绎 1. 210023 2. 530001 6 15 10 doi: 10.11842/wst.2013.06.021 R245.3 A 2 [1] -3 Suppressor of Cytokine Signaling 3, SOCS3 [10~12] [2] 1 1 琢 Hypoxia-Inducible Factor 1 琢, HIF- 1.1 5 C57BL/6 24 1 琢 [3, 4] [5,6] [7~9] 2011A034 20 依 2 g 3~4 22 依 3 益 55 依 3 % 1 2006 1.2 2013-06-26 2013-07-19 ELISA 元 81160455 SOCS3 Insulin/Leptin 元元 1370
2013 绎 Vol.15 No.6 Mercodia Uppsala Sweden Vascular Endothelial Growth Factor, VEGF Soluble Vascular Endothelial Growth Factor Receptor 1, sflt-1 ELISA R&D systems Minneapolis US 原 A Leptin, LP ELISA Invitrogen Camarillo CA 93012NO ELISA Promega Corp USA 2 2.1 24 4 Con 原 trolcontrol + Acupuncture ObeseObese + Acupuncture 6 1 2~16 59%14% 27% 16 20% 2.2 16 1min 10 min/ 员 10 2.3 VEGF sflt-1 Leptin NO 2.4 SPSS 16.0 x 依 s One -way ANOVA LSD post-hoc test P< 0.05 3 3.1 P 约 0.05 P 跃 0.05 1 3.2 P 约 0.05 P 跃 0.05P 约 0.05 P 跃 0.05 2 3.3 VEGF sflt-1 P 跃 0.05 NO P 约 0.05 VEGF NO P 约 0.05 VEGF NO 3 3.4 P 约 0.05 P 约 0.05 1 x 依 s n=6 g 23.56 依 2.3 26.86 依 2.5 22.99 依 2.4 25.25 依 3.2 26.23 依 3.2 36.89 依 3.9 * 25.82 依 3.3 28.28 依 4.3 # * P<0.05 # P<0.05 P<0.05 6~8 10 1371
绎 4 2 x 依 s n=6 4 mg dl -1 滋 g L -1 144.21 依 3.5 142.36 依 4.2 173.65 依 5.6 179.44 依 6.1 150.25 依 4.8 148.26 依 5.2 210.25 依 7.1 * 196.88 依 6.5 * 2.44 依 0.2 2.39 依 0.3 3.67 依 0.4 3.73 依 0.3 3.45 依 0.5 2.56 依 0.2 6.95 依 1.0 * 3.88 依 0.8 # NO VEGF FGF HGF * P<0.05 # P<0.05 P<0.05 [13] NO 3 NO VEGF sflt-1 x 依 s n=6 NO 滋 mol L -1 VEGF pg ml -1 sflt-1 pg ml -1 9.25 依 0.22 9.22 依 0.32 13.22 依 1.5 11.44 依 1.8 11.23 依 0.39 10.25 依 0.4 19.56 依 2.5 * 12.33 依 0.8 # 801 依 45 798 依 67 710 依 39 728 依 46 765 依 65 968 依 89 689 依 56 1125 依 90 # 6345 依 139 6450 依 176 6800 依 131 6755 依 120 6056 依 120 7425 依 190 7325 依 160 6980 依 140 NO NO * P<0.05 # P<0.05 P<0.05 NO VEGF sflt-1 in 原 4 x 依 s n=6 pg ml -1 OS NO VEGF [14] VEGF sflt- 1 VEGFR2 sflt-1 VEGF VEGF sflt-1 [15] 912 依 102 876 依 99 2 300 依 129 * 2 356 依 143 1126 依 95 1322 依 110 6215 依 159 * 4259 依 139 *# VEGF [15] * P<0.05 # P<0.05 P<0.05 [16, 17] 86.7% 1372
2013 绎 Vol.15 No.6 NO VEGF sflt-1 NO VEGF sflt-1 [18] 1 Christiaens V, Lijnen H R. Angiogenesis and development of adi 原 pose tissue. MolCellEndocrinol, 2010, 318(1-2) 颐 2~9. 2 Liu L, Meydani M. Angiogenesis inhibitors may regulate adiposity. Nutr Rev, 2003, 61(11) 颐 384~387. 3 Wang B, Wood I S, Trayhurn P. Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in hu 原 man adipocytes. Pflugers Arch, 2007, 455(3) 颐 479~492. 4 Ye J, Gao Z, Yin J, et al. Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose tissue of ob/ob and dietary obese mice. Am J Physiol Endocrinol Metab, 2007, 293(4) 颐 E1118~E1128. 5 Halberg N, Khan T, Trujillo M E, et al. Hypoxia -inducible factor 1alpha induces fibrosis and insulin resistance in white adipose tis 原 sue. Mol Cell Biol, 2009, 29(16) 颐 4467~4483. 6 Khazaei M, Tahergorabi Z. Systemic ghrelin administration alters serum biomarkers of angiogenesis in diet-induced obese mice. Int J Pept, 2013, 2013 颐 249565. 7,,,.., 2007, 27(10) 颐 738~740. 8.., 2002, 22(2) 颐 22~23. 9. 80.,2005, 25(2) 颐 27~29. 10,. ob mrna., 2011, 17(4) 颐 423~424. 11,,,.., 2011, 6(3) 颐 235~237. 12.., 2010, 21(9) 颐 2315~2317. 13 Cao Y. Angiogenesis modulates adipogenesis and obesity. J Clin In 原 vest, 2007, 117(9) 颐 2362~2368. 14 Suganami E, Takagi H, Ohashi H, et al. Leptin stimulates ischemi 原 a-induced retinal neovascularization: possible role of vascular en 原 dothelial growth factor expressed in retinal endothelial cells. Dia 原 betes, 2004, 53(9) 颐 2443~2448. 15 Gomez -Ambrosi J, Catalan V, Rodriguez A, et al. Involvement of serum vascular endothelial growth factor family members in the de 原 velopment of obesity in mice and humans. J Nutr Biochem, 2010, 21(8) 颐 774~780. 16 Talbert M E, Langefeld C D, Ziegler J, et al. Polymorphisms near SOCS3 are associated with obesity and glucose homeostasis traits in Hispanic Americans from the Insulin Resistance Atherosclerosis Family Study. Hum Genet, 2009, 125(2) 颐 153~162. 17 Howard J K, Cave B J, Oksanen L J, et al. Enhanced leptin sensi 原 tivity and attenuation of diet-induced obesity in mice with haploin 原 sufficiency of Socs3. Nat Med, 2004, 10(7) 颐 734~738. 18.., 2012, 25(18) 颐 149~150. Influence of Specific Acupoint Acupuncture Therapy to Serum AngiogenesisFactorof Obese Mouse Models Tang Hongzhen 1, 2,ZhiLanying 2, Yan Shijun 2, Chen Dandan 2,LiuJia 2, Zhang Shaoyang 2, Dai Shen 1 (1. School of Preclinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; 2. Ruikang Hospital A ffiliated to Guangxi University of Chinese Medicine, Nanning 530001, China) Abstract: This study was aimed to discuss the influence of specific acupoint acupuncture therapy to the serum angiogenesis factor of high fat diet obese mouse models. Mice were randomly divided into the blank control 1373
绎 group, acupuncture control group, model control group, and acupuncture treatment group. There were 6 mice in each group. Obese mouse models were induced after 15-week high fat diet. The specific acupoint acupunc 鄄 ture therapy was used as an intervention treatment method for 10 days. The enzyme -linked immunosorbent as 鄄 say ( ELISA) was used in the detection of serum insulin, vascular endothelial growth factor (VEGF), soluble vascular endothelial growth factor receptor, leptin and the level of nitric oxide in mice. The results showed that the serum insulin, nitric oxide and leptin level in the obese mouse models were increased. However, there were no obvious changes on the vascular endothelial growth factor and soluble vascular endothelial growth fac 鄄 tor receptor level. The specific acupoint acupuncture therapy can obviously reduce the level of serum nitric ox 鄄 ide and leptin, and improve the content of vascular endothelial growth factor in obese mouse models. However, there were no influence on the level of blood glucose and soluble vascular endothelial growth factor receptor. It was concluded that the specific acupoint acupuncture therapy method had preferable antiobesity action. Its mechanism of action may be related to the regulation of angiogenesis. Keywords: Acupuncture, obese, angiogenesis factor 1374