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1 36 3 Vol. 36, No ACTA HYDROBIOLOGICA SINICA May, DOI: /SP.J 杨伟叶继丹王琨孙云章张春晓翟少伟宋凯 (,, ) GLUCOSE TOLERANCE IN GROUPER (EPINPHELUS COIOIDES) YANG Wei, YE Ji-Dan, WANG Kun, SUN Yun-Zhang, ZHANG Chun-Xiao, ZHAI Shao-Wei and SONG Kai (Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College of Jimei University, Xiamen , China) : ; ; ; ; Key words: Epinphelus coioides; Glucose; Insulin; Tolerance test; Plasma metabolites 中图分类号 : S 文献标识码 : A 文章编号 : (2012) ,, [1], [2] [3] [4], :,,,,, [5, 6], : (Carassius auratus gibelio) [7], (Cyprinus carpio) [8], [5],, [9 12] [13], [14] ;,, [15], [5], [16], [17], Shiau Lin [18] (Epinephelus malabaricus),,, (Epinephelus coioides), 1 1.1,,, 4 FRP (900 L), ( 54.4% 10.0%),, 0.5h 1, 18 (120 L), 10 2, 2 ( 收稿日期 : ; 修订日期 : 基金项目 : (3502Z ); ( ); (2011A001) 作者简介 : (1986 ),, ; ; weiyangwfxy428@163.com 通讯作者 : (1966 ),, ; ; yjdwk@sina.com

2 ), (80.5±15.4) g 1.2, 1670 mg/kg (H ), 335 mg/kg (L ), (125 mg L ),,, 1 ml 5 cm,,,, 120min 9 0h h,,, 7% 5 ml, 4000 r/min 10min, 9, 3, 80,, 3, 1.4, (UniCel DxI 800 ),,, 1.5 ± (Mean±SD), SPSS,, Duncan P < Fig. 1 The change of plasma glucose in the grouper over time after oral administration of two doses of glucose (P <0.05); Values with different superscripts in each group indicate significant difference (P <0.05); the same as bellow 2.2 2, 1h, 2.56 pmol/l 0.94 pmol/l, 12h H (1.98 pmol/l), (P> 0.05) 15 18h H, 24h, (P >0.05) L 3 24h, (P <0.05) h 1h, 3h,, 6h, H L, 3h, (P<0.05)( 1) 2 Fig. 2 The change of plasma insulin in the grouper over time after oral administration of two doses of glucose

3 3 : , H (100.5 mg/g), (P >0.05) L, 3h 12h ( 55.4 mg/g 61.2 mg/g), 15h, 24h (P >0.05) 2.4,,, 24h ( 4) H L 9h 3h, 15h 9h, h, ( 5), 15h 1 15h, H L, L, 18 24h H (P <0.05), L, 24h (P >0.05) 3 Fig. 3 The change of liver glycogen in the grouper over time after oral administration of two doses of glucose 4 Fig. 4 The change of plasma lactose in the grouper over time after oral administration of two doses of glucose 5 Fig. 5 The change of plasma triglyceride in the grouper over time after oral administration of two doses of glucose 3,, 3h,, [9, 11, 12, 15, 19], :, 5h [15], (Ctenopharyngodon idellus) 10h [12], (Mylopharyngodon piceus) (420 mg/kg ) 10h, (1370 mg/kg ) 10h [12] (1000mg/kg ), (Scophthalmus maximus) 24h [20], (Sparus aurata)(dicentrarchus labrax) 12h [9] (Oncorhynchus mykiss) 3000 mg/kg, 18h [14]

4 h,,,, [5],,,,,,,,,, [2],, [6, 21], Thorpe Ince [22] (Gadous macrocephaius) (Anguilla anguilla), Thorpe Ince [22] (Esox lucius),,, Mazur [23] (Oncorhynchus tshawytscha), Gutierrez, et al. [25, 26] (Dicentrarchus labrax),, Ronner Scarpa [27] (Ictalurus punctatus), Sheridan, et al. [28],,, :, [15, 24], [29], [23], Legate, et al. [10] [30] (Silurus meridionalis),,,,,,, [11] 3,, 2h, [12],, (1370 mg/kg ) 1h 3h, ; (420 mg/kg ) 3h, 3h,, (1000 mg/kg ), 1h, 3h [9] Peres, et al. [9], [11] [12] Peres, et al. [9],,,,,,,, 3h 12h, 3h, 12h,,,,, [31] 1h,, [19],,,,,, [32], 0 15h,,, (1370 mg/kg )(420 mg/kg ) 3h, [12] (420 mg/kg ) 3h,, (1370 mg/kg ) [12] 0 1h, 1 4h [19] Harmon Sheridan [33],

5 3 : 567 : [1] Cai C F, Chen L Q. Comment on the utilization of dietary carbohydrates in fish [J]. Acta Hydrobiologica Sinica, 2006, 30(5): [,.., 2006, 30(5): ] [2] Wilson R P, Poe W E. Apparent inability of channel catfish to utilize dietary mono and disaccharides as energy source [J]. Journal of Nutrition, 1987, 117(2): [3] Dixon D G, Hilton J W. Influence of available dietary carbohydrate content on tolerance of waterborne copper by rainbow trout, Salmo gairdneri Richardson [J]. Journal of Fish Biology, 1981, 19(5): [4] López-Olmeda J F, Egea-Álvarez M, Sánchez-Vázquez F J. Glucose tolerance in fish: Is the daily feeding time important [J]? Physiology & Behavior, 2009, 96(2 3): [5] Moon T W. Glucose intolerance in teleost fish: fact or fiction [J]? Comparative Biochemistry and Physiology, 2001, 129(B): [6] Mommsen T P, Plisetskaya E M. Insulin in fishes and agnathans-history, structure, and metabolic regulation [J]. Reviews in Aquatic Sciences, 1991, 4: [7] Cai C F, Wang D Z. Utilization of carbohydrate in allogynogenetio crucian carp A test of sensitivity to exogenous insulin [J]. Journal of Fishery Sciences of China, 1999, 6(1): [,. -., 1999, 6(1): 62 65] [8] Hertz Y, Epstein N, Abraham M, et al. Effects of metformin on plasma insulin, glucose metabolism, and protein synthesis in the common carp (Cyprinus carpio L.) [J]. Aquaculture, 1989, 80(1 2): [9] Peres H, Goncalves P, Oliva-Teles A. Glucose tolerance in gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) [J]. Aquaculture, 1999, 179(1 4): [10] Legate N J, Bonen A, Moon T W. Glucose tolerance and peripheral glucose utilization in rainbow trout (Oncorhynchus mykiss), american eel (Anguilla rostrata), and black bullhead catfish (Ameiurus melas) [J]. General and Comparative Endocrinology, 2001, 122: [11] Cai C F, Wang D Z. Study on the glucose tolerance of allogynogenetic crucian carp [J]. Journal of Shanghai Fisheries University, 1998, 7(suppl.): [,. [J]., 1998, 7 (): 63 66] [12] Huang H Z, Ding L, Song X H, et al. Comparative research on glucose tolerance between black carp Mylopharyngodon piceus and grass carp Ctenopharyngodon idellus [J]. Journal of Fishery Sciences of China, 2005, 12(4): [,,,.., 2005, 12(4): ] [13] Hilton J W, Plisetskaya E M, Leatherland J F. Does oral 3, 5, 3 -triiodo-l-thyronine affect dietary glucose utilization and plasma insulin levels in rainbow trout (Salmo gairdneri) [J]? Fish Physiology and Biochemistry, 1987, 4(3): [14] Harmon J S, Eilertson C D, Sheridan M A, et al. Insulin suppression is associated with hypersomatostatinemia and hyperglucagonemia in glucose-injected rainbow trout [J]. American Journal of Physiology, 1991, 261(3): [15] Furuichi M, Yone Y. Change of blood sugar and plasma insulin levels of fishes in glucose tolerance test [J]. Bulletin of the Japanese Society of Scientific Fisheries, 1981, 47: [16] Miao S, Tang H C. Bioeconomic analysis of improving management productivity regarding grouper (Epinephelus malabaricus) fanning in Taiwan [J]. Aquaculture, 2002, 211: [17] Millamena O M. Replacement of fish meal by animal by-product meals in a practical diet for grow-out culture of grouper Epinephelus coioides [J]. Aquaculture, 2002, 204 (1 2): [18] Shiau S Y, Lin Y H. Carbohydrate utilization and its protein-sparing effect in diets for grouper (Epinephelus malabaricus) [J]. Animal Science, 2001, 73: [19] Cai C F, Liu Y, Chen L Q, et al. Metabolic responses of allogynogeneticgbel carp after oral administration of different doses of glucose [J]. Acta Hydrobiologica Sinica, 2003, 27(6): [,,,.., 2003, 27(6): ] [20] Garcia-Riera M P, Hemre G.-I. Glucose tolerance in turbot, Scophthalmus maximus (L.) [J]. Aquaculture Nutrition, 1996, 2(2): [21] Gutierrez J, Asgard T, Fabbri E, et al. Insulin-receptor binding in skeletal muscle of trout [J]. Fish Physiology and Biochemistry, 1991, 9(4): [22] Thorpe A, Ince B W. Plasma insulin levels in teleosts determined by a charcoal-separation radioimmunoassay technique [J]. General and Comparative Endocrinology, 1976, 30(3): [23] Mazur C N, Higgs D A, Plisetskaya E, et al. Utilization of dietary starch and glucose tolerance in juvenile chinook salmon (Oncorhynchus tshawytscha) of different strains in seawater [J]. Fish Physiology and Biochemistry, 1992, 10(4): [24] Enes P, Peres H, Pousão-Ferreira P, et al. Glycemic and insulin responses in white sea bream Diplodus sargus, after

6 intraperitoneal administration of glucose [J]. Fish Physiology and Biochemistry, 2011, /s [25] Gutierrez J, Carrillo M, Zanuy S, et al. Daily rhythms of insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding [J]. General and Comparative Endocrinology, 1984, 55(3): [26] Gutierrez J, Fernandez J, Carrillo M, et al. Annual cycle of plasma insulin and glucose of sea bass. Dicentrarchus labrax, L [J]. Fish Physiology and Biochemistry, 1987, 4(3): [27] Ronner P, Scarpa A. Difference in glucose dependency of insulin and somatostatin release [J]. American Journal of Physiology - Endocrinology and Metabolism, 1984, 246(6): [28] Sheridan M A, Eilertson C D, Plisetskaya E M. Radioimmunoassay for salmon pancreatic somatostatin-25 [J]. General and Comparative Endocrinology, 1991, 81(3): [29] Enes P, Peres H, Sanchez-Gurmaches J, et al. Insulin and IGF-I response to a glucose load in European sea bass (Dicentrarchus labrax) juveniles [J]. Aquaculture, 2011, 315(3 4): [30] Fu S J, Xie X J. Effect of dietary carbohydrate levels on growth performance in Silurus meridionalis Chen [J]. Acta Hydrobiologica Sinica, 2005, 29(4): [,.., 2005, 29(4): ] [31] Kumar V, Sahu N P, Kumar S, et al. Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet [J]. Fish Physiology and Biochemistry, 2010, 36(3): [32] Suarez M D. Metabolic effects of changes in the dietary protein: carbohydrate ratio in eel (Angilla anguilla) and trout (Oncorhynchus mykiss) [J]. Aquaculture International, 2002, 10(2): [33] Harmon J S, Sheridan M A. Glucose-stimulated lipolysis in rainbow trout, Oncorhynchus mykiss, liver [J]. Fish Physiology and Biochemistry, 1992, 10(3):

60 6 harmful to the growth and metabolism of the fish. Key words: Jade perch ( Scortum barcoo) ; digestible carbohydrate; blood biochemical indices; g

60 6 harmful to the growth and metabolism of the fish. Key words: Jade perch ( Scortum barcoo) ; digestible carbohydrate; blood biochemical indices; g 6 2 2 0 1 0 4 South China Fisheries Science Vol. 6, No. 2 Apr., 2010 doi: 10. 3969/ j. issn. 1673-2227. 2010. 02. 010 1, 2, 3 2 1 1 1,,,, ( 1.,, 300384; 2., 100068; 3., 510300) : 0% 6%23%40% ( Scortum

Microsoft Word - 24 杨 伟.doc

Microsoft Word - 24 杨伟.doc