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1 驼背鲈外周血细胞的形态学研究 1,2, 1, 1 (1., ; 2., ) 摘要 : 利用 Wright s 和 Giemsa 双重染色法和改良的血涂片染色法两种方式, 通过光镜对驼背鲈 (Cromileptes altivelis) 的血涂片进行了观察结果表明 : 驼背鲈的血涂片中可区分出 6 种细胞红细胞嗜中性粒细胞嗜酸性粒细胞单核细胞淋巴细胞血栓细胞, 偶尔可见到分裂的红细胞分裂的血栓细胞核影红细胞和聚集的白细胞此外, 统计了红细胞数个 / mm 3, 白细胞数个 / mm 3, 并对白细胞进行了分类计数 : 淋巴细胞 37.26% 血栓细胞 30.82% 嗜中性粒细胞 23.57% 单核细胞 8.35% 嗜酸性粒细胞极少见描述了以上各细胞的显微结构单核细胞的体积最大, 小淋巴细胞的体积最小 ; 淋巴细胞的数量最多, 嗜酸性粒细胞数量最少关键词 : 驼背鲈 (Cromileptes altivelis); 血细胞 ; 形态学中图分类号 : Q 文献标识码 : A 文章编号 : (2011) , 2% [1],,, Valenzuela [2] Ballarin [3] Gao [4] (Oncorhynchus mykiss)(umbrina cirrosa) (Misgurnus anguillicaudatus) ; [5] [6] [7] (Boleophthalmus pectinirosris) (Rachycentron canadum) ; (Lutjanus erythopterus) [8] (Chaenocephalus aceratus) [9] (Huso dauricus) [10] (Anguilla anguilla) [11] (Oplegnanathus fasciatus) [12] (Carassius auratus) [13] 鲹 (Trachinotus ovatus) [14] [15] [16] [17] b [18], (Cromileptes altivelis),, (Serranidae)(Epinephelinae) (Cromileptes) [15],, 1,,,,,,, 1 材料与方法 1.1 材料 ,, (26.48±1.08) cm, (465.75±97.96) g/ 1.2 方法 %,,, 1.2.2, 0.65 % NaCl 100, ; 2 ml, 98 ml, 10 g/l : ; : : (C20817); ( ) (A200501A04) : (1984-),,,,, : , wangjingxiang501@163.com;,,, : , lje001@126.com Marine Sciences / Vol. 35, No. 4 /

3 10 1.2.3, 10, 5 Wright s Giemsa, 5 95 % 10 min, 5 min, 5 min, 2 min,,, 5 ( Wright s Giemsa ), 100, [6] 2 916 Olympus,, ( ) 1.2.4 ±, Excel SPSS 2 结果 2.1 血细胞的测定及分类计数, 1 1, : 37.26%, 30.82%, 23.57%, 8.

2 , 10, 5 Wright s Giemsa, 5 95 % 10 min, 5 min, 5 min, 2 min,,, 5 ( Wright s Giemsa ), 100, [6] Olympus,, ( ) ±, Excel SPSS 2 结果 2.1 血细胞的测定及分类计数, 1 1, : 37.26%, 30.82%, 23.57%, 8.35%,, /mm 3, /mm 3 表 1 驼背鲈血细胞的大小和白细胞的分类统计值 Tab. 1 Sizes of the blood cells and classified statistics of white blood cells of Cromileptes altivelis ( ) ( ) (%) (µm) (µm) (9.82±0.88) (7.26±0.77) (4.59±0.73) (3.19±0.64) (8.78±0.99) (7.21±0.87) (8.19±0.71) (7.26±0.95) (7.07±1.32) (5.58±0.93) (5.72±0.73) (4.74±0.90) (4.70±1.08) (3.85±0.74) (3.89±0.40) (3.34±0.63) 8.35 (10.99±2.7) (9.61±1.99) (7.95±2.02) (5.85±1.83) (5.50±2.72) (3.33±0.64) (4.14±1.61) (2.84±0.52) (9.17±2.67) (7.21±2.16) (6.74±2.11) (4.76±1.26) 2.2 红细胞 ( 1-1),,, ( )( 1-2), 1,,, ( 1-3),,, Wright s Giemsa,,, ( 1-4) ( 1-5, 1-6) ( 1-7),,,,,,,, 1 Fig. 1 Erythrocyte of Cromileptes altivelis 1-1 (ER); 1-2. (SNE); 1-3. (IER); 1-4. (DE); 1-5. (TE); 1-6. (TE); 1-7. (NSE); 1-8. (DIE); 1-3,1-7. Wright s Giemsa, 1-1. erythrocyte (ER); 1-2. sub-leaf nuclear erythrocyte(sne); 1-3. immature erythrocyte (IER); 1-4. dissolving erythrocyte (DE); 1-5. transitional erythrocyte (TE); 1-6. transitional erythrocyte (TE); 1-7. nuclear shadow of erythrocyte (NSE); 1-8. dividing erythrocyte (DIE);1-3, 1-7. were dyed according to the Wright-Giemsa double staining method, and others were used by improved blood smears staining method 78 / 2011 / 35 / 4

6 血栓细胞,,,,,, ( 4-5)( 4-6) ( 4-7)( 4-8),, ( 4-9) 3 讨论 3.1 几种鱼类血细胞数量的比较 2 Fig. 2 Granulocyte of Cromileptes altivelis 2-1.(EG); 2-2.(NE); 2-3. (NE); 2-4.(NE); 2-5.(LA); 2-6.

3 ,,, ( 1-8) 2.3 粒细胞, ( 2-1) ( 2-2,2-3,2-4),,,,,,,, ( 2-5, 2-6) 2.4 单核细胞, ( 3-1),, ( 3-2),, 1/3 1/2 3 Fig. 3 Monocyte of Cromileptes altivelis 3-1.(MO); 3-2.(MO); 3-3.(MO); Wright s Giemsa 3-1. monocyte (MO); 3-2. monocyte (MO); 3-3. monocyte (MO); all were dyed according to the Wright-Giemsa double staining method.,,, ( 3-3) 2.5 淋巴细胞 3 ( 4-1, 4-2, 4-3):,,,,,,, ( 4-4) 2.6 血栓细胞,,,,,, ( 4-5)( 4-6) ( 4-7)( 4-8),, ( 4-9) 3 讨论 3.1 几种鱼类血细胞数量的比较 2 Fig. 2 Granulocyte of Cromileptes altivelis 2-1.(EG); 2-2.(NE); 2-3. (NE); 2-4.(NE); 2-5.(LA); 2-6. (GA); 2-5, Wright s Giemsa 2-1.eosinophilic granulocyte (EG); 2-2. neutrophilic granulocyte (NE); 2-3. neutrophilic granulocyte (NE); 2-4. neutrophilic granulocyte(ne); 2-5. leukocyte aggregation (LA); 2-6. granulocyte aggregation (GA); 2-5. was used by the improved blood smears staining method, and others were dyed by the Wright-Giemsa double staining. 5,,, [19], ( ), [11],,, Marine Sciences / Vol. 35, No. 4 /

4 4 Fig. 4 Lymphocyte and thrombocyte of Cromileptes altivelis 4-1. (LL); 4-2. (ML); 4-3. (SL); 4-4. (SSL); 4-5. (TST); 4-6. (SST); 4-7. (TT); 4-8. (ST); 4-9. (DIT); 4-9., Wright s Giemsa 4-1. large lymphocyte (LL); 4-2. middle lymphocyte (ML); 4-3. small lymphocyte (SL); 4-4. sickle-shaped lymphocyte (SSL); 4-5. tadpole shape thrombocyte (TST); 4-6. spindle shape thrombocyte (SST); 4-7. teardrop thrombocyte (TT); 4-8. short stick shape thrombocyte (ST); 4-9. dividing thrombocyte (DIT); 4-9. were dyed by improved blood smears staining method and others were used by the Wright-Giemsa double staining method, ;, [6] [10] 鲹 [14] (Ictalurus punctatus) [20] (Hypophthalmichys molitrix) [21] 2 鲹, ; 鲹, :, 鲹 (P 0.05);, 鲹鲹 (P 0.05), (P 0.05), [22] ( ) 3.2 驼背鲈红细胞的形态特点及其功能 [11] [12] 鲹 [14] (Plectorhinchus cinctus) [23] (Acipenser schrencki) [24] (Muraenesox cinereiu) [19],,, Ellis [25], 表 2 不同鱼类血细胞数量的比较 Tab. 2 Comparison of the blood cell counts among different fish species (g) (cm) (10 4 /mm 3 ) (10 4 /mm 3 ) 465.7± ± a 0.48 a, b 0.69 ab, [20] 鲹 537.5± ± c 1.20±0.25 bc [14] (2.97±0.82) 10 5d (1.39±0.94) 10 2c [6] ±12.37 e 2.73±0.82 d, [10] 340.3± ±5.3 (2.02±29.1) 10 2f 2.49±0.7 d, [21] :, ; 80 / 2011 / 35 / 4

5 ,,,,,, [26],,,,,,, ;,, [8] [19] 3.3 驼背鲈白细胞的形态特点及其功能 ,, [27],,,,, Watson [28], [29] 100 (Claris lareza) (Carassius auratus var.) [28], (Hemibarbus maculatus) [30], 3.3.2, Ellis [25], ; Barber [9],, [31],,,,, [32],, : [13],,,, [8] [11] [12] 3.3.3,,,, 4 : [6], [11] 5, [33] (Carassius auratus cuvieri) [34] 4, [35] Barber [9],,,,, [12] 鲹 [14] ;, (Aritichthys nobilis) [36] : [1]. [M]. :, 2000: [2] Valenzuela A E, Silva V M, Klempau A E. Some changes in the haematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to three artificial photoperiod regimes [J]. Fish Physio Biochem, 2007, 33(1): [3] Ballarin L, Dall Oro M, Bertotto D, et al. Haematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): a comparison between diploid and triploid specimens[j]. Comp Biochem Physiol, 2004, 138A: [4] Gao Z, Wang W, Abbas K, et al. Haematological characteri-zation of loach Misgurnus anguillicaudatus: comparison among diploid, triploid and tetraploid specimens [J]. Comp Biochem Physiol, 2007, 147(4): [5],,,. Marine Sciences / Vol. 35, No. 4 /

6 [J]., 2002, 8(1): [6],,,. [J]., 2005, 29(5): [7],,,. [J]., 2007, 3(1): [8],. [J]., 2006, 30(5): [9] Barber D L, Mills Westermann J E, White M G. The blood cells of the Antarctic icefish Chaenocephalus aceratus Lönnberg: light and electron microscopic observations [J]. J Fish Biol, 1981, 19(1): [10],,,. [J]., 2006, 13(3): [11],,,. [J]., 2002, 48(3): [12],,,. [J]. ( ), 2009, 38(4): [13],,. [J]., 1985, 6(2): [14],,,. 鲹 [J]., 2008, 43(6): [15],,. [J]., 1999, 6(1): [16]. [J]., 2006, 30(8): [17] Rossita S, Wing Keong N, Saleem M. Replacement of fish meal with poultry by-product meal in diets formulated for the humpback grouper, Cromileptes altivelis[j]. Aquaculture, 2007, 273(1): [18],,, b [J]., 2008, 4(5): [19],. [J]., 2003, 38(6): [20],,. [J]. ( ), 2004, 30(4): [21]. [J]., 1982, 9(4): [22],,,. [M]. :, 1990: [23] Fujun C, Chuwu L, Changling L. Microstructure of peripheral blood in Plectorhinchus cinctus [J]. Acta Laser Biology Sinica, 2001, 10(3): [24] Hongbai L, Yuping H, Qiuzhi Q, et al. Microstructuer and ultrastructure of peripheral blood cells of amur sturgeon Acipenser schrencki brandt[j]. Acta Hydrobiologica Sinica, 2006, 30(2): [25] Ellis A E. Bizarre forms of erythrocytes in a specimen of plaice, Pleuronectes platessa L.[J]. J Fish Dis, 1984, 7(5): [26],. [J]., 1993, 17(1): [27]. [M]. :, 1999: [28] Watson L J, Shechmeister I L, Jackson L L. The haematology of goldfish, Carassius auratus[j]. Cytologia, 1962, 28: [29],,. [J]., 1991, 37(3): [30],,,. [J]., 2009, 37(22): , [31]. [J]., 1996, 42(2): [32] Morrow W J W, Pulsford A. Indentification of peripheral blood leucocytes of the dogfish (Scyliorhinus canicula L.) by electron microscopy[j]. J Fish Biol, 1980, 17(4): [33] E1lis A E. The leucocytes of fish: a review [J]. J Fish Biol, 1977, 11(5): [34],. [J]., 1996, 31(11): [35] Burrows A S, Fletcher T C, Manning M J. Haematology of the turbot, Psetta maxima(l.): ultrastructural, cytochemical and morphological properties of peripheral blood leucocytes[j]. J Appl Ichthyol, 2001, 17(2): [36],,,. [J]., 1988, 22(1): ( 下转第 88 页 ) 82 / 2011 / 35 / 4

7 ( 上接第 82 页 ) Morphological study of the peripheral blood cells of highfin grouper Cromileptes altivelis WANG Jing-xiang 1,2, LI Jia-er 1, OU You-jun 1 (1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou , China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai , China) Received: Apr., 7, 2010 Key words: Cromileptes altivelis; blood cells; morphology Abstract: Using the Wright-Giemsa double staining method and improved blood smears of two staining methods, the peripheral blood cells of the Cromileptes altivelis were studied by light microscopy. Six major types of cells were recognized: erythrocyte, neutrophil, eosinophilic granuloeyte, monocyte, lymphocyte, and thrombocyte. In blood smears, erythrocyte division, divided thrombosis cells, the shadow of erythrocyte, and leukocyte aggregation could be observed. The quantities of erythrocytes and leukocyte were / mm 3 and /mm 3, respectively. The percentages of lymphocytes, thrombocyte, neutrophil and monocytes were 37.26%, 30.82%, 23.57% and 8.35% respectively, while eosinophilic granuloeyte was few. The microstructure of the above cells was described. The size of small lymphocyte was the smallest while that of the monocyte was the largest; the number of neutrophil was the least while that of lymphocytes was the most in the blood cells. ( 本文编辑 : 谭雪静 ) 88 / 2011 / 35 / 4

Microsoft Word 曹伏君_new_.doc

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