Chinese Bulletin of Entomology 2010 47 3 460 ~ 466 檻檻 檻檻檻檻檻檻檻檻檻檻檻檻 * 450002 Parasitic effects on host Plutella xylostella testes by two endoparasitoids. HE Fan BAI Su-Fen LI Xin CHAO Yun-Fei College of Plant Protection Henan Agricultural University Zhengzhou 450002 China Abstract The effects of the endoparasitoids Cotesia vestalis Haliday or Diadegma semiclausum Hellén on the germ cell and testicular growth of their host Plutella xylostella L. were studied. C. vestalis and D. semiclausum carry two types of polydnaviruses. The results show that both C. vestalis bracovirus and D. semiclausum ichnovirus can inhibit host spermatogenesis and spermiogenesis there were no or only a few sperm bundles in parasitized host testes. Inhibition of spermiogenesis was almost complete in hosts parasitized during early stages of development but was less severe in those parasitized at the 4th final stadium. Testicular growth of P. xylostella was also inhibited by parasitization younger hosts developed smaller testes than those parasitized at the 4th stadium. The degree of castration therefore depends on the age at which the host is parasitized. C. vestalis had a significantly stronger suppressive effect on host germ cell and testicular growth than D. semiclausum. Key words testes endoparsitoid polydnavirus Plutella xylostella instar DNA Polydnavirus PDV 2 Plutella xylostella L. 2 PDV Bracovirus PDV Cotesia vestalis Haliday Ichvovirus PDV Diadegma semiclausum Hellén 2 DNA DNA Polydnavirus PDV Parasitic castration 1 PDV PDV * 30471168 PDV E-mail hefan601@ 163. com E-mail sfbai68@ yahoo. com. cn 2009-09-05 2010-01-15
3 461 DNA 1 500 lx PDV PDV 2 10% ~ 15% 1. 2 2 PDV 60 2 3 2 4 4 PDV 1 PDV PDV 3 2 1 2 PDV 1 3 4 Tagashira PDV 30 2 4 1 60 1. 3 5 0 1 2 3 4 5 6 7 d 5 Cotesia vestalis Haliday 1 Leica Diadegma semiclausum Hellén Canon Powershot A650 IS Plutella xylostella L. 1 ~ 4 DT2000 V2. 0 L 4 W T V = 1 / 6 4π LWT 4 2 PDV 1. 4 5 d 5 2 PDV 1 Leica Canon Powershot A650 IS 1 1. 5 1. 1 % = - 25 ± 1 60% ~ 80% L D = 14 10 1 000 ~ 4 / 100% 2
462 Chinese Bulletin of Entomology 47 4 SPSS SPSS t ANOVA 2 2 3 2. 1 4 4 1 A ~ D 1 A 2 B 3 C 4 D 4 2. 2 2 2. 2. 1 2 2 5 d 2 2 D ~ F 2 4 5 d 5 d 4 5 d 2 G ~ I 4 2 A ~ C 5 d 3 5 d 4 3 2 J ~ L
3 463 2 2 5 d A 2 5 d B 2 5 d C 2 5 d D 3 5 d E 3 5 d F 3 5 d G 4 5 d H 4 5 d I 4 5 d J 4 5 d K 4 5 d L 4 5 d 4 3 4 2. 2. 2 2
464 Chinese Bulletin of Entomology 47 3 ~ 6 2 5 d 0. 021 mm 3 71. 17% 3 A B 4 3 A B C3 3 D3 3 3 2 A B C2 2 D2 2 P < 0. 05 3 ~ 3 3 d 6 B 5 d 7 d 2 3 0. 035 mm 3 0. 028 mm 3 2 3 d 3 0. 170 mm 3 P < 0. 01 3 d 66. 19% 4 5 d 2 48. 01% 4 A B 4 4 > 4 > 3 > 2 3 ~ 6 A 2 > 3 > 4 > 4 2 3 4 P < 0. 05 2
3 465 5 4 A B C4 4 D4 4 6 4 A B C4 4 D4 4 3 Beckage 7 7 8 9 10 11 3. 1 Tanaka 12 13 Apanteles glomeratus Pieris brassicae 1 /4 10 2 14 2 2
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