绎 阴 元元 / 100102 MCAT MCAO 84~333 mg kg -1 167 333 mg kg -1 MCAT 84 167 mg kg -1 MCAT 27% 37% 84~333 mg kg -1 MCAT 21%~ 32% MCAO LDH 10%~ 12% MDA 28%~38% SOD 15%~36% GSH 14%~36% doi: 10.3969/j.issn.1674-3849.2013.01.016 12 [1~3] [4, 5] [6, 7] -9 [8, 9] [10~14] 2012-07-02 2012-08-21 元 2004-2006 10 - 元元 85
2013 绎 Vol.15 No.1 1. 1 MACT SD 190 g~210 gmcao SD 280 g~300 g ZCXX 2002-2003 2 5014989 5015997 80% 3 84 167 333 mg kg -1 3 6 12 6 0.5% CMC-Na 4 益 FeCl 3 6H 2 O TTC LD LDH MDA SODGSH 20050921 20050921 20050820 20050912 20050920 20050815 3 XTT AEG-220 Df-206 HZQ-C 722 400R Heraeus 2. 1 淤 8 MCAT 84 167 333 mg kg -1 84 167 333 mg kg -1 0.1 ml kg -1 2 0.5% CMC-Na 3 30 min 10 h 1 于 MCAT 350 mg kg -1 10% Tamura MCAT [15] 1.5 cm 1mm 2.5 mm 10 滋 L 50% [16] 30 min FeCl 3 盂 6 h 24 h Bederson [18] a. 30 cm 0 l b. 0 1 c. 0 1 d. 30 cm 1 4 榆 5 TTC 5 ml 4% TTC 1.5 ml 1 mol L -1 K 2 HPO 4 0.1 ml 37 益 30 min 2 淤 86
绎 3 60 min 于 MCAO 350 mg kg -1 10% CCAECA ICA CCA ECA ICA ECA ICA 0.5 cm 0.285 mm 18 依 0.5 mm 1cm 2 h 2h 0.5 h 36.5 益 ~37.5 益 CCA ECA ICA CCA 盂 24 h 10% LD LDH SOD MDAGSH 3 SPSS 13.0 One-way ANOVA LSD x 依 s 1. MCAT MCAT 6h 24 h 24 h MCAT MCAT 6 24 h 167 333 mg kg -1 MCAT P<0.05 P<0.01 24 h 84 167 mg kg -1 32% 24% 21% 37% 27% P<0.05 P<0.01 P>0.05 1 2. MCAO MCAO LD LDH 52% 34% P<0.01 167 mg kg -1 MCAO LD 12% P<0.05 P<0.05 167 333 mg kg -1 LDH 10% P<0.01 84 167 333 mg kg -1 MCAO LDH 10%~ 12% P<0.01 1 MCAT mg kg -1 n 6h 24h % 原 10 0.0 依 0.0 ** 0.0 依 0.0 ** 0.0 依 0.0 ** MCAT 原 10 3.4 依 0.6 3.0 依 0.6 4.8 依 1.0 84 10 2.6 依 0.6 * 2.4 依 0.5 3.0 依 1.1 ** 167 10 2.2 依 0.8 ** 2.2 依 0.4 ** 3.4 依 1.0 * 333 10 2.5 依 0.8 * 2.3 依 0.4 * 4.0 依 2.1 84 9 2.7 依 0.8 2.5 依 0.8 3.2 依 1.4 * 167 10 2.6 依 0.8 * 2.4 依 0.5 * 3.6 依 1.2 * MCAT * P<0.05 ** P<0.01 333 10 2.5 依 0.6 * 2.4 依 0.4 * 3.8 依 1.2 * 87
2013 绎 Vol.15 No.1 3. MCAO MCAO SOD GSH 7% 18% P<0.05 P<0.01 MDA 103% P<0.01 MCAO SOD 36% 26% 19% 25% 15% P<0.01 GSH 20% 14% 26% 28% 36% P<0.05 P<0.01 167 mg kg -1 SOD P<0.05 333 mg kg -1 GSH P<0.05 167 333 mg kg -1 MDA 30% 34% 28% 38% P<0.01 P<0.05 3 85% [19~21] [22] -10 [23] [2] Fe 3+ OH MCAT [24] 2 MCAO (mg ml -1 ) n LD mmol g -1 pro LDH U g -1 pro - 12 0.34 依 0.03 ** 6157 依 310 ** MCAO - 13 0.52 依 0.05 8241 依 617 167 15 0.55 依 0.10 7371 依 727 ** 333 13 0.48 依 0.12 7368 依 788 ** 84 14 0.54 依 0.14 7192 依 778 ** 167 11 0.44 依 0.08 *# 7336 依 627 ** 333 13 0.55 依 0.10 7440 依 562 ** MCAO * P<0.05 ** P<0.01 # P<0.05 3 MCAO mg ml -1 n SOD U g -1 pro MDA nmol mg -1 pro GSH mg mg -1 pro - 12 141 依 9 * 0.97 依 0.28 ** 47 依 3 ** MCAO - 13 132 依 12 1.98 依 0.65 38 依 7 167 15 180 依 14 **# 1.37 依 0.32 ** 46 依 4 ** 333 13 166 依 26 ** 1.29 依 0.38 ** 44 依 4 * 84 14 156 依 12 ** 1.60 依 0.38 48 依 5 ** 167 11 164 依 20 ** 1.40 依 0.46 * 49 依 6 ** 333 13 151 依 19 ** 1.24 依 0.25 ** 52 依 12 **# MCAO * P<0.05 ** P<0.01 # P<0.05 88
绎 MCAO Ca 2+ NO [25] [26] SOD GSH O 2- H 2 O 2 MDA PUFA [18] MCAT 24 h 4.79% 6 h 24 h 6h 24 h 167 333 mg kg -1 MCAT 84 167 mg kg -1 MCAT MCAO LDH MDA SOD GSH SOD 167 mg kg -1 9% P<0.05 GSH 333 mg kg -1 18% P<0.05 MCAO LD 167 mg kg -1 1,..,2012,7(2) 颐 155~156. 2,,,..,2011,17(23) 颐 188~191. 3,..,2011,9(5) 颐 574~576. 4,. 37.,2012,39(4) 颐 692~693. 5,,,. 24.,2011,20(10) 颐 1695~1696. 6,,,..,2010,35 (24) 颐 3333~3338. 7..,2012,21(2) 颐 294~295. 8..,2012,28(4) 颐 329~330. 9,,,..,2003,28(7) 颐 636. 10,,,..,2011,36(18) 颐 2562~2566. 11,,,. MMP -9 mrna., 89
2013 绎 Vol.15 No.1 2011,11(8) 颐 875~879. 12..,2005,21(3) 颐 12~14. 13,,,. ET NO.,2011,27(1) 颐 61~63. 14,,,..,2011,22(11) 颐 2639~2640. 15 Tamura A, Graham DI, McCulluoch J, et al. Focal cerebral is 原 chemia in the rat. Description of technique and early neuropatho 原 logical consequences following middle cerebral artery occlusion. J Cereb Blood Flow Metab, 1981, 1(1) 颐 53~60. 16,..,1995,30(5) 颐 662~667. 17 Bederson JB, Pitts LH, Tsuji M, et al. Rat middle cerebral artery occlusion evaluation of the mode and development of a neurologic examination. Stroke, 1986, 17(3) 颐 472~476. 18 Ikeda Y, Long DM. The molecular basis of brain injury and brain edema: The role of oxygen free radicals.neurosurgery,1990, 27 (1) 颐 1~11. 19..,1993,25(12) 颐 33~34. 20,,. 53.,1993(2) 颐 33~34. 21,..,1993,7(1) 颐 29. 22,,. 31.,1997,16(4) 颐 170~171. 23,,,..,2011,9(6) 颐 710~712. 24,,,..,2004,26(5) 颐 386~389. 25,,,..,2003,55(6) 颐 742~747. 26..,1999,42(12) 颐 704~705. Protective Effect of An-Gong-Niu-Huang-Wan (Containing Natural or Artificial Moschus) on Experimental Cerebral Ischemia in Rats Dong Shifen, Lou Liming, Zhang Shuofeng, Sun Jianning (School of Chinese Materia Medica, Beijing University of Chinese Medicine; Laboratory for Basic Chinese Materia Medica and New Drug Research, Beijing 100102, China) Abstract: This article was aimed to investigate the protective effects of An-Gong-Niu-Huang-Wan containing artificial moschus or natural moschus on experimental cerebral ischemic model rats. Cerebral ischemia was estab 鄄 lished by middle cerebral artery thrombosis (MCAT) or middle cerebral artery occlusion (MCAO) in rats. The neu 鄄 rological function, cerebral infarct volume, acidosis and free radical metabolism were observed in the MCAT or MCAO rats after intragastric administration with An-Gong-Niu-Huang-Wan containing artificial or natural moschus at the dosage of 84~333 mg 窑 kg -1. The neurological function and the infarct region was significantly ame 鄄 liorated after treated with two kinds of An-Gong-Niu-Huang-Wan at the dosage of 84 ~333 mg 窑 kg -1, when compared with non -treated MCAT rats. The lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) content were significantly decreased by 10% ~12% and 28% ~38% respectively, and superoxide dismutase (SOD) activity and glutathiose (GSH) content were significantly increased by 15%~36% and 14%~36% respectively in MCAO rats after intragastric administration with An-Gong-Niu-Huang-Wan containing artificial or natural moschus, when compared with non -treated MCAO rats. It showed no statistical significant difference when com 鄄 pare the effect of two kinds of An-Gong-Niu-Huang-Wan. It was concluded that An-Gong-Niu-Huang-Wan with either artificial or natural moschus treatment could attenuate experimental cerebral ischemic rats. And there were no significant differences on the effects of both types. Keywords: An-Gong-Niu-Huang-Wan, artificial moschus, natural moschus, middle cerebral artery thrombosis (MCAT), middle cerebral artery occlusion (MCAO) 90