:, ;, 2.5% 1%; Epon-812 ; LKB ; ; H ,, ( 1 2), ( 3),, ( 4),,, ( 1 2) 2.2, 螠 5, , 200~600 μm,, ( 3),,, 9 15, 螠,, 2.2.2

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1 2011 9, 18(5): Journal of Fishery Sciences of China 研究简报 DOI: /SP.J 螠王航宁, 邵明瑜, 张志峰,, 摘要 : 螠 (Urechis unicinctus),,,,,,,,, 螠 5, 螠 : 螠 ; ; ; 中图分类号 : S917 文献标志码 : A 文章编号 : (2011) , 螠 (Urechis unicinctus Von. Drasche) 螠 (Echiurida) 螠 (Urchidae),, [1] [2] [2 3] [4 7], [1],,, 螠, 螠, 螠螠 , 1, 70 10~20 cm 100~300 g 螠,, 1.2 : 螠, Bouin 24 h,,, 5~7 µm, HE, Nikon E80i, 5, 20 收稿日期 : ; 修订日期 : 基金项目 : ( ). 作者简介 : (1983 ),,,. runningbit@yahoo.com.cn 通信作者 :,. zzfp107@ouc.edu.cn

Drasche) 螠 (Echiurida) 螠 (Urchidae),, [1] [2] [2 3] [4 7], [1],,, 螠, 螠, 螠 1 1.1 螠 2009 3 2010 3, 1, 70 10~20 cm 100~300 g 螠,, 1.

2 :, ;, 2.5% 1%; Epon-812 ; LKB ; ; H ,, ( 1 2), ( 3),, ( 4),,, ( 1 2) 2.2, 螠 5, , 200~600 μm,, ( 3),,, 9 15, 螠,, , 800~900 μm, ( 4) ,, ,,,, ;, 1 10,,,, 8 4,,,, 3 ( 5),, (3.5±0.5) µm(n=20),, μm,,,, ( 6),, 2~3 ( 7), 48.8 µm,, 螠 μm,,, ( 8) 螠,, , 100~200 μm,,, 5~10 ( 9), ,, (10.3±0.5) μm(n=20);,

5) µm(n=20),, 2.2.3 8 9 4 5 900 μm,,,, ( 6),, 2~3 ( 7), 48.8 µm,, 螠 2.2.4 9 10 5 6 600 μm,,, ( 8) 螠,, 2.

3 5 : μm,, 0.825, ( 3) 2.3.2,, (7.5±0.5) μm(n=20);, (5.3±0.4) μm(n=20),, 0.7,, (4.8±0.5) μm(n=20), ( 4),,,, ;,,,, ( 1),, ( 2 3) 2.3.3,,, (1.6±0.2) µm,, ( II 4~9),,,, ( 4), ( 5),,,, ( 6 7),, ( 8),,,, 9+2, ( 9) 3 3.1,,,, 螠,,,,,,,,,, 螠, [8],,,,, [9], 螠,,,,,, [9] ; 螠,,,,, 螠螠, 螠,,, [10], 螠

4 , 900 μm,, 螠 [2] 螠,,,,, 螠 5 :,, 螠,,, 螠 3.2,,,,,, :, ;, [11], 螠,,,,,,,,,, [12] 螠,,,,, 4~5 [13 14], [10] 螠 1,,,,,,,,,, 1,,, 参考文献 : [1],,. 螠 ( 螠 ) [J]., 1994, 24 (2) : [2],,,. 螠 [J]., 1997, 43 (4) : [3],,. I [J]., 2002, 32 (2) : [4] Ohkawa Y M, Ishida J. Electron microscopical studies on the egg of the gephyrean Urechis unicinctus [J]. Bull Mar Biol Sta Asamush, 1973, 11 : [5] Sawada N, Noda Y. An electron microscope study on the Urechis egg [J]. Mem Ehim Univ Sect II Ser B, 1969, 4 : [6] Meredith G S. Oocyte differentiation in Urechis caupo (Echiura): A Fine structural study[j]. J Morphol, 1975, 147 : [7],,. [J]., 2005, 12(5): [8],,,.

Electron microscopical studies on the egg of the gephyrean Urechis unicinctus [J]. Bull Mar Biol Sta Asamush, 1973, 11 : 191 192. [5] Sawada N, Noda Y.

5 5 : 1193 [J]., 2006, 30 (1) : [9],,,. [J]., 2007, 53 (4): [10] Luecht J, Pfannenstiel H D. Spermatogenesis in Platynereis massiliensis (Polychaeta: Nereidae) [J]. Helgolander Mar Res, 1989, 43 (1) : [11]. [M]. :, [12]. [M]. :, [13] Rao X, Chen Y, Chen W, et al. Ultrastructural studies on spermatogenesis of Novaculinn chinensis [J]. Chin J Zool, 2000, 35 (5) : 2 5. [14] Zizzari Z V, Lupetti P, Mencarelli C, et al. Sperm ultrastructure and spermiogenesis of Coniopterygidae (Neuroptera, Insecta) [J]. Arthrop Struct Dev, 2008, 37: Annual development of testis and spermatogenesis in Urechis unicinctus WANG Hangning, SHAO Mingyu, ZHANG Zhifeng Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao , China Abstract: We documented the histological structure and annual development of the testis and the process of spermatogenesis in Urechis unicinctus using light microscopy and transmission electron microscopy. The testis was localized at the end of the body and formed a band. Each end of the testis was connected to the body wall and respiratory gut wall by muscle tissue. The testis was composed of interior connective tissue and spermatogenic cells, including spermatogonia clusters at the periphery of the connective tissue and interior spermatocytes surrounded by connective tissue. Spermatocytes that were clustered at different stages were transported into the coelomic fluid and developed into sperm, which left the cell clusters and entered the nephridia. Based on the histological characteristics of the testis, we divided the annual development into 5 stages: proliferative, growing, mature, release, and resting stages. In addition, we observed spermatogenesis in the testis and coelom using both optical and electron microscopy and documented ultrastructural changes in the nucleolus, acrosome, and sperm tail. Key words: Urechis unicinctus; testis; annual development; spermatogenesis Corresponding author: ZHANG Zhifeng. zzfp107@ouc.edu.cn

Chin J Zool, 2000, 35 (5) : 2 5. [14] Zizzari Z V, Lupetti P, Mencarelli C, et al. Sperm ultrastructure and spermiogenesis of Coniopterygidae (Neuroptera, Insecta) [J].

6 图版 Ⅰ 单环刺螠精巢年周期发育的组织学观察 1:, ; 2:, ; 3: ; 4: ; 5:, ; 6: ; 7:, ; 8: ; 9:. BW: ; CF: ; CT: ; F: ; MT: ; RG: ; S: ; SC1: ; SC2: ; SG: ; SP: ; Te:. PlateⅠ Histological observation of Urechis unicinctus testis during annual development 1: testis, connected with body wall; 2: testis, connected with respiratory gut; 3: testis at proliferative stage; 4: testis at growing stage; 5: coelom fluid at growing stage, insert is coelom extract by optical microscope; 6: testis at mature stage; 7: coelom fluid at mature stage, insert is coelom extract by optical microscope; 8: testis at release stage; 9: testis at resting stage. BW: body wall; CF: collagenous fiber; CT: connective tissue; F: flagellum; MT: muscle tissue; RG: respiratory gut; S: somatic cell; SC1: primary spermatocytes; SC2: secondary spermatocytes; SG: spermatogonia; SP: spermatid; Te: testis.

stage; 4: testis at growing stage; 5: coelom fluid at growing stage, insert is coelom extract by optical microscope; 6: testis at mature stage; 7: coelom fluid at mature stage, insert is coelom

7 5 : 1195 图版单环刺螠体腔液中精子发生的超微结构观察 1: ; 2 3: ; 4 8: ; 9:. AC: ; C: ; CB: ; ER: ; F: ; GC: ; M: ; N: ; NV: ; PAC: ; SS: ; V:. Plate Ultrastructrural observation of spermatogenesis in Urechis unicinctus coelom fluid 1: primary spermatocyte; 2 3: secondary spermatocyte; 4 8:spermatid; 9: sperm cell. AC: acrosome; C: centrosome; CB: intercellular bridge; ER: endoplasmic reticulum; F: flagellum; GC: Golgi complex; M: mitochodria; N: nucleus; NV: nucleus vesicle; PAC: pre-acrosome; SS: subacrosomal space; V: vesicle.

Plate Ultrastructrural observation of spermatogenesis in Urechis unicinctus coelom fluid 1: primary spermatocyte; 2 3: secondary

Microsoft Word - 15肖云朴-4校-卢冰.doc

Microsoft Word - 15肖云朴-4校-卢冰.doc 热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 2011 年第 30 卷第 1 期 :107 112 海洋生物学 http://jto.scsio.ac.cn; http://www.jto.ac.cn 鱾肖云朴 1, 徐善良 2, 孙敏 3, 吕慧明 3 (1., 325400; 2., 315211; 3., 315211)