AFLP % 1. 1 DNA 5 RAPD DNA 0. 1g % ~ ph 6 10 DNA 9 40μl TE SSR STS 20ng /L

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忙车唐
9 years ago
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1 Journal of Plant Genetic Resources / SSR 21 SSR ~ Shannon ~ % ~ % SSR Genetic Diversity of Peanut Arachis hypogaea L. Varieties Bred in Hebei Shandong and Henan Provinces GENG Jian LIU Li-feng CUI Shun-li CHEN Huan-ying WEI Xin-yan Key Laboratory of Crop Germplasm Resources of Hebei Province / College of Agronomy Agricultural University of Hebei Baoding Abstract In order to assess the genetic diversity 41 peanut varieties bred in Hebei Henan and Shandong provinces were used with methods of SSR markers phenotypic identification and cluster analysis. Using 21 pairs of SSR primers 41 peanut varieties were screened and 52 alleles were detected with 2-4 alleles per locus and average of Shannon information index of varieties ranged from to with an average was for each pair of primers. Cluster analysis based on SSR markers showed that 3 groups and 7 subclasses were clustered while threshold was The coefficient of variation for nine kinds of agronomic traits were at range of % %. And the result of cluster analysis based on agronomic traits showed that these varieties were divided into 6 categories and 11 subclasses when threshold was Varieties in each category have their own characters. Key words Peanut Varieties SSR Genetic diversity Arachis hypogaea L. 48% 56% SSR ~ D C xtreegj@ hotmail. com liulifeng@ hebau. edu. cn

2 AFLP % 1. 1 DNA 5 RAPD DNA 0. 1g % ~ ph 6 10 DNA 9 40μl TE SSR STS 20ng /L SSR SSR μl PCR 40ng DNA 1 PCR Buffer 0. 5U Taq 4mM dntp min 95 30s SSR 1min min min 5% 80W 40min Sanguinetti 10 1 Table 1 The peanut varieties Code Name Code Name Code Name Shannon 11 I = - P i lnp i 1 0 P i i SSR DPS Version / 100%

3 m 0. 4m % 5 2 Table 2 Performance and variance of agronomical characters 2 % Trait Max Min Mean s V R CV cm Stem length Total of branch g Biomass / Plant No. of three pods Total of pods g Pod mass /Plant Hollow pod number / Plant g 100-pod weight g 100-seed weight % Ⅱ Ⅰ 8% Ⅲ % Ⅱ 18 3 Ⅳ / 5 Ⅲ % Ⅳ 4 1 Ⅴ Ⅴ Ⅵ % % % % Ⅵ % 22% Ⅰ

4 Fig. 1 1 Dendrogram of cluster analysis based on agronomic trait 2. 2 SSR AFLP % 2 ~ AFLP Fig. 2 2 PMc SSR The pattern of 41 cultivars amplified by PMc SSR SSR 21 SSR Shannon Shannon ~ SSR 4 Shannon SSR SSR I II III II I RAPD

5 Table 3 SSR 41 Shannon SSR SSR Markers Numbers of alleles and Shannon information index for each SSR marker No. of alleles Shannon I Shannon information index PM PM PM PMc PMc PM PM PM PM PM S PM PM PM PM PM PM PM PM PM PM Table 4 Genetic diversity of varieties bred in different provinces 3. 2 Shannon I No. Shannon information index No. of alleles Hebei Henan Shandong Total DNA Shannon Nei' Jiang 23 Tang SSR 21 8 Shannon Nei'

6 SSR J J RAPD J DNA J J SSR J Guillemaut P Drouard L M. Isolation of plant DNA A fast inexpensive and reliable method J. J Plant Mol Bio Rep Sanguinetti C J Dias Neto E Simpson A J. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels J. Biotechniques Williams J G K Kubelik A R Livak K J et al. DNA Polymerphisms amplified by arbimary primers are useful as genetic morkors J. Nucl Acids Res DPS M M RAPD J J J AFLP J SSR SSR J Fig. 3 Dendrogram analysis based on SSR J Chen G Du X M. Genetic diversity of source germplasm of upland SSR cotton in China as determined by SSR Marker analysis J. Acta 21 SSR 41 Genet Sinica J Shannon RAPD J Jiang H F Liao B S Ren X P et al. Comparative assessment of genetic diversity of Peanut Arachis hypogaea L. genotypes with various levels of resistance to bacterialwilt through SSR and AFLP analyses J. J Genet Genom M Tang R H Gao G Q He L Q et al. Genetic diversity in cultivatedgroundnut based on SSR markers J. J Genet Genom M

7期xin

7期xin 中国农学通报 0,0():- Chinese Agricultural Science Bulletin 花椰菜种质资源遗传多样性 SRAP 标记分析荆赞革, 唐征, 裴徐梨, 刘庆, 张小玲, 罗天宽, 朱世杨 ( 浙南作物育种重点实验室, 温州科技职业学院, 浙江温州 00; 作物遗传