34 10 2012 10 Journal of Ningxia Medical University 981 1674-6309 2012 10-0981 - 05 EEFSEC 8q24 张玉荣 1,2, 王建业 3, 史晓红 2, 刘铭 2, 杨泽 2 1, 霍正浩 1. 750004 2. 100730 3. 100730 EEFSEC A 8q24 C T prostate cancer Pca 212 231 - SNP Pca 1 EEFSEC A 8q24 C T P > 0. 05 2 EEFSEC A 8q24 C T 3 SNPs 3 8q24 AA Pca P = 0. 024 SNPs Pca Gleason PSA P > 0. 05 EEFSEC A 8q24 C T 3 SNPs Pca 8q24 AA Pca EEFSEC 8q24 R737. 25 A prostate cancer Pca T 3 SNPs Pca 4 Pca 3 SNPs Pca 1 SNPs 1-2 2 Pca 50 1 Pca Pca 1. 1 Pca 212 74 ± 6 Pca 3 Pca 231 70 ± 8 PSA < 4 ng ml - 1 Pca Pca 39 EEFSEC A 8q24 C 2012-02 - 24 30972709 81061120527 BJ - 2010-30 10120101 E - mail huozhh@163. com 1. 2 DNA Bio Chain Taq DNA dntp LC - green PLUS Idaho 1. 3 PCR MJ PTC - 225 PCR HRM
982 34 / Idaho Lightscanner TMHR - I96 DNA Ep- DNA DNA DNA pendorf Biophotometer / 1. 5 Bio - Rad Gel DOC - 5 Oligo 6. 0 primer 5. 0 2000 Beckma Al- 1 ~ 2 legra TM 21R C3 1. 4 DNA EDTA 0. 5mL DNA 5-3 1 3 SNPs PCR / /bp TTGGCCTGTGTGTTACA 56 56 GGCATACGCTTTGTG AGGAGAATTGCAAACT 54 74 CCTTGATATGCAATTAGC CAAGCAGTGATCCGTTT 50 67 GCAAGTAAGTGCTAAAATAAAGGTA TTAAATTATAAAATATTTATAATATTAATTATATATATATAAATAT AATA 60 TATTATATTTATATATATATAATTAATATTATAAATATTTTATAAT TTAA GCGCGGCCGGCACTGACCCGAGACTCTGAGCGGCTGCTGGAGGTGCGGAAGCGGAGGGGCGGG 90 CCCGCCCCTCCGCTTCCGCACCTCCAGCAGCCGCTCAGAGTCTCGGGTCAGTGCCGGCCGCGC 2 3 SNPs 5-3 / /bp AGCCAGAGGATGGTAAAT 59. 2 189 GGCATACGCTTTGTG AAACGGTTGTGTACTCAC 56 316 CCTTGATATGCAATTAGC TAAGTGGAGCATAATATTGTTCTAC 59. 2 255 GCAAGTAAGTGCTAAAATAAAGGTA 1. 6 HRM MDR - SPSS 5 DNA 16. 0 OR PCR 95% 95% CI Pca P < 0. 05 5'- AGCCAGAGGATGGTAAAT - 3' 5'- GGCATACGCTTTGTG - 3' PCR 50μL DNA 5μL 10 PCR Buffer 5μL 10 mmol L - 1 dntp 1μL Taq DNA 5U μl - 1 1μL 10pmol 2 2. 1 8q24 T 8q24 T 3 CC CT TT HRM μl - 1 0. 5μL 50μL PCR 95 5 min 1 3 95 30 s 59. 2 45 s 72 5 30 s 35 72 7 min PCR HRM HRM 1. 7 Hardy - Weinberg 100% 2 3 SHEsis BLAST
10. EEFSEC 8q24 983 2. 2 EEFSEC A 8q24 EEFSEC A 8q24 C T T 3 Pca Pca Hardy - Weinberg P > P > 0. 05 0. 05 3 3 3 SNPs Pca SNPs n = 212 n = 231 P OR 95% CI A 177 0. 417 192 0. 416 0. 955 1. 008 0. 771 ~ 1. 317 C 247 0. 583 270 0. 584 AA 41 0. 193 41 0. 177 0. 825 CC 76 0. 358 80 0. 346 AC 95 0. 448 110 0. 476 HEW P 0. 765 A 300 0. 708 347 0. 751 0. 145 1. 247 0. 927 ~ 1. 678 C 124 0. 292 115 0. 249 AA 110 0. 519 130 0. 563 0. 231 CC 22 0. 104 14 0. 061 AC 80 0. 377 87 0. 377 HEW P 0. 912 C 195 0. 460 215 465 0. 871 1. 022 0. 785 ~ 1. 332 T 229 0. 540 247 0. 535 CC 45 0. 212 51 0 221 0. 976 TT 62 0. 292 67 0. 290 CT 105 0. 495 113 0. 489 HEW P 0. 797 2. 3 3 SNPs MDR 4 4 3 EEFSEC A 8q24 5 3 3 SNPs C T 3 SNPs 3 SNPs P = 0. 172 10 /10 0. 593 3 MDR EEFSEC 8q24 3 SNPs 3 EEFSEC A 8q24 C T
984 34 4 EEFSEC A 8q24 2. 4 C T 8q24 C AA Pca P P = 0. 024 SNPs Pca Gleason 0. 5253 0. 4821 3 0. 9453 9 /10 PSA 0. 5500 0. 4912 4 0. 8281 9 /10 SNP 0. 5927 0. 5464 7 0. 1719 10 /10 5 P > 0. 05 5 SNPs Pca PSA AA AC + CC P AA AC + CC P CC TC + TT P / 75 9 40 0. 461 20 29 0. 024 11 39 0. 325 < 75 15 47 37 22 9 52 Gleason 7 13 45 0. 776 33 23 0. 388 12 46 0. 162 < 7 8 32 20 20 4 36 PSA / ng ml - 1 10 12 45 0. 958 29 26 0. 576 12 44 0. 871 < 10 7 27 20 14 7 28 T 2 19 65 0. 611 43 38 0. 097 15 69 0. 487 < 2 1 6 6 1 2 5 5 19 0. 816 14 8 0. 347 3 20 0. 409 13 43 29 27 12 45 12 40 0. 800 22 28 0. 126 12 41 0. 262 12 45 33 23 8 48 20 64 0. 460 40 42 0. 165 17 67 0. 392 4 20 15 8 3 21 14 37 0. 168 5 19 0. 816 10 42 0. 732 3 9 46 13 43 9 45 21 80 0. 274 52 46 0. 399 19 82 0. 656 3 5 3 5 1 7 23 75 0. 277 49 46 0. 853 19 69 0. 342 1 10 6 5 1 10 2 4 0. 187 3 4 0. 507 3 13 0. 791 19 76 50 41 2 5 3 3 2 4 17 77 3 16 0. 571 12 6 0. 308 3 16 0. 852 20 62 40 40 15 67 1 7 3 5 2 6 23 80 0. 746 52 48 0. 924 18 85 0. 332 1 5 3 3 2 4 22 79 0. 641 52 46 0. 603 19 82 0. 767 1 6 3 4 1 6 23 84 0. 337 54 50 0. 957 20 87 0. 777 1 1 1 1 0 2 21 79 0. 182 49 48 0. 736 20 80 0. 496 3 4 4 3 0 7 SNPs 3 SNP SNP Pca SNPs
10. EEFSEC 8q24 985 SNPs 1 Jamal A Siegel R Ward E et al. 2007 Cancer statistics J. CA Cancer J Clin 2007 57 43 66. 2 Jamal A Kulldorff M Devesa SS et al. A geographic Pca analysis of prostate cancer mortality in the United States J. Int J Cancer 2002 101 2 168-174. EEFSEC A 8q24 3 Schaid DJ. The complex genetic epidemiology of prostate cancer J. Hum Mol Genet 2004 13 Spec No 1 C T 3 Pca R103-121. P > 0. 05 4 Ryo T Shusuke A Michiaki K et al. Genome - wide SNPs association study identifies five new susceptibility loci 3 SNPs for prostate cancer in the Japanese population J. Nature genetics 2010 42 9 751-754. 1. 2. 3. Pca 5. 3 J. 6 2011 17 8 682-687. 6. 11q13. 2 8q24 8q24 8q24 J. 2012 10 Pca 3 261-270. 7. 8q24 rsl3281615 7-16 8q24 14 SNP 17 Chris- J. 2011 34 1 tiane R 8q24 SNPs 73-76. Pca 18 EEFSEC 8. 8q24 SNP EEFSEC Pca J. 2007 34 13 724-727. 9 Chiara G and Matthew LF. Chromosome 8q24 Associated Cancers and MYC J. Genes & Cancer 2010 1 6 555-559. 10 Crowther - Swanepoel D Broderick P Di Bernardo MC et al. Common variants at 2q37. 3 8q24. 21 Pca 15q21. 3 and 16q24. 1 influence chronic lymphocytic leukemia risk J. Nat Genet 2010 42 132-136. Pca 11 Ghoussaini M Song H Koessler T et al. Multiple loci 8q24 C with different cancer specificities within the 8q24 gene desert J. J Natl Cancer Inst. 2008 100 962-966. AA Pca 12 Eeles RA Kote - Jarai Z Giles GG et al. Multiple P = 0. 024 Pca Gleason PSA newly identified loci associated with prostate cancer susceptibility J. Nat Genet 2008 40 316-231. P > 0. 05 13 Easton DF Pooley KA Dunning AM et al. Genome - Pca wide association study identifies novel breast cancer - - susceptibility loci J. Nature 2007 447 EEFSEC 1087-1093. A 8q24 C T 14 Turnbull C Ahmed S Morrison J et al. Genome - Pca 8q24 wide association study identifies five new breast cancer C AA Pca susceptibility loci J. Nat Genet 2010 42 504-507. P = 0. 024 Pca ( 下转第 1008 页 )
1008 34 difference of serum LHBs in different HBV - DNA level groups F = 23. 90 had statistically significant P < 0. 01. Serum LHBs showed direct correlation with HBV - DNA level r = 0. 78 P < 0. 01. The LHBs positive patients' ALT AST markers were higher than those in the HBV - LP negative patients. Conclusion LH- Bs showed a high coincidence rate with HBV - DNA. Monitoring serum LHBs is a good adding test for diagnosing HBV replication and active. Key words hepatitis B virus hepatitis B virus large surface protein hepatitis B virus DNA ( 上接第 985 页 ) 15 Thomas G Jacobs KB Yeager M et al. Multiple loci identified in a genome - wide association study of prostate cancer J. Nat Genet 2008 40 310-315. 16 Eeles RA Kote - Jarai Z Al Olama AA et al. Identification of seven new prostate cancer susceptibility loci through a genome - wide association study J. Nat Genet 2009 41 1116-1121. 17 Ahmadiyeh N Pomerantz MM Grisanzio C et al. 8q24 prostate breast and colon cancer risk loci show tissue - specific long - range interaction with MYC J. Proc Natl Acad Sci U S A. 2010 107 9742-9746. 18 Christiana R Jada BT Stanley H et al. Confirmation study of prostate cancer risk variants at 8q24 in African Americans identifies a novel risk locus J. Cold Spring Harbor Laboratory Press 2007 17 1717-1722. The Association between EEFSEC 8q24 and Prostate Cancer in Chinese Han Populations ZHANG Yu - rong 1 2 WANG Jian - ye 3 SHI Xiao - hong 2 LIU Ming 2 YANG Ze 2 HUO Zheng - hao 1 1. Basic Medical Academy of Ningxia Med. Univ. Yinchuan 750004 2. Dept. of Beijing Hospital Key State Laboratory of Ministry of Health Beijing 100730 3. Urology of Beijing Hospital Beijing 100730 Abstract Objective To explore the associations between 3 SNPs at EEFSEC and at 8q24 and Pca and the relative clinical risk factors in Chinese Han populations. Methods Blood samples and personal information of 212 Pca patients and 231 controls were collected. The frequencies of alleles and genotypes of these 3 SNPs in two groups were analyzed based on the model of case - control and case - only studies. We also explored the association between these loci and the clinical characteristics. Results 1 There is no significantly different distribution of risk alleles and genotype frequencies in cases and controls among the association of EEFSEC A and 8q24 C T and Pca P > 0. 05. 2 The interaction of 3 SNPs in EEFSEC A and 8q24 C T showed that EEFSEC and 8q24 had synergism. 3 The interaction between genes and the environment people with genotype AA on the site of had less probability to get Pca when getting older P = 0. 024. Other sites had no significant relevance P < 0. 05 with the age of Pca on - set PSA level Gleason and pathology on indicators. Conclusion Combined EEFSEC A and 8q24 C T might raise the risk of Pca of Chinese Han people. People with genotype AA on the site of have less probability to get Pca when getting older. Key words gene EEFSEC 8q24 prostate cancer