2015 Vol.17 No.9 Rb l Ginsenoside Rb l 20 mg/ 96.3% 实验动物 2-3 SPF SD PBS SCXK 实验试剂 Fetal Bovine Serum FBS 100 ml/

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1 乌头碱配伍人参皂苷 Rb 1 对原代培养心肌细胞能量代谢的影响 ( 成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地成都 ) 摘要 Rb 1 0.2% 0.1% Rb 1 Rb h C 0.2% C mol L mol L -1 Rb 1 0.2% Rb 1 关键词 Rb 1 doi /wst R285.5 A Superoxide Dismutase SOD [5] [1] [2] Rb 1 Rb 1 1 实验材料 [3] [4] 实验药物 Aconitine 20 mg/ Xl mol L BAI13B05) 2013ZX HCL 0.5 mol L -1 NaOH ph : World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica 1785

2 2015 Vol.17 No.9 Rb l Ginsenoside Rb l 20 mg/ 96.3% 实验动物 2-3 SPF SD PBS SCXK 实验试剂 Fetal Bovine Serum FBS 100 ml/ min min 15% FBS DMEM DMEM Dulbecco s Modified Eagle Medium 500 ml/ ml % Trypsin 250 l 5 g/ r min min Gibco % FBS DMEM Phosphate Buffer Solution PBS h 0.22 μm -20 Dimethyl Sulfoxide DMSO 500 ml/ 150 μl μl ml/ h 72 h Succinate Dehydrogenase SDH 5 / 2.2 实验分组 % 0.1% Rb 1 C Rb YAD 实验仪器 LDZ4-1.8 MCO-1SACCO 0.2% SANYO Electric Co.Ltd. Thermo MxLL-Q Rb Rb 1 Millipore SW-CJ-2F mol L -1 EsJ d= g PBS CK40-F200 OLYMPUS 0.2% 0.1% Rb 1 OPTICAL.CO.LTD Thermo Rb SHHW μl/ 2 实验方法 2.1 新生大鼠心肌细胞的原代培养 PBS PBS PBS 3 1 mm 3 0.1% min μl 参附注射液中乌头碱和人参皂苷 Rb 1 成分配伍对心肌细胞活力的影响 1 h PBS μl 10% 20 μl 5 g L -1 Methyl Thiazolyl Tetrazolium MTT 4 h ml 200 μl 1786 World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica

3 DMSO C P< nm 655 nm 2.4 人参皂苷 Rb 1 配伍乌头碱对心肌细胞能量代谢的影响 5 24 Rb PBS P<0.05 Rb 1 0.2% 0.1% Rb C Rb SDH 60 μl/ h PBS μl 6 min EP r min min P<0.05 Rb C 0.2% 4 讨论 EP 50 μl μl DMEM 24 h 表 1 人参皂苷 Rb 1 配伍乌头碱对原代培养大鼠心肌细胞活力 -20 的影响 (,n=8) SDH /% OD C Rb 1 /mol L / 实验结果 0.2% % 对心肌细胞活力的影响 ## Rb 0.2% ## P< % ## Rb % 0.2% Rb ## Rb P<0.05 P< # Rb 对心肌细胞能量代谢的影响 P< % 0.2% # ## P<0.01 表 2 人参皂苷 Rb 1 配伍乌头碱对心肌细胞能量代谢的影响 (,n=8) /% /mg g -1 Rb 1 /mol L -1 C /U L -1 /U mg -1 /ng ml # 0.2% % # Rb # Rb # Rb # # Rb # P< % # ## P<0.01 World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica 1787

4 2015 Vol.17 No.9 Rb 1 C [12] ATP [6] Rb 1 ATP C [7] Rb 1-4 [8] Rb l [13] 0.2% Rb 1 [9] 0.2% SDH [10] Rb 1 [14] SDH SDH Rb 1 [15] SDH 0.2% 0.2% Adenosine 0.2% Ttriphosphate ATP 0.2% Rb 1 SDH C ATP 0.2% Rb 1 [11] 0.2% 参考文献 1 张广平, 朱晓光, 杨海润, 等. 药性理论指导的附子配伍减毒试验研究. 世界中医药, 2014, 9(2): 李超英, 李玉梅, 张大方, 等. 附子与人参配伍对急性心衰大鼠血流动力学的影响. 中药新药与临床药理, 2011, 22(6): 张慧, 张超, 曹东东, 等. 人参对附子中枢神经系统作用影响的研究. 吉林中医药, 2012, 32(12): 汤响林, 易剑峰, 梁乾德, 等. 人参与附子配伍比例对人参皂苷成分溶出影响研究. 中草药, 2013, 44(1): 李新, 范颖. 基于析因设计探讨附子与人参黄芪干姜的交互关系. 世界科学技术 - 中医药现代化, 2013, 15(7): 彭成. 中药药理学. 北京 : 中国中医药出版社, 2012: 姜卫卫, 刘嘉. 人参皂苷 Rb 1 R g 3 R h 2 抗肿瘤作用与机制概况. 世 1788 World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica

5 界科学技术 - 中医药现代化, 2013, 15(7): Kong H L, Wang J P, Li Z Q, et al. Anti-hypoxic effct of ginsenoside Rb1 on neonatal rat cardiomyocytes is mediated through the specific activation of glucose transporter-4 ex vivo. Acta Pharmacol Sin, 2009, 30(4): 孔宏亮, 宋丽杰, 李占全, 等. 人参皂甙 Rbl 对阿霉素心力衰竭大鼠致心脏纤维化因子表达的影响. 南京医科大学学报, 2012, 32(1): 郑桃. 乌头碱与人参皂苷 Rg1 配伍对神经细胞的作用机制研究. 成都 : 成都中医药大学, 2011: 张德新, 王福元, 王瑞绵. 氟化钠对离体培养大鼠心肌细胞内糖原及琥珀酸脱氢酶的影响. 中国职业医学, 2010, 37(1): 李强, 高伟, 魏宏文. 间歇性低氧刺激对运动能力影响的实验研究. 体育科学, 2001, 21(3): 62-65, 叶鸣, 雷志平. 间歇性低氧训练在运动训练中应用中的研究进展. 中国运动医学杂, 2002, 2l(5): , Eto D, Yamano S, Mukai K, et al. Effect of high intensity training on anaerobic capacity of middle gluteal muscle in Thoroughbred horses. Res Vet Sci, 2004, 76(2): Evertsen F, Medbo J I, Jebens E, et al. Effect of training on the activity of five muscle enzymes studied on elite cross-country skiers. Acta Physiol Scand, 1999, 167(3): Compatibility Effect of Aconitine and Ginsenosides Rb 1 on Energy Metabolism of Primary Cultured Myocardial Cells Zhang Xue, Zhao Bingxiang, Song Yuqin, Yang Yuting, Dong Yanhong, Xie Xiaofang, Peng Cheng (School of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicine Resources, Sichuan Province and Ministry of Science and Technology, Chengdu , China) Abstract: This article was aimed to discuss the impact of compatibility effect of aconitine and ginsenosides Rb 1 on energy metabolism of cardiomyocytes. The trypsin was used to digest and differential adherence method was used to purify and cultivate cardiomyocytes. Rat cardiomyocytes were divided into the normal control group, 0.2% aconitine group, 0.1% aconitine group, ginsenosides Rb 1 group, compatibility of aconitine and ginsenosides Rb 1, 2 1, 1 1 ) ( 1 2 group. The drug reaction time was 1 hour. The cell vitality, glycogen content, succinate dehydrogenase, cytochrome C oxidase and LDH concentration were detected in cardiomyocytes. The results showed that 0.2% aconitine significantly reduced myocardial viability, increased the LDH concentration, reduced the glycogen content, succinate dehydrogenase and cytochrome C oxidase. The compatibility of mol L -1 or mol L -1 ginsenosides Rb 1 and aconitine was significantly against the cardiomyocytes toxicity induced by 0.2% aconitine. It was concluded that ginsenosides Rb 1 can reduce the cardiomyocytes toxicity by aconitine. Its mechanism may be associated with the cellular energy metabolism. Keywords: Ginsenosides Rb 1, aconitine, cardiomyocytes, energy metabolism World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica 1789

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