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畴桑夸戴
7 years ago
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1 / HBV DCs DCs HBV PBMCs DCs DCs HLA-DR CD80 CD86 CD83 CD1 琢 DCs IFN- 琢 IL-4 HBV HBV DCs HLA-DR CD1 琢 CD83 CD80 CD86 P<0.01 DCs IFN- 琢 P<0.01 HBV CD83 CD86 P<0.01 IL-4 P<0.01 DCs IFN- 琢 P<0.01 HBV DCs HBV doi: /wst R259 A Dendritic cells DCs DCs ~ Acute-on-Chronic Liver Fail 原 ure ACLF Chronic LiverFailure CLF ACLF 24 8 元 JDZX /H2708 DC T 2010FJ3018 HBV 元元 813

2 绎依依 6.9 B Prothrombin time activity percent 原 age PTA% P> ~ 依 ACLF CLF 3~ HCV HDV HAV HEV HGV EB DCs DCs DCs HLA -DR CD1 琢 CD80 CD86 CD83 DCs IL-4 IFN- 琢 PBS 10% Fetalcalfserum FCS Hyclone RPMI-1640 CD1a -FITC HLA -DR -P CD80 -FITC CD86 -PE CD83 -FITC IgG2b IgG1-FITC ebioscience 4 rhil-4 Peprotech rhgm-csf Pe 原 protech BD CO 2 XD Peripheral blood monouclear cells PBMCs 20 ml 50 U ml -1 PBS 10 ml 5mL 15 ml 45 毅 1cm rpm 30 min 3 PBMCs PBMCs 5 PBS 2, rpm 10 min 1mLPBS 豫 4 10% FCS RPMI-1640 PBMCs 2 伊 10 6 /ml ml 37 益 5% CO 2 37 益 RPMI ml 10% FCS RPMI

3 rhgm -CSF U ml -1 rhil U ml -1 5%CO 2 37 益 1/2 7 DCs DCs 7 DCs 1500rpm 10 min PBS rpm 10 min 2 2 伊 10 5 / 6 l.5 ml Ep 原 pendorf, 100 滋 L CD1a-FITC CD86-PE CD80-FITC CD83-FITC HLA-DR-FITC 20 滋 L 4 益 45 min PBS 2 1% 500 滋 L PE IgG CD1a HLA -DR CD80 CD86 CD83 DCs - 琢 IFN- 琢 -4 IL- 4 DCs 7 l.5 ml Eppendorf ABC-ELISA 1.3 SPSS 16.0 依 x 依 s t P< DCs DCs HLA -DR CD1 琢 CD83 CD80 CD86 P< DCs DCs IFN- 琢 P<0.01 IL DCs DCs HLA -DR CD1 琢 CD80 CD83 CD86 P<0.01 P<0.05 HLA -DR CD1 琢 CD80 CD83 CD86 P< DCs DCs IFN - 琢 P< DCs HLA-DR CD1a CD1a CD80 CD 依依依依依依依依依依 3.93 P< DCs IFN- 琢 /pg ml -1 IL-4/pg ml 依依依依 P<

4 绎 IFN- 琢 P<0.01 IL-4 P< Liver failure 60%~80% ACLF CLF [1] 90% [2] [3 4] DCs T [5] [6] DCs-T HBV [7] CTL NK DCs DCs HBV [8] [9] / HBV DCs / HBV [10] HBV DCs 淤 HBV DCs HBV DCs 于 DCs 盂 DCs IFN- 琢 DCs IL-4 HBV HBV DCs CD83 CD86 / IFN- 琢 /IL-4 HBV 3 DCs HLA-DR CD1 琢 CD83 CD80 CD 依依依依 6.1 吟依依依 2.08 绎依依 8.70 绎依依依依依依 3.93 吟绎 P<0.05 P<0.01 P< DCs 原 1 IFN- 琢 /pg ml 原 1 IL-4/pg ml 绎依绎依依依依依绎 P<0.01 P<

5 DCs IFN- 琢 IL-4 1, ,2012,5(6) 颐 321~327. 2,,, ,2008,16(10) 颐 772~775. 3,..,2008,13(1) 颐 60~61. 4,,..,2007,12(S1) 颐 6~10. 5,.., 2009,14(1) 颐 53~56. 6,,,..,2008,16(9) 颐 660~ Royer P J, Bougras G, Ebstein F, et al. Efficient monocyte-derived dendritic cell generation in patients with acute myeloid leukemia af 原 ter chemotherapy treatment: application to active immunotherapy. Exp Hematol, 2008, 36(3) 颐 329~339. 8,..,2012,28(3) 颐 169~171. 9,,,..,2011,43(1) 颐 4~6. 10,,,..,2010,20(5) 颐 260 ~262. Comparison on Dendritic Cells Function of Yang-Huang and Yin-Huang Syndrome in HBV Related Hepatic Failure Patients Zhang Tao, Ji Jing, Ji Enru, Chen Bin, Huang Yuhong (The First A ffiliated Hospital of Hunan University of Chinese Medicine / National Clinical Research Base of Traditional Chinese Medicine for Liver Disease, Changsha , China) Abstract: This study was aimed to compare the function of dendritic cells (DCs) in patients of Yin-Huang syn 鄄 drome and Y ang-huang syndrome from the peripheral blood of HBV related hepatic failure. Jaundice patients of HBV related hepatic failure were divided into two groups, which were the Yang-Huang syndrome group and Yin- Huang syndrome group. And a healthy control group was also established. The DCs were separated and cultured in vitro from PBMCs in the peripheral blood. Surface molecules such as HLA -DR, CD80, CD86, CD83 and CD1 琢 were detected by the flow cytometry. And the level of IFN- 琢 and IL-4 in the supernatant of DCs were also de 鄄 tected. The expression difference of inflammatory cytokines and immune cells between Yin-Huang and Yang- Huang syndrome in patients of HBV related hepatic failure were compared. The results showed that compared with healthy people, the expression rate of DCs phenotype such as HLA-DR, CD1 琢, CD83, CD80, CD86 in patients of HBV related hepatic failure was significantly decreased (P < 0.01); the excreted factor IFN- 琢 had a significant rising (P <0.01).ComparedtoYang-Huang syndrome group, the expression rate of CD83 and CD86 in the Yin- Huang syndrome group of HBV related hepatic failure patients was significantly reduced (P <0.01).Andtheex 鄄 creted factor of IL-4 had a significant rising in Yin-Huang syndrome group (P < 0.01). Compared to the Yin- Huang syndrome group, the excreted factor IFN- 琢 in the Yang-Huang syndrome group had a significant rising (P < 0.01). It was concluded that DCs function of patients with HBV related hepatic failure in both Yin-Huang syn 鄄 drome and Yang -Huang syndrome group showed low immune function. And the immune function of the Yin- Huang syndrome group was lower. There was excessive releasing of inflammatory factors in the Yang-Huang syn 鄄 drome group. And there was enhanced anti-inflammatory cytokine expression in the Yin-Huang group. Keywords: HBV-related liver failure, Yin-Huang syndrome, Y ang-huang syndrome, dendritic cells 817

< F63756D656E D2D796E2D31C6DABFAF2D31D6D0D2BDD2A9CFD6B4FABBAF2D C4EA2DB5DA36C6DA2DB7E2C3E6CDC6BDE9A3A D36A3A92E6D6469>

< F63756D656E D2D796E2D31C6DABFAF2D31D6D0D2BDD2A9CFD6B4FABBAF2D C4EA2DB5DA36C6DA2DB7E2C3E6CDC6BDE9A3A D36A3A92E6D6469> 1. 530023 2. 530001 3. 530001 DCP 100 DCP 47 53 3 HOMA -IR HOMA - IR P