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1 DM 8 7 (Cat ) /6/30 TEL (02) TEL (05) TEL (03) TEL (06) TEL (04) TEL (07) 免付費客服專線 01

2 ,, flow, cell viability dye cell viability dye PI, 7-AAD, Fixable Viability Stain (FVS) Reagents,FVS reagents Amine,,FVS ;, FVS, FVS 10-20, A. BD Cytofix fixation bufferm BD Perm/Wash Buffer I, B., 24,FVS 02

3 Cat. No. Description size Laser Equivalent Fluorochromes Fixable Viability Stain 440UV 200ug UV/Violet BUV Fixable Viability Stain mg Violet V450, Pacific Blue, BV Fixable Viability Stain mg Violet V500, BV Fixable Viability Stain 575V 200 µg Violet Brilliant Violet 605, Pacific Orange Fixable Viability Stain µg Blue FITC, Alexa Fluor Fixable Viability Stain µg YG/Blue PE Fixable Viability Stain µg Yellow-Green/Blue PE-CF Fixable Viability Stain µg Red APC, Alexa Fluor Fixable Viability Stain µg Red APC-R700, Alexa Fluor Fixable Viability Stain µg Red APC-Cy BD Via-Probe Red Nucleic Acid Stain 0.1 ml Red APC BD Via-Probe Red Nucleic Acid Stain 0.5 ml Red APC BD Via-Probe Green Nucleic Acid Stain 0.5 ml Blue FITC BD Via-Probe Green Nucleic Acid Stain 0.1 ml Blue FITC DAPI Solution 1 mg UV/Violet DAPI (UV)/BV421 (V) Propidium Iodide Staining Solution 2 ml YG/Blue PE 7-AAD 2 ml YG/Blue PerCP-Cy 5.5 BD Via-Probe Cell Viability Solution 500 Tests YG/Blue PerCP-Cy 5.5 BD Via-Probe Cell Viability Solution 100 Tests YG/Blue PerCP-Cy

4 , (cell proliferation) (apoptosis) Cell Proliferation ; (cell cycle),cell cycle (binary fission) ;,cell cycle : 1. (Interphase):, DNA 2. (Mitosis Phase):, cell cycle,,, / (quiescent/senescent) Gap 0 G0 ;, Gap 1 G1 ; RNA, DNA (Interphase) Synthesis S DNA Gap 2 G2 DNA, (Mitosis phase) Mitosis and cytokinesis M,,, DNA DNA Reagents Measures Mechanism Measured By hydrolyzed by intracellular nonspecific esterases to become Live cells cell proliferation dyes fluorescent products. Flow cytometry BrdU incorporation DNA Synthesis Bromodeoxyuridine replaces Flow cytometry, cell imaging thymidine (T) in dividing immunohistochemistry DNA and is then detected by Propidium iodide (PI), 7-aminoactinomycin D (7-AAD), BD Horizon Fixable DNA Content Intercalation into double stranded DNA Flow cytometry Viability Stain, Hoechst Antibodies to Ki-67, PCNA Protein level Levels increase as a result of proliferation Flow cytometry, cell imaging, immunohistochemistry, Western blot 04

Cell Proliferation Dyes CFSE & Violet Proliferation Dye 450 (VPD 450),, ester group (esterase)

Proliferation Dyes,,, muliti-color stain Staining of intracellular phenotypes on activated mouse

(protein transport inhibitor), IL-2,,VPD450 Cat. No.

5 Cell Proliferation Dyes CFSE & Violet Proliferation Dye 450 (VPD 450),, ester group (esterase) (hydrolysis), succinimidyl ester group (amino group), Cell Proliferation Dyes, BD Horizon Cell Proliferation Dyes,,, muliti-color stain Staining of intracellular phenotypes on activated mouse splenocytes CD4+ mouse splenocytes 1 µm VPD450 10, anti-cd3/cd28 4-6, PMA Ionomycin BD Golgi-Stop (protein transport inhibitor), IL-2,,VPD450 Cat. No. Description Laser Equivalent Fluorochromes* Size BD Horizon Violet Proliferation Dye 450 Violet V450, Pacific Blue, BV421 1mg BD Horizon CFSE Blue FITC, Alexa Fluor 488 1mg 05

DNA: BrdU and EdU DNA,, thymidine,bromodeoxyuridine (BrdU) S,BrdU thymidine, DNA BrdU, (Flow Cytometry) (Immunohistochemistry) BrdU-positive cell, S Cell cycle analysis of a population stained for

6 DNA: BrdU and EdU DNA,, thymidine,bromodeoxyuridine (BrdU) S,BrdU thymidine, DNA BrdU, (Flow Cytometry) (Immunohistochemistry) BrdU-positive cell, S Cell cycle analysis of a population stained for incorporated BrdU and total DNA levels (7-AAD). Human PBMC anti-cd3 anti-cd28 48, PMA Ionomycin 4, BrdU BrdU Region R3 R4 R5 R6 Cell Cycle Phase G0/G1 S G2+M Sub-G0/G1 C57BL/6,IP 1mg BrdU , (Thymus) (Bone Marrow) anti-brdu FITC 7-AAD,40 BrdU, S G0/G1, 4 BrdU+ G0/G1 Cat. No. Description Content Size FITC BrdU Flow Kit FITC-conjugated Anti-BrdU Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, BD Perm/ 50 tests FITC BrdU Flow Kit Wash Buffer (10 ), BD Cytoperm Plus Permeabilization Buffer, 7-AAD, BrdU, Dnase 200 tests APC BrdU Flow Kit APC-conjugated Anti-BrdU Antibody, BD Cytofix/Cytoperm Fixation/Permeabilization Solution, BD Perm/ 50 tests APC BrdU Flow Kit Wash Buffer (10 ), BD Cytoperm Plus Permeabilization Buffer, 7-AAD, BrdU, Dnase 200 tests BrdU In-situ Detection Kit Biotinylated Anti-BrdU Antibody, Diluent Buffer, BD Retrievagen A, Solution 1, BD Retrievagen A, 50 slides BrdU In-situ Detection Kit II Solution 2, Fixation Buffer, DAB Chromogen, DAB Buffer, Streptavidin- HRP, Control Slides 200 slides 06

Reagent (10 ) Fixative 50 test Bioimaging Proliferation Kit Solution (4% paraformaldehyde-based) Catalyst Solution DMSO EdU (5-ethynyl-2 -deoxyuridine) 6-FAM Azide 565455 (10 mm) Buffer Additive (10

7 DNA : EdU EdU (5-ethynyl-2 -deoxyuridine) thymidine, DNA, EdU S EdU BrdU,, ( ),,, BrdU EdU BrdU EdU EdU BrdU DNA Cat. No. Description Content Size Detection EdU (5-ethynyl-2 -deoxyuridine) Eterneon Red azide (10 mm) Buffer Additive (10 ) Saponin-based flow cytometry 647 EdU Click Permeabilization and Wash Reagent (10 ) Fixative 50 test Bioimaging Proliferation Kit Solution (4% paraformaldehyde-based) Catalyst Solution DMSO EdU (5-ethynyl-2 -deoxyuridine) 6-FAM Azide (10 mm) Buffer Additive (10 ) Saponin-based flow cytometry 488 EdU Click Permeabilization and Wash Reagent (10 ) Fixative 50 test Bioimaging Proliferation Kit Solution (4% paraformaldehyde-based) Catalyst Solution DMSO Equivalent Fluorochromes APC FITC Ki-67 ki-67, MKI67,, ki-67,, G0, G1 S G2,M ( ), 2011 ki-67 ki-67 14%, > 14%, ( : ) Cat.No. Description Component Detection FITC Mouse Anti-Ki-67 antibody FITC Mouse IgG1, FITC Mouse Anti-Ki-67 Set κisotype control flow cytometary PE Mouse Anti-Ki-67 antibody PE Mouse IgG1, PE Mouse Anti-Ki-67 Set κisotype control flow cytometary 07

DNA content Propidium iodide (PI) and Hoechst 33342 BD Via-Probe Nucleic Acid Stain PI DNA,PI (DNA & RNA), RNase, DNA BD Via-Probe Nucleic Acid Stain DNA nuclear counterstaining, PI 488nm, 617nm

8 DNA content Propidium iodide (PI) and Hoechst BD Via-Probe Nucleic Acid Stain PI DNA,PI (DNA & RNA), RNase, DNA BD Via-Probe Nucleic Acid Stain DNA nuclear counterstaining, PI 488nm, 617nm Hoechst Uv, 461nm Cat.No. size name Laser Equivalent Fluorochromes mg Hoechst Solution UV BUV mg DAPI Solution UV/Violet DAPI (UV)/BV421 (V) ml PI/RNase Staining Buffer YG/Blue PE ml BD Via-Probe Green Nucleic Acid Stain Blue FITC ml BD Via-Probe Green Nucleic Acid Stain Blue FITC ml BD Via-Probe Red Nucleic Acid Stain Red APC ml BD Via-Probe Red Nucleic Acid Stain Red APC ul DRAQ5 Blue PerCP-Cy ul DRAQ5 Blue PerCP-Cy ml DRAQ7 Red/YG Alexa Fluor 700/APC/PE-Cy7 :Cell cycle kit (, ) Histone DNA (Chromatin) Histone H3 15kDa,, serine 28 (S28) S10 threonine 11 S10 G2 phase anaphase ;S28 prophase anaphase Alexa Fluor 488 Mouse anti-brdu Alexa Fluor 647 Rat anti-histone H3 (ps28), Falcon 96-well Imaging Plate (Cat ) Falcon 384-well Imaging Plate (Cat ) Cat. No. name Content Size Alexa Fluor 488 Mouse anti-brdu Cell Cycle Kit Alexa Fluor 647 Rat anti-histone H3 (ps28) 5x Fixation Buffer Perm Buffer II Stain Buffer (FBS) PBS (10X) Concentrate Hoechst Solution BrdU DNase 100 tests 08

JC-1,,,JC-1 ( Aggregates ), FL2,,,,JC-1 ( Monomer ), FL1 J-aggregates (healthy cell) monomers (apoptosis indicator) JC-1(FL2) JC-1(FL1) J-aggregates (healthy cell) monomers (apoptosis indicator)

9 : (apoptosis),, (Programmed cell death, PCD),,, PS (phosphatidylserine) (depolarization) DNA, (apoptotic body) 4 1. PS Phosphatidyl-serine ( PS), Annexin V apoptosis 15, apoptosis, Wash Flow cytometry immunofluorescence, Kit : : : / 2. JC-1,,,JC-1 ( Aggregates ), FL2,,,,JC-1 ( Monomer ), FL1 J-aggregates (healthy cell) monomers (apoptosis indicator) JC-1(FL2) JC-1(FL1) J-aggregates (healthy cell) monomers (apoptosis indicator) MitoStatus reagents, (mitochondria) ; (Apoptosis) mitochondrial uncoupler (inner mitochondrial membrane potential,δψm), MitoStatus reagents, 10, MitoStatus reagents, (Apoptosis), (autophagy), (senescence). Characteristic Cat. No. Excitation peak Emission peak Laser Detector Equivalent fluorochromes* MitoStatus TMRE nm 574 nm 488 nm (blue) FL2 PE MitoStatus Red nm 648 nm 640 nm (red) FL4 APC Alexa Fluo r 647 *MitoStatus Red Annexin V FITC Apoptosis Detection Kit I (Cat. No ) ; MitoStatus TMRE Annexin V APC (Cat. No ) FVS 520(Cat. No, ) 09

10 : 3. Caspase, Caspase-3, Caspase-3 Caspase ( D4-GDI Bcl-2 ) ( PARP ) Apoptosis Jurkat cells Jurkat T cells mouse thymocytes mouse thymocytes Caspase-3 ( A, A1 ) Jurkat T cell ( B,B1 ) mouse thymocyte, ( A,B ) ( A1 ) Camptothecin 4 ( B1 ) mouse Fas ( Cat. No ), Caspase-3,, 50%, Caspase-3 4.DNA : TUNEL assay TUNEL DNA,(TdT) dutp BD Pharmingen APO-DIRECT Kit APO-Direct DNA, PI, S DNA Fragmentation, (Cell Cycle) Non-apoptotic cells Positive apoptotic cells in S phase Non-apoptotic cells Cells are labeled with both PI (DNA) and FITC-dUTP (Apoptotic Cells). Display 1:Non-clumped cells are gated. 10

550911 25slides * BD MitoScreen (JC-1) Kit 551302 100test * MitoStatus TMRE 564696 25mg * MitoStatus Red 564697 100ug * MitoStatus Caspase-3, Active Form, Apoptosis Kit

556405 60test * Apoptosis, DNA Damage, and Cell Proliferation Kit 7 Apoptosis DNA damage Cell Proliferation human mouse (488 and 635 laser), BD FACSCalibur CD3(cat.

5 PanelD:Cleaved PARP(Asp214)PE+H2AX ps139 AF647 BrdU Cat. No. Description Contents Purpose Size antibody & dye anti-brdu PerCP-Cy5.

11 PS Caspase DNA Name Cat. No. size Annexin V : FITC Apoptosis Detection Kit I test * Annexin V : PE Apoptosis Detection Kit I test * Annexin V-FITC Fluorescence Microscopy Kit slides * BD MitoScreen (JC-1) Kit test * MitoStatus TMRE mg * MitoStatus Red ug * MitoStatus Caspase-3, Active Form, Apoptosis Kit : FITC test * Caspase-3, Active Form, Apoptosis Kit:PE test * Apoptosis Detection Kit (APO-Direct) test * Apoptosis Detection Kit (APO-BRDU) test * Apoptosis, DNA Damage, and Cell Proliferation Kit 7 Apoptosis DNA damage Cell Proliferation human mouse (488 and 635 laser), BD FACSCalibur CD3(cat ) CD28(Cat ), BrdU 1 PanelA:DAPI+BrdU Per-CP-Cy5.5 PanelB: PanelA:BrdU Per-CP-Cy5.5+H2AX ps139 AF647 PanelC:Cleaved PARP(Asp214)PE+BrdU Per-CP-Cy5.5 PanelD:Cleaved PARP(Asp214)PE+H2AX ps139 AF647 BrdU Cat. No. Description Contents Purpose Size antibody & dye anti-brdu PerCP-Cy5.5 Detection of cell proliferation anti- H2AX (ps139)alexafluor 647 Detection of DNA damage Apoptosis, anti- Cleaved PARP (Asp214) PE Detection of apoptosis DAPI (optional) Determination of DNA content DNA Damage and other reagents Cell Proliferation Kit BrdU solution 50 Tests DNase BD Cytofix/Cytoperm Fixation/Permeabilization Solution BD Perm/Wash Buffer 11

12 /6/30, DM,,, 1000 * / PI *, BD Pharmingen, 2017/7/31 * 12 TEL (02) TEL (05) TEL (03) TEL (06) TEL (04) TEL (07) 免付費客服專線 U060427

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untitled 1 1-1 1-3 1-2 2 (cell membrane) (cytoplasm) (organelles) 1-1 1-1 1-1 (Cell Membrane) 1972 (S. J. Singer) (G. L. Nicholson) (fluid-mosaic model) 1. 2. 1-2 3. 1 3 (phospholipids) 55% 25% (glycoproteins)