Microsoft Word 李莎莎-二校出3648 不要动版.doc

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1 5 11 Vol. 5 No Journal of Food Safety and Quality Nov., 2014 李莎莎, 高玎玲, 木其尔, 顾翔宇, 刘莉敏, 郭 * 军 (, ) 摘要 : 目的方法 69, 结果 , 结论,, 关键词 : ; ; ; ; Survey on melamine residue of dairy products sampled from supermarkets in Hohhot city LI Sha-Sha, GAO Ding-Ling, MU Qi-Er, GU Xiang-Yu, LIU Li-Min, GUO Jun * (Food Science and Engineering College of Inner Mongolia Agricultural University, Hohhot , China) ABSTRACT: Objective To investigate the situation of melamine residue in dairy products in Hohhot. Methods A total of 69 milk powder of leisure dairy food and Mongolian conventional cheese and milk-skin samples, as well 128 liquid milk samples of 4 brands were collected from supermarkets of Hohhot city. Melamine residue was test by a high performance thin layer chromatography (HPTLC) method and an immune colloidal gold test kit (ICGT). Results Except 7 milk powder made leisure foods and 2 dairy factory made cheese samples were suspicious (±), the remaining of 197 dairy samples were melamine free ( ). Conclusion The HPTLC methods was not as sensitive as the ICGT, however, it is a cheaper method for daily melamine residues screening. KEY WORDS: melamine; dairy products; liquid milk; immune colloidal gold test; high performance thin layer chromatography 1 引言 2008, MA, [1-4] 10 8,, 1.0 mg/kg, 1.0 mg/kg ; ( ) 2.5 mg/kg, 2.5 mg/kg ; 15% 2.5 mg/kg, 2.5 mg/kg [5-9] 基金项目 : (2012BAD33B01) Fund: Supported by Project of National Key Technology Research and Development Program for The 12th Five-year Plan (2012BAD33B01) 通讯作者 :,,, guojunge@imau.edu.cn *Corresponding author: GUO Jun, Professor, Food Science and Engineering College of Inner Mongolia Agricultural University, Hohhot , China. guojunge@imau.edu.cn

2 11, : 3649,,, [10],, ( ), [11], 2 材料与方法 2.1 样品 ( ); ( ) 2.2 检测方法高效薄层色谱法 (HPTLC), [12], 免疫胶体金 (Immune colloidal gold, ICG),,, 检出限 MA,, MA,, + ; MA,, ; MA,, MA,, ± 0.5 mg/l 0.2 mg/l, 2008 [13] 3 结果与分析 (±), 结论与讨论 MA, MA MA,, 9 MA,,, ; MA MA,, [14,15], 8~12,, MA, 1, -, [16-17] 表 1 HPTLC 和 ICG 两种三聚氰胺检验方法的检出限 ( 单位 : mg/l) Table 1 Melamine test limit of HPTLC and ICG(mg/L) MA (mg/l) ± ± ± : + ; ; ±

3 Table 2 表 2 休闲和传统乳制品抽检样品检测结果 Melamine test results of leisure and traditional dairy food / ± ± ± ± / ± 13 IR/ ± 14 03/ ± 15 kt/ AF02/ / ;08/ AN/ / IBUY / AZD/ IBUY / / / / T/

4 11, : 3651 续表 2 / 34 1C2293/ ± ±

5 表 3 液态奶抽检样品检测结果 Table 3 Melamine test results of liquid milk /

6 11, : 3653 续表 3 /

7 续表 3 /

8 11, : 3655 续表 3 / ( ) (2013) [11], ( ) ( ) 参考文献 [1],,,. [A]. [C]., 2009: Guo J, Zhao L Q, Li CZ, et al. Discuss on Possible Sources and Routes of Melamine Contamination in Animal Derived Foods [A]. Proceedings of The Second International Food Safety Peak BBS [C]. Beijing, 2009: [2],,,. [J]., 2012, 33(7): Sun MQ, Li C Z, Zhang W, et al. The research progress of toxicology melamine [J]. Anim Husb Feed Sci, 2012, 33(7): [3]. [J]., 2008, 16(6): 2. China's food soil livestock import and export chamber of commerce. Melamine storm spread to the vegetables and mushroom [J]. J Zhejiang Edible Mushrooms, 2008, 16(6): 2. [4],,,. [J], 2010, 31(1): Duan WZ, Ma YS, Chen RC, et al. Melamine Contamination and Control Countermeasures in Food [J]. Food Sci, 2010, 31(1): [5],. [J]., 2011: 11. Zhou TY, Hu H. China formulated limited value melamine in food [J]. Beijing Agric, 2011: 11. [6]. [N]., (011). Zhang Y. Five departments jointly issued the melamine limited value in milk products [N].Guangming Daily, (011). [7] [J]., 2011, 11(3): 103. China formally formulated melamine limited value in Food [J]. J

9 Chin Inst Food Sci Technol, 2011, 11(3): 103. [8],. [N]., (001). Liu ZZ, Guan JK. China provides containing melamine limited value in Food [N]. China Food News, (001). [9] [J]., 2011, 4: 64. Five departments formally formulated limit values melamine in Food [J]. Popular Standardization, 2011, 4: 64. [10] (2010 ) (2010 ) [J]., 2010, (4): 7. The State Administration of Quality Supervision, Inspection and Quarantine Notice 2010 No. 119[J]. Guangdong Dairy Production, 2010, (4):7. [11],,,. [J]., 2013, 19(12): 7 9. Bao HQ, Li SS, Guo J, et al. Survey on dextrin adulteration in leisure and traditional dairy food derived from Hohhot city [J]. Chin Food Nutr, 2013, 19(12): 7 9. [12]. [D]. :, Meng LH. Preliminary studies on Melamine Determination by HPTLC in Milk [D]. Hohhot: Inner Mongolia Agricultural University, [13],,,. [J]., 2013, 41(7): Li SQ, Cao BY, Chang HF, et al. Colloidal gold immunochromatographic assay for rapid detection of melamine in milk, milk powder and animal feeds [J]. Chin J Anal Chem, 2013, 41(7): [14] GB/T , [S] GB/T , Determination of melamine in raw milk and dairy products[s] [15],,. [A]. [C].2011, Guo J, Jing Y, Meng LH. Gap and its practical influences exist in dairy product melamine residues determination technology system of our country[a]. Proceedings of The Fourth China International Food Safety Peak BBS on Beijing[C]. 2011: [16],,,. [J]., 2008, 24(10): Feng JW, Yu YG, Ouyang YY, et al. Development of analytical techniques for detection of melamine in food[j]. Mod Food Sci Technol, 2008, 24(10): [17],,. LC- MS. [J]., 2008, (4): Feng W, Wang BC, Mi PC.A novel chrom autographic analysis method of melamine for LC-MS [J]. Guangdong Agric Sci, 2008, (4): [18],,. HPTLC [J]., 2011, 39(6): Meng LH, Liu XK, Guo J. Preliminary establishment of a high sensitivity melamine determination method by HPTLC [J]. China Dairy Ind, 2011, 39(6): 作者简介 ( 责任编辑 : 白洪健 ) 李莎莎, 硕士, 主要研究方向为食品质量与安全 lishasha @163.com 郭军, 博士, 教授, 主要研究方向为营养与食品安全 guojunge@imau.edu.cn

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<4D6963726F736F667420576F7264202D203631372D3632312032303133303430333030312DBACEC0F25FD0A3B6D4B8E55F2DB6FED0A32D2D2DC8A5B5F4CDBCD6D0B5C4BBD8B3B5B7FBBAC52E646F63> 第 4 卷第 2 期食品安全质量检测学报 Vol. 4 No. 2 2013 年 4 月 Journal of Food Safety and Quality Apr., 2013 何莉 *, 姜笑寒 ( 广东省食品药品职业技术学校, 广州 510663) 摘要 : 本文通过科学地运用 html5+jsp+sql 技术, 建

Microsoft Word 李莎莎-二校 出3648 不要动版.doc

Microsoft Word 李莎莎-二校出3648 不要动版.doc