110 36,,,. SBR 15,20,25,30,35,,, :(0~56d) 5min, ; (56~78d)(5h),., 4, 120min, 180min, 50min, 10min. SBR 2.5L, 50% SBR, 1, COD(N

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1 2016,36(1)109~116 China Environmental Science,,,,(,,, ), 5 (15,20,25,30 35 ).,,, Type 0041 Type 0092, 5 ;,5, SVI (15~30), 35 SVI 30.. X703.1 A (2016) Effect of temperature on activated sludge settleability and microbial community structure. WANG Jie, PENG Yong-zhen *, YANG Xiong, WANG Shu-ying, ZHANG Jian-wei (Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing , China). China Environmental Science, 2016,36(1)109~116 AbstractIn order to investigate the relationship between operating temperature and sludge settleability, five sequencing batch reactors with automatic control equipment were adopted to explore the effect of different operating temperature (15, 20, 25, 30 and 35) on sludge settleability and microbial community structure. The results showed that: when inflowing time was five minutes, sludge bulking did not occurred in all systems. This was due to that effect of operating temperature on sludge settleability was much weaker than substrate concentration, and the dominating filamentous bacterium were Type 0041 and Type 0092; when five hours were adopted, five systems were occurred filamentous bulking phenomenon and SVI value increased as operating temperature (15~30) rising due to the interaction between temperature and low substrate concentration. While operating temperature was 35, SVI value was lower than that in 30. Moreover, the features of the intracellular storage and extracellular storage as well as the types of dominating filamentous bacteria had a great difference when operating temperature was different. Key wordstemperatureactivated sludge settleabilitysludge bulkingfilamentous bulking sludgestorage [1],.,, [2].. ph,(do), (SRT),(F/M), [34] /, [5],, (EPS),,, [6]. Knoop [7] M.parvicella 5,12, (2012ZX ); *,, pyz@bjut.edu.cn

2 110 36,,,. SBR 15,20,25,30,35,,, :(0~56d) 5min, ; (56~78d)(5h),., 4, 120min, 180min, 50min, 10min. SBR 2.5L, 50% SBR, 1, COD(NaCH 2 CH 3 ) 400mg/L, C:N:P = 400:40:8, ( 1)., SVI 100mL/g SBR Fig.1 The Setup diagram of SBR system and PLC 1.; 2.; 3. ph, DO ; 4.; 5.;6. ; 7.; 8. ph, DO ; 9.; L, (SBR)( 1).SBR,,, (PLC), IKA REO basic C,, DO (2.0±0.2)mg/L. 5 15,20,25,30,35,. DO SBR (DO) PLC,PLC, DO. 78d, 1 Table 1 The composition of feedwater (g/l) (g/l) NH 4 Cl 0.15 CoCl 2 6H 2 O 0.15 K 2 HPO NaMo 4 2H 2 O 0.06 MgSO 4 7H 2 O 0.10 MnCl 2 2H 2 O 0.12 CaCl KI 0.18 NaCH 2 CO H 3 BO FeCl 3 6H 2 O 1.5 EDTA 10 CuSO 4 5H 2 O ,(SVI), (MLSS), (MLVSS),β(PHA), (EPS),PH,DO.SVI, MLSS, MLVSS [8]. PHA h, PHA,β (PHB)β(PHV),PHA PHB PHV.PHB PHV Oehmen [9], Agilent 6890N

3 1 111 Agilent DB1, 3 3( 95%: 5%, Fluka,Buchs SG, Switzerland).EPS [10], [11], Lowery [10],DNA [12], EPS 3., Olympus_ BX61. (FI) Eikelboom [13] , 5min (),,5 25 SVI 50mL/g,,,,.5 ( 3a)., 10min [14],, Chudoba [15], (μ max ), (K s ),, PHA,,, d SVI, 100mL/g, SVI, 25,,,,.,,. SVI (ml/g) (d) 2 Fig.2 Effect of different temperature on settleability of activated sludge 56d, 5 SBR, 5 5h(),.,5 SBR, 30, SVI 350mL/g,,, 5( 3e),. ( 3b~f)., SVI, 15, SVI 157mL/g,, SVI, 30 SVI 356mL/g. 35, SVI 30,SVI 300mL/g.Dilek [16], 15~30,,, 35, SVI 120mL/g,

4 , SVI. ().,,5,.,,, [16].,,,,,,,,,, 15~ 30, SVI, 35, SVI 30.,. Shin [17] /(PN/PS),,(15~35), PN/PS., Van den Eijnde [18],,,,. 3 (400x) Fig.3 Morphological observation results in different systems (a)25;(b)15;(c)20;(d)25;(e)30;(f) PHA EPS 4(a)(c) (50d); 4(b)(d)(70d). 4(a)(c), 5 PHA,,PHA PHA., PHA [3],( 35 ) PHA

5 min , PHA 15.3mmolC/ gvss 7.09mmolC/ gvss,pha. 35, PHA 15~30, 4, 15.91mmolC/ gvss, 60min. Krishna [19],, PHA.(35),,, PHA. PHA (mgcod/gvss) PHA (mgcod/gvss) min 6 24 (mmolc/gvss) (mmolc/gvss) (min) (min) 4 PHA Fig.4 The changes of PHA and glucogen in systems under different operating temperature (c) 120min,,,.,., PHA, PHA,. PHA, PHA. PHA.Mino [20] PHA,,PHA 30% (TCA) [21], PHA. 4(b)(d),, PHA,,PHA.,,,,,, ,,,

6 PHA,,,.,5 PHA. 2 EPS PN/PS Table 2 Effect of different temperature on EPS and () (50d) EPS (mg/gvss) PN/PS PN/PS ratio SVI (ml/g) (70d) EPS (mg/gvss) PN/PS SVI (ml/g) EPS PN/PS., EPS, I EPS 30mg/gVSS, II5 25mg/gVSS. EPS,,EPS, Ca 2+,Mg 2+ [22]. I, I EPS II. EPS DNA. EPS., I PN/PS II PN/PS, PN/PS,,, PN/PS EPS.Liao [23] EPS,Morgon [24] EPS EPS., EPS PN/PS. [2225],EPS,EPS SVI, EPS. EPS, Table 3 Filamentous identification results () FI Type 1701,Type 0041,Type 0092,Type 0581 Type 0581*,Type Type 0581,Type 0092, S.natans,Type Type 1701*,Type 0092,Type ~3 Type 0092,Type 0041, M.parvicella,Type 021N 0~1 Type 0041*, M.parvicella,Type 021N 3 Type 0041,Type 0092, actinomycetes,type Thiothrix.spp*,actinomycetes,Type 0581,Type Type 0092,Type 0581,Type 021N,Type S.natans*, M.parvicella,Type 0092,Type :*, [13]. 3. 3, FI 0,., Type 0041 Type 0092,. Eikelboom [13],Type 0041,Type 0092,,, Type 0041,, Type 0092,. 3,

7 1 115 FI.,.15 Type 0581, 5 Type 0581,, Eikelboom [13] Type 0581 SVI (120~130mL/g), 15,, FI=2( 5a). 20, Type 1701, Type 1701 SVI, SVI 200mL/g. Type 0092 Type 0581,,.25 Type 0041 Type 0092 ( 5c),. 30,, Thiothrix.spp,,,,FI=5( 5d).,,. Thiothrix.spp 30,. antinomycetes,,. 35 S.natans. S.natans SVI,. M.parvicella,Type 0092 Type Fig.5 Morphological observation results of five syetems in phase (a)15,g ;(b)20,n ;(c)25,g :(d)30,g ;(e)35,n 3 3.1,. 3.2, SVI. 3.3

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