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1 2014 绎 Vol.16 No CCl 4 SD CCl 澡 ALTAST SODGSH-PxGSH CAT MDA RT-PCR HO-1 mrna ALT AST P 约 0.05 P 约 0.01 GSH GSH-Px CAT SOD P 约 0.05 P 约 0.01 MDA P 约 0.05 P 约 0.01 HO-1 mrna P 约 0.05 P 约 0.01 CCl 4 doi: /wst R285.5 A 1 [1] 1.1 SD ~220 早 : SKSF CCl 早皂蕴 Superoxide Dismu 原 tase SODGlutathione Per 原元 EK 元元 811

2 绎 oxidase GSH -Px Glutathione GSH Catalase CAT Malondiadehyde MDA Alanine Aminotransferase ALT Aspartate Transaminase AST Adv TC-150 Sigma3K Sigma JB-600 RMBL PCR JH-163 Biometra HO -1 mrna PCR LTD PCR 茁 -actin 5' -CATCACCATCCGTAAAATGAGC -3' 5'-GACAGCCCAGGGCAGGGCATA-3' 190 bp HO -1 5' -TGGAATGCT 原 CATATAGATTGT -3' 5'-TTGAGCGAGT 原 GCTCTCTTAG-3' 270 bp 59 益 % CCl 4 3 ml kg -1 [2] g ml -1 6 伊 10-4 g ml ml 2 ml h 2.2 HE 10% 48 h HE 2.3 ALT AST Adv-156 SOD GSH-Px GSH CAT MDA ALT AST % SOD TBA MDA GSH- Px GSH CAT 2.5 RT-PCR HO-1 mrna RNA -80 益 Trizol RNA RT-PCR 淤 0.2 ml PCR RNA 3 滋 g 1 滋 g dntp 1.0 mmol RNasin 20 U AMV- RT 200 U 25 滋 L 于 PCR 95 益 1 min 益 15 s 60 益 20 s 70 益 20 s 70 益 5 min 70 益 ~ 95 益 20 s 1 益盂驻驻 CT A=CT -CT B=CT -CT =2 -K K=A-B 2.6 SPSS 13.0 x 依 s 贼择

3 2014 绎 Vol.16 No AST ALT AST ALT P 约 0.01 AST ALT P 约 0.05 P 约 0.01 AST ALT P 约 0.05 P 约 0.01 AST ALT P 约 0.05 P 约 GSH GSH-Px GSH P 约 0.01 GSH-Px P 约 0.01 GSH GSH -Px P 约 0.05 P 约 0.01 GSH GSH -Px P 约 0.05 P 约 0.01 GSH GSH-Px P 约 0.05 P 约 AST ALT x 依 s n=10 ALT/U L -1 AST/U L 依依依 ** 依 ** 依 6.17 ** 依 ** 依 4.22 ** 吟吟银银依 ** 吟银银依 4.28 ** 银依 ** 银依 * 依 * * ** P 约 0.01 吟吟吟 P 约 0.01 银银银 P 约 A B C D E F 1 HE 伊 200 A B C D E F GSH GSH-Px x 依 s n=10 GSH/mmol prot -1 GSH-Px/U mg -1 prot 依依依 ** 依 2.22 ** 依 * 依 3.18 * 依 ** 吟吟银银依 3.68 ** 吟吟银银依 ** 吟银依 3.44 ** 吟银依 * 依 2.25 * * ** P 约 0.01 吟吟吟 P 约 0.01 银银银 P 约

4 绎 3.4 CAT SOD MDA CAT SOD P 约 0.01 MDA P 约 0.01 CAT SOD MDA P 约 0.05 P 约 0.01 CAT SOD MDA P 约 0.05 P 约 0.01 CAT SOD MDA P 约 0.05 P 约 HO-1 mrna HO-1 mrna P 约 0.01 HO-1 mrna P 约 0.05 P 约 0.01 HO-1 mrna P 约 0.05 P 约 0.01 HO-1 mrna P 约 0.05 P 约 [2] [3] 40 豫 [4] [5] CCl 4 [6] CCl 4 [7] [8] [9] CCl 4 [10] ALT AST AST ALT 3 CAT SOD MDA x 依 s n=10 CAT/U g -1 SOD/U g -1 MDA/nmol g 依依依依 ** 依 ** 依 ** 依 ** 依 ** 依 ** 依 ** 吟银银依 ** 吟吟银银依 ** 吟银银依 ** 银依 ** 银依 ** 银依 * 依 * 依 * * ** 吟吟吟银银银 P 约 0.01 P 约 0.01 P 约

5 2014 绎 Vol.16 No.4 4 HO-1 mrna x 依 s n=10 HO-1 mrna 0.89 依依 0.01 ** 0.42 依 0.03 * 0.86 依 0.04 ** 吟吟银银 0.64 依 0.03 ** 吟银 0.32 依 0.02 * * ** P 约 0.01 吟吟吟 P 约 0.01 银银银 P 约 0.01 CCl 4 [11] SOD CAT CAT [12] GSH SOD MDA MDA GSH [13] 酝阅粤 SOD GSH CAT GSH-Px HO-1 [14] [15] HO-1 HO-1 LPC CCl 4 HO-1 mrna HO-1 1,,,..,2010,22(11) 颐 13~15. 2,,,.., 2009,14(1) 颐 33~35. 3,,,..2005,27(2) 颐 122~125. 4,..,2011,27(9) 颐 711~712. 5,,,..,2011,31(8) 颐 1368~ ,,,.., 2010, 21 ( 5 ) 颐 506~508. 7,,,..,2003,19(8) 颐 892~895. 8,,,. CCl 4.,2010,22(1) 颐 52~54. 9,,,. Na + -K + -ATPase Mn-SOD MDA.,2008,28 (6) 颐 ,,,..,2012,26(3) 颐 1~5. 11,,,. CCl 4.,2011,33(9) 颐 874~ 旸,,,.CCl 4.,2010,10(6) 颐 657~ ,,,.(DEHP).,2012,7(1) 颐 93~98. 14,,,. 1.,2013,17(5) 颐 945~ ,,,. HO-1.,2010,36(24) 颐 2943~2945. Effect on Softening Liver and Reducing Enzyme Mixed Agent in Prevention of CCl 4 -induced Liver Damage Rats Chen Puyan, Jiang Jinlin, Y ang Qiang (A ffiliated Minda Hospital of Hubei Institute for Nationalities, Enshi , China) Abstract: This study was aimed to observe the effect of Softening Liver and Reducing Enzyme Mixed Agent 815

6 绎 (SLREXA) in the prevention of acute liver injury rats induced by carbon tetrachloride (CCl 4 ). A total of 60 male SD rats were randomly divided into 6 groups, which were the SLREXA low-, middle-, high-dose group, glucurolactone group, normal group and model group. Intraperitoneal injection of CCl 4 was used to induce acute liver injury rat mod 鄄 el. Intragastric administration of SLREXA was given to each Chinese medicine group. Intragastric administration of distilled water was given to the normal group and the model group. Intragastric administration of glucurolactone aque 鄄 ous solution was given to the glucurolactone group. On the 12 th day of the experiment, after 16 -hour fasting, rats were killed. Pathological changes in liver tissues were examined. Blood serum was determined for alanine aminotrans 鄄 ferase (ALT) and aspartate aminotransferase (AST). The liver homogenate was determined for superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) in liver tis 鄄 sues of rats. RT-PCR was used to detect the expression level of mrna in liver heme oxygenase-1 (HO-1). The re 鄄 sults showed that in the microscopic examination of liver tissues, compared with the model group, different doses of SLREXA can alleviate pathological damages of liver in varying degrees. Levels of blood serum ALT and AST content in different doses of SLREXA groups and glucurolactone group were significantly lower than those of the model group (P <0.05orP < 0.01). Compared with the model group, contents of GSH-Px, GSH, SOD, CAT in the liver ho 鄄 mogenate were significantly increased, and MDA content was decreased significantly (P <0.05orP < 0.01) in differ 鄄 ent doses of SLREXA groups and glucurolactone group; compared with the model group, the HO-1 mrna relative expression quantity in the normal group and each treatment group increased obviously, with statistical significance (P <0.05orP < 0.01). It was concluded that SLREXA can prevent CCl 4 -induced liver injury rats with definite thera 鄄 peutic effect. Keywords: Softening Liver and Reducing Enzyme Mixed Agent, drug -induced liver injury, gene expression, traditional Chinese medicine therapy 816

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗 1338 中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用陈菲 1 2,, 艾国 3, 盛柳青 1, 麻佳蕾 1, 黄正明 1. 金华职业技术学院医学院, 浙江金华