Microsoft Word 陈笑笑-二校出4815

Size: px

Start display at page:

Download "Microsoft Word 陈笑笑-二校出4815"

墀查
5 years ago
Views:

1 7 12 Vol. 7 No Journal of Food Safety and Quality Dec., 2016 陈笑笑 1, 赵芸 2, 柳爱春 2, 陈飞东 2, 胡叶军 1*, 叶聪莹 (1., ; 2., ) 1 摘要 :,,,,,,,,, 关键词 : ; ; ; Research progressof detection methods for streptomycin residue in food CHEN Xiao-Xiao 1, ZHAO Yun 2, LIU Ai-Chun 2, CHEN Fei-Dong 2, HU Ye-Jun 1*, YE Cong-Ying 1 (1. Hangzhou Nankai Biotech Co., Ltd., Hangzhou , China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou , China) ABSTRACT: Streptomycin is a kind of broad-spectrum aminoglycoside antibiotics. Because of its good antibacterial effect and low price, it is often used as a feed additive, treatment agent, fruit and vegetable disease control agent which are usually used in animal husbandry, apiculture, aquaculture and agriculture. The streptomycin residue in animals and plants increases by the extensive use of streptomycin in agriculture and animal husbandry. Then the streptomycin residue enters human body by the food chain and causes great harm to human health. Many countries have paid great attention to streptomycin residue in food, and set up streptomycin residue limit standards. Considering food safety, it is of great significance to establish a method for the detection of streptomycin in food. This paper provided a comprehensive overview of the research status of streptomycin detection technologies in food, and focused on the microbiological assay, immunoassay, chromatography, and spectrophotometric method for the detection of streptomycin. The advantages and disadvantages of these detection methods were also analyzed as well as the development trend. KEY WORDS: streptomycin; residue; food; detection 1 引言 (streptomycin, STR) 1944 Selman Abraham Waksman,, [1], 基金项目 : 2013 ( A05) Fund: Supported by the Hangzhou Major Scientific and Technological Innovation Project in 2013 ( A05) * 通讯作者 :,, hyj664@163.com *Corresponding author: HU Ye-Jun, Assistant Engineer, Hangzhou Nankai Biotech Co., Ltd., Hangzhou , China. hyj664@ 163.com

2 4816 7, [2], [3],, [3], [4],,, [5] ;, [6] ;, [7], [8] ;, [9] (EC) No 470/2009, 1000 μg/kg, 200 μg/kg, 500 μg/kg [10], 2000 μg/kg, 500 μg/kg [11] 2002, 200 μg/kg, 600 μg/kg, 1000 μg/kg [12],,,, 2 微生物法, [13] Swab Test on Premises(STOP) [14] Fast Antibiotic Test(FAST) [15] TTC [16] [17] [18],, 0.1 μg/ml, 200 μg/kg [19] TTC,, 660 μg/kg,, [20], 80 μg/kg,,,,,, [19,21,22], 3 免疫分析法, 3.1 酶联免疫分析法 (enzyme-linked immunosorbent assay, ELISA),, [23] [24] ELISA 2.7 ng/ml 2.8 ng/ml, [25] ELISA, 4 μg/kg, 0.1 μg/kg, [25] ELISA, 0.5~128 μg/l, 30 μg/kg ELISA [26],, 3.2 放射免疫测定法 (radioimmunoassay, RIA) [27], [28] Charm Ⅱ, 25~500 μg/l,, 200 μg/kg [29] Charm Ⅱ, 10 μg/kg,,,, [29], [30], 3.3 胶体金免疫层析技术 (gold immunochromatography assay, GICA) 3 [31] [32], 10 ng/ml, 40 ng/ml [33],, 8 μg/l, 100 μg/l,,,,, [32-34],,

3 12, : 色谱分析法,,, [35] 液相色谱法,, (high performance liquid chromatography, HPLC),,, [36] [37] - [36] [37], -, mg/kg [38],, 10 μg/kg,,,, [36],,,, 4.2 色谱 - 质谱联用法 (hydrophilic interaction chromatography, HILIC), 玥 [39],, 0.5 μg/kg 1 μg/kg [40],, - (UPLC-MS/MS), 10 μg/l 5 化学发光法 (chemiluminescence, CL),, [41] [42] 100 g/l, C 18, ph, 50 μg/kg,, [43], 6 分光光度法, [44], (o-phthalaldehyde, OPA),,, -NH 2, 1,2- -4-,, [45],,,,, [46], 7 共振瑞利散射法 (resonance Rayleigh scattering, RRS),, [47,48], [49] Triton X-100,, RRS,,,, [50] 8 存在的问题与发展前景,, -,,,,,

4 参考文献 [1]. [J]., 1998, 24(3): Sheng XM. Chinese veterinary drug knowledge [J]. Chin J Vet Med, 1998, 24(3): [2] Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. Aminoglycosides: activity and resistance [J]. Antimicrob Agents Chemother, 1999, 43(4): [3],,,. [J]., 2015, (4): Chen X, Qu SC, Huang DL, et al. Research progress on residues and metabolism of streptomycin in animals [J]. J Inspect Quarant, 2015, (4): [4] Unusan N. Occurrence of chloramphenicol, streptomycin and tetracycline residues in ultra- heat- treatment milk marketed in Turkey [J]. Inter J Food Sci Nut, 2009, 60(5): [5]. [J]. :, 1989(1): Hu CQ. Streptomycin immunology [J]. World Notes Antibiot, 1989, (1): [6]. [J]., 2003, 15(1): Zhao DY. Contamination and control of hormone and antibiotic residues in foods [J]. Chin J Food Hyg, 2003, 15(1): [7] 洤,. [J]., 1963, (2): Zang YQ, Qin TQ. Neurotoxicity of streptomycin [J]. Acta Anat Sin, 1963, (2): [8],,,. [J]., 2012, 10(2): Wu Y, Zheng J, Zheng BJ, et al. Protection and modification of aminoglycoside antibiotic on ototoxicity [J]. Chin J Otol, 2012, 10(2): [9] Wu JX, Zhang SE, Zhou X. Monoclonal antibody-based ELISA and colloidal gold-based immunochromto -graphic assay for streptomycin residue detection in milk and swine urine [J]. J Zhejiang Univ Sci B, 2010, 11(1): [10] (EC) No 470/2009 Laying down Community procedures for the establishment of residue limits of pharmacologically active substances in foodstuffs of animal [S]. [11] Health Canada, List of maximum residue limits (MRLs) for veterinary drugs in foods [EB/OL]. http: //www. hc-sc. gc. ca/dhp-mps/vet/mrl-lmr/mrl-lmr_versus_ new-nouveau-eng. php, [12]. ( )[J]., 2003, 25(20): Ministry of agriculture animal husbandry and veterinary science. Ministry of agriculture issued the maximum residue limits of veterinary drug residues in animal foods [J]. Chin J Vet Drug, 2003, 25(20): [13],,,. [J]., 2007, 39(4): Song WW, Chai CY, Liu GY, et al. Research progress on detection methods of chloramphenicol residues in animal foods [J]. Anim Husb Vet Med, 2007, 39(4): [14] Dey BP, Alice T, Frank G. Analysis of microbiological screen test data for antimicrobial residues in food animals [J]. J Env Sci Heal Part B, 2003, 38(3): [15] Dey BP, Thaker NH, Bright SA, et al. Fast antimicrobial screen test (FAST): improved screen test for detecting antimicrobial residues in meat tissue [J]. J AOAC Int, 2005, 88(2): [16] Mathews KS, Mclaughlin DP, Ziabari LH, et al. Rapid quantification of ischaemic injury and cerebro -protection in brain slices using densitometric assessment of 2,3,5-triphenyltetrazolium chloride staining [J]. J Neurosci Methods, 2000, 102(1): [17],,,. FilmplateTM [J]., 2015, 33(1): Ma QX, Liu N, Cong LM, et al. Comparison between modified filmplate TM method and national standard method for the detection of coliform groups [J]. J Prev Med Chin PLA, 2015, 33(1): [18],,. [J]., 2004, 38(11): 7 9. Cai JH, Liu YN, Gu X. Determination of streptomycin residues in milk by microbiologic inhibition tests [J]. Chin J Vet Drug, 2004, 38(11): 7 9. [19],,. TTC [J]., 2006, (9): Huang YJ, Jiang YW, Hu SH. Detection of antibiotics in milk by TTC [J]. Chin Dairy Cattl, 2006, (9): [20],,. [J]., 2013, (3): Wang CY, Ma K, Wang YJ. Construction of and detection of antibiotic residues in milk powder by microbiological method[j]. Zhejiang Agric Sci, 2013, (3): [21],,,. [J]. :, 2005, 29(1): Song J, Song YQ, Wang YX, et al. Application of microbiological method in the detection of chloramphenicol residues in milk [J]. J Hebei Normal Univ (Nat Sci Ed), 2005, 29(1): [22],,. [J]., 2008, 25(9): Chen X, Zhu LQ, Qi KZ. Research progress on detection of antibiotic residues in animal derived food by microbiological method [J]. Chin J Anim Quarant, 2008, 25 (9): [23],,,. [J]., 2007, (1): Wu ZP, Xu CX, Gao W, et al. Enzyme immunoassay and its application in detection of pesticide residues in food [J]. Jiangsu Agric Sci, 2007, (1): [24],,,. ELISA [J]., 2013, 13(11): Xi Q, Zhang MZ, Li MJ, et al. The establishment of direct ELISA method for streptomycin residue detection[j]. J Chin Inst Food Sci Tech, 2013, 13(11): [25] Hüttenhain R, Malmström J, Picotti P, et al. Perspectives of targeted mass spectrometry for protein biomarker verification[j]. Curr Opin Chem Biol,

5 12, : , 13(5 6): [26],,,. [J]., 2013, 34(16): Guo ZB, Zhao YZ, Wu YX, et al. Competitive indirect ELISA for determination of streptomycin in royal jelly [J]. Food Sci, 2013, 34(16): [27]. [J]., 1983, (Z1): Rui DH. Radioimmunoassay [J]. Heilongjiang Med Pharm, 1983, (Z1): [28],,. 2 [J]., 2003, 24(4): Qin Y, Bao LJ, Zhu LM. The determination of streptomycin residue in chicken liver with two immunoassay methods [J]. J South Chin Agric Univ, 2003, 24(4): [29],,. [J]., 2012, 20(4): Xu HJ, Xie ML, Lin F. The detection method of Streptomycin antibiotic residues in honey-radioimmunoassay [J]. Adv Meas Lab Manag, 2012, 20 (4): [30],,. [J]., 1994, (3): Liu RK, Sun GK, Liu Z. Radioimmunoassay [J]. Chin J Drug Depend, 1994, (3): [31],. [J]., 2005, 33(3): Yan H, Shen HF. Application and prospect of colloidal gold immunochromatography technology [J]. Prog Microbiol Immunol, 2005, 33(3): [32],,,. [J]., 2008, 25(5): He FY, Wan ZP, Ding SY, et al. Research and development of immunochromatographic colloid gold strips for rapid detection of streptomycin [J]. Chin J Anim Quarant, 2008, 25(5): [33],,,. [J]., 2010, 19(2): Fan GY, Chen YY, Wang SG, et al. Development and trait identification of colloid gold-labeled strip for rapid detection of streptomycin [J]. Acta Agric Boreali-occidentalis Sin, 2010, 19(2): [34]. [D]. :, Ni TH. Research and development of immunochromatographic colloid gold strips for rapid detection of streptomycin [D]. Yangzhou: Yangzhou University, [35]. [M]. :, Lin ZK. Chromatography [M]. Beijing: The Science Publishing Company, [36],,,. [J]., 2004, 25(1): Chen XH, Liu XL, Dong MS, et al. Liquid chromatographic detection of streptomycin residues in honey by using post column derivatization [J]. Food Sci, 2004, 25 (1): [37],. - [J]., 2005, 21(1): Jiang L, Zhao SC. Rapid determination of streptomycin residues in milk with post column derivatization liquid chromatography: English edition, [J]. J Mol Sci, 2005, 21(1): [38],,,. [J]., 2008, 19(21): Xue XF, Wu LM, Chen LZ, et al. Study on extraction, purification and chromatographic analysis of streptomycin residue in royal jelly [J]. Food Sci, 2008, 19(21): [39] 玥,,. - [J]., 2009, 34(11): Du Y, Yang HY, Xu WD. Determination of streptomycin and dihydrostreptomycin in honey by hydrophilic interaction chromatography combined with tandem-mass spectrometry [J]. Chin J Antibio, 2009, 34(11): [40],,,. UPLC-MS/MS [J]., 2013, 41(12): Cui XY, Zhang XF, Yuan FQ, et al. Determination of streptomycin and dihydrostreptomycin residues in milk by UPLC-MS/MS [J]. China Dairy Ind, 2013, 41(12): [41],,,. [J]., 2015, (4): Hu XN, Wang R, Xin HF, et al. Study on the application of chemicalluminescence method in the rapid detection of pesticide residues [J]. Qual Saf Agro-Prod, 2015, (4): [42],,,. [J]. :, 2007, 25(3): Wang L, Jiang J, Zhang Y, et al. Determination of streptomycin residues in seafood by luminol-kio 4 -Mn chemiluminescence system [J]. J Guangxi Normal Univ (Nat Sci Ed), 2007, 25(3): [43],,. [J]., 2015, 34(5): Zhao HY, Cai C, Wang YC. Determination of oxytetracycline residue in honey by capillary electrophoresis and electrochemiluminescent detection [J]. J Instrum Anal, 2015, 34(5): [44],. [J]., 2009, (10): Fang L, Guan CM. Determination of streptomycin residues in fresh milk by derivative UV-spectrophotometric method [J]. Chin J Heal Lab Technol, 2009, (10): [45],,,. [J]., 2008, (5): Li YJ, Luo XL, Luo RF, et al. Rapid assay of streptomycin in tomato by fluorescence spectrophotometry [J]. Food Ind, 2008, (5): [46],,,. [J]., 2007, 36(12): Cai Z, Gan BB, Li SG, et al. Application of ultraviolet-visible spectrophotometry in analysis of antibiotics [J]. Tech Dev Chem Ind, 2007, 36(12): [47]. [D]. :, Wang J. Study on resonance rayleigh scattering method for the determination of fluoroquinolones [D]. Chongqing: Southwest University, 2008.

4820 7 [48]. [D]. :, 2009. Liang D. Study on the application of resonance rayleigh scattering and high performance liquid chromatography in pharmaceutical analysis [D].

Chin J Antibio, 2012, 37(1): 59 62. [50],,,. [J]. :, 2008, 30(9): 62 65. Xie LS, Liu S, Sheng Y, et al. Determination of streptomycin with barium chloride by resonance rayleigh scattering method [J].

6 [48]. [D]. :, Liang D. Study on the application of resonance rayleigh scattering and high performance liquid chromatography in pharmaceutical analysis [D]. Chongqing: Southwest University, [49],,. [J]., 2012, 37(1): Pang XD, Huang JQ, Jiang H. Determination of chloramphenicol by resonance rayleigh scattering spectra of alizarin [J]. Chin J Antibio, 2012, 37(1): [50],,,. [J]. :, 2008, 30(9): Xie LS, Liu S, Sheng Y, et al. Determination of streptomycin with barium chloride by resonance rayleigh scattering method [J]. J Southwest Agric Univ (Nat Sci Ed), 2008, 30(9): 作者简介 ( 责任编辑 : 杨翠娜 ) 陈笑笑, 助理工程师, 主要研究方向为食品安全检测 @qq.com 胡叶军, 助理工程师, 主要研究方向为食品安全检测 hyj664@163.com 食品贮藏保鲜与品质控制专题征稿函,,,,,,, (1) ; (2) ; (3) ; (4) / ; (5), ,,, 投稿方式 : : jfoodsq@126.com 食品安全质量检测学报编辑部

Microsoft Word - 3098-3102 20150611007-蔡伟江-二校

Microsoft Word - 3098-3102 20150611007-蔡伟江-二校 6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug., 2015 蔡伟江 *, 邓鹏飞, 刘光兰, 李丹 (, 519040) 摘要 : 目的, 方法 (4.6 mm 250 mm, 5.0 μm) ; : : : =70: 16: 14(V:V:V),, : 1.0 ml/min; : 40 ; : 10

Microsoft Word 陈笑笑-二校 出4815

Microsoft Word 陈笑笑-二校出4815