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1 耳鲍消化酶活力的研究 1, 2, 1 (1., ; 2., ) 摘要 : 对人工养殖条件下体长 0.52~7.05cm 耳鲍 (Haliotis asinina) 的消化酶活力进行了研究结果表明, 淀粉酶活力随体长的增加而减小, 纤维素酶活力随体长的增加而递增, 脂肪酶活力随体长的增加无明显变化, 不同部位酶活力依次为 : 胃>消化腺>肠方差分析表明 : 同体长组别, 淀粉酶活力在胃肠间存在显著差异 (P<0.05), 在消化腺与肠间, 胃与消化腺间无显著差异 (P>0.05); 纤维素酶活力在胃与消化腺肠间存在显著差异 (P<0.05), 在消化腺与肠间无显著差异 (P>0.05) 关键词 : 耳鲍 (Haliotis asinina); 消化酶活力 ; 消化腺中图分类号 : Q556 文献标识码 : A 文章编号 : (2012) (Haliotis asinina),, [1] (Mollusca) (Gastropoda) (Prosobranchia) (Archaeogastropoda) (Haliotidae) (Haliotis) [2],,, 2002, ,,, [3], : (H.discus discus) [4-5], (H.discus hannai) [6-7], (H.sieboldii) [8-10], (H.gigantean) [11], (H.midae) [12-16], (H.rubra) [17], (H.laevigata) [18], (H.fulgens) [19-20]. [21-22] [23],,, 1 材料和方法 1.1 实验材料 / 2012 / 36 / 6, 7, 5, 样品处理及酶测定方法 3 cm, 1.3 cm 0~4, r/min 3, 5 min,, [24], 3,5-,,, 1.0 ml 25 ml, ml,, 5 min 1.0 ml 2mol/LNaOH 2.0 ml,, 5 min 20 ml 490 nm, 1mL : ; : : (2004AA603130); ( ); ( ); (071001) : (1978-),,, :, linguiwang@126.com;,, huangbohb1@163.com

2 表 1 不同组别耳鲍的平均体长 Tab. 1 The average body length of Haliotis asinina (cm) 0.52± ± ± ± ± ± ± µmol [24], 3, 5-, : 1.0 ml, ml, 3 min 2 ml, 5 min,, 20 ml 490 nm, 1 ml 1 ( U ) 1 mg ( ) [25],,,,,, : 5 ml ph 7.5, mol/l 4 ml, min 1mL, min, 15mL95% 2~ mol/lnaoh, NaOH, 1 ml 1 ( U ) 1 µmol 1.3 数据处理与分析 SPSS11.0, 结果与分析 2.1 不同体长耳鲍淀粉酶活力 2,,, 3 cm : , : > >, (P 0.05), (P 0.05) 表 2 不同体长耳鲍淀粉酶活力 (U/mg) Tab. 2 Activity of amylase enzyme of Haliotis asinina A B C D E F G 9.12± ± ± ±0.03 ( ) ( ) ( ) 8.75± ± ± ± ± ± ± ± ± ± ±0.05 : 1.3 cm, 2.2 不同体长耳鲍纤维素酶活力 3,, : ; : > >, (P 0.05), (P 0.05) 2.3 不同体长耳鲍脂肪酶活力 4,, : : > > 3 讨论 [23] Marine Sciences / Vol. 36, No. 6 /

3 ,,,, ( 1~ 3); ( 4~ 6), 5 cm,, 表 3 不同体长耳鲍纤维素酶活力 (U/mg) Tab. 3 Activity of cellulase enzymes of Haliotis asinine (U/mg) A B C D E F G 12.26± ± ± ±0.02 ( ) ( ) ( ) 11.07± ± ± ± ± ± ± ± ± ± ±0.02 表 4 不同体长耳鲍脂肪酶活力 (U/mg) Tab. 4 Activity of lipase enzymes of Haliotis asinine (U/mg) A B C D E F G 0.38± ± ± ±0.02 ( ) ( ) ( ) 0.32± ± ± ± ± ± ± ± ± ± ±0.02,,,,, 1 Fig. 1 Comparison on of cellulase activity in stomach among H.asinina,H.diversicolor diversicolor and H. diversicolor aquatilis [26],,,, 0.86 cm 1.20 cm, 4.46 cm 5.83 cm, 2 Fig. 2 Comparison of cellulase activity in digestive gland among H.asinina,H.diversicolor diversicolor and H. diversicolor aquatilis,,,,, 80 / 2012 / 36 / 6

4 3 Fig. 3 Comparison of cellulase activity in intestines among H. asinina, H. diversicolor diversicolor and H. diversicolor aquatilis 6 Fig. 6 Comparison of amylase activity in intestines among H.asinina, H.diversicolor diversicolor and H. diversicolor aquatilis 4 Fig. 4 Comparison of amylase activity in stomach among H.asinina, H.diversicolor diversicolor and H. diversicolor aquatilis,,,,,,, ;, 5 cm,,,,, > >, (P 0.05), : [1],,. [J]., 2002, 26(7): [2],,. [M]. :, 1998: Fig. 5 Comparison of amylase activity in digestive gland among H.asinina, H.diversicolor diversicolor and H. diversicolor aquatilis [3],,,. [C]//., 1997, 39: [4] Chamilani N, Chulhong O. Mahanama D Z. Characteriza- Marine Sciences / Vol. 36, No. 6 /

5 tion of beta-1,4-endoglucanase as a polysaccharide-degrading digestive enzyme from disk abalone, Haliotis discus discus[j]. Aquacult Int, 2010, 8: [5] Nikapitiya C, Oh C, Whang I, et al. Molecular characterization, gene expression analysis and biochemical properties of a-amylase from the disk abalone, Haliotis discus discus.[j]. Comp Biochem Physiol Part B, 2009, 152: [6] Tanaka R, Sugimura I, Sawabe T, et al. Gut microflora of abalone Haliotis discus hannai in culture changes coincident with a change in diet[j]. Fish Sci, 2003, 69: [7] Suzuki K, Ojima T, Nishita K. Purification and cdna cloning of a cellulase from abalone Haliotis discus hannai [J]. Biochem, 2003, 270: [8] Chiou T, Lai M, Shiau C. Seasonal variations of chemical constituents in the muscle and viscera of small abalone fed different diets[j]. Fish Sci, 2001, 67, [9] Hsieh Meng-shun, Yin Li-jung, Jiang Shann-tzong. Purification and characterization of the amylase from a small abalone Haliotis sieboldii[j]. Fisheries Science, 2008, 74: [10] Tsao C Y, Pan Y Z, Jiang ST. Purification and characterization of amylase from small abalone (Sulculus diversicolor aquatilis)[j]. Agric Food Chem, 2003, 51: [11] Iehata S, Inagaki T, Okunishi S, et al. Colonization and probiotic effects of lactic acid bacteria in the gut of the abalone Haliotis gigantea [J]. Fish Sci, 2009, 75: [12] Macey B M, Coyne V E. Colonization of the gastrointestinal tract of the farmed South African abalone Haliotis midae by the probionts Vibrio midae SY9, Cryptococcus sp. SS1, and Debaryomyces hansenii AY1[J]. Marine Biotech, 2006, 8: [13] Erasmus J H, Cook P A, Coyne V E. The role of bacteria in the digestion of seaweed by the abalone Haliotis midae[j]. Aquaculture, 1997, 155: [14] Sales J, Britz P J. Evaluation of the reference diet substitutionmethod for determination of apparent nutrient digestibility coefficients of feed ingredients for South African abalone (Haliotis midae L.)[J]. Aquaculture, 2001, 207, [15] Britz P J, Hecht T, Knauer J. Gastric evacuation time and digestive enzyme activity in abalone Haliotis midae fed a formulated diet[j]. South African Journal of Marine Science, 1996, 17: [16] Shipton T A, Britz P J. Evaluation of an in vitro digestibility technique for the prediction of protein digestibility in the South African abalone, Haliotis midae L[J]. Aquaculture Nutrition, 2002, 8: [17] Edwards S, Condon C, Digestive protease characterization, localization and adaptation in blacklip abalone (Haliotis rubra Leach)[J]. Aquaculture Research, 2001, 32: [18] Harris J O. A preliminary investigation of microbial impact on digestive physiology of the Greenlip abalone, Haliotis laevigata Donovan[D]. Launceston, Tasmania: University of Tasmania, [19] Hernandez-Santoyo A, Hernandez-Arana A, Arreguin-Espinosa R, et al. Purification and characterization of several digestive proteases from the blue abalone Haliotis fulgens[j]. Aquaculture, 1998, 159: [20] García-Carreño F L, Navarrete del Toro M A, Serviere-Zaragoza E. Digestive enzymes in juvenile green abalone,haliotis fulgens, fed natural food[j]. Comp Biochem Physiol, 2003, 134: [21],,. [J]., (5): 3-6. [22] Fu Jinghua, Xu wei, Mai Kangsen,et al. Effects of dietary menadione on the activity of antioxidant enzymes in abalone, Haliotis discus hannai Ino[J]. Chinese Journal of Oceanology and Limnology,2012, 30(1): [23],,. [J]., 2003, 27(2): [24]. [M].. :, 1992: [25],,. [M]. :, 1981: [26],. [J]., 1997, 21(3): / 2012 / 36 / 6

6 Studies on digestive enzymes of Haliotis asinina WANG Lin-gui 1, WANG Xiao-bin 2, HUANG Bo 1 (1. Fishery Department, Hainan University, Haikou , China; 2. College of Material Science and Chemical Engineering, Hainan University, Haikou , China) Received: Jun.,11,2011 Key words: Haliotis asinine; digestive enzyme activity; digestive gland Abstract: The digestive enzyme activities of Haliotis asinina at different stage were investigated in this paper.. The results showed that the cellulase activities increased while the amylase activities decreased with the growth of H.asinina. There were no significant changes in lipase activities. The enzymatic activity order for different parts is: stomach>digestive gland> intestine. The ANOVA analysis showed that the amylase activities exhibited significant differences (P 0.05) between the stomach and intestine at the same stage, while no significant differences (P 0.05)between the digestive gland and intestine, the digestive gland and stomach, respectively. The activities of cellulase were significantly different (P 0.05)between the stomach and digestive gland, the stomach and intestine, while not significantly different (P 0.05) between the digestive gland and intestine. ( 本文编辑 : 张培新 ) Marine Sciences / Vol. 36, No. 6 /

; H. discus hannai 19961/3 3 ; % 29% 10%<1% 5 44% 16%14% 5 90% H. d

; H. discus hannai 19961/3 3 ; % 29% 10%<1% 5 44% 16%14% 5 90% H. d 動物世界 Animal World Search for The Causes of Japanese Abalone Resource Decreasing Gwo, Jin-Chywan Department of Aquaculture, Taiwan National Ocean University, Keelung 1970 32-4 45 Mollusca GastropodaArchaeogastropoda