286 ISSN 0255-6162 GPN 2006900031 286 93 10 20 161-2 0226531318 http://www.nlfd.gov.tw SCI Expanded 藥物 食品 化粧品檢驗分析 SCI Expanded 1
286 ( ) Lester A. Mitscher (Kansas Univ., USA) ( ) Haruki Yamada (Kitasato Institute, Japan) ( ) Kuo-Hsiung Lee (Univ. of North Carolina, USA) ( ) Ray H. Liu (Univ. of Alabama, USA) ( ) I. K. Ho (Univ. of Mississippi, USA) ( ) Peter P. Fu (NCTR, FDA, USA) ( ) Chiu S. Lin (FDA, USA) ( ) Keith Chan (Univ. of Maryland, USA) ( ) 1.937 1.143 0.597 0.533 0.506 0.418 2
286 3
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286 43 / 43 8 18.6 % 35 81.4 % 3 5 2 33 27 43 1 1 3 12 ( Listeria monocytogenes ) (Listeria spp) L. innocual. ivanoviil. welshimeril. seeligeri L. grayi (2.5 ~ 42) (ph) 1980 1981 (Coleslaw) 1983 1985 ( Mexican-style soft cheese ) 5
6 286 971990 (Listeriosis) 10 2 10 9 ( CFU/g) 25-30% ampicillinpenicillin gentamycin ) 1. 2. 2.1 100 15 15 CFU/ 2.2 2.2.1 2.2.2 2.2.3 5 ± 3 2.2.4 / 1mL 0.01 ml 5 10 ml 0.1 ml 2.2.5 90 100 mm 15 mm 2.2.6 450 ml99 ml90 ml () 2.2.7 10 100 mm13 100 mm 2.2.8 ± 1.0 2.2.9 ± 1.0 2.2.10 (Blender) (Stomacher) 2.2.11 0.45 µm 2.2.12 ( 3 mm) 2.2.13 2.2.14 : () () 2.2.15 2.2.16 2,000 g 0.1g 120 g 5 mg 2.2.17 2.2.18 ph 2.2.19 ph ph 6 8 2.2.20 2.2.21 2.2.22 1,000 2.2.23 (McFarland nephelometer standard units) 2.2.24 (Durham fermentation tube) 7 20 mm 16 150 mm 2.2.25 Staphylococcus aureus ( ATCC 49444, BCRC 14980ATCC 25923, BCRC 10781) Rhodococcus equi (ATCC 6939,BCRC 12859) Listeria monocytogenes (ATCC 19111,BCRC 14845)
7 286 Listeria innocua (ATCC 33090,BCRC 14843) 2.2.26 苷 (esculin) (lithium chloride) (nalidixic acid,sodium salt) (colistin methane sulfonate) 吖 (acriflavin)95% (bromcresol purple) (rhamnose) (xylose) (crystal violet) (ammonium oxalate) O ( safranin O 30% (sulfanilic acid) N-(1- ) [N-(1-naphthyl)ethylenediamine dihydrochloride] - (-naphthol) sodium ( ammonium) moxalactam - ( -creatine) N,N,N,N - - (N,N,N,N -tetramethyl--phenylenediamine2hcl) 3 2.2.27 2.2.27.1 (Gram stain solutions) (1) ( Hucker s ) ( ) A 2 g 95% 20 ml B 0.8 g 80 ml A B 24 (2) ( ) 2 g 1 g 5 10 1 ml 5 ml 10 ml 300 ml (3) ( ) O 2.5 g 95% 100 ml 10 ml 90 ml 2.2.27.2 3% 30% 5 ml 45 ml 2.2.27.3 ( Physiological saline solution ) 8.5 g 121 15 2.2.27.4 34 g 500 ml 1 N ph 7.2 121 15 1.25 ml 121 15 ph 7.2 0.1 2.2.27.5 0.1 M (ph 6.0) 17.6 g 500 ml ph 6.0 121 15 2.2.27.6 1% 1 g ph 6.00.1 M 100 ml 2.2.27.7 2% (2% Nalidixic acid solution ) 2.0 g 100 ml 2.2.27.8 2.5 g 20 g 500 ml 1 N ph 12.3 121 15 2.2.27.9 Moxalactam sodium (ammonium) moxalactam 1 g ph 6.00.1 M 100 ml 2 2.2.27.10 (Nitrite detection reagents) A 1 g 5 N 125 ml
8 286 B N-(1- ) 0.25 g 5 N 200 ml 2.2.27.11 (Methyl red indicator) 0.1 g 95% 300 ml 200 ml 2.2.27.12 (Voges-Proskauer reagentsvp reagents) A - 5 g 100 ml B 40 g 100 ml 2.2.27.13 酶 (Oxidase reagent) N,N,N,N - - 1 g 100 ml 2.2.28 2.2.28.1 UVM (UVM broth) 腖 (proteose peptone) 腖 (tryptone) (Lab lemco powder) (yeast extract) (NaCl) 20.0 g (KH 2 PO 4 ) 1.35 g (Na 2 HPO 4 ) 12.0 g 苷 (esculin) 1.0 g 2% (nalidixic acid) 1.0 ml 吖 (acriflavin) 12.0 mg 121 15 2.2.28.2 (Fraser broth) 腖 (proteose peptone) 腖 (tryptone) (Lab lemco powder) (yeast extract) (NaCl) 20.0 g (KH 2 PO 4 ) 1.35 g (Na 2 HPO 4 ) 12.0 g 苷 (esculin) 1.0 g 2% (nalidixic acid) 1.0 ml (lithium chloride) 3.0 g 10 ml 20 150 mm 121 15 吖 0.1 ml (2.5 mg/ml)5% (ferric ammonium citrate solution) 0.1 ml 2.2.28.3 (Modified Oxford medium, MOX) (Columbia blood agar base) 3944 g (agar) 2.0 g 苷 (esculin) 1.0 g (ferric ammonium citrate) 0.5 g (lithium chloride) 1 1% (colistin solution) 1.0 ml 121 10 ph 7.2 46 moxalactam 2 ml 12 ml 2.2.28.4 ( ) (Horse or sheep blood overlay medium, HL)
9 286 44.0 g 121 15 46 4% ( ) 46 10 ml 5 6 ml 2.2.28.5 (Trypticase soy agar, TSA) (trypticase peptone) 1 腖 (phytone peptone) (NaCl) (agar) 1 121 15 ph 7.3 0.2 2.2.28.6 (Fermentation broth) 腖 (peptone) 10.0 g (Lab lemco powder) 1.0 g (NaCl) (360 mg / 20 ml 0.1 N NaOH) 1.0 ml 900 ml ph 7.4 121 15 5 % (xylose) (mannitol) (rhamnose) 900 ml 100 ml 10 100 mm 5 ml ph 7.4 0.2 (25) 2.2.28.7 (Brain heart infusion agar, BHI agar) (calf brain infusion) 200.0 g (beef heart infusion) 250.0 g 腖 (proteose peptone) 10.0 g (NaCl) (Na 2 HPO 4 ) 2.5 g (glucose) 2.0 g 1 121 15 ph 7.4 0.2 1520 ml 1/21/4 2.2.28.8 (Brain heart infusion broth, BHI broth) (calf brain infusion) 200.0 g (beef heart infusion) 250.0 g 腖 (proteose peptone) 10.0 g (NaCl) (Na 2 HPO 4 ) 2.5 g (glucose) 2.0 g 121 15 ph 7.4 0.2
10 286 2.2.28.9 CAMP (CAMP test agar) 5% (trypticase soy agar) 8 ml 2.2.28.10 (Trypticase soy agar with 0.6% yeast extract, TSAYE) (trypticase soy agar, dehydrated) 40.0 g (yeast extract) 6.0 g 121 15 ph 7.3 ± 0.2 1520 ml 2.2.28.11 (Nitrate broth) (beef extract) 3.0 g 腖 (peptone) 1.0 g 57 ml 121 1215 ph 7.0 0.1 2.2.28.12 (Glucose broth, MR-VP broth) 腖 (proteose peptone) 7.0 g (glucose) (K 2 HPO 4 ) (agar) 1 5 ml 121 15 ph 6.9 0.2 2.2.28.13 (Glucose bromcresol purple broth) (glucose) 2.0 g 腖 (peptone) 10.0 g (beef extract) 3.0 g (NaCl) (bromcresol purple) 0.04 g 8 ml 121 15 ph 7.0 0.2 2.2.28.14 (Motility test medium, MTM) (beef extract ) 3.0 g 腖 (peptone) 10.0 g (NaCl) (agar) 4.0 g 5 ml 10 100 mm () 121 15 ph 7.4 ± 0.2 2.2.28.15 (Nutrient agar) (beef extract) 3 g 腖 (peptone) 5 g (agar) 15 g 121 15 ph 6.8 ± 0.2 1 2 2.3. 2.3.1 25 g UVM 225 ml 2 10 2.3.2
286 25 g UVM 225 ml 10 2.3.3 25 ml UVM 225 ml 10 2.3.4 (2~5 18 ) ( 45 15 ) 2.3.1 10 -l0 2.3.5 25g (ml) 2.3.1 1. (Tween 80 ) 2. 25 g (ml) 10 2.4. 2.4.1 2.3 30 2 22 0.1 ml 10 ml 35 2 26 2.4.2 苷 MOX 1/2 ( ) 35 2 24~48 24 24 24 l mm 24 20 l mm HL 35 2 18~26 (beta-hemolysis) BHI agarbhi broth 18~25 16~18 HL MOX 苷 2.4.3 2.4.3.1 2.4.2 BHI broth 鈎 (hanging drop) (wet mount) 2.4.3.2 2.4.2 BHI agar 鈎 24 (coccibacilli) (1) 16~24 (2) 1 5 (3) 1 (4) 95% (5) 30 (6) (7) 2.4.4 11
286 2.4.4.1 (Umbrella motility test) BHI agar 鈎 1/2 2025 23 35 mm 2.4.4.2 CAMP (CAMP test) Rhodococcus equi Staphylococcus aureus BHI agar R. equi S. aureus 1.0 2.0 CAMP ( ) 35 24~48 (arrow shaped hemolytical zone) S. aureus R. equi 2.4.4.3 酶 (Catalase test) 鈎 12 3% 2.4.4.4 酶 (Oxidase test) 鈎 酶 10~15 2.4.4.5 (VP test) 鈎 MR-VP 35 48 2 1 ml A 0.6 ml B 0.2 ml 4 2.4.4.6 (Methyl red test) 2.4.4.5 MR-VP 48 2 0.3 ml 2.4.4.7 (Phenol red carbohydrate utilization test) 鈎 35 48 2 24 2.4.4.8 (Glucose fermentation test) 鈎 35 48 2 2.4.4.9 (Reduction of nitrate) 鈎 35 18~24 A B 0.5~1 ml 2.5. 1. MOX + 2. 酶 + 3. ( ) + 4. 35 mm + 12
286 5.CAMP S. aureus + R. equi 6. - 7. 酶 - 8. + 9. + 10. + 11. - 12. - 13. + 14.β- + 2.6 2.3 () 1 ml () 9 ml UVM ( ) 35~37 24~ MOX () 0.1, 0.01, 0.001 ( g ml ) ( ( MPN/g MPN/mL ) 95% 95% ( ( MPN/ MPN/ ml (g) ml (g) g ml) g ml) 0.1 0.01 0.001 0.1 0.01 0.001 0 0 0 <3.6 -- 9.5 2 2 0 21 4.5 42 0 0 1 3.0 0.15 9.6 2 2 1 28 8.7 94 0 1 0 3.0 0.15 11 2 2 2 35 8.7 94 0 1 1 6.1 1.2 18 2 3 0 29 8.7 94 13
286 95% 95% ( ( MPN/ MPN/ ml (g) ml (g) g ml) g ml) 0.1 0.01 0.001 0.1 0.01 0.001 0 2 0 6.2 1.2 18 2 3 1 36 8.7 94 0 3 0 9.4 3.6 38 3 0 0 23 4.6 94 1 0 0 3.6 0.17 18 3 0 1 38 8.7 110 1 0 1 7.2 1.3 18 3 0 2 64 17 180 1 0 2 11 3.6 38 3 1 0 43 9 180 1 1 0 7.4 1.3 20 3 1 1 75 17 200 1 1 1 11 3.6 38 3 1 2 120 37 420 1 2 0 11 3.6 42 3 1 3 160 40 420 1 2 1 15 4.5 42 3 2 0 93 18 420 1 3 0 16 4.5 42 3 2 1 150 37 420 2 0 0 9.2 1.4 38 3 2 2 210 40 430 2 0 1 14 3.6 42 3 2 3 290 90 1000 2 0 2 20 4.5 42 3 3 0 240 42 1000 2 1 0 15 3.7 42 3 3 1 460 90 2000 2 1 1 20 4.5 42 3 3 2 1100 180 4100 2 1 2 27 8.7 94 3 3 3 1100 420 -- (1) ( ) 0.1, 0.01, 0.001 ( g ml) UVM MOX 3-1-0 MPN 43 43 (MPN/g MPN/mL) (2) ( ) 1, 0.1, 0.01 (g ml) 3-1-0 43 104.3 (MPN/g MPN/mL) (3) ( ) 0.01, 0.001, 0.0001 (g ml) 3-1-0 43 104.3 10 2 (MPN/g MPN/mL) 2.7 1. Johnson, J. L. 2002. Chapter 8. Isolation and identification of Listeria monocytogenes from meat, poultry and egg products. In USDA/FSIS Microbiology Laboratory Guidebook, Volume 1, 3rd ed. Revision 3, pp.8.01-8.18. Dey B. P. ed., US Government Printing Office, Washington, D.C. U.S.A. 2. Andrews, W. H., June, G.A and Sherrod, P. S. 1998. Appendix 2.Most Probable Number from Serial Dilution. In FDA Bacteriological Analytical Manual. 8th ed., pp.2.07~2.12. AOAC. International, Gaithersburg, MD, USA. 3. Hitchins, A. D. 2003. Chapter 10. Listeria monocytogenes. In FDA Bacteriological Analytical Manual. 8th. ed., pp.10.01-10.13. AOAC International, Gaithersburg, MD. U.S.A. 4. Donnelly, C. W., Brackett, R. E., Doores, S., Lee, W. H. and Lovett, J. 1998. Chapter 38. Listeria. In Compendium of methods for the microbiological examination of foods. 4th. ed., pp. 637-658. American Public Health Association. Washington, D.C. U.S.A. 14
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