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1 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 2011 年第 30 卷第 1 期 : 海洋生物学鱾肖云朴 1, 徐善良 2, 孙敏 3, 吕慧明 3 (1., ; 2., ; 3., ) : Girella leonina : 1) 6 2), 10.00μm 6.40μm, 8.80μm 5.80μm,, 3.20μm 2.60μm, 2.80μm 2.50μm;,,,, 3),,,, 4,, 4),,,, 9+2 : Girella leonina; ; ; 中图分类号 : Q 文献标识码 : A 文章编号 : (2011) Ultrastructural changes in Girella leonina during spermatogenesis XIAO Yun-pu 1, XU Shan-liang 2, SUN Min 3, LÜ Hui-ming 3 (1. Ocean and Fisheries Bureau of Pingyang County, Pingyang , China; 2. Key Laboratory of Marine Biotechnology of Zhejiang, Ningbo , China; 3.College of Life Sciences, Ningbo University, Ministry of Education, Ningbo , China) Abstract: The ultrastructural changes of the germ cells and sperm during spermatogenesis of Girella leonina were observed by transmission electronic microscope. The results are as follows. (1) The spermatogenesis of Girella leonina included six stages: primary spermatogonium, secondary spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and sperm. These stages can be divided into three periods (before, during, and after) when the spermatid differentiated into sperm. (2) A primary spermatogonium was the largest of various stages of germ cells, and its cell was about 10.00μm 6.40μm and the nuclear counted 8.80μm 5.80μm. The spermatid was the smallest, and its cell was about 3.20μm 2.60μm and the nuclear counted 2.80μm 2.50μm. With spermatogenic cells continued to divide and mature, their size gradually reduced, the nuclear chromatin tended to concentrate, electron density increased, extent of staining deepened, and nuclear position, nucleolus, mitochondria also correspondingly changed. (3) Mitochondria were the main organelles in the germ cell, which gradually reduced the number in the process of spermatogenesis. At last, there were only four mitochondria in the mature sperm. On the contrary, the volume of mitochondria had kept increasing, and its cristae became clearer and longer. (4) As most kinds of teleostean, each sperm of Girella leonine consisted of a head without an acrosome, a midpiece, and a long tail that contains the lateral fins and a conventional axoneme pattern. Key words: Girella leonina; spermatogenesis; ultrastructural; mitochondria 收稿日期 ; 修订日期基金项目 (2007C22044) 作者简介 (1975 ),,,, xiaoyunpu@21cn.com 通信作者 : Tel: , xushanliang@nbu.edu.cn

2 108 Vol. 30, No. 1 / Jan., 2011 Girella leonina,, 鱾,, [1],,,, [2] 鱾 [3] [4] [5], [6 7],,, , cm, g 1.2,, 1mm 3, 0.1mol L -1 ph % (4 ), 1 1 2h,, Epon 812, POWERTOME XL,, H =( )/( ) [8] ,,, ;,, 初级精原细胞, 10.00μm 6.40μm,, 8.80μm 5.80μm( 1a), 0.798,,,,, ( 1a), ( 1b) ( 1c) 次级精原细胞, 9.00μm 6.20μm, 6.40μm 5.60μm, 0.642,, ( 1d 1e),,,,,,,, 初级精母细胞 ( 1f),, 7.50μm 3.20μm,, 3.80μm 2.60μm, 0.412,,, ( 1g),,, ( 1h),, ( 1h 1i),,,,,,,,

3 : a., ; b., ; c. ; d., ; e. ; f. ; g., ; h., ; i. ; j.,, ; k. ; l., ; m., ; n. ; o., G1: ; G2: ; ER: ; M: ; N: ; NU: ; SC: ; SE: ; SG1: ; SG2: Fig. 1 Ultrastructural observation in Girella leonina during early spermatogenic cell

4 110 Vol. 30, No. 1 / Jan., 2011, 3 4 ( 1i),,,,, ( 1j),,,, ( 1k),,,,,, ( 1l) 次级精母细胞, ( 1n), 4.50μm 3.50μm, 3.30μm 2.70μm,,, ( 1m),,,, ( 1o) 精子细胞 ( 2a),, 3.2μm 2.6μm, 2.80μm 2.5μm( 2b),, 0.841,, ( 2c),,, ( 2d) 精子形成早期,, ( 2c),, ( 2e),, ( 2f 2g) ( 2d),,,, ( 2h, 2i) 精子形成中期,, ( 2j 2k), ( 2j),, ( 2k), ( 2j) 精子形成晚期,, ( 2m),,,, ( 2o) 成熟精子结构 3, μm,,,,, 1/2 ( 2m 2n), ( 2m), 9+2 ( 2l),, ( 2o) 3 3.1,,,, Sparus macrocephalus Rhabolosargus sarba [9],,, [9 11],,,, 魢,

5 : a. ; b., ; c., ; d., ; ; e., ; f., ; g. 2e, ; h.,, ; i., ; j., ; ; k., ; ; l., ; m., 4 ; n., ; o., ; Ax: ; DC: ; ER: ; F: ; G: ; G1: ; G2: ; H: ; IF: ; LF: ; M: ; MP: ; MT: ; N: ; NU: ; NV: ; PC: ; PD: ; PS: ; PP: ; S: ; SC: ; SD: ; SE: ; SG1: ; SG2: ; SP: ; V: Fig. 2 Ultrastructural changes in Girella leonina during spermatogenesis

6 112 Vol. 30, No. 1 / Jan., 2011 鱾,, 鮠 Leiocassis crassilabrus Günther [12] Pseudobagrus fulvidrico [13] Poecilia reticulata [14] 3.2,,, 4,,,, [15],,,,,,, 鱾, [16] Pseudosciaena crocea [9] [14] 鲉 Sebastiscus marmoratus [11] 3.3 3, [16] [14] Cynoglossus semilaevis Günther [17],, ;,, 魢,,,, 3, [9 15], 4,, 1/2,, [15],,,, [1]. [M]. :, 1993, 12: [2],. Girella punctata [J]., 1998, 7 (2): [3],. [J]., 2008, 35(1): 1 8. [4],,,. 鱾 (Girella leonina) [J]. :, 2009(3): [5],,. [J]., 2009, 28(4): [6] NAOKI Y, TETSUJI N. Revision of the genus Girella (Girellidae) from East Asia [J]. Ichthyol Res, 1999, 47(2): [7] NAOKI Y, TETSUJI N. Evolutionary trend in feeding habits of Girella[J]. Ichthyol Res, 2003, 50: [8],,. [J]., 2006, 13(2): [9],,. [J]., 2001, 22(6): [10],,,. 3 [J]., 2007, 38(2): [11],. 鲉 [J]., 1998, 19(5): [12],,,. 鮠 [J]., 2008, 38(1): [13],. [J]. :, 2004, 27(1): [14],,,. [J]. :, 2006, 26(6): [15] MEDINA A, MEGINA C, ABASCAL F, et al. The sperm ultrastructure of Merlucciusmerluccius (Teleostei Gadiformes): phylogenetic considerations[j]. Acta Zoologica, 2003, 84: [16],,. [J]., 2005, 29(10): [17],,,. [J]., 2008, 54(2):

1190 18 :, ;, 2.5% 1%; Epon-812 ; LKB ; ; H-7000 2 2.1,, ( 1 2), ( 3),, ( 4),,, ( 1 2) 2.2, 螠 5, 2.2.1 6 7 11 1, 200~600 μm,, ( 3),,, 9 15, 螠,, 2.2.2

1190 18 :, ;, 2.5% 1%; Epon-812 ; LKB ; ; H-7000 2 2.1,, ( 1 2), ( 3),, ( 4),,, ( 1 2) 2.2, 螠 5, 2.2.1 6 7 11 1, 200~600 μm,, ( 3),,, 9 15, 螠,, 2.2.2 2011 9, 18(5):1189 1195 Journal of Fishery Sciences of China 研究简报 DOI: 10.3724/SP.J.1118.2011.01189 螠王航宁, 邵明瑜, 张志峰,, 266003 摘要 : 螠 (Urechis unicinctus),,,,,,,,, 螠 5, 螠 : 螠 ; ; ; 中图分类号 :