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1 36 6 Vol.36, No JOURNAL OF FISHERIES OF CHINA June, 2012 文章编号 : (2012) DOI: /SP.J 鲤脑垂体和 HCG 诱导雌性日本鳗鲡性腺成熟过程中血清生化成分的变化, *,,, (, ) : HCG,,, (TP) (TG) (TC) (Glu) (Ca) (P)6, (GSI), 84 d,, GSI, %± 1.75%; GSI 2.42 %± 0.18% TP, (P > 0.05) 56 d (P < 0.05): TG, TC, (12.67 ± 2.83) mmol/l (15.54 ± 3.01) mmol/l; TG TC, (6.33 ± 4.14) mmol/l (8.53 ± 3.30) mmol/l Glu, TG GSI (r 0.732, n 6, P < 0.01) Ca GSI(r 0.961, n 6, P < 0.01), P GSI(r 0.775, n 6, P < 0.01) : Ca P 28 d, GSI Ca P :, (Ca, P), Ca P, : ; ; ; ; ; ; S 917 A (Anguilla japonica),,,,,, [1],, [2], [3-5], ),,,,,, 收稿日期 : 修回日期 : 资助项目 : ( ) 通讯作者 :, lp-liu@shou.edu.cn

732, n 6, P < 0.01) Ca GSI(r 0.961, n 6, P < 0.01), P GSI(r 0.775, n 6, P < 0.

2 894 36,, 1 1.1,,, 100, 845~1020 g, 15 d, 28, 18~23, HCG, HCG 0.7% 1.2, ( 0.75 mg/kg BW; HCG 150 IU/kg BW),, ;, 1.3, 6, 6 7, MS-222(0.1%), : 2 ml, 1.5 ml 4 2~4 h,, r/min 15 min, [4], ( BS-200) : (TP) (TG) (TC) (Glu) (Ca) (P) : GSI=[ /( )] 100% 1.4 SPSS 11.5, (One-Way ANOVA), Duncan, P<0.05, GSI Excel, d, GSI, 84d 46.32% ± 1.75%, ;, 84 d 2.45% ± 0.30% 图 1 注射脑垂体和 HCG 对雌性日本鳗鲡 GSI 的影响 Fig. 1 Effects of injection of carp pituitary gland and HCG on GSI in female A. japonica 2.2 血蛋白血脂血糖的变化 TP TG TP, ( 2 3) TG : TG (12.47±0.10) mmol/l; TG 14 d, 42 d (5.04±0.72) mmol/l, 56 d (12.54± 0.08) mmol/l, 70 d (5.57±0.06) mmol/l TC, ; TC ( 4), 56 d (P<0.05): TG, TC, (12.67±2.83) mmol/l (15.54±3.01) mmol/l; TG TC, (6.33±4.14) mmol/l (8.53±3.30) mmol/ L

75%, ;, 84 d 2.45% ± 0.30% 图 1 注射脑垂体和 HCG 对雌性日本鳗鲡 GSI 的影响 Fig. 1 Effects of injection of carp pituitary gland and HCG on GSI in female A. japonica 2.

3 6, : HCG 895 图 2 雌性日本鳗鲡卵巢发育期间血清总蛋白的变化 Fig. 2 Changes of the serum TP during ovarian development 图 3 雌性日本鳗鲡卵巢发育期间血清甘油三酯的变化 Fig. 3 Changes of the serum TG during ovarian development 图 5 雌性日本鳗鲡卵巢发育期间血清葡萄糖的变化 Fig. 5 Changes of the serum Glu during ovarian development, Glu,, ( 5) Glu, 28 d 血钙血磷的变化 Ca P, : Ca 28 d (1.06±0.04) mmol/l, 84 d (6.21± 0.40) mmol/l; P 28 d (1.12 ± 0.14) mmol/l, 84 d (4.24 ± 0.02) mmol/l( 6 7) : Ca, 28 d ; P, 图 4 雌性河鳗卵巢发育期间血清胆固醇的变化 Fig. 4 Changes of the serum TC during ovarian development 图 6 雌性日本鳗鲡卵巢发育期间血清中钙的变化 Fig. 6 Changes of the serum Ca during ovarian development 2.3

5 Changes of the serum Glu during ovarian development, Glu,, ( 5) Glu, 28 d 血钙血磷的变化 Ca P, : Ca 28 d (1.06±0.04) mmol/l, 84 d (6.21± 0.40) mmol/l; P 28 d (1.12 ± 0.

4 GSI, 1, TG, Ca, P GSI 0.732, 0.961, 0.775, (P < 0.01); TP, TC, Glu GSI (P > 0.05) 图 7 Fig. 7 雌性日本鳗鲡卵巢发育期间血清中磷的变化 Changes of the serum P during ovarian development 表 1 实验组血液指标与 GSI 的相关分析 Tab. 1 Correlation coefficient between blood indices and GSI of treatment group index correlation coefficient TP TG ** TC Glu Ca ** P ** : * (P<0.05); ** (P<0.01) Notes: * Correlation is significant at level 0.05; ** Correlation is very significant at level Ⅱ [6],, [7-9], 84 d, HCG,, HCG 84 d,,,, 3.2,, [3-5],, [9],,, [1,9-10],,,, ( ) [11] ( ) ( ),, 3~4 [12], (A. anguilla) Glu, 42 d, 56 d [13] (Lutjanusery thoperus) Glu TG, TP [14] (Lateolabrax japonicus) TG TC 3, Glu,, [15], TP, ; TG TC 24 d, 56 d ; Glu, TP TC TG Glu,,,,,,,,,,, TP, TC, TG

1 Correlation coefficient between blood indices and GSI of treatment group index correlation coefficient TP 0.175 TG 0.732 ** TC 0.251 Glu 0.110 Ca 0.961 ** P 0.775 ** : * (P<0.05); ** (P<0.

5 6, : HCG 897, Glu, TC TG, TC [16],,,, TC, TC ;,,, TC, TC [17], [18], [19],,,,,,, Ca 2+, (vitellogenin, Vg),,, Vg Ca, Ca Vg [20-21] Linares-Casenave [22] (Acipenser transmontanus) Vg,, Vg Ca : Vg,, Vg Ca [23] (A. ruthenus), Vg Ca (R 2 = ), Ca, [24] (Pelteobagrus vachelli),,,, [4] (A. baerii), Ca, Ca P,, Ca P 3.3, 2~5, ( ) ( ),, GSI, [25] Okamura [26] 12~56,,,,,,, (3~4 ), GSI, (TC TG) ; (5~6 ),, Ca P GSI,,,,,, : [1],. [J], 1997, 32(3): [2] Wilhelm F D, Jensen E G, Gilson K, et al. Comparative hematology in marine fish [J]. Comparative Bi- ochemistry and Physiology Part A: Physiology, 1992, 102(2): [3] Johnson L L, Casillas E. The use of plasma parameters to predict ovarian maturation stage in English sole Parophrys vetulus girard [J]. Journal of Experimental Marine Biology and Ecology, 1991, 151(2): [4],,,. [J]., 2007, 14(2): [5] Webb M A H, Feist G W, Foster E P, et al. Potential classification of sex and stage of gonadal maturity of wild white sturgeon using blood plasma indicators[j]. Transactions of the American Fisheries Society, 2002, 131(1):

3, 2~5, ( ) ( ),, GSI, [25] Okamura [26] 12~56,,,,,,, (3~4 ), GSI, (TC TG) ; (5~6 ),, Ca P GSI,,,,,, : [1],. [J], 1997, 32(3): 39 48 [2] Wilhelm F D, Jensen E G, Gilson K, et al.

6 [6], [J], 2001, 21(2): [7], [J]., 2003, 12(1): 6 11 [8],,, [J], 2006, 29(5): [9]. [J], 2007, 29(4): [10],,, [J], 1980, 4(2): [11] [M]. :, 1999 [12] Olivereau M, Olivereau J M. Long-term starvation in the European eel: General effects and responses of pituitary growth hormone-(gh) and somatolactin-(sl) secreting cells [J]. Fish Physiology and Biochemistry, 1997, 17(1): [13] Caruso G, Maricchiolo G, Micale V, et al. Physiol ogical responses to starvation in the European eel (Anguilla anguilla): Effects on haematological, biochemical, nonspecific immune parameters and skin structures[j]. Fish Physiology and Biochemistry, 2010, 36(1): [14],, [J], 2004, 25(5): [15], [J], 2002, 9(2): [16], [M] :, 1996 [17],,,. [J]., 2009, 33(6): [18],,, [J] :, 2001, 40(2): [19], [J], 1989, 13(4): [20], [J], 1993, 17(4): [21] Mugiya Y, Watabe N. Studies on fish scale formation and resorption ii. Effect of estradiol on calcium homeostasis and skeletal tissue resorption in the goldfish, Carassius auratus, and the killifish, Fundulus heteroclitus [J]. Comparative Biochemistry and Physiology, Part A: Molecular Integrative Physiology, 1977, 57(2): [22] Linares-Casenave J, Kroll K J, van Eenennaam J P, et al. Effect of ovarian stage on plasma vitellogenin and calcium in cultured white sturgeon [J]. Aquaculture, 2003, 221(1 4): [23],,, Ca 2+ [J], 2009, 16(6): [24], [J], 2004, 23(10): 4 [25] Kagawa H. Estimation of gonadal status of Japanese female eel Anguilla japonica by a single injection of salmon pituitary extract [J]. Fisheries Science, 2005, 71(5): [26] Okamura A, Oka H P, Horie N, et al. Assessing sexual maturity of feminized Japanese eel Anguilla japonica by measuring eye size [J]. Aquaculture International, 2009, 17(1):

Fish Physiology and Biochemistry, 1997, 17(1): 261 269. [13] Caruso G, Maricchiolo G, Micale V, et al.

7 6, : HCG 899 The changes of serum biochemical components during carp pituitary extract and HCG induced maturation of the female Japanese eel (Anguilla japonica) JIANG Tian-bao, LIU Li-ping *, GAO Xiao-yang, CHEN Wen-yin, WU Jia-min (Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai , China) Abstract: Based on the gonadosomatic indexes (GSI) of different developmental ovary stages of the female Japanese eel during carp pituitary extract and HCG induced maturation, the paper measured and analyzed six biochemical components of the serum, namely total protein (TP), triglycerides (TG), total cholesterol (TC), blood glucose (Glu), calcium (Ca) and phosphorus(p) by medical inspection methods. The results showed that the gonad of treatment group got matured gradually during 84 days with GSI increasing significantly to 46.32%±1.75% at the 12th injection; while GSI of the control group stayed at 2.45% ± 0.30%. There was no difference between the TP level of treatment group and control group (P > 0.05) which remained relatively stable. However, the blood lipid displayed significant difference (P < 0.05) until 56th day: TG and TC of the treatment group maintained a high level, averaged at (12.67 ± 2.83) mmol/l and (15.54 ± 3.01) mmol/l respectively; while control group kept a low level, averaged at (6.33 ± 4.14) mmol/l and (8.53 ± 3.30) mmol/l respectively. The Glu level of both groups carried on rising from a low level at the beginning. Correlation analysis indicated that there was significant negative correlations between TG and GSI in treatment group (r = 0.732, n=6, P < 0.01). Significant correlations were found between Ca and GSI (r =0.961, n=6, P < 0.01), P and GSI (r =0.775, n=6, P < 0.01)in treatment group: as the growth rates of GSI, and the level of Ca and P increased dramatically from 28th day, while the control group stayed at a low level. It turned out that there is a close relationship between ovary development and lipid metabolism, and some inorganic ions (Ca, P) have played an important role in this process. According to the nutrition requirement and metabolic rule of the female Japanese eel, it s a new potential way to judge ovary developmental stages by measuring and analyzing the level of blood lipid or serum Ca and P. Key words: Anguilla japonica; gonadosomatic indexes(gsi); serum; triglycerides(tg); total cholesterol(tc); Ca; P Corresponding author: LIU Li-ping. lp-liu@shou.edu.cn

Based on the gonadosomatic indexes (GSI) of different developmental ovary stages of the female Japanese eel during carp pituitary extract and HCG induced maturation, the paper measured and analyzed

第二部分

第二部分中国农通报 0,7(04):478-48 Chinese Agricultural Science Bulletin 基于网络平台的生物统计改革及实践效果分析袁文, 彭惠茹, 张洪亮, 刘文欣, 华金平, 苏胜宝, 孔繁玲 ( 中国农大科技术发展研究院, 北京 0093; 中国农大农与生物