中文摘要

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派弋煜戚
7 years ago
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1 DOH91-DC

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4 ABSTRACT Three outbreaks of adenovirus (Ad) respiratory infection were found during Nov to Dec in southern Taiwan via a surveillance program. To elucidate the serotypes and their molecular epidemiology as well as their association with the clinical features, a total of 526 virus strains were randomly selected from 1064 strains isolated during and were studied using restriction fragment length polymorphism (RFLP) and Polymerase chain reaction (PCR) -RFLP. The major subgenus found was subgenus B (45.1%), followed by subgenus E (29.3%) and C (25.5%). Ad3 and Ad7 were the major types found during the 1 st outbreak from Nov to March 2000, while Ad4 was found mainly in the 2 nd and 3 rd outbreaks in October 2000 and September Ad7 and Ad4 emerged in 1999 and 2000 and declined in 2001, respectively. Ad3 was constantly isolated during the surveillance, however it declined drastically from more than 36% to only 2% in Genotype analysis showed that Ad7a was found in 1983, however, only Ad7b was found in The clinical features of 218 patients were analysed during the outbreak. About 79% of total cases, their age were less than 7 years old. The ratio of male to female was 2:1. 4

5 Severe infections, such as pneumonia and acute bronchitis, account for nearly half of the cases (42.6%). The results reveal the reemerging, shifting of serotypes and the clinical association of respiratory adenovirus infections as well as the molecular epidemiology of Ad7 genotypes in southern Taiwan during recent two decades. Keywords: Adenovirus, Molecular Epidemiology and RFLP 5

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14 1. Abzug MJ, Levin MJ. Neonatal adenovirus infection: four patients and review of the literature. Pediatrics 1991; 87: Azar R, Varsano N, Mileguir F, Mendelson E. Molecular epidemiology of adenovirus type 7 in Israel: identification of two new genome types, Ad7k and Ad7d2. J Med Virol 1998; 54: Carballal G, Videla C, Misirlian A, Requeijo PV, Aguilar M. Adenovirus type 7 associated with severe and fatal acute lower respiratory infections in Argentine children. BMC Pediatr 2002; 16; 2(1): 6 4. Chang CH, Sheu MM, Chern CL, Lin KH, Huang WL, Chen CW. Epidemic keratocinjunctivitis caused by a new genotype of adenovirus Type 8 (Ad8)-a chronological review of Ad8 in Southern Taiwan. Jpn J Ophthalmol. 2001; 45: Chang CH, Sheu MM, Lin KH, Chen CW. Hemirrhagic viral keratoxonjunctivitis in Taiwan caused by adenovirus types 19 and 37: Applicability of polymerase chain reaction-restriction fragment length polymorphism in detecting adenovirus genotypes. Cornea 14

15 2001; 20(3): Chroboczek J, Bieber F, Jacrot B. The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2. Virology 1992; 186: Cooper RJ, Hallett R, Tullo AB, Klapper PE. The epidemiology of adenovirus infections in Greater Manchester, UK Epidemiol Infect 2000; 125: Douglas DR, Richard JW, Frederick GH. Adenovirus. Clinical virology. 1997; Erdman DD, Xu W, Gerber SI, Gray GC, Schnurr D, Kajon AE, Anderson LJ. Molecular epidemiology of adenovirus type 7 in the United States, Emerg Infect Dis 2002; 8(3): Hong JY, Lee HJ, Piedra PA, Choi EH, Park RH, Koh YY, Kim SW. Lower respiratory tract infections due to adenovirus in hospitalized Korean Children: epidemiology, clinical feature, and prognosis. Clin Infect Dis 2001; 32: Houng HS, Liang S, Chen CM, Keith J, Echavarria M, Sanchez JL, Kolavic SA, Vaughn DW, Binn LN. Rapid type-specific diagnosis of adenovirus type 4 infection using a hexon-based quantitative 15

16 fluorogenic PCR. Diagn Microbiol Infect Dis 2002; 42(4): Kajon AE, Mistchenko AS, Videla C, Hortal M, Wadell G, Avendano LF. Molecular epidemiology of adenovirus acute lower respiratory infections of children in the south cone of south America ( ). J Med Virol 1996; 48: Kolavic-Gray SA, Binn LN, Sanchez JL, Cersovsky SB, Polyak CS, Mitchell-Raymundo F, Asher LV, Vaughn DW, Feighner BH, Innis BL. Large epidemic of adenovirus type 4 infection among military trainees: epidemiological, clinical, and laboratory studies. Clin Infect Dis 2002; 35(7): Li Q.G. and Wadell G. Analysis of 15 Different Genome Types of Adenovirus Type 7 Isolated on Five Continents. J Virol 1986; 60(1): Li Q.G., Zang QJ, Liu YH, and Wadell G. Molecular epidemoiology of adenovirus type 3 and 7 isolated form children with pneumonia in Beijing. J Med Virol 1996; 49: Li QG, Wadell G. Comparison of 17 genome types of adenovirus type 3 identified among strains recovered from six continents. J Clin Microbiol 1988; 26 (5):

17 17. Lin KH, Chow TY, Sheu MM, Huang WL, Chen CW. A Rapid and simple method for preparation of adenovirus DNA for restriction endonuclease cleavage studies. Kaohsiung J Med Sci 1986; 2: Nahmias AJ, Griffith D, Snitzer J. Fatal Pneumonia Associated With Adenovirus Type 7. Amer J Dis Child 1967; 114(1): Palomino MA, Larranaga C, Avendano LF. Hospital-acquired adenovirus 7h infantile respiratory infection in Chile. Pediatr Infect Dis J 2000; 19: Saito-Inagawa W, Oshima A, Aoki K, Itoh N, Isobe K, Uchio E, Ohno S, Nakajima H, Hata K, Ishiko H. Rapid Diagnosis of Adenoviral Conjunctivitis by PCR and Restriction Fragment Length Polymorphism Analysis. J Clin Microbiol 1996; 34: Sheu MM, Huang WL, Lin KH, Chen CW, Lin CC. A Molecular Study of Adenovirus type 8 isolated from viral conjunctivitis in the Kaohsiung Area during : A chronological comparison of subtypes by endonuclease cleavage analysis. Kaohsiung J Med Sci 1987; 3(5): Sheu MM, Lin KH, Huang WL, Chen CW. Adenovirus type 19 and 37 Isolated from viral conjunctivitis in the Kaohsiung area during 17

18 : Molecular epidemiological study by DNA endonuclease cleavage analysis. Kaohsiung J Med Sci 1988; 4(2): Suzuki N, Ueno T, Yemashita T, Fujinaga K. Grouping of adenoviruses and identification of restriction endonuclease cleavage patterns of adenovirus DNA using infected cell DNA: Simple and practical methods. Microbiol Immunol 1981; 25(12): Tai FH, Grayston, JY, Johnston, PB, Woolridge, RL. Adenovirus infections in Chinese army recruits on Taiwan. J Infect Dis 1960; 107: Tsai HP, Kuo PH, Liu CC, Wang JR. Respiratory viral infections among pediatric inpatients and outpatients in Taiwan from 1997 to J Clin Microbiol 2001; 39(1): Videla C, Carballal G, Kajon A. Genomic analysis of adenovirus isolated from Argentinian children with acute lower respiratory infections. J Clin Virol 1999; 14: Wadell G, Annika Allard, and John C.Hierholzer. general issues in microbiology. Chapter ; Wadell G, Cooney MK,Linhares A, da C, de Silva L, Kennet ML, Kono R, Fang RG, Lindman K, Nascimento JP, Schoub BD, Smith 18

19 CD. Molecular epidemiology of adenovirus: Global distribution of adenovirus 7 genome types. J Clin Microbiol 1985; 21: Wadell G. Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol 1984; 110: Yamadera S, Yamashita K, Akatsuka M, Kato N, Inouye S. Trend of adenovirus type 7 infection, an emerging disease in Japan. Jpn. J. Med. Sci. Biol1998; 51:

20 Subgenus and species 1981~ ~ Total B 3 21 (67.7) 22 (44) 27 (36.0) 58 (36.3) 4(1.9) 7 1 (3.2) 0 34 (45.3) 34 (21.3) 3(1.4) 237(45.1) 14 3 (9.7) 4 (8) 3 (1.9) 23(11.0) C (12.9) 9 (18) 8 (10.7) 10 (6.3) 39(18.6) 2 2 (6.5) 14 (28) 6 (8.0) 10 (6.3) 32(15.2) 134(25.5) D E 8 1(0.6) 1(0.2) 4 1 (2) 44 (27.5) 109(51.9) 154(29.3) Total

21 1999~2000 Age groups Subgenus and 3 species 4~7 years 8~11 years 12 B (36.2) (31.2) (1.4) C (7.3) (6.9) D (0.5) E (16.5) Total 71(32.6) 101(46.2) 35(16.1) 11(5.0)

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24 M bp 1.Adenovirus hexon gene PCR, Ad, M: 100 bp Ladder 24

25 M (1) Sty M M (2) Hae M M (3)Hinf M bp Adenovirus hexon gene PCR 酶 StyHae Hinf d M: X174 Hinc 25

26 M 7a 7b 7b 3-7 M 3. 酶 B am H Ad7 Lane 1:Ad 7a(1155/83), Lane 2:Ad 7b(2123/99), Lane 3:Ad 7b(1469/00),Lane 4:Ad 3-7(2165/99). M : molecular weight marker (Hind andx174 Hinc ) 26

27 subgenus B subgenus C subgenus D subgenus E serotype 3 serptype

530 解放军医学杂志 2013 年 7 月 1 日第 38 卷第 7 期 4 临床表现 4.1 潜伏期 3~8d 潜伏期末至发病急性期传染性最强 4.2 临床表现腺病毒感染后主要表现为隐性感染腺病毒急

530 解放军医学杂志 2013 年 7 月 1 日第 38 卷第 7 期 4 临床表现 4.1 潜伏期 3~8d 潜伏期末至发病急性期传染性最强 4.2 临床表现腺病毒感染后主要表现为隐性感染腺病毒急 Med J Chin PLA, Vol. 38, No. 7, July 1, 2013 529 指南与共识腺病毒感染诊疗指南全军传染病专业委员会, 新突发传染病中西医临床救治课题组 [ 关键词 ] 腺病毒感染, 人 ; 诊断 ; 治疗 ; 医疗准则 [ 文献类型 ] [ 中国分类号 ]