P = SPF Wistar ~ 350 g SCXK BL-420

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1 Ghrdin n 6. 20R- Rg3 J J Wang Y Narsinh K Zhao L et al. Effects and mechanisms of ghrelin on cardiac microvascular endothelial cells in rats J. Cell Biol Int Scares JB Leite-Moreira AF. Ghrelin des-acyl ghrelin and obestatin three pieces of the same puzzle J. Peptides J 赵慧玲 ) 1 王桂华施真孙秀梅 2 王月华 2 孙辉杜冠华 马春蕾 CG BL-420F Ⅱ ECG P R ST T 3 10 P R ST T ECG 3 R972R A doi /j. issn Effect of Guan xin ning injection Ge gen su injection and Deng zhan hua su injection on heart's electrical activity of anesthetized rats WANG Gui-HuaSHI ZhenSUN Xiu-Meiet al. Department of PhysiologyBasic CollegeBinzhou Medical CollegeYantai Shandong China Abstract Objective To observe the effects of Chinese traditional herbal injection Guan xin ning injectionge gen su injection and Deng zhan hua su injection for cardiovascular diseases on heart's electrical activity in anesthetized rats and confirm the effect of these Chinese medical injection by intraperitoneal relatively high dose on ECG. Methods Electrocardiogram Lead Ⅱwas recorded by BL-420F biofunction laboratory systemthe changes of the ECG Wave Pwave RSegment STwave T and the heart rate at different times after the peritoneal injection ipof Chinese traditional herbal injection with or without intraperitoneal were observed and calculated. Results The results showed that these three Chinese traditional herbal injections could induce the alterations of heart's electrical activity in anesthetized rats. Intraperitoneal after administration of 1 or10 times normal dosage and the heart rate was differentially retardedexcept for saline 10 times normal dosage groupthe wave P upside down Ge gen su injection the elevation or decrease of ST Segment the elevation of amplitude of R and T wave in every groupadditionallyand premature ventricular accidentally Ge gen su injection and Deng zhan hua su injection. The abnormal waves might appear in the severe cases indicating Chinese traditional herbal injection could induce abnormal electrical activity in the heart. Long-term administration could causes heart damage. The ECG alterations were related with the type of Chinese traditional herbal injection the dosage and time-course of administration. Conclusions Three blood-activating and stasis-dissolving injections could cause all kinds of ECG for the type of Chinese herbal medicine dosage and time-course of administration. Much more attention should be paid on the cardiac damages and the aimed measures should be given during Chinese medical injection in action. Key words Guan xin ning injectionge gen su injectiondeng zhan hua su injectionecg ZR2010HM Taishan Scholar No. tshw ASIC1a NMDAR 1976-

2 P = SPF Wistar ~ 350 g SCXK BL-420F 1 2 HR% 10 HR% Z P = 100 mg /2 ml P = HR% 200 ~ 400 mg / H P = P = mg /5 ml 10 ~ 20 mg / HR% Z P H HR% P HR% 5 10 P = ml /100 g 10 P = ml /100 g P = P = E HR% P % HR% 5 BL-420F P ECG HR% Ⅱ ECG 5 min 5 10 P 30 min ECG ECG HR% min P HR% 10 ~ 20 min R ST T mv 5 10 P = ECG 1. 5 SPSS11. 0 T 2 P min P min HR% P = ~ 20 R ST-T R ST-T T P = A C E 10 R G ST-T R ST T P = ST P> T P = P = R P P = ST B D F H 10 P = R T ~ ST-T

3 ~ 20 R ST T ECG 2 ST% P T 1 P min P T 2 R% P = P 1 P P = P = P 2 min 2 A T% P T 3 10 ~ A 8 min R% ST% T% ST 2 10 min ECG T 10 ST% 1 P T% ECG 3 P T% P T 1 P = P T 6 15 R% 2 P R% 1 min 30 ~ 40 s P P = P min T 7 ST% T% P R% T% P ~ R% ST% T% 1 min 30 ~ 40 s T P ST% P = min ECG A 2 9 min ECG P B 2 14 min ECG T 2 8 min ECG T 2 8 min ECG ST T min ECG A 2 9 min ECG P ST B 2 14 min ECG P T 2 1 min ECG 2 15 min ECG T 3 10 ECG

4 x±s n =8 5 min 10 min 15 min 20 min 25 min 30 min ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P< P< x±s n =8 5 min 10 min 15 min 20 min 25 min 30 min ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P< P< P< P< R ST-T x±s n =8 R mv ST mv T mv ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P< R ST-T x±s n =8 mv R ST T ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P< ~ 20 R ST T x±s n =8 R% ST% T% 10 R% ST% T% 4. 62± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± P< P< P< P< ECG ECG ECG 3 10 R T ST ECG

5 ST T 10 ECG ECG min min 10 R T 4 ST 10 1 Shaw D Graeme L Pierre D et al. Pharmacovigilance of herbal medicine J. J Ethnopharmacol Kim EJ Chen Y Huang JQ et al. Evidence-based toxicity evaluation and scheduling of Chinese herbal medicines J. J Ethnopharmacol ECG T 8 min J. ECG P T 10 4 Xiong F Wang H Geng KK et al. optimized preparation characterization T ST and biodistribution in heart of breviscapine lipid emulsion J. Chem 10 Pharm Bull min 5 Anigbogu CN Isichei CV Ajuluchukwu JN. Blood pressure heart rate 1 min ECG cardiovascular reflexes and electrocardiographic changes in some hypertensive nigerians J. Niger J Physiol Sci ECG ECG. J J. ECG ECG 3 J ECG 10 ~ 15 minecg 5 minecg 12 ECG ECG J J ECG 3 ~ 5 min min ECG 1 J Liu HX Wang SR Lei Y et al. Characteristics and advantages of traditional Chinese medicine in the treatment of acute myocardial infarction ECG J. J Tradit Chin Med 赵慧玲 / 曹梦园 ) 126. com