5156 2014 9 34 3. Ghrdin n 6. 20R- Rg3 J. 2008125 293-5. J. 2011 4 Wang Y Narsinh K Zhao L et al. Effects and mechanisms of ghrelin on cardiac microvascular endothelial cells in rats J. Cell Biol Int 2011 352 135-40. 5 Scares JB Leite-Moreira AF. Ghrelin des-acyl ghrelin and obestatin three pieces of the same puzzle J. Peptides 2008297 1255-70. 3257-12. 7. J. 2013 732232-3. 2013-09-23 赵慧玲 ) 1 王桂华施真孙秀梅 2 王月华 2 孙辉杜冠华 马春蕾 264003 CG BL-420F Ⅱ ECG P R ST T 3 10 P R ST T ECG 3 R972R969. 3 A 1005-9202201418-5156-05doi10. 3969/j. issn. 1005-9202. 2014. 18. 064 Effect of Guan xin ning injection Ge gen su injection and Deng zhan hua su injection on heart's electrical activity of anesthetized rats WANG Gui-HuaSHI ZhenSUN Xiu-Meiet al. Department of PhysiologyBasic CollegeBinzhou Medical CollegeYantai 264003 Shandong China Abstract Objective To observe the effects of Chinese traditional herbal injection Guan xin ning injectionge gen su injection and Deng zhan hua su injection for cardiovascular diseases on heart's electrical activity in anesthetized rats and confirm the effect of these Chinese medical injection by intraperitoneal relatively high dose on ECG. Methods Electrocardiogram Lead Ⅱwas recorded by BL-420F biofunction laboratory systemthe changes of the ECG Wave Pwave RSegment STwave T and the heart rate at different times after the peritoneal injection ipof Chinese traditional herbal injection with or without intraperitoneal were observed and calculated. Results The results showed that these three Chinese traditional herbal injections could induce the alterations of heart's electrical activity in anesthetized rats. Intraperitoneal after administration of 1 or10 times normal dosage and the heart rate was differentially retardedexcept for saline 10 times normal dosage groupthe wave P upside down Ge gen su injection the elevation or decrease of ST Segment the elevation of amplitude of R and T wave in every groupadditionallyand premature ventricular accidentally Ge gen su injection and Deng zhan hua su injection. The abnormal waves might appear in the severe cases indicating Chinese traditional herbal injection could induce abnormal electrical activity in the heart. Long-term administration could causes heart damage. The ECG alterations were related with the type of Chinese traditional herbal injection the dosage and time-course of administration. Conclusions Three blood-activating and stasis-dissolving injections could cause all kinds of ECG for the type of Chinese herbal medicine dosage and time-course of administration. Much more attention should be paid on the cardiac damages and the aimed measures should be given during Chinese medical injection in action. Key words Guan xin ning injectionge gen su injectiondeng zhan hua su injectionecg 81171233 200902008 ZR2010HM011 2010252 Taishan Scholar No. tshw20110515 1 2 1959- ASIC1a NMDAR 1976-
18 5157 1 2 10 42 10 15 3 P = 0. 001 20 5 10 15 20 25 30 1 1. 1 SPF Wistar 64 0. 001 0. 001 0. 002 0. 002 0. 003 280 ~ 350 g SCXK 20090001 1. 2 BL-420F 1 2 HR% 10 HR% Z13020779 10 15 P = 100 mg /2 ml 0. 009 P = 0. 000 HR% 200 ~ 400 mg / H20055928 P = 0. 005 P = 0. 000 10 20 mg /5 ml 10 ~ 20 mg / HR% 15 20 25 30 Z14021942 10 P 0. 000 H31021724 1. 3 2 HR% 15 20 30 3 6 P 0. 001 0. 000 0. 000 10 60 HR% 5 10 P = 0. 00110 0. 2 ml /100 g 10 P = 0. 03425 30 2 ml /100 g P = 0. 011 P = 0. 023 2E 10 64 4 16 HR% 10 10 15 30 P 10 8 2% 0. 031 0. 006 0. 005 10 5 HR% 5 BL-420F 10 15 P 0. 000 0. 002 0. 033 ECG HR% Ⅱ ECG 5 min 5 10 P 30 min ECG ECG 0. 000 10 HR% 1. 4 5 10 15 20 25 30 min P 0. 001 0. 000 0. 000 0. 001 10 HR% 10 ~ 20 min R ST T mv 5 10 P = ECG 1. 5 SPSS11. 0 T 2 P 0. 042 0. 008 0. 000 0. 001 0. 004 5 min 10 15 20 25 30 P 2. 2 30 min 10 15 10 HR% 10 5 10 15 20 0. 002 P = 0. 000 2. 3 10 ~ 20 R ST-T R ST-T T P = 0. 017 2. 1 1A C E 10 R G ST-T R ST T P = 0. 013 ST P>0. 05 25 30 T P = 0. 047 P = 0. 017 R P 0. 019 P = 0. 606 ST 0. 025 1B D F H 10 P = 0. 025R T 3 4 10 ~ 25 10 ST-T
5158 2014 9 34 2. 4 10 ~ 20 R ST T 5 2. 5 ECG 2 ST% P T 1 P 0. 036 0. 000 0. 016 8 min P T 2 R% P = P 1 P 1 9 0. 018 P = 0. 013 P = 0. 033 P 2 min 2 A T% P T 3 10 ~ 20 0. 38 0. 065 0. 157 10 2 A 8 min R% ST% T% ST 2 10 min ECG T 10 ST% 1 P 0. 012 0. 000 0. 011 T% 2. 6 10 ECG 3 P 0. 002 0. 000 T% P T 1 P = 0. 577 P T 6 15 R% 2 P 0. 898 0. 371 R% 1 min 30 ~ 40 s P P = 0. 015 3 P 1 15 10 10 2 min T 7 ST% T% P 0. 000 0. 000 R% T% P 0. 058 0. 128 10 ~ 20 2 0. 006 10 R% ST% T% 1 min 30 ~ 40 s T P 0. 228 0. 497 0. 79 1 10 ST% P = 1 2 15 min ECG A 2 9 min ECG P B 2 14 min ECG T 2 8 min ECG T 2 8 min ECG ST T 2 2 12 min ECG A 2 9 min ECG P ST B 2 14 min ECG P T 2 1 min ECG 2 15 min ECG T 3 10 ECG
18 5159 1 x±s n =8 5 min 10 min 15 min 20 min 25 min 30 min -3. 524±0. 422-5. 671±0. 781-5. 984±0. 88-7. 310±2. 361-8. 062±1. 034-10. 09±1. 98 0. 891±0. 056-0. 536±0. 043 2-2. 526±0. 897 2-5. 032±2. 037-5. 786±1. 726-6. 763±2. 743 4. 29±1. 59 2-2. 463±0. 065 1-8. 737±3. 014-10. 177±2. 150-14. 954±3. 045 1-14. 06±2. 191 1 1. 045±1. 010 2 1. 895±0. 757 2 0. 726±0. 033-1. 253±0. 483-3. 211±0. 893-1. 77±0. 516 1P<0. 05 2P<0. 01 2 10 x±s n =8 5 min 10 min 15 min 20 min 25 min 30 min 2. 535±0. 648 4 3. 067±0. 688 4 12. 198±1. 946 4 0. 890±0. 036 0. 072±0. 034 4. 292±0. 943-6. 271±1. 459-9. 523±1. 943-19. 526±2. 801 24-20. 032±4. 005 24-21. 753±4. 566 2-18. 210±2. 043 24-1. 952±1. 089 4-14. 554±2. 164 14-15. 737±2. 074 23-16. 897±2. 787-16. 924±2. 149-17. 069±2. 114 2-1. 755±0. 411 24-3. 541±0. 552 24-3. 612±1. 034 2-2. 013±0. 736 2-1. 015±0. 193-1. 275±0. 458 1P<0. 05 2P<0. 01 3P<0. 05 4P<0. 01 3 R ST-T x±s n =8 R mv ST mv T mv 0. 489±0. 042 0. 512±0. 048 0. 151±0. 003 0. 149±0. 016 0. 098±0. 004 0. 109±0. 007 0. 515 ±0. 045 0. 588±0. 047 0. 338±0. 063 0. 289±0. 037 0. 180±0. 026 0. 197±0. 031 0. 554±0. 068 0. 592±0. 065 0. 245±0. 046 0. 285±0. 036 0. 127±0. 011 0. 161±0. 009 1 0. 609±0. 065 0. 681±0. 058 0. 257±0. 031 0. 239±0. 028 0. 115±0. 013 0. 136±0. 016 1P<0. 05 4 10 R ST-T x±s n =8 mv R ST T 0. 460±0. 037 0. 483±0. 03 0. 179±0. 026 0. 184±0. 022 0. 115±0. 009 0. 123±0. 008 0. 539 ±0. 029 0. 627±0. 027 1 0. 261±0. 007 0. 288±0. 010 1 0. 149±0. 009 0. 195±0. 017 1 0. 598±0. 039 0. 627±0. 05 0. 260±0. 017 0. 297±0. 010 1 0. 148±0. 009 0. 185±0. 013 1 0. 573±0. 033 0. 611±0. 028 0. 296±0. 006 0. 316±0. 007 1 0. 095±0. 004 0. 104±0. 006 1P<0. 05 5 10 ~ 20 R ST T x±s n =8 R% ST% T% 10 R% ST% T% 4. 62±0. 47-1. 09±0. 68 12. 19±2. 03 5. 02±0. 99 2. 31±0. 70 7. 17±1. 35 14. 09±4. 07 1-14. 44±4. 33 1 9. 46±2. 57 16. 37±3. 73 1 10. 54±2. 39 14 30. 51±6. 91 23 6. 79±0. 90 16. 29±2. 67 2 26. 9±8. 13 4. 81±1. 48 14. 03±2. 08 2 24. 50±4. 38 2 11. 76±2. 09 1-3. 90±0. 85 1 18. 91±4. 63 6. 56±1. 55 6. 69±1. 47 14 9. 34±4. 14 1P<0. 05 2P<0. 01 3P<0. 05 4P<0. 01 3 ECG 5 6 3 4 ECG ECG 3 10 R T ST 6 10 60 ECG 10 2 10
5160 2014 9 34 7 8 10 ST T 10 ECG ECG 9 10 15 min 10 20 min 10 R T 4 ST 10 1 Shaw D Graeme L Pierre D et al. Pharmacovigilance of herbal medicine J. J Ethnopharmacol 20121403 513-8. 11 2 Kim EJ Chen Y Huang JQ et al. Evidence-based toxicity evaluation and scheduling of Chinese herbal medicines J. J Ethnopharmacol 201231 ECG 128861-86. T 8 min 1 10 3.. J. ECG P 2011393 67-73. T 10 4 Xiong F Wang H Geng KK et al. optimized preparation characterization T ST and biodistribution in heart of breviscapine lipid emulsion J. Chem 10 Pharm Bull 201058111455-60. 10 1 min 5 Anigbogu CN Isichei CV Ajuluchukwu JN. Blood pressure heart rate 1 min ECG cardiovascular reflexes and electrocardiographic changes in some hypertensive nigerians J. Niger J Physiol Sci 2012271 23-7. 3 ECG 200461 50-1. 7 ECG. J. 2012164 552-3. - 8. J. ECG 200863 510-2. ECG 3 J. 2012193 27-30. ECG 10 ~ 15 minecg 5 minecg 12 ECG ECG 6. 100 J. 9. 10. 295 J. 201072456-8. ECG 3 ~ 5 min 11. 2 min ECG 1 J. 201267 12-3. 12 Liu HX Wang SR Lei Y et al. Characteristics and advantages of traditional Chinese medicine in the treatment of acute myocardial infarction ECG J. J Tradit Chin Med 2011314 269-72. 2013-08-21 赵慧玲 / 曹梦园 ) okgood911 @ 126. com 0431-88923384 971 130061