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1 4 1 Vol. 4 No Journal of Food Safety and Quality Feb., 2013 陈永平 *, 张素青, 李春青, 李连庆, 马丹, 高丽娜, 张萍 (, ) 摘要 : 目的方法, HPLC, SPSS 结果 µg/kg, 57.9% 48.0% 23.0%, 46.6% 43.4% 19.8% 50 µg/kg, K (50 µg/kg) = d 1, K (50 µg/kg) = d -1, K( 50 µg/kg )= d 1, K (50 µg/kg) = d 1 K (111.2 µg/kg, )= d 1, K (50 µg/kg, )= d 1 结论 ; ; 关键词 : ; ; ; Studies on degradation kinetics of malachite green and leucomalachite green in different aquatic products CHEN Yong-Ping *, ZHANG Su-Qing, LI Chun-Qing, LI Lian-Qing, MA Dan, GAO Li-Na, ZHANG Ping (Superision and Test Center for Fisheries Environment and Quality of Fishery Products of Ministry Agriculture, Tianjin , China) ABSTRACT: Objective To study degradation kinetics of malachite green and leucomalachite green in different aquatic products. Methods After extracted and purified, samples were detected by high pressure liquid chromatography, and the results were analyzed with SPSS software and degradation kinetics software. Results Degradation of malachite green and leucomalachite green in different aquatic products matrix was fit to pseudo-first order kinetics equations.the degradation rates of malachite green were 57.9%, 48.0%, 23.0%, and the degradation rates of leucomalachite green were 46.6%, 43.4%, 19.8% in three aquatic products matrixes, respectively. For carp matrix added concentration of 50 µg/kg, malachite green and leucomalachite green degradation rate constants were K (50 µg/kg) = d 1, K (50 µg/kg)= d 1 in natural thawing.and K (50 µg/kg) = d 1, K (50 µg/kg) = d 1 in microwave thawing, respectively. The leucomalachite green degradation rate constants in practical samples were K (111.2 µg/kg carp ) = d 1, and K (50 µg/kg carp ) = d 1. Conclusion Degradation rates of malachite green was faster than that of leucomalachite green. Compared with higher * 通讯作者 :,, yongping008@yahoo.com.cn *Corresponding author: CHEN Yong-Ping, Engineer, Superision and Test Center for Fisheries Environment and Quality of Fishery Products of Ministry Agriculture, NO. 442, Jiefangnan Road, Hexi District, Tianjin , China. yongping008@yahoo.com.cn

00125 d 1 结论 ; ; 关键词 : ; ; ; Studies on degradation kinetics of malachite green and leucomalachite green in different aquatic products CHEN Yong-Ping *, ZHANG Su-Qing, LI Chun-Qing, LI Lian-Qing,

2 184 4 concentration, the degradation in low concentration was faster. Two kinds of ways of thawing had no effects on the degradation of malachite green and leucomalachite green. The leucomalachite green degradation rate in practical samples was slower than that in the blank matrix added of leucomalachite. KEY WORDS: malachite green; leucomalachite green; aquatic products; degradation kinetics 1 引言,,,,, [1-2],, [3-6],, [7] [8],,,,, 2 材料与方法 2.1 材料与仪器 :,, ; :, ; Sigma : Agilent 1200( ); ( g) (0.001 g) - ; (, r/min); ( Sigma, r/min); ( ); : 1.0 mg( 0.01 mg) ( ), 10.0 ml, 100 µg/ml (1.0 μg/ml): ( ) 1.0 ml, 100 ml, 实验样品的制备及分组 (5±0.02)g 50 ml ( 150, 90 ), μg/kg, ( d), 50 μg/kg 2.3 分析方法 5.00 g, 1,, 2,, 6000 r/min,, 40 ml,, 50 ml,, 3 ml, 1 ml, SPSS 2.4 测定 : Agilent XDB-C 18, 5 µm, 4.6 mm 150 mm; ; : 50 µl; : 1.2 ml/min; VWD : 588 nm : ( ph 4.5): =20 80(v/v) 3 结果与分析 ng/ml 50 µg/kg,,

KEY WORDS: malachite green; leucomalachite green; aquatic products; degradation kinetics 1 引言,,,,, [1-2],, [3-6],, [7] [8],,,,, 2 材料与方法 2.1 材料与仪器 :,, ; :, ; Sigma : Agilent 1200( ); (0.

3 1, : ng/ml (100 ng/ml) Fig. 1 Chromatogram of MG and LMG (100 ng/ml) 2 50 µg/kg Fig. 2 Chromatogram of MG in carp sample (50 µg/kg),, 100 ng/ml µg/kg d,, 10 µg/kg, 50 µg/kg, 100 µg/kg,, SPSS, F, α<0.01 X t/d, Y t Ct, K µg/kg, : K (10 µg/kg, )= d 1, K (10 µg/kg, )= d 1, K (10 µg/kg, )= d 1 ; K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1 ; K (100 µg/kg, )= d 1, K (100 µg/kg, )= d 1, K (100 µg/kg, )= d 1 ; : K (10 µg/kg, )= d 1, K (10 µg/kg, )= d 1,

µg/kg,, SPSS, F, α<0.01 X t/d, Y t Ct, K 10 50 100 µg/kg, : K (10 µg/kg, )=0.005770 d 1, K (10 µg/kg, )=0.00315 d 1, K (10 µg/kg, )=0.

4 186 4 表 1 鲤鱼组织中有 ( 无 ) 色孔雀石绿降解浓度 - 时间表 Table 1 Concentration-time of MG and LMG in carp matrix (µg/kg) /d MG/LMG LMG MG/LMG LMG MG/LMG LMG 表 2 鳗鲡组织中有 ( 无 ) 色孔雀石绿在降解浓度 - 时间表 Table 2 Concentration-time relationship of MG and LMG in eel matrix (µg/kg) /d MG/LMG LMG MG/LMG LMG MG/LMG LMG 表 3 鳜鱼组织中有 ( 无 ) 色孔雀石绿降解浓度 - 时间表 Table 3 Concentration-time of MG and LMG in mandarinfish matrix (µg/kg) /d MG/LMG LMG MG/LMG LMG MG/LMG LMG

6 81.5 84.7 120 4.5 6.5 27.3 31.4 78.1 81.3 150 3.8 5.9 24.5 29.6 76.4 78.5 180 3.3 5.3 22.8 27.6 72.2 76.6 210 3.0 4.8 20.5 24.5 70.7 74.

5 1, : 187 K (10 µg/kg, )= d 1 ; K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1 ; K (100 µg/kg, )= d 1, K (100 µg/kg, )= d 1, K (100 µg/kg, )= d 1,, Table 4 表 4 鲤鱼组织不同解冻方式及阳性样品中有 ( 无 ) 色孔雀石绿降解浓度 - 时间表 Concentration-time of MG(LMG) by different thawing mode and positive samples in carp matrix (50 µg/kg) t/d Sample 1 Sample 2 MG/LMG LMG MG/LMG LMG LMG LMG Table 5 µg/kg 表 5 鲤鱼鳗鲡鳜鱼组织中不同浓度有无色孔雀石绿 210 d 后降解率表 Degradation rate of different MG(LMG) spiked concentration in carp, eel and mandarinfish matrix after 210 d MG/LMG LMG MG/LMG LMG MG/LMG % LMG 1/LMG 2/LMG ,,, y=c t /C 0, µg/kg 57.9% 48.0% 23.0%, 46.6% 43.4% 19.8%, 50 µg/kg K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1, K (50 µg/kg, )= d 1, K (50 µg/kg, )= d -1,,, K (111.2 µg/kg, )= d 1, K (50 µg/kg, )= d 1,,,

Sample 1 Sample 2 MG/LMG LMG MG/LMG LMG LMG LMG 1 42.4 45.1 41.0 44.4 111.2 54.6 2 41.6 44.5 40.2 43.5 110.5 54.3 4 39.8 43.3 39.3 42.7 109.6 53.9 10 38.5 41.2 38.5 41.8 108.8 53.2 20 36.2 39.1 37.

6 结论,, : µg/kg, 50 µg/kg,, spss,,,,,, 参考文献 [1],,. [J]., 2007, 22(4): Gui YA, Wang HJ, Liu CL. Potential hazards of malachite green residues and itsmetabolites and detection in aquatic animals [J]. J Dalian Fish Univ, 2007, 22(4): [2],,. [J]., 2008, 24 (6): Qu ZN, Li CJ, Zhao SJ. Study on Eliminate of Malachite Green and its Metabolite in Turbot Muscle [J]. Chin Agr Sci Bull, 2008, 24 (6): [3],,. [J]., 2008, 27(8): Li HX, Na GS, He W. Advances in Studies on Malachite Green and Leucomalachite Green [J]. Fish Sci, 2008, 27(8): [4],,. [J]., 2006, 36(5): Qiu XJ, Lin H, Wang LZ. Study on Metabolism of Malachite Green in Crucian (Carassius auratus ) Muscle [J]. Period Ocean Univ China, 2006, 36(5): [5] Liewes E, Haenen O. The veterinary approach to eels [M]. BROWN L. Aquaculture for Veterinarians: Fish Husbandry andmedicine. Pergamon, New York, 1993: [6] Scott P. Therapy in aquaculture [M]. ROWN L. Aquaculture for Veterinarians: Fish Husbandry and Medicine. Pergamon, NewYork, 1993: [7]. [J]., 2009, 2(1): Yu PJ. Degradation of Malachite Green in the Fishery Water by Medicine [J]. J Hydroecol, 2009, 2(1): [8],,. [J]., 2007, 31 : Gao LJ, Cai YQ, Jiang CJ. Accumulation and elimination of malachite green and its primarymetabolite in juvenile European eels (Anguilla anguilla) muscle [J]. J Fish of China, 2007, 31(suppl): ( 责任编辑 : 张宏梁 ) 作者简介陈永平,, yongping008@yahoo.com.cn

Qu ZN, Li CJ, Zhao SJ. Study on Eliminate of Malachite Green and its Metabolite in Turbot Muscle [J]. Chin Agr Sci Bull, 2008, 24 (6): 491 496. [3],,. [J]., 2008, 27(8): 430 432. Li HX, Na GS, He W.

32期

32期中国农学通报 2014,30(32):38-42 Chinese Agricultural Science Bulletin 中草药饲料添加剂对黄鳝性激素含量的影响 1,2 1,2 1,2 2 赵庆凯, 丁炜东, 邴旭文, 王建新 ( 1 南京农业大学无锡渔业学院, 江苏无锡 214081; 2 中国水产