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1 17 6 Vol.17 No Journal of Fishery Sciences of China November 2010 张娜, 罗国芝, 谭洪新, 孙大川, 潘云峰, 郑海粟 (, ) 摘要 : (Scortum barcoo)(107.52±0.87) g, d, :, , (P>0.05), (DWG)(SGR)(FCR) (FR) (P<0.05);,,,, (P>0.05); (P<0.05), 25~26, 28, [, 2010,17(6): ] : ; ; ; ; 中图分类号 : S96 文献标识码 : A 文章编号 : (2010) ,, [1],, [2], [3] (Siniperca chuatsi) (Ophicephalus argus Cantor), (Esox lucius) [4] 23 ; [5-6] (Scortum barcoo), [7-10],,, 1 1.1, 1, (107.52±0.87) g ( 44.0%, 5.0%, 18.0%, 1.00%~4.00%, 1.20%, 0.50%~3.00%, 收稿日期 : ; 修订日期 : 基金项目 : (S30701). 作者简介 : (1986 ),,,. zhangna @163.com 通讯作者 : (1981 ),,. [email protected]

05), 25~26, 28, [, 2010,17(6): 1236 1242] : ; ; ; ; 中图分类号 : S96 文献标识码 : A 文章编号 : 1005 8737 (2010)06 1236 07,, [1],, [2], [3] (Siniperca chuatsi) (Ophicephalus argus Cantor),

2 6 : %, 12.0%) 1.2 ( 1), 4, 2, 1,,, 24 h, ph 6.5~7.5, 5~6 mg/l 1 Fig. 1 Construction of Recirculating aquaculture system for Scortum barcoo : Culture tan; Bio-filter; Temperature control; Pump; Sand filter ~38 [11], 3, , 32 kg/m 3 6 h 1, 3 d, 28 d 9:00 5: , 10 (Specific growth rate) (Day weight gain) (Feed conversion ratio) (Feed ration), : (SGR) = 100(lnW 2 1nW 1 )/(t 2 t 1 ) (DWG) = (W 2 W 1 )/(t 2 t 1 ) (FCR) = F/[n(W 2 W 1 )] (FR) = 200F/[(W 2 +W 1 )n(t 2 t 1 )] = 100 ( / ), W 1 W 2 (g) (g), F (g), n d, 10, 200 mg L 1 MS-222,, r/min 20 min,, : (ALT): ( ) : ( ) : : :,,,, 1.4,, 10~11 h YSI(YSI Incorporated. Yellow Springs, Ohio USA) ph,, TAN NH 3 N NO 2 N 3 ( 1), ( 0.02 mg L 1 ) ph 6.5 NaOH,, ( ), 表 1 不同温度处理组中的水质情况 Tab. 1 Water indices of different temperatures x ±SD Water Indices ph 6.82± ± ±0.38 DO 6.02± ± ±0.46 Salinity 0.74± ± ±0.15 TAN/(mg L 1 ) 1.56± ± ±0.19 NH 3 -N /( g L 1 ) 4.20± ± ±0.49 NO 2 -N/(mg L 1 ) 0.12± ± ±0.01

1.3.1 10~38 [11], 3, 16 22 28 2, 32 kg/m 3 6 h 1, 3 d, 28 d 9:00 5:00 2 1.3.2, 10 (Specific growth rate) (Day weight gain) (Feed conversion ratio) (Feed ration), : (SGR) = 100(lnW 2 1nW 1 )/(t 2 t 1

3 ±( x ±SD), SPSS 16.0 (One Way ANOVA), (LSD) % SGR : 16~28,, SGR,, 28 SGR 22, SGR DWG (P<0.01) (0 7 d), 28 DWG, 16 DWG, (P< 0.05) ;, 22, DWG SGR 16 (P<0.05) FCR (P<0.05), 16 FCR ( 2 2) Treatment /g Initial BW 表 2 不同温度处理组中的宝石鲈生长情况 Tab. 2 Growth of Scortum barcoo cultured at different temperatures /g Final BW n=10 ; x ±SD DWG/(g.d 1 ) SGR/% FCR FR/% ±1.25 a ±1.04 a 1.07±0.04 a 0.86±0.035 a 0.72±0.08 a 0.62±0.04 a ±1.74 a ±1.42 c 1.95±0.05 b 1.45±0.030 b 1.11±0.05 b 1.59±0.10 b ±1.41 a ±1.53 b 1.87±0.04 b 1.44±0.050 b 1.09±0.01 b 1.56±0.05 b : (P<0.05), (P>0.05). Note: The different letters on the parameters in one row mean significant differences (P<0.05); the same ones mean no significant differences (P>0.05). Fig. 2 2 Body weight of Scortum barcoo under different water temperature treatments SGR (T) ( 3) : SGR= T+( 0.008)T 2 (R 2 = P<0.01), SGR, Fig. 3 3 Correlation between water temperature and SGR of Scortum barcoo (P<0.05)(P 0.01)( 1), (FR) ( 4): FR=

d 1 ) SGR/% FCR FR/% 16 108.80±1.25 a 138.65±1.04 a 1.07±0.04 a 0.86±0.035 a 0.72±0.08 a 0.62±0.04 a 22 108.75±1.74 a 163.35±1.42 c 1.95±0.05 b 1.45±0.030 b 1.11±0.05 b 1.59±0.10 b 28 105.00±1.

4 6 : 1239 Fig. 4 4 Correlation between water temperature and feed ration of Scortum barcoo 0.683T+( 0.014)T 2 (R 2 =0.979 P<0.01), ( 3), : 16~28,,, (P>0.05);, (P>0.05), 表 3 不同温度处理组中的宝石鲈血液生化免疫指标 Tab. 3 Biochemical and immunological parameters of Scortum barcoo under different temperatures n=10; x ±SD Treatment /(u L 1 ) ALT /(mol L 1 ) Triacylglycerol /(u L 1 ) Lysozyme /(ng L 1 ) Cortisol % Spleen weight index ±0.18 a 5.64±0.19 a 39.58±1.84 a 8.68±2.06 a 0.061±0.002 a ±0.33 a 5.82±0.29 a 41.73±1.30 a 14.50±1.53 a 0.072±0.004 a ±0.35 a 6.28±0.23 a 42.86±1.35 a 33.23±2.62 b 0.060±0.006 a : (P<0.05), (P>0.05). Note: The different letters on the parameters in the same column mean significant differences (P<0.05); the same ones mean no significant differences(p 0.05). (P>0.05);, (P< 0.01), (P>0.05) 4, % 0.060%, %,, (P>0.05) 3 3.1,,,,,,,, [12] [13] 30, (Anguilla mossambica) 30 Tomas Arnason [14], (Scophthalmus maximus) : 1g 10 g 100 g g , 16 28, (P<0.01), , 28 22,,,, [15],

3 Biochemical and immunological parameters of Scortum barcoo under different temperatures n=10; x ±SD Treatment /(u L 1 ) ALT /(mol L 1 ) Triacylglycerol /(u L 1 ) Lysozyme /(ng L 1 ) Cortisol %

5 (25.75 ) (24.39 ), [15] (), [16] [17] [18],,,,, 25~26,,,, [19],, [20], , 16,, 16,,, 22 28, 22, ( ) 3.2,,, [21] [22], (P>0.05),,,,,,, [23-25],,, [26],,, [27] [28] : 4~30,,,,,,, [29],,, [30], Roed [31] ; Mock [32] Yin [33],,,,,,,, [34-35] Chen [36] (4 ),,, Cataldi [35]

6 6 : 1241 Carine Luisa Lermen [37],, 4, 28 (33.23 ng ml 1 ) (P<0.05) (8.68 ng ml ng ml 1 ),, 28,, 参考文献 : [1]. [M]. :, 1999: [2],. [J]., 2008, 24(1): [3],,. [J]., 2002, 26(5): [4],,. [J]., 2006, 30(1): [5] Larsson S, Berglund I. Growth and food consumption of arctic char fed pelleted or natural food at six different temperatures[ J ]. Fish Biol, 1998, 52: [6] Ellis C S, WatanabeW O, Ellis E P. Temperature effects on feed utilization and growth of postsettlement stage Nassau groper[j]. Trans Amer Fish Soc, 1997, 126: [7],,,. 鯻 [J]., 2003, 12(2): [8],. [J]., 2004, 31(1): 25. [9],,,. [J]., 2005, 22(7): [10],,,. [J]., 2009, 36(2): [11]. [J]., 2008, 25(1): [12],. [J]., 1994, 18(2): [13],,. [J]., 2009, 37(2): [14] Tomas Arnason, Bjorn Bjornsson, Agnar Steinarsson, et al.effects of temperature and body weight on growth rate and feed conversion ratio in turbot (Scophthalmus maximus) [J]. Aquaculture, 2009, 295: [15],,,. [J]., 2005, 12(3): [16] Jobling. Fish Bioenergetics[M]. London: Chapman and Hall, [17] Imsland A K, Sunde L M, Folkvord A, et al. The interaction of temperature and fish size on growth of juvenile turbot[j].j Fish Biol,1996, 49: [18] Woiwode J G, Adelman I R. Effects of temperature, photoperiod, and ration size on growth of hybrid striped bass white bass[j]. Trans Am Fish Soc, 1991, 120: [19]. [J]., 1989, 13(4): [20] Diana J S. Biology and Ecology of Fishes[M]. New York: Biological Sciences Press, 1995: [21] Manning M J. Fishes [M]// TurnerR J. Immunology A Comparative Approach. Britain: John Wile & Sons Ltd, 1994: [22],,,. [J]., 2008, 28(3): [23]. [M]. :, [24] Berentd, Schmidth, Wahlit, et a1. Effluent from a sewage treatment works causes changes in serum chemistry of brown trout(salmo trutta L)[J]. Ecotox Environ Safe, 2001, 48(2): [25]. [J]., 1999, 6(1): [26],,. [J]., 2005, 24(1): [27] Wolfe R R, Klein S, Carraro F, et al. Role of triglyceride fatty acid cycle in controlling fat metabolism in humans during and after exercise[j]. Am J Physiol, 1990, 258: [28],,,.

[6] Ellis C S, WatanabeW O, Ellis E P. Temperature effects on feed utilization and growth of postsettlement stage Nassau groper[j]. Trans Amer Fish Soc, 1997, 126: 309 315. [7],,,. 鯻 [J].

7 [J]., 2008, 21(2): [29] MockA, PetersG. Lysozyme activity in rainbow trout Omorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution[j]. J Fish Bio, 1990, 37: [30] Cronin M A, Culloty S C, Mulcahy M F. Lysozyme activity and protein concentraction in the haemolymph of the flat Oyster Ostrea edulis[j]. Fish Shellf Immunol, 2001, 11: [31] Roed K H, Larsen H J, Linder R D,et al.genetic variation in lysozyme activity in rainbow trout ( Oncorhynchus mykiss) [J]. Aquaculture, 1993, 109: [32] Mock A, Peters G.Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution[j]. J Fish Biol, 1990, 37: [33] Yin Z, Lam T J, Sin YM. The effects of crowding stress on the non specific immune response in fancy carp (Cyprinus carpio L)[J]. Fish Shellf Immunol, 1995, 5: [34] Roche H, Boge G. Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication[j]. Mar Envir Res, 1996, 41: [35] Cataldi E, Di Marco P, MandichA, Cataudella,S.Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipenseriformes):effects of temperature and stress[j].comp Biochem Physiol, 1998, 121: [36] Chen G R, Sun LT, Lee Y H, et al. Characteristics of blood in common carp, Cyprinus carpio, exposed to low temperatures[j]. J Appl Aqu, 1995, 5(3): [37] Carine Luisa Lermen, Rosiele Lappe, Marcia Crestani,et al.effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen[j]. Aquaculture, 2004, 239: Effects of water temperature on growth and blood biochemical immunological indices of Scortum barcoo ZHANG Na, LUO Guozhi, TAN Hongxin, SUN Dachuan, PAN Yunfeng, ZHENG Haisu (College of Fisheries and Life Science, Shanghai Ocean University, Shanghai , China) Abstract: The Scortum barcoo was cultured at water temperatures of 16, 22 and 28 in recirculating aquaculture system for four weeks. Its body weight was (107.52±0.87) g.the results showed that the Scortum barcoo cultured at different temperatures expressed different growth traits. The specific growth rate (SGR) at 22 and 28 was significantly higher than that at 16, and SGR at 22 and 28 had no evident difference, and daily weight gain (DWG), specific growth rate (SGR), food conversion ratio (FCR) and feed ration (FR) were influenced significantly by water temperature. In this experiment, the influences of water temperature on blood biochemical immunological indices varied, ALT activity decreased with rising temperature; triacylglycerol, lysozyme and cortisol levels increased along with ascending temperature, whereas spleen weight index went up at beginning and then went down. Statistic analysis revealed that water temperature had no significant effects on ALT activity, triacylglycerol level, lysozyme and spleen weight index. However, cortisol levels were distinctly influenced by water temperature. Taken together, these results suggest that the optimum growth temperature is for S. barcoo. Water temperature at 28 may have chronic stress on S. barcoo, which causing blood cortisol level increasing. [Journal of Fishery Sciences of China, 2010, 17(6): ] Key words: Scortum barcoo; water temperature; growth; blood; biochemical immunological indices Corresponding author: Sun Dachuan. [email protected]

Fish Shellf Immunol, 2001, 11: 611 622. [31] Roed K H, Larsen H J, Linder R D,et al.genetic variation in lysozyme activity in rainbow trout ( Oncorhynchus mykiss) [J]. Aquaculture, 1993, 109: 237 244.

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