Microsoft Word 吴伟峰-

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1 6 9 Vol. 6 No Journal of Food Safety and Quality Sep., 2015 吴伟峰 1*, 张燕 1, 彭宏威 (1., ; 2.,, ) 2 摘要 :,,,, 关键词 : ; ; Research progress on sample pretreatment and rapid detection methods of zearalenone WU Wei-Feng 1*, ZHANG Yan 1, PENG Hong-Wei 2 (1. Guangdong Testing Institute for Product Quality Supervision, Foshan , China; 2. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou , China) ABSTRACT: Zearalenone is a kind of highly toxic micromolecule with weak biological estrogenic activity. Its property is relatively stable, thus, there is no specific antidote for it. There are 3 kinds of main technologies to detect zearalenone: bioassays, physicochemical detection methods and immunoassays, among which the immunoassay is more widely used due to its celerity, conciseness and delicacy. In this paper, advances on technology of sample pretreatment, rapid immunoassay detection method of zearalenone and related patents were reviewed. In addition, the technology of sample pretreatment for zearalenone and the development of its detection products were prospected. KEY WORDS: zearalenone; immunological detection; sample pretreatment 1 引言 (zearalenone, ZEN), ZEN, [1], ZEN,,,, ZEN, ZEN * 通讯作者 :,, wf_wu2009@qq.com *Corresponding author: WU Wei-Feng, Assistant Engineer, Guangdong Testing Institute for Product Quality Supervision, No. 1 East Road of Desheng, the New Town of Daliang, Foshan , China. wf_wu2009@qq.com

2 9, : 3537, ZEN, 2005 ZEN, ZEN 100 μg/kg GB ZEN 60 μg/kg [2], GB ZEN 500 μg/kg [3] ZEN (HPLC) [4] [5] [6-8] HPLC,,,,,,,, ZEN,,, ELISA HPLC 2 玉米赤霉烯酮的特性和检测方法 2.1 玉米赤霉烯酮的理化性质 F-2,, C 18 H 22 O 5, 318, 161~163, 2,4- ZEN,,,, ZEN, (zearalenol, ZEL) ZEL α β, ZEN, ZEN 3 [9,10] ZEL,, 2.2 玉米赤霉烯酮的致毒机制 ZEN,,,,, [11], 0.5 μmol/l ZEN,, [12,13] ZEN, ZEN [14] [15],,, ZEN, [16] [17], ZEN [16], CYP1A1 CYP1A1 CYP1A1, CYP1A1, 噁 3- [18] ZEN mrna, CYP1A1 [19] 2.3 玉米赤霉烯酮的主要检测技术, 3 :, 1 表 1 玉米赤霉烯酮不同检测方法的比较 Table 1 Comparison of different detection methods of zearalenone 0.01 μg/kg [20] 0.1 μg/kg, [21] 10 ng/g [22] (89 ) 3 μg/kg [23] 100 μg/kg [24] 200 μg/kg ZEN [25] 0.03 μg/kg ZEN, [26] 4 μg/kg,, [27],,,, [28]

3 3538 6,,,,, YZRM7 ZEN, ZEN, ZEN [28], Anna [29],, [25] [30] [31] ZEN,,,,,,,,,,,,,, (ELISA) (ICA),,,, ZEN,,, ZEN,,, 3 玉米赤霉烯酮的样品前处理及免疫学快速检测技术 3.1 玉米赤霉烯酮的样品前处理技术,, 0.1~10 μg/kg [20,21,32] - [33], 2 min,,,,,, 2 h,, (ELISA),,, [22] ELISA 70%, PBS, 1 ng/ml, 10 ng/g ELISA,,,,,,,, HPLC,, 1; ELISA, 2 Fig. 1 1 HPLC General process of sample pretreatment for HPLC testing Fig. 2 2 ELISA General process of sample pretreatment for ELISA testing

4 9, : 玉米赤霉烯酮的免疫学快速检测技术 (ELISA) (ICA), ELISA, ICA (GICA) 酶联免疫吸附法 (ELISA) ELISA 20 70,,, ELISA 2,, 3;,,,, 4 ELISA,,, ZEN ELISA [34], ZEN, 50 μl, 50 μl 50 μl ZEN,, 18~30 10 min;,,, 250 μl,, 2 min,, 3, 18~30 10 min;,, ZEN 21, ELISA Nuryono [23] ELISA, 200~750 μg/kg 100.2%, 3.0 μg/kg, 5~900 μg/kg 胶体金免疫层析检测法 (GICA) GICA [35],,, [36], 5, [37,38] [39] B [40] 1 GICA, ;,, ELISA ;,, GICA,,,, GICA,,, GICA GICA,,, Huang [41] McAb ZEN-BSA, ZEN, 5 min, 100 ng/ml, 3.3 玉米赤霉烯酮检测技术的相关专利,, [42] [43] [44] [45], 4,4'- ZEN,,, -CHO, ZEN-BSA, ZEN,, Fig. 3 3 The indirect competitive immunosorbent assay by immobilizing antigen

[49-51],, ZEN, ZEN,,, ZEN,,,, ZEN, ZEN, [1]. [M]. :, 2003. Wu YN. Modern food safety science [M]. Beijing: Chemical Industry Press, 2003. [2] GB 2715-2005 [S].

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Microsoft Word 马姜-出3355

Microsoft Word 马姜-出3355 6 9 Vol. 6 No. 9 2015 9 Journal of Food Safety and Quality Sep., 2015 马姜 1, 高原 1*, 王红静 2 (1.,, 100089; 2. ( ), 100080) 摘要 : 目的, 方法 HORIBA LA-950 结果,, 结论, 关键词 : ; ; ; Application of particle