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彰褚
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1 理例類 CNPH 行 93 年年參行 94 年 2 27

2 度率力 85 > 2.0 mg/dl 度度 HBABA [2-(4 -hydroxybenzene azo) benzoic acid] 510 nm 度 (4 g/dl) 度留力類 Piperacillin 類 Cefoperazone 量不度 (1~30 mg/dl) 3 率 (ultrafiltration membrane cone) 離離濾 HPLC 濾度度留力度 4.0 g/dl (n=47) 數 (<0.01) 度 4.0 g/dl (n=38) 數 (<0.05 piperacillin cefoperazone 率度降度 20 mg/dl (0.342 mm) 率 % 降 6.5% 率 % 降 % 率率力率率率數諸力參數量度狀率量力 (protein binding) 力狀

3 (hyperbilirubinemia)(jaundice) 塞易 serum albumin) 降率度不良療爲了度兩率異 piperacillin ( 率 20 % cefoperazone ( 率 90 % 兩不度率行 I. 料異 (> 1.0 mg/dl)-2 PiperacilliLot No.3895-T00 CefoperazonLot No.1132 Crystalline human albumi Sigma Bilirubin Sigma HBABA [2-(4 -hydroxybenzene azo) benzoic acid Sigma Centriflo, ultrafiltration membrane cone Amicon, Corp. Lexington, Mass., D2137, USA. II. LC-10AD Lichrosorb RP-18 (10 µm) 250 mm x 4 mm UV-VIS absorbance detector 冷離 Ivan Sorvall super speed RC 2-B III. 留量 () : N : 400 mg 1000 ml 2. 1 M Tris : Tris (hydroxymethyl) amino methane g

4 1000 ml. 3. : 精 120 mg HBABA 500ml 量 0.01 N 度 4C 4. (0.0003M): 精量 150 ml 500 ml, 300ml 10 ml l M Tris 450ml ph ml 量度 5. 落 : 量 10 ml 1M Tris 450ml ph ml. 6. : 7 g l00 ml 50 ml 0.9 % 理 () 100 ml 量 O.9 % 理度 4C 7. : 量 0.9 % 理 1, 2, 3, 4, 5 (g/dl) 列度 () 來 : SMA II 度 (): 4m1 (): () 料 No. of Tube Test Dye Blank Serum Blank Serum (ml) HBABA working Solution (ml) Distilled water (ml) Diluting buffer (ml) nm 度 (): A T =A T -A Dye -A Ser A T = 度 (Net Absorbance) A T : () 度 A Dye : () 度 A ser : () 度留量 : 留 4 g/dl : Reserve Binding Capacity ( % ) = A T /A s x 100 % A s : (4 g/dl) 度兩 :

5 () 1. 1/15 M Sorensen (ph=7.4) (1) 精 (KH2P04) 9.08 g 1000ml 量度 (2) 精 (Na2HPO4) 9.47 g l000ml 量度 (3) 精量 (1) 196m1 (2) 804m1 0.l N ph : () 6 3. : 精量度 (1) Piperacillin: 度 500 µg/ml (Piperacillin Na) (2) Cefoperazone: 度 500 µg/ml (Cefoperazone Na) 兩 4. -: 精量量 10 ml 2.5 m1 度 7 g/dl ph 7.4 5m1 列不度 3 () : 1. 濾 (membrane cone) 理 : 六濾濾留離 6cm SS-34 離 3000 rpm (600g) 率離 0.l % 5% 10% 精 2. 不度 4m1 濾離離 3000 rpm 率離 3. 濾 (ultrafiltrate) l ml 4 ml 量 ( 參量 ) 濾度 () 數 1. 率 (Binding rate)

6 % bound = (Y-X/Y ) x l00 X : 度 Y : 度 2. 數數 (maximum number of binding sites and association constant): Benesi-Hildebrand 數 : 1/r = 1/n + 1/nk x 1/[D] [D] 度 r : 數 n : 數 k : 數 : () : 1. (1000 µg/ml): 精 100mg l0ml 50 ml 0.025N 0.l N ph ml 量 ph 8.8 留量度量暴露 2. : (1) 度 20 mg/dl: 精量量 l0ml 2.5m1 7g/dl 1 ml 1000µg/ml ph 7.4 5m1 列不度 3 (2) 度 : 精量量 10ml 2.5m1 7g/dl 量 ph 7.4 5m1 列不度 37 () : 數度 : () : 1. : 兩了不

7 濾 2. (Internal Standard Solution): Cephradine: 精 15mg 100ml 量度精量 40ml 100ml 量度度 15µg/ml 3. : (1) 0.0lN (ph4.8): 0.2M 59 ml 0.2M 41ml 0.lN ph ml (2) 精量 (1) 650m1 750m1 350ml 250ml 0.45µm 濾濾 piperacillin cefopetrazone 4. : (1) 行 () (2) 精量濾 lml 4ml 精 0.5m1 精量 50µl (3) (ordinate) 度 (abscissa) sigma plot Y= ax + b () 濾度 : 行度 () 兩 HPLC conditions Piperacillin Cefoperazone ColumnLichrosorb RP-18 (10 mm, 250 x 4 mm) V V Eluent: MeOH/acetate buffer (0.01 N, ph 4.8) 35/65 25/75 Flow Rate (ml/min.) 1.5 ml 1.5 ml Detector: UV 254 nm V V Sensitivity (AUFS) Internal standard Cephradine Cephradine

8 濾論

9 六

10 度不度 Cefoperazone Piperacillin 力

11 論 HBABA 力留力率易度降力率 Cefoperazone 率 90% 易率臨更臨

壹、

瑩來來理不行冷度度冷麗龍來度不不度來度麗龍行來度了來來了狀異來度不來度不來老了旅了度不殺理了度度來度冷度度參 1 數量數量 600ml 250c 600ml 10 600ml 10 來 600ml 10 600ml 30ml 量 60 600ml 600ml 數 600ml 理 600ml 4 600ml