296 FINE CHEMICALS 33 Sinensis Lam extracts = system systemⅢ performed best. Key words natural surfacetant Camellia Oleifera Abel meal Sap

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1 FINE CHEMICALS Vol. 3 3 No. 3 Mar Camellia Oleifera Abel meal Sapindus Mukorossi Gaertn Gleditsia Sinensis Lam Sanherb Rosen m m = Ⅲ 76% m m = Ⅱ % m m = Ⅲ TQ423 A Extraction and Purification of Nonionic Surfactant from Natural Plant and the Evaluation of Their Complex Synergism YU Li-jia YANG Jiang-yong LIU Chang-cheng CHEN Li CHEN Gang HONG Han-jun Sanherb Biotech Co. Ltd. Chengdu Sichuan China Abstract The method of extraction and purification of 3 nonionic surfactants from Camellia Oleifera Abel meal Sapindus Mukorossi Gaertn and Gleditsia Sinensis Lam were researched respectively in addition the influencing factors of their surfactant activity and their complex synergism were evaluated as well. The experimental results show that after extraction and purification the surfactant activity of the final extracts were obviously superior to the original natural materials. The influence factors on surfactant activity also showed that their surfactivity were enhanced while in acidic conditions high temperature and binary mixed systems but the effects of salinity and the hardness of water on their surfactivity were not obvious. Results showed that while the extracts were compounded in the proportion which calculated and conclued based on Rosen theory ability of m Sapindus Mukorossi Gaertn extracts m Gleditsia Sinensis Lam extracts = system systemⅢ to form micelles increaed by 76% than the single component while the ability of m Camellia Oleifera Abel meal extracts m Gleditsia Sinensis Lam extracts = system system Ⅱ to reduce the surface tension enhanced by 10. 5% which indicated both of them have synergistic effect. Furthermore results of foam property testing indicated that compared with the single component the binary mixed systems showed excellent foam property especially the m Sapindus Mukorossi Gaertn extracts m Gleditsia DOI /j. jxhg E - mail tech@ sanherb. com

2 296 FINE CHEMICALS 33 Sinensis Lam extracts = system systemⅢ performed best. Key words natural surfacetant Camellia Oleifera Abel meal Sapindus Mukorossi Gaertn Gleditsia Sinensis Lam extraction and purification surface activity Sapindus Mukorossi Gaertn 2152 Camellia Oleifera Abel Gleditsia Sinensis Ross-Miles LC Lam - 20A g E 2 5 L V V 3 = h 1 L D101 LAS 2 h 1 BV /h AES TX V V FAS ALSA = BV /h TLC ADS h 1 BV /h 2 BV 100% 1 BV /h 40% HPLC HPLC 85% g 5 L 1 V V = AR 1 h 1 L Al 2 SO D101 HP20 1 L ADS - 7 D101 2 h Sephadex LH - 12 Amersham 1 BV /h ~ 300 V V = ZDHW 1 BV /h V V BC-R205 = BZY 52% HPLC PHS - 3C HPLC PH UPR-II-2OL 85%

3 g Table 2 Comparison of each botanical surfactant before and 5 L V V after purification = CMC / γ 3 h 2 h 1 h CMC / /mm CMC / γ CMC / /mm 1 L HP20 V V = V V = % HPLC 1 BV /h g /L 1 HPLC 80% 1. 3 GB /T GB /T Ross-Miles 0. 5 ~ 3. 5 g /L 40 ± 2 Rose-Miles s - 2 g/l mn /m 30 s 180 s 300 s g /100 g HPLC 80% g/l mn /m 30 s 180 s 300 s a b 1 Table 1 Physical index of each botanical surfactant / g /100 g ph /% > > > /% 2. 2 c 1 3 γ CMC CMC 2. 5 g /L Fig. 1 Effects of temperature on the surface tension of the 3 Ross-Miles 2 botanical surfactant with different concentration 2 γ 0 ~ 60

4 298 FINE CHEMICALS ph =3 2b Sapindoside B g / L NaCl 3 a a b b c 2 3 ph Fig. 2 Effects of ph on the surface tension of the 3 botanical surfactant with different concentration c Fig. 3 Effects of NaCl on the surface tension of the 3 botanical ph =3. 63 ~ ph surfactant with different concentration

5 g /L CaCl g MgSO g 1 L m Ca 2 + m Mg mg /L a b 10 - Rosen 11 u = u mg /L u = u = u 4 m 1 m 2 CMC 1 CMC 2 9 m = m = m m = g 100 ml 50 g /L / Ⅰ / Ⅱ / Ⅲ Ⅰ m m = g g 5 g 100 ml Ⅰ 50 g /L Fig. 4 c 3 Effects of hardness of water on the surface tension of the 3 botanical surfactant with different concentration

6 300 FINE CHEMICALS Rosen CMC 1 CMC 2 γ 1 γ 2 = x 1 CMC 1 + x 2 CMC 2 a b c = x 1 γ 1 + x 2 γ 2 system Ⅰ / Ⅰ system x 1 x 2 Ⅱ / Ⅱ system Ⅲ / Ⅲ Fig. 5 Effects of compoud on the surface tension of the 3 botanical A γ CMC surfactant with different concentration 25 B A /% = 1 - / 100 B /% = 1 - γ CMC /γ CMC Table 4 The comparasion of the surface activity synergy of the 3 botanical surfactant Ⅰ Ⅱ Ⅲ / / / γ CMC / mn /m = x 1 CMC 1 + x 2 CMC a b c system Ⅰ / Ⅰ system Ⅱ / Ⅱ system Ⅲ / Ⅲ = x 1 γ 1 + x 2 γ 2 - / g/l / mn /m Fig. 6 Foamming ability of compounded system A = 1 - / 100% B = 1 - / 100% / Sanherb Ⅲ Rosen A Ⅰ Ⅱ B

7 % 1 Yukiyoshi T Kenji M Takao M et al. Antimicrobial activities of Ⅲ > saponins of periearps of Sapindus mukurossi on dermatophytes J. Ⅱ > Ⅰ Natural Medicines Ⅱ > Ⅰ > Ⅲ Ⅲ 2 Wong W H Ryoji K Wakako C et al. Acyclic sesquiterpene 76% Ⅱ oligoglycosides from pericarps of Sapindus delavayi J % Phytochemistry CMC 6 γ CMC ~ 60 Miles S Rosen 10 Rosen M J / Ⅰ / Ⅱ / Ⅲ / J Ⅲ 76% / Ⅱ J J B P. CN A J GB /T S. 8 GB /T Ross- M. Xi Y H. Surface concentration and molecular interactions in binary mixtures of surfactants J. Jounral of Colloid and Interface Science Rosen M J. Surfactants and interfacial phenomena M. New York A John Wily and Sons Inc Publication J EI Compendex EI Compendex EI Compendex EI Compendex EI The Engineering Index EI 1884 Engineering information Inc. EI

H 2 SO ml ml 1. 0 ml C 4. 0 ml - 30 min 490 nm 0 ~ 100 μg /ml Zhao = VρN 100% 1 m V ml ρ g

H 2 SO ml ml 1. 0 ml C 4. 0 ml - 30 min 490 nm 0 ~ 100 μg /ml Zhao = VρN 100% 1 m V ml ρ g 16 6 2018 11 Chinese Journal of Bioprocess Engineering Vol. 16 No. 6 Nov. 2018 doi 10. 3969 /j. issn. 1672-3678. 2018. 06. 004 1 2 1 1 1. 330200 2. 330006 4 Box-Behnken 1 25 g /ml 121 92 min 2 6. 284%