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1 2011 9, 18(5): Journal of Fishery Sciences of China 研究论文 DOI: /SP.J 王静香 1,2, 李加儿 1, 区又君 1, 王刚 1., ; 2., ,2 摘要 : PAS Mallory 3,, (Cromileptes altivelis),,, 2 A 1 A 2 B 2, Ⅰ Ⅱ 3 : (RPD) (PPD) (PI) 6, RPD 2 : (ACTH) (PRL) ; PPD 3 : (GH) (GTH) (TSH) ; PI 1, (MSH), 1 (SC) 6 : 2, PRL ACTH ; GH GTH TSH 3 ; MSH 1 GTH,, : ; ; ; 中图分类号 : Q959; S917 文献标志码 : A 文章编号 : (2011) (Cromileptes altivelis),, (Serranidae) (Epinephelinae) [1],,,,,,, 2004 [2] [3 5] [6] [7] [8] [9] [10 11] [12] [13],, [3] [4] [5] [14], 7 :,,,, 收稿日期 : ; 修订日期 : 基金项目 : 863 (2006AA10A414); (C20817); ( ) (A200501A04). 作者简介 : (1984 ),,.. wangjingxiang501@163.com 通信作者 : (1954 ),. lje001@126.com

2 , 15,,, ( ) cm, ( ) g Ⅲ IV ,,,,,,, %,,, ( 5 6 μm),, PAS Mallory, Olympus, % 12 h, 0.1 mol/l (ph7.4), 1%, 0.1 mol/l (ph7.4),, Epon812,,, Philips CM , ( 1),,,,, (Rostral Pars Distails, RPD), (Proximal Pars Distalis, PPD) (Pars Intermedia, PI)3 ( I 1) PAS 2,, 2 : (Ⅰ ); (Ⅱ )( I 2) Ⅱ Ⅰ,,, ( I 1) 2.2.2, 2 : 1 (prolactin cell, PRL),, MA ; 1 (adrenocorticotrophic cell, ACTH), 1 1. ; 2. ; 3. ; 4. ; 5. ; 6. ; 7. ; 8. ; 9. ; 10. ; 11. ; 12.. Fig.1 Back and ventral aspect of anatomical structure of Cromileptes altivelis brain and pattern view 1. olfactory nerve; 2. olfactory lobe; 3. cerebrum; 4. mesocephalon; 5. cerebellum; 6. spinal cord; 7. inferior lobe; 8. optic nerve; 9. pituitary; 10. infundibulum; 11. saccus vasculosus; 12. medulla oblongata.

3 5 : 1063, PAS, ( I 3), 3 : 1,,, MA, (growth hormone, GH) ; 2, MA,, 2 (gonadotrophic hormone, GTH), (thyrotropic hormono, TSH), ( I 1), GH, TSH, GTH ( I 4),,,, PAS,, MA, (melanophore-stimulating hormone, MSH) ( I 5) (blood antrum),, ( I 6) ,, A 1 A 2 B 3 ( II 1) A 1,,,, A 2,, A 1, A 2 A 1 B,, ( II 2a),,,,, A 1 A 2 B,,,,,,,, ( II 3) ( II 2d),, Ⅰ, ; Ⅱ,,, 1/2,, ( II 4 6) (ACTH), 6 10 µm,,,,,,,, ( II 7) (PRL),, 7 11 µm,,,,,,,, ( II 2b, c), ACTH ( II 8) (GH),,, 6 10 µm,,,,,,,,, ( II 9) (GTH) 8 12 µm,,,, GTH,,

4 ,,,,, 3 :,, GTH,,, ( II 10 12) (TSH), 6 8 µm,,,,,,,,, ( II 13) (MSH),,, 5 8 µm,,,,,, ( II 14) (SC),,,,,,, ( II 2b), ( II 2c), 3 3.1,, 2, [15] [15] VanOodert [16],,,,, 3, ;, 1/2, 3,,,,, 3,, (Pelteobagrus vachelli) [17] (Carassius auratus) [16] (Ctenopharyngdoni- dellus) [16] 3.2 [18] 6 : ACTH PRL GH GTH TSH MSH, ACTH PRL TSH, GH GTH, MSH [19] 6, TSH 6, PAS- -, [20-22], [19] 6 [19], [23],, [24] (Silurus meridionalis) 8 ACTH PRL GTH TSH MSH, 2 GH, GH, GH [24] 6, ACTH PRL GH GTH TSH MSH 2 PRL ACTH ; GH GTH TSH 3 ; MSH 3.3 GTH GTH, GTH 1 GTH [18] 20 70, 2 GTH:

5 5 : 1065 (follicle stimulating hormone, FSH),, ; (luteinizing hormone, LH),, (Cyprinus carpio) [25], (Megalobrama terminalis) [26], (Silurus asotus) [20] Nagahama (Anguilla japonica) 鳉 (Oryzias latipes), 1 GTH,, GTH [18],, 2 GTH [27] 1 GTH,, GTH,, ; GTH,,, GTH, [28 30], GTH GTH,, GTH, 参考文献 : [1],,. [J]., 1999, 6(1): [2],. [M]. :, 2004: 61. [3],. [J]., 2008, 15(5): [4],,,. [J]., 2009, 30(2): [5],,,. [J]., 2010, 32(4): [6],,,. [J]., 2007, 31(5): [7],,. [J]., 1999, 19(3): [8] Rossita S, Wing-Keong N, Saleem M. Replacement of fish meal with poultry by-product meal in diets formulated for the humpback grouper, Cromileptes altivelis[j]. Aquaculture, 2007, 273 (1): [9],,. [J]., 1995, 17 (3 4): 63. [10],. [J]., 2007, 3(5): [11],,,. 5 RAPD [J]., 2008, 4(2): [12] Reynolds W W, Casterlin M E. Thermoregulatory behavior juvenile Cromileptes altivelis (Serranidae) a tropical marine fish [J]. Hydrobiologia, 1980, 75(3): [13]. [J]., 2006, 30(8): [14],,,. [J]., 2011, 35(4): 77 82, 88. [15]. [M]. :, 2000: [16],,,. [J]. :, 2008, 32(3): [17],,,. [J]. :, 2004, 29(1): [18],,,. [M]. :, 1990: [19]. [M]. :. 2000: , [20],. [J]., 2007, 26(3): [21],. PAS [J]. :, 1991( ): [22],,,. [J]., 2003, 10(1): 1 5. [23],,. 鳠 [J]., 2010, 25(2): [24],,,. [J]. :, 2010, 32(10): [25] Polenov A L, Belenky M A, Kornienko G G, et al. The hypo-

6 thalamo-hypophysial system of the wild carp, Cyprinus carpio L I: structure and ultrastructure of the posterior neurohypophysis[j]. Cell Tissue Res, 1984, 237(1): [26],,. [J]. :, 2003, (4): [27],,,. : IV. GTH GTH [J]., 1987, 11(4): [28] Weltzien F A, Norberga B, Helvikb J V, et al. Identification and localization of eight distinct hormone-producing cell types in the pituitary of male Atlantic halibut (Hippoglossus hippoglossus L.) [J]. Comp Biochem Physiol, 2003, 134(2)A: [29]. [J]., 1990, 9(1): [30],. [J]. :, 2008 (4): Microstructural and ultrastructural study of pituitary in highfin grouper Cromileptes altivelis WANG Jingxiang 1,2, LI Jiaer 1, OU Youjun 1, WANG Gang 1,2 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou , China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai , China Abstract: Pituitary was one of most complicated endocrine gland, the function of it was diversification. Pituitary could modulate the activities of the body organs and tissues. It was necessary to investigate structure of fish pituitary in order to provide the basis for histological morphology, reproductive biology and pathology. Using improved staining, Mallory and PAS staining method, and transmission electron microscopy technique, the microstructure and the ultrastructure of the pituitary of Cromileptes altivelis was examined. Results showed that: the pituitary of the C. altivelis was a solid spherical glands, located in the ventral diencephalon, and was composed of neurohypophysis and adenohypophysis. According to the shape and size of excretive granule contained in nerve fibers, the neurosecretory fibers could be differentiated into A 1, A 2 and B-type. Two types of pituitary cells could be observed in the neurohypophysis, I-type pituitary cells and II-type pituitary cells. Adenohypophysis consistsed of three parts: rostral pars distails(rpd), proximal pars distalis(ppd) and pars intermedia(pi). It could be identified six endocrine cells in adenohypophysis. Adrenocorticotrophic (ATCH) cells and prolactin (PRL) cells existed in the RPD, growth hormone (GH) cells, thyrotrophs (TSH) cells and gonadotrophic hormone (GTH) cells could be found in the PPD, only one endocrine cell were observed, it was melanophore-stimulating hormone (MSH) cells. And one non-secretory cell type-stellate cells (SC) could also be seen in adenohypophysis. The sort of gonadotrophic hormone cells and the composition and distribution of endocrine cell of adenohypophysis were discussed. There was only one kind of GTH cells, but they had many forms. The endoplasmic reticulum of some GTH cells significantly expanded, foam based, and it relatively had a few secretory granules in the cytoplasm. Since the release of secretion of some GTH cells, nuclear was smaller, degenerate, or even disassemble, and secretory granules was preternaturally rich in the cytoplasm. There also had many GTH cells that were not found vacuole, and the edge of cell showed lace-shaped or notch, mitochondria well-developed. Key words: Cromileptes altivelis; pituitary; microstructure; ultrastructure Corresponding author: LI Jiaer. lje001@126.com

7 5 : 1067 图版 Ⅰ 驼背鲈脑垂体的显微结构 1 Mallory, : (RPD), (PPD), (PI), (NH).2. PAS, Ⅰ (Ⅰ), Ⅱ (Ⅱ).3. PAS, (ACTH), (PRL). 4., (GH) ; (GTH) ; (TSH). 5. PAS, (MSH). 6. PAS, (En). Plate Microstructure of pituitary of Cromileptes altivelis 1. Mallory stained method, showing morphology and subarea of pituitary: rostral pars distails(rpd), proximal pars distails (PPD), pars intermedia (PI), neurohypophysis(nh); 2. PAS stained method, showing typeⅠpituicyte(Ⅰ), typeⅡpituicyte (Ⅱ); 3. PAS stained method, showing adrenocorticotrophin (ACTH) cell, prolactin (PRL) cell; 4. improved staining, showing growth hormone (GH) cell, gonadotrophin (GTH) cell, thyrotropin (TSH) cell, 5. PAS stained method, melanophore-stimulating hormone (MSH) cell. 6. PAS stained method, showing endothelium (En). 图版 Ⅱ 注释 Explanation of plate II 1. A 1 (A 1 ), A 2 (A 2 ), B, (Sg); 2. (SC), (DSC), (PC), (HB), (nt); 3., (En), (BC), (Sg), (cf); 4. Ⅰ (Ⅰ), (M), (CM); 5. Ⅱ (Ⅱ), (rer); 6. Ⅰ, Ⅱ (Ⅱ); 7. (ACTH) ; 8. (PRL) ; 9. (GH) ; 10. (GTH) ; 11. (GTH) ; 12. (GTH) ; 13. (TSH) ; 14. (MSH). 1. showing type A 1 neurosecretory fibre(a 1 ), type A 2 neurosecretory fibre(a 2 ), type B neurosecretory fibre(b), elementary ganules(eg); 2. stellate cells (SC), dividing stellate cells (DSC), podgy chromosome(pc), herring body (HB), neurotubule(nt); 3. blood antrum; showing endothelium (En), blood cell (BC), secretory granules(sg), microfilament(cf); 4.typeⅠpituicyte (Ⅰ), mitochondrium of cytoplasm (M), cell membrane(cm); 5. typeⅡpituicyte (Ⅱ), the rough endoplasmic reticulum (rer) around nucleolus; 6. typeⅠpituicyte (Ⅰ), typeⅡpituicyte (Ⅱ); 7.adrenocorticotrophin (ACTH) cell; 8. prolactin (PRL) cell; 9. growth hormone (GH) cell; 10. gonadotrophin (GTH) cell; 11. gonadotrophin (GTH) cell; 12. gonadotrophin (GTH) cell; 13. thyrotropin (TSH) cell; 14. melanophore-stimulating hormone (MSH) cell.

8 图版 Ⅱ 驼背鲈脑垂体的超微结构 Plate Ultrastructue of pituitary of Cromileptes altivelis Explanation of plate II is shown in page 1067.

Microsoft Word 曹伏君_new_.doc

Microsoft Word 曹伏君_new_.doc 45 2 Vol.45, No.2 2014 3 OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2014 (Lethrinus erythropterus) * ( 524025) Wright (Lethrinus erythropterus),,,, ; ; ; Q954.56 doi: 10.11693/hyhz20140100011 (Lethrinus erythropterus)