activities than commercial antibacterial agents. Moreover, "Extract Cleanser was safe to organisms as the result of toxicity evaluation identified by

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圭唐
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1 Journal of China Institute of Technology Vol Assessment of Property and Function of All-Purpose Natural Anti-Bacteria Cleanser * Li-chun Wu Ying-chien Chung Sheng-shih Chang ( ) (APEO) 100 ppm 100% 1% - LD 50 APEO APEO APEO Abstract This research addresses the characteristics of extraction from natural plant. Considerations are made of antibacterial activity, toxicity, and environmental protection. "Extract Cleanser has been evaluated of various antibacterial activities in comparison with commercial products. Analysis shows that "Extract Cleanser possesses high antibacterial activity against the six tested pathogens. "Extract Cleanser in 100 ppm could completely inhibit the activity of Escherichia coli, and 1% of plant extract was for Staphylococcus aureus. Also, "Extract Cleanser was more efficient in the antibacterial 33

2 activities than commercial antibacterial agents. Moreover, "Extract Cleanser was safe to organisms as the result of toxicity evaluation identified by LD 50. Nevertheless, the Environmental Hormone test indicate that the extraction may contain low concentration of alkylphenol polyethoxylate (APEO), one class of environmental contaminants associated with the degradation of the nonionic alkylphenol polyethoxylate surfactants, which a major impediment for the future environmental licensed application. Keywords Plant Extract, Antibacterial Activity, LD 50, Environmental Hormone ABS [1] [2] 1954 Bogan Sawyer LAS NPEO [3] [4] (Environmental Hormone) [5] DDT PAE NPEO [6] (Microtox) ( ) ( ) ) [7] LD 50 LC 50 ED 50 EC 50 34

3 Journal of China Institute of Technology Vol LD 50 mole LD 50 PEG monostearate 64 ml Sorbitan monostearte 31.0 ml PEG(20) Sorbitan monolaurate 36.7 ml PEG(40) Sorbitan monostearte 60.0 ml Nonylphenol(10) 2.3 ml Octylphenol(10) 1.7 ml Alkyl benzene sulfonic acid sodium(abc) 2.0 g C 12 H 25 OSO 3 Na(SDS) 1.45 g C 17 H 35 COONa 4.1 g Alkyl hyroxyethane sulfonate 4.2 g Cetyldimethyl ammonium bromide 0.60 g Alkyl naphthalene pyridinium chloride 0.47 g Cetyltrimethyl ammonium chloride 0.87 g Dodecyl amino ethyl glycine 3.0 g Oleic acid amide 10.8 g Lauric diethanol amide 26.4 g ( ) ( ) 1. ( ) (Phyllostachys pubescens) 100% 1:1 2 NA ph (ph=7) Staphylococcus aureus (BCRC 10777) Listeria monocytogenes (BCRC 14845) Bacillus cereus (BCRC 10250) Eschericha coli (BCRC 10314) Shigella dysenteriae (BCRC 13983) Salmonella choleraesuis (BCRC 10241) 35 6 ( ) 35

4 2.pH ( ) 1. (1%) ph 5~9 ph ( ) ph 0.1M 3. ( ) 1. (10,000 ppm, 1%) ph 7.0 (20, 40, 60, 80, 100, 120 ) ( ) ,000 ppm 100 ml NA (ph=7) 35 6 ( ) 1. (NIEA B904.10B) 2. (Daphnia similis) (Cyprinus carpio) 48/96 (LC 50 ) 3. (DCB) 4. (1) 1~2 1.4~2.0 cm 300L 300 (2) ( E/m 2 /s ph 7 1 (3) 7 10% 5 mg/l (5) (6) 10% (7) % 80% 60% 40% 20% (8) 10 (9) 96 36

5 Journal of China Institute of Technology Vol (10) (11) 96 LC 50 (Graphic method) ( ) (APEO) (Alkylphenol polyethoxylates, APEOs) (Nonylphenol polyethoxylates, NPEOs) (Octylphenol polyethoxylate, OPEOs) APEOs (LC/MS) (MS/MS) (NMR) ( ) ( ) 1. ( ) ml 100 ml NB (ph=7) ,000 2,000 3,000 10,000 ppm 100 ppm 100% 1000 ppm 100% 10,000 ppm % 50.15% 91.70% % 41.00% 46.98% % 62.76% 99.99% % 87.80% 58.41% % 68.47% % % 89.60% 78.41% % 70.27% % % 93.24% 86.16% % 78.08% % % 94.10% 90.29% % 92.46% % % 97.94% 93.44% 2.4~ cfu/ml 100% 3 37

6 -1 Staphylococcus aureus (BCRC 10777)/ /G(+) -2 Listeria monocytogenes (BCRC 14845)/ /G(+) -3 Bacillus cereus (BCRC 10250)/ /G(+) -4 Eschericha coli (BCRC 10314)/ /G(-) -5 Shigella dysenteriae (BCRC 13983)/ /G(-) -6 Salmonella choleraesuis (BCRC 10241)/ /G(-) 2. ph ( ) 10,000 ppm ph ph=8 ph (ph=9) (ph=5) 3. ( ) 10,000 ppm ( ) 35 (20~120 min) (N = No e -k t ) 20 min 75% 60 min 100% 100% ( ) 20 min 20 min 68% 60 min 85% 80 min 100% ( ) 20 min 80 min ph ph 5 100% 99.69% 100% 100% 100% 100% ph 6 100% 95.69% 100% 100% 99.84% 100% ph 7 100% 92.57% 100% 100% 97.94% 93.52% ph 8 100% 36.49% 100% 100% 75.68% 100% ph 9 100% 100% 100% 100% 100% 100% 38

7 Journal of China Institute of Technology Vol Antibacterial coefficient E. coli St. aureus 20 min 40 min 60 min 80 min 100 min 120 min Contact time (min) ( ) 4. ( Triclosan) (10,000 ppm) (3% 5%) Triclosan 4 Triclosan 39

8 5 Triclosan, Triclosan 10 Triclosan % % % % % 99.83% % 99.55% % % % 97.76% % % % 99.26% % % % % 40

9 Journal of China Institute of Technology Vol % % 0 ( ) 1. (Daphnia similis) 48 LC 50 (Graphic method) mg/l (ppm) 2. (Cyprinus carpio) 96 (LC 50 ) LC mg/l (ppm) 3. LC 50 [7] LC 50 [8] (SDS) ( ) LAS( ) LAS (1~15 mg/l) LD 50 ( ) SDS LD LD (38.14/ ) [9] ph / (mg/l) ( S/cm) / / / / / / /20 41

10 [7] LC 50 SDS Cd 2+ SDS LC 50 (ppm) LC 50 (ppm) LC 50 (ppm) LC 50 (ppm) LC 50 (ppm) hr LD 50 LD 50 ( ) 2/6-4/6 ( ) ( ) ( 1 1 mg/kg <10 ppm 5 mg/kg mg ppm 5-43 mg/kg 4 cc mg ppm g/kg 1 30 gm g ,000 ppm g ,000 ppm g/kg g/kg gm gm 6 >15 g > 100,000 ppm >22.6 g/kg > 1 [9] ( ) (APEO) LC/MS LC/MS/MS NMR APEO (LC) 42

11 Journal of China Institute of Technology Vol (MS) (1.73e7/2.59e7/3.97e7) m/z (TIC) 1,000 LC/MS/MS APEO ( RT=10.86 min) MS/MS NMR APEO 6.5~7.5 ppm peak LC APEO LC APEO ( ) APEO LC NMR APEO ph % LD 50 APEO APEO [1] [2] 43

12 [3] [4] [5] [6] Crassostrea gigas [7] [8] [9]

目次前言... II 1 范围... 1 2 规范性引用文件... 1 3 术语和定义... 1 4 技术要求... 2 4.1 电器安全性要求... 2 4.2 卫生安全性要求... 2 4.3 抗菌除菌功能要求... 3 5

目次前言... II 1 范围... 1 2 规范性引用文件... 1 3 术语和定义... 1 4 技术要求... 2 4.1 电器安全性要求... 2 4.2 卫生安全性要求... 2 4.3 抗菌除菌功能要求... 3 5 yi ICS03.080.01 Y60 中华人民共和国国家标准 GB 家用和类似用途电器的抗菌除菌净化功能通则 General requirement of antibacterial and cleaning function for household and similar electrical appliances ( 报批稿 )