92 China Biotechnology Vol. 32 No g 3 00ml 5ml 0. 5mol /L 24 % 24 ph NaOH Ca OH ml h 24h 48h 72h OD

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1 殏檪檪殏檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪 China Biotechnology 殏殏 8 Mitchell Bieniek Mitchell Bieniek 2 Garrett Flack 2 * Clostridium acetobutylicum AS. 7 Clostridium acetobutylicum AS. 32 Clostridium acetobutylicum AS. 34 Clostridium beijerinckii NCMIB 8052 NCMIB8052 Q * lvjihua@ rdfz. cn 5%

2 92 China Biotechnology Vol. 32 No g 3 00ml 5ml 0. 5mol /L 24 % 24 ph NaOH Ca OH ml h 24h 48h 72h OD 600nm h 24h 48h 72h min 5 000r /min. Clostridium acetobutylicum AS. 7 Clostridium acetobutylicum AS. 32 Clostridium acetobutylicum AS. 34 Clostridium beijerinckii NCMIB SoftMax SPECTRA MAX AJ Eppendorf 200 series Agilent Technologies HDL APPARATUS ZHWY g /L AS. 32 NCIMB NH 4 2 SO 4 2 KH 2 PO K 2 HPO 4 a d 3H 2 O NaCl MgSO 4 7H 2 O 0. 4 MnSO 4 H 2 O 24 OD 0. 0 FeSO 4 7H 2 O g /L 40 5 NH 4 2 SO 4 2 KH 2 PO K 2 HPO 4 3H 2 O NaCl MgSO 4 7H 2 O 0. 4 MnSO 4 H 2 O 0. 0 FeSO 4 7H 2 O ph g /L NH 4 2 SO 4 2 KH 2 PO K 2 HPO 4 3H 2 O NaCl MgSO 4 7H 2 O 0. 4 HPX-87H mmol /L H 2 SO ml /L OD 2a 2d MnSO 4 H 2 O 0. 0 FeSO 4 7H 2 O ABE 8

3 AS OD g /L AS AS. 32 NCIMB Fig. The change of OD and butanol concentration verses time AS. 7 AS. 32 NCMIB 8052 AS. 7 AS. 7 AS h ph NCMIB h AS h AS g /L AS. 7 2c AS. 32 NCMIB 8052 ABE AS. 7

4 94 China Biotechnology Vol. 32 No Fig2 2 The change of other substances concentration verses time 2. 2 NCMIB8052 3a AS. 32 AS. 32 AS. 7 NCMIB h OD 48h OD g / L 4 AS AS. 34 3b S AS. 34 AS. 32 NCMIB AS. 34 OD NCMIB8052 OD AS. 34 3c 3d AS. 7

5 Fig3 3 The change of OD and xylose concentration verses time AS. 34 g /L a c d AS. 32 AS. 7 NCMIB 0 ~ AS. 34 AS. 7 AS. 32 NCMIB8052 4a 4d AS. 32 NCMIB8052 NCMIB g /L AS. 7 NCMIB8052 AS. 32 NCMIB8052 NCMIB8052 AS. 34 NCMIB

6 96 China Biotechnology Vol. 32 No Fig4 4 The change of other substances concentration verses time Fig5 5 The change of sugar concentration and xylose concentration verses time NCMIB8052 AS. 32 AS. 34 5b 6 6b 24

7 b 6b AS. 34 AS g /L AS. 34 AS. 7 AS. 32 NCMIB8052 AS. 7 AS AS h AS. 7 OD 5a AS g /L AS OD AS. 34 Fig6 6 The change of other substances concentration verses time 2. 4 IMSA AS. 32 AS. 34 NCMIB8052 ph NaOH Ca OH 2 7

8 98 China Biotechnology Vol. 32 No Ca OH 2 NCMIB8052 ABE 8 NCMIB NCMIB Fig. 7 AS. 32 8g /L NCMIB8052 AS. 32 AS a NaOH 7 8b Ca OH 2 AS. 34 ABE Fig8 8 The fermentation result with hydrolysate as substrate 3 NCMIB8052 Max McGee Wu X Gu L L Shen L Z et al. Study on the developing trend of fuel ethanol and ethanol gasoline for motor vehicles. Applied Chemical Industry Mei Y F. Gansu Chemical Industry

9 Johnson J L Toth J Santiwatanakul S et al. Cultures of Clostridium acetobutylicum from Various Collections Comprise Clostridium acetobutylicum Clostridium beijerinckii and two other distinct types based on DNA-DNA reassociation. International Journal of Systematic Bacteriology Gu Y Li J Zhang L et al. Improvement of xylose utilization in Clostridium acetobutylicum via expression of the tala gene encoding transaldolase from Escherichia coli. Journal of Biotechnology Clostridium beijerinckii NCIMB strains with high concentration butanol tolerance in cassava fermentation. Liquor-Making Science &Technology Thaddeus Chukwuemeka Ezeji Nasib Qureshi Hans Peter Su H F Yang D F. Screening of Clostridium beijerinckii NCIMB Blaschek. Bioproduction of butanol from biomass from genes to bioreactors. Current Opinion in Biotechnology Isar J Rangaswamy V. Improved n-butanol production by solvent tolerant Clostridium beijerinckii. Biomass and Bioenergy Maddox I S Steiner E Hirsch S et al. The Cause of Acid Crash and Acidogenic Fermentations During the Batch Acetone-Butanol-Ethanol ABE Fermentation Process. J Mol Microbiol Biotechnol Study on the Ability of Butanol Production of Different Bacteria with the Fermentable Sugar GUO Yong-an TENG Ya-qun ZHU Ouhaodi DAU Yi-chen ZHA Jing-jing ZHU Xu ZENG Xiao XING Xiao-xue Mitchell Bieniek 2 Garrett Flack 2 LV Ji-hua The High School Affiliated to Renmin University of China Beijing China 2 Illinois Mathematics and Science Academy Illinois U. S. A Abstract With the development of the new generation of biomass energy it has become a popular topic that to use fermentable sugar and Clostridia to produce butanol. The study is going to use Clostridium acetobutylicum AS. 7 Clostridium acetobutylicum AS. 32 Clostridium acetobutylicum AS. 34 and Clostridium beijerinckii NCMIB 8052 which can produce butanol in various sources of sugar by fermentation. By comparing growth behaviors ratio of using sugars yield of butanol and by-product and endurance of butanol and xylose we are going to find out the most suitable Clostridium which can be used to the industrial. In the experiment NCMIB8052 is the most outstanding Clostridium as it has the highest yield relative high endurance and the ability using multiple sugars. Key words Biomass Butanol Fermentation Clostridium Endurance Xylose

L 8 0 g L ~0 700 g L % 6 00% 1 50% 1 3 NaCl 0 50% NH 4 2 SO % KH 2 PO % CaCO % 0 03% ~ 0 04% 2 24 h 0 02

L 8 0 g L ~0 700 g L % 6 00% 1 50% 1 3 NaCl 0 50% NH 4 2 SO % KH 2 PO % CaCO % 0 03% ~ 0 04% 2 24 h 0 02 38 5 Vol 38 No 5 2014 9 Journal of Jiangxi Normal UniversityNatural Science Sep 2014 1000-5862201405-0501-05 1 1* 2 2 1 3300222 330052 Streptomyces lincolnensis 13 8 6% 12 4% Q 93 A 0 1 2 Streptomyces