60 6 harmful to the growth and metabolism of the fish. Key words: Jade perch ( Scortum barcoo) ; digestible carbohydrate; blood biochemical indices; g

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1 South China Fisheries Science Vol. 6, No. 2 Apr., 2010 doi: / j. issn , 2, ,,,, ( 1.,, ; 2., ; 3., ) : 0% 6%23%40% ( Scortum barcoo) 6 36 h,, ( 23% ) ;,, ( P < 0. 05),, ; ( 40% ),, 23%,, : ; ; ; : S 963 : A : ( 2010) Effects of dietary digestible carbohydrate level on postprandial metabolism of Jade perch Scortum barcoo QI Ye 1, SUN Xiangjun 2, YU Gang 3, LIANG Yongjun 2, SONG Liwei 1, BAI Dongqing 1, QIAO Xiuting 1 ( 1. Tianjin Key Lab. of Aqua-Ecology and Aquaculture, Fishery Science Department, Tianjin Agricultural University, Tianjin , China; 2. Beijing Fisheries Research Institute, Beijing , China; 3. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou , China) Abstract: Jade perches ( Scortum barcoo) were fed with iso-protein and iso-lipid experimental diets at digestible carbohydrate levels of 0% ( control group), 6% ( group), 23% ( group) and 40% ( group ) for 6 weeks. After 36-hour fasting, the blood biochemical indices and contents of liver and muscle glycogens were examined at 0, 3, 6, 12, 24 and 36 h, respectively. The results showed that the growth performance and feed utilization of group ( 23% ) were the best. Contents of plasma glucose, triglyceride and total cholesterol all increased firstly and then decreased after feeding; plasma glucose and triglyceride increased significantly fast ( P < 0. 05) with increasing digestible carbohydrate level in diets, while total cholesterol decreased gradually; total amino did not change obviously. Liver glycogen content ( except for group ) increased firstly and then decreased after feeding; muscle glycogen decreased firstly, then increased gradually and finally remained stable. Contents of liver and muscle glycogens increased faster with increasing level of digestible carbohydrate. The results indicated that the growth performance and feed utilization of Jade perch at 23% digestible carbohydrate a- chieved the best effect. Exorbitant digestible carbohydrate could cause physiological load and stress for the organism, which would be : ; : : ( ) ; ( Z ) : ( ), qiye0510@ 163. com :, qxt6510@ yahoo. com. cn

2 60 6 harmful to the growth and metabolism of the fish. Key words: Jade perch ( Scortum barcoo) ; digestible carbohydrate; blood biochemical indices; glycogen [ 5-8 ],, [ 1 ], [ 2], [ 3 ], [ 4] 1,,, 1. 1, ( Scortum barcoo),,, 0 ( ) 6% ( ) 23% ( ) 40% ( ), 4 ( 1), 2. 4, mm, MD-22 (, ),,, 1 Tab. 1 Formula and proximate composition of experimental diets % composition control group group group group casein fish meal glucose sucrose dextrin starch mcc corn oil premix choline Ca( H 2 PO 3 ) cmc nutrition composition : Note: crude protein crude lipid digestible carbohydrate Premix contains trace-minerals, vitamins, et al.

3 2 : 61, ( g) ( ) g , 12 ( g) ] / ( g) 100% 35, 0. 8 m 0. 8 m 1. 0 m, m 10 m 1. 2 m ) ] / 100% 1 /3, 24 h, = ( g) / ( g) % 8: 30 15: ( ) ph , > 7 mgl - 1, mgl h,, h 3 MS-222,, rmin min, - 80, ( P < 0. 05),, ;, ; - 80, ( P < 0. 05), AN- ( P < 0. 05), ALYTECH-738PLUS ;,, ( P >, 0. 05) ( 2),,, 100% = ( g) - =[ ( g) - =[ ln ( ) - ln ( SPSS ANOVA,, 42 d Duncan P < ,, 2 Tab. 2 Growth performance of Jade perch fed with different digestible carbohydrate levels control group group group group / g a a a a / g a a b a / g a a b c / % a ac b c / % a ac b c a ac b c 2. 2 ( P <0. 05) a h, 3 h, 6 24 h, 0 12 h, h h 0 6 h 12 h, 12 h, 6 h, 6 12 h, 12

4 h, h ( P <0. 05) mmol L - 1,, 0 3 h, 3 6 h ( P < 0. 05), 36 h, 6 h 6 24 h, 24 h h,, h, h h 36 h mg g - 1, ( 1 - b) ( P <0. 05) mmoll - 1, 3 ( P < 0. 05), ( P < 0. 05) 2. 4 [ 9 ] 0 6 h, 0 3 h ( P < 0. 05), 6 h, 6 36 h, 36 h ( 1 - c ) mmoll - 1,,, 2. 5, 0 6 h, 6 36 h 36 h ( 1 - d), 0 12 h, h, 36 h [ ],,,, e ( GK) 0 12 h, h, 12 h [ 1 3], GK 0 24 h, 24 h, h 2. 7 ( 1 - f) 0 6 h, 6 24 h, 24 h,,,,, [ 10 ],,, GK [ ], GK 0 3 h, 3 h 36 h 24 h 24 h,, , mgg - 1, ( P,, < 0. 05),, 3, ( P < 0. 05) 2 8 h [ 17],, 36 6 h, 12 h, h 4,,

5 2 : 63 1 ( a) ( b) ( c) ( d) ( e) ( f) Fig. 1 Effect of dietary digestible carbohydrate level on plasma glucose ( a), triacylglyceride ( b), total cholesterol ( c), total amino ( d), liver glycogen ( e) and muscle glycogen ( f) of Jade perch after feeding [ 14],,, [ 18 ] [ 1 9],, ( Erythroculter ilishaeformis),,,,,,,

6 64 6,,,, 3 h, [ ],,,, 3 h [ 34 ],,,, [ 3 5] 3 h ; PHILLIPS [ 20] 1948,,,,,, 23% [ ], ( 3 h ),, ;, [ , 27 ],,,,, :, [ 26], ( Carassius au- ratus gibelio) [ 24-25] 4 6 h, 3 h,,,,,,,,,, [ ],,, h,,, 36 h [ 33] [ 14] ( Ctenopharyngodon idellus) ( Silurus meridionalis), [ 1] HIAU S Y. Utilization of carbohydrates in warmwater fish - with particular reference to tilapia, Orechromis niloticus O. aureus [ J]. Aquac, 1997, 151 ( 1) : [ 2] WILSON R P, POE W E. Apparent inability of channel catfish to u- tilize dietary mono-and disaccharides as energy sources [ J]. Nutr, 1987, 117 ( 2) : [ 3] DIXON D G, HILTON J W. Influence of available dietary carbohydrate content on tolerance of waterborne copper by rainbow trout Salmo gairdneri Richardson [ J]. Fish Biol, 1981, 19 ( 5 ) : [ 4 ]. [ M]. : 1996: [ 5],. [ J]., 2004, 34 ( 3) : [ 6],,,. [ J]., 2004, 28 ( 4 ) : [ 7],,. [ J]., 2004, 30 ( 5 ) : 553 [ 8],,,. [ J]., 2008, 38 ( 4) : [ 9],,. [ J]., 2007, 28 ( 1) : 7-9. [ 10]. [ D]. :, [ 11] HARMON J S, RIENIETS L M, SHERIDAN M A. Glucagen and

7 2 : 65 insulin regulate lipolysis in trout liver by altering phosphorylation of triacylglycerol lipase [ J]. Am J Physiol, 1993, 265 ( 1 /2 ) : [ 12] EILERTSON C D, SHERIDAN M A. Differential effects of somatostatin-14 and somatostatin-25 on carbohydrate and lipid metabolism in rainbow trout, Oncorhynchus mykiss [ J]. Gen Comp Endocrinol, 1993, 92 ( 1) : [ 13] PANSERAT S, CAPILL E, GUTIERREZ J, et al. Glucokinase is highly induced and glucose-6 -phosphatase poorly repressed in liver of rainbow trout ( Oncorhynchus mykiss) by a single meal with glucose [ J]. Comp Biochem Physiol Part B, 2001, 128 ( 2) : [ 14],,. [ 28] WALTON M J. Metabolic effects of feeding a high protein/ low car- [ J]., bohydrate diet as compared to a low protein/ high carbohydrate diet 2006, 30 ( 3 ) : [ 15] PANSERAT S, MEDALE F, BLIN C, et al. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp [ J]. Am J Physiol Regul Integr Comp Physiol, 2000, 278 ( 5 ) : [ 16] MOON T W. Glucose intolerance in teleost fish: fact or fiction [ J]. Comp Biochem Physiol Part B, 2001, 129 ( 2 /3 ) : [ 17] HUTCHINS C G, RAWLES S D, GATLIN DM. Effects of dietary carbohydrate kind and level on growth, body composition and glycemic response of juvenile sunshine bass [ J]. Aquac, 1998, 161 ( 1 /4) : [ 18]. [ M]. : ( Oncorhynchus tshawytscha) of different strains in sea water [ J]. 1999: [ 19]. [ 32],,,. [ D]. :, [ 20] PHILLIPS S M, JR TUNSION A V, BROCKWAY, D R. Utilization of carbohydrates by trout [ J]. Fish Res Bull NY, 1948, 11: [ 21] HARMON J S, EILERTSON C D, SHERIDAN M A, et al. Insulin suppression is associated with hyper-somatostatimemia and hyperglucagonemia in glucose injected rainbow trout [ J]. Physiol, 1991, 261 ( 2 /3) : Am J [ 22] WILSON R P. Utilization of dietary carbohydrate by fish [ J]. Aquac, 1994, 124 ( 1 /4) : [ 23],,,. in gilthead seabream ( Sparus aurate) [ J]., 2005, l2 ( 4) : [ 24],,, [ J]., 2003, 27 ( 6 ) : [ 25],,,. [ J]., ( 3) : [ 26],,,. [ J]., 2008, 32 ( 4 ) : [ 27] PANSERAR S, PLAGNES-JUAN E, KAUSHIK S. Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp ( Cyprinus carpio) and gilthead seabream ( Sparus aurata) [ J]. Biochim Biophys Acta, 2002, 1579 ( 1) : to rainbow trout ( Salmo gairdneri) [ J]. 1986, 1 ( 1) : Fish Physiol Biochem, [ 29] BEAMISH F W H, HILTON J W, NIIMI E, et al. Dietary carbohydrate and growth, body composition heat increment in rainbow trout ( Salmo gairdneri) [ J]. Fish Physiol Biochem, 1986, 1 ( 2) : [ 30] KIM J D, KAUSHIK S J. Contribution of digestible energy from carbohydrates and estimation of protein energy requirements for growth of rainbow trout ( Oncorhynchus mykiss) [ J ]. Aquac, 1992, 106 ( 2) : [ 31] MAZUR C N, HIGGS D A, PLISETSKAYA E, et al. Utilization of dietary starch and glucose tolerance in juvenile chinook salmon Fish Physiol Biochem, 1992, 10 ( 4 ) : [ J]. : 1997, 15 ( 3) : [ 33],,,. [ J]. : 2002, 41 ( 3) : [ 34] DENG D F, REFSTIE S, HUNG S S S. Glycemic and glycosuric responses in white sturgeon ( Acipenser transmontnus) after oral administration of simple and complex carbohydrates [ J]. Aquac, 2001, 199 ( 1 /2) : [ 35] PERES H, GONCALVES P, OLIVA-TERES A. Glucose tolerance and European seabass ( Dicentrarchus labrax ) [ J]. Aquac, 1999, l79 ( 1 / 4 ) : 415 -

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Microsoft Word - 24 杨伟.doc 36 3 Vol. 36, No.3 2012 5 ACTA HYDROBIOLOGICA SINICA May, 2 0 1 2 DOI: 10.3724/SP.J.1035.2012.00563 杨伟叶继丹王琨孙云章张春晓翟少伟宋凯 (,, 361021) GLUCOSE TOLERANCE IN GROUPER (EPINPHELUS COIOIDES) YANG Wei, YE Ji-Dan,