug., 2015 蔡伟江 *, 邓鹏飞, 刘光兰, 李丹 (, 519040) 摘要 : 目的, 方法 (4.6 mm 250 mm, 5.0 μm) ; : : : =70: 16: 14(V:V:V),, : 1.0 ml/min; : 40 ; : 10