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1 2016 3, 23(2): Journal of Fishery Sciences of China 研究论文 DOI: /SP.J 郑小淼 1, 李小勤 1, 2, 3, 4, 魏静 1, 胡静 1, 孙文通 1, 徐怀兵 1, 冷向军 1, 2, 3, 4 1., ; 2., ; 3., ; 4., (ZF1206), 摘要 : ( ) ( ) 75% ( ) 60% ( ) 60% ( ) (59.0±0.6) g (Ctenopharyngodon idella), 8, (P<0.05), (P<0.05);, 56.9%, 30.6% 21.2%(P<0.05);, (P<0.05); (TAA) (DAA) (P<0.05),, (SOD) (P<0.05), (MDA) (CK) (P<0.05), (MDA) (P<0.05);, (P<0.05), α- (P<0.05), (P<0.05),, ;, : ; ; ; ; ; ; ; 中图分类号 : S96 文献标志码 : A 文章编号 : (2016) (Vicia faba L.) (Ctenopharyngodon idella), [1],,, [2],, : [3 4],, [5] (G6PD) [6],, [7 8],, G6PD (GSH) ATP, GSH,, [9], 收稿日期 : ; 修订日期 : 基金项目 : (13ZR ). 作者简介 : (1989 ),,,. xmzheng @163.com 通信作者 :,,. xjleng@shou.edu.cn

2 2 : 371 [10], (Vicia sativa L.) [11] (Momordica charantia) [12],, ( ) 8,, : ; 75%, ( DL- 49%) [13], (broad bean diet, BBD); 60%, (common vetch seed diet, CVD) (bitter melon seed diet, BMD)(, 75%,,,,,, 60%);, 24 h,, (soaked broad bean, SBB),,, ;,, ( 1) 40 ( 20 ),, ( KL 105A-4-2 ) 2 mm, , 1, 375, ( 59.0 g±0.6 g) 15 (2.5 m 1.2 m 1.2 m), 5, 3 ( ), (5.0 m 3.0 m 1.2 m), 25 3 % 3 (09:00, 13:00, 17:00),, 15 min ( 1 h ), 1/3 ; 0.85~0.90 m, 20~30, DO> 4.5 mg/l, <0.2 mg/l, , 24 h,, (WGR) (FCR) (SR); 6,,,, (HSI) (VSI) (CF) (%)=100 [ (g) (g)]/ (g); = (g)/[ (g) (g)]; Tab. 1 表 1 蚕豆大巢柴籽及苦瓜籽常规成分 Proximate composition of broad bean, common vetch seed and bitter melon seed x ±SD item broad bean common vetch seed bitter melon seed moisture 12.5± ± ±0.2 crude protein 29.2± ± ±0.2 crude lipid 3.5± ± ±0.5 ash 3.5± ± ±0.1

3 Tab. 2 表 2 饲料组成及营养水平 Composition and nutrient levels of diets %, at fed basis item control diet SBB BBD CVD BMD fish meal soybean meal cottonseed meal rapeseed meal wheat bran rice bran wheat middling wheat flour dry broad bean common vetch seed bitter melon seed soybean oil choline chloride Ca(H 2 PO 4 ) ) vitamin premix 1) ) mineral premix 2) micro-encapsulated Met total nutrient level moisture crude protein crude lipid ash : 1) : V A 6000 IU, V C 200 mg, V D 2000 IU, V E 50 mg, V K 5 mg, V B1 15 mg, V B2 15 mg, V B3 30 mg, V B5 35 mg, V B6 6 mg, 0.2 mg, 3 mg, V B mg, 200 mg. 2) : Zn 80 mg, Fe 150 mg, Cu 4 mg, Mn 20 mg, I 0.4 mg, Co 0.1 mg, Se 0.1 mg, Mg 100 mg. Note: 1) The vitamin premix provided the following per kg of diets: retinyl acetate A 6000 IU, ascorbic acid 200 mg, cholecalciferol 2000 IU, tocopherol 50 mg, menadione 5 mg, thiamin 15 mg, riboflavin 15 mg, niacin 30 mg, pantothenicacid 35 mg, pyridoxamine 6 mg, biotin 0.2 mg, folic acid 3 mg, cobalamin 0.03 mg, inositol 200 mg. 2) The mineral premix provided the following per kg of diets: zinc 80 mg, iron 150 mg, copper 4 mg, manganese 20 mg, iodine 0.4 mg, cobalt 0.1mg, selenium 0.1 mg, magnesium 100 mg. (%)=100 ( )/ ( ); (%)=100 (g)/ (g); (%)=100 (g)/ (g); (g cm 3 )=100 (g)/ 3 (cm 3 );, 1.3.2, 6, (0.1% ), 3000 r/min 5 min, 80, (AST) (ALT) M(IgM) (ALP) ALT AST, IgM (ELISA) ( ); ALP - ( ), BS-200,, ( ) 80

4 2 : 373,, ( / =1/9) 4 min, 4 10 min (2500 r/min),, α-, (SOD) (MDA) (prot), 1 ml 40 ph 10.5, 1 min 1 µg 1 (U), 37, 1 g 1 min, 1 µmol/l 1 (U); α-, 1 mg min, 10 mg 1 (U); SOD ; MDA ; ( ) 1.3.3, ; ; ; 550 ; ( ),,, 11.1 [14] 6 1 g ( 3 ), 60, 50 mg, 6 mol/l h,, Sykam S-433D (, ),, 80, ( / =1/9) 4 min, 4 10 min(2500 r/min), (CK) (prot) ; ( ) 1.4 ± ( x SD ), SPSS 17.0 ANOVA, P<0.05 Duncan s , Tab. 3 表 3 蚕豆大巢菜籽及苦瓜籽饲料对草鱼生长指标和形体指标的影响 Effects of BBD, CVD and BMD diets on growth and physical indicators of grass carp n=3; x ±SD group item control SBB BBD CVD BMD /g IBW 59.0± ± ± ± ±0.4 /g FBW 126.3±0.6 a 91.0±1.8 c 109.1±1.9 b 81.6±0.4 d 84.2±1.1 d /g FI 2.28±0.03 a 1.78±0.11 c 1.95±0.04 b 1.11±0.02 e 1.43±0.04 d /% WGR 114.2±1.4 a 54.3±3.0 c 85.2±5.9 b 37.7±1.6 d 42.8±2.1 d FCR 2.00±0.12 a 3.29±0.33 d 2.40±0.09 b 3.08±0.04 c 3.51±0.25 d /% SR /% HSI 2.40±0.39 a 2.25±0.40 ab 1.88±0.40 cd 2.10±0.39 bc 1.81±0.38 d /% VSI 9.43±0.87 a 9.74±1.29 a 8.18±1.20 b 8.33±1.43 b 7.55±0.67 b /(g cm 3 ) CF 1.98± ± ± ± ±0.06 : (P>0.05), (P<0.05). Note: In the same row, values with no letter or the same superscripts mean no significant difference (P>0.05), while with different superscripts mean significant difference (P<0.05).

5 (P<0.05), (P<0.05), 56.9%, (P<0.05), 27.1% (P<0.05), 30.6% 21.2% (P<0.05) (P>0.05), (P<0.05), (P>0.05),, (P>0.05) 2.2 4, ALT (P<0.05),, AST (P>0.05), ALT (P>0.05) AST (P<0.05) ALP (P<0.05),, ALP (P<0.05), IgM (P>0.05) Tab. 4 表 4 蚕豆大巢菜籽及苦瓜籽饲料对草鱼血清肝胰脏及肌肉生化指标的影响 Effects of BBD, CVD and BMD diets on biochemical indicators in serum, hepatopancreas and muscle of grass carp n=3; x ±SD group item control SBB BBD CVD BMD ALT/(U L 1 ) 5.1±1.7 a 8.8±2.9 bc 9.5±1.7 c 6.0±1.3 ab 4.7±0.8 a AST/(U L 1 ) 34.6±5.7 a 35.3±5.8 a 36.0±3.3 a 29.7±0.9 a 20.2±4.1 b ALP/(U L 1 ) 22.1±2.9 a 13.4±2.6 b 14.6±2.2 b 13.9±3.1 b 9.7±1.5 c IgM/(mg ml 1 ) 0.78± ± ± ± ±0.09 SOD/(U mg 1 prot) 161.1±2.1 c 92.3±7.4 a 100.9±8.1 a 134.9±3.9 b 208.4±10.2 d MDA/(nmol mg 1 prot) 1.63±0.19 a 2.54±0.23 c 2.11±0.42 b 2.62±0.32 c 1.27±0.15 a CK/(U mg 1 prot) 41.8±0.4 a 55.7±4.6 b 60.5±1.0 b 61.8±3.8 b 40.0±8.4 a : (P>0.05), (P<0.05). Note: In the same row, values with no letter or the same superscripts mean no significant difference (P>0.05), while with different superscripts mean significant difference (P<0.05)., SOD (P<0.05), MDA (P<0.05), SOD (P<0.05), MDA (P>0.05), MDA (P<0.05) 2.3 5,, (P<0.05), α- (P<0.05); (P<0.05), (P>0.05) 2.4 6,, (P<0.05), (P<0.05); (P<0.05), (P>0.05);, 29.6% 29.6% 33.3% 44.4%(P<0.05) (P>0.05) 2.5 7, Asp Glu Val Ile Phe TAA DAA

6 2 : 375 Tab. 5 item 表 5 蚕豆巢菜籽及苦瓜籽饲料对草鱼前肠消化酶的影响 Effects of BBD, CVD and BMD diets on intestinal digestive enzymes of grass carp group n=3; x ±SD control SBB BBD CVD BMD /(U mg 1 prot) protease 22.1±2.5 a 14.1±2.7 b 16.9±3.5 b 16.7±0.7 b 25.0±2.0 a /(U g 1 prot) lipidase 12.9±1.5 a 7.1±1.3 b 6.9±1.9 b 5.9±0.8 b 5.8±0.8 b α- /(U g 1 prot) amylase 18.9±5.6 a 31.7±1.9 c 31.3±1.3 c 23.8±4.1 b 32.4±2.0 c : (P>0.05), (P<0.05). Note: In the same row, values with no letter or the same superscripts mean no significant difference (P>0.05), while with different superscripts mean significant difference (P<0.05). Tab. 6 表 6 蚕豆巢菜籽及苦瓜籽饲料对草鱼肌肉成分的影响 Effects of BBD, CVD and BMD diets on muscle composition of grass carp %, wet basis; n=3; x ±SD group item control SBB BBD CVD BMD moisture 76.96±0.52 a 78.88±0.34 c 78.00±0.27 b 78.60±0.33 c 78.96±0.18 c crude protein 19.94±0.25 a 18.78±0.37 c 19.88±0.59 a 19.28±0.26 b 18.87±0.16 c crude lipid 2.04±0.37 a 1.41±0.17 bc 1.58±0.17 b 1.35±0.10 bc 1.32±0.20 c ash 1.47± ± ± ± ±0.12 collagen 0.27±0.09 a 0.35±0.06 b 0.35±0.03 b 0.36±0.01 b 0.39±0.06 b : (P>0.05), (P<0.05). Note: In the same row, values with no letter or the same superscripts mean no significant difference (P>0.05), while with different superscripts mean significant difference (P<0.05). Tab. 7 表 7 蚕豆巢菜籽及苦瓜籽饲料对草鱼肌肉氨基酸组成的影响 Effects of BBD, CVD and BMD diets on muscle amino acid composition of grass carp %, dry basis; n=3; x ±SD group item control SBB BBD CVD BMD Asp* 8.98±0.01 a 8.96±0.00 a 9.06±0.11 a 9.29±0.20 ab 9.40±0.14 b Thr** 3.83± ± ± ± ±0.02 Ser 3.61± ± ± ± ±0.00 Glu* 12.79±0.40 a 13.08±0.18 a 13.66±0.69 ab 13.87±0.59 ab 14.73±0.21 b Gly* 4.33±0.20 b 4.05±0.10 a 4.42±0.02 b 4.49±0.11 b 4.42±0.07 b Ala* 5.28± ± ± ± ±0.08 Val** 4.13±0.12 a 4.12±0.06 a 4.19±0.06 a 4.36±0.10 ab 4.51±0.13 b Ile** 3.76±0.10 ab 3.70±0.01 a 3.73±0.01 a 3.92±0.01 bc 3.99±0.15 c Leu** 7.40± ± ± ± ±0.12 Tyr 2.76± ± ± ± ±0.12 Phe** 3.68±0.08 ab 3.64±0.02 a 3.70±0.06 ab 3.82±0.06 bc 3.90±0.06 c His 3.68±0.04 a 3.78±0.01 a 3.28±0.17 b 3.62±0.13 a 3.53±0.15 ab Lys** 8.13± ± ± ± ±0.17 Arg 4.83±0.07 a 4.89±0.00 ab 4.91±0.09 ab 5.05±0.15 ab 5.12±0.03 b Pro 2.15± ± ± ± ±0.03 TAA 79.29±1.34 a 79.57±0.72 a 79.82±0.13 ab 81.82±0.57 bc 83.08±1.23 c EAA 30.90± ± ± ± ±0.65 DAA 31.37±0.65 a 31.35±0.34 a 32.31±0.72 ab 32.96±0.67 b 33.78±0.50 b : * ; **. (P>0.05), (P<0.05). Note: * delicious amino acids. **means essential amino acids for human. In the same row, values with no letter or the same superscripts mean no significant difference (P>0.05), while with different small letter superscripts mean significant difference (P<0.05).

7 (P<0.05), TAA DAA, His (P<0.05) Gly (P<0.05), Thr Ser Ala Leu Tyr Lys Pro EAA (P>0.05) 3 3.1, [15 18],,,, ( 6.68 g/kg [10] 7.13 g/kg [11] 5.24 g/kg [12] ) 10 g/kg,, [9], [7 8] ; Vilarino [19] 10.1 g/kg,,, [20],,,, [17, 21],,,, 75%,,,,, 60%,,, α-, α-,,, 75% 60%,,,,,,, ;,, 36%~37% [13, 22] [23] 80% 20,, (860 g),,,, 20%, 13.0%, 7.1% [24],,, [12] [25] [26] α- [27],,,, [27] 3.2 [28], ALT AST ALP,,, ALT, [17] [23] ;,

8 2 : 377 AST, Teschke [29] AST, 60%, 40%, AST ALP, ALP [30],, ALP, ( 37.9 mg/kg, 13.3 mg/kg [31] );, 80 mg/kg,, [32], Azaza [13] 0~36% 4, 0.52% 1.97% ALP, 4- (4-hydroxynonenal) [33 34], [35], [36],, MDA, SOD,,, Mager [7], β-, G6PD (GSH), ;, MDA, 3,, 1.78 g 1.46 g,,, ;, AST MDA, SOD, [26] α- [27] (CK) ADP, [37], ATP,,, 3.3 [38], [24, 39 40], [15 17, 21],, Azaza [13] Gaber [22], 36%~37%,,,, ( 5),,,,, :,,, [39, 41 42], [43 44], [45], [15 16, 18, 21, 41] :,, I [46], [47],, [40] [23]

9 378 23,,, ( ),,,, 4,, ;, 参考文献 : [1] Lin W L, Zeng Q X, Zhu Z W. Different changes in mastication between crisp grass carp (Ctenopharyngodon idellus C. et V) and grass carp (Ctenopharyngodon idellus) after heating: The relationship between texture and ultrastructure in muscle tissue[j]. Food Res Int, 2009, 42(2): [2] Tan Q K, Li H S. Effects of diets dose and water quality on the transformation of common grass carp (Ctenopharyngodon idellus C. et V) to crisped grass carp[j]. Acta Ecologica Sinica, 2006, 26(7): [,. (Ctenopharyngodon idellus C. et V) [J]., 2006, 26(7): ] [3] Jiao L M, Yuan W. Anti-nutritional factors in broad bean[j]. Machinery for Cereals Oil and Food Processing, 2004(2): [,. [J]., 2004(2): ] [4] Li X Q, Qiu A Y. Advances of physiologically beneficial substances from broad bean[j]. Cereals & Oils, 2002(7): [,. [J]., 2002(7): ] [5] Kuang X M, Zhang H, Chen B, et al. Comparison on the content of the nutrient composition in grass carp s muscle and the Glucose-6-Phosphodehydrogenase in its erythrocyte from the beginning to the end of making grass carp s meat crisp to the taste[j]. Natural Science Journal of Hainan University, 2004, 22(3): [,,,. -6- [J]. :, 2004, 22(3): ] [6] Tan Q K, Li H S. Preliminary study on the ecology and pathology of crisped grass carp (Ctenopharyngodon idellus C. et V)[J]. Acta Ecologica Sinica, 2006, 26(8): [,. (Ctenopharyngodon idellus C. et V) [J]., 2006, 26(8): ] [7] Mager J, Glaser G, Razin A, et al. Metabolic effects of pyrimidines derived from fava bean glycosides on human erythrocytes deficient in glucose-6-phosphate dehydrogenase[j]. Biochem Biophys Res Commun, 1965, 20(2): [8] Sartori E. On the pathogenesis of favism[j]. J Med Genet, 1971, 8(4): [9] Muduuli D S, Marquardt R R, Guenter W. Effect of dietary vicine and vitamin E supplementation on the productive performance of growing and laying chickens[j]. Brit J Nutr, 1982, 47(1): [10] Wanga P X, Ueberscharb K H. The estimation of vicine, convicine and condensed tannins in 22 varieties of fababeans(vicia faba L.)[J]. Anim Feed Sci Technol, 1990, 31(1 2): [11] Farran M T, Darwish A H, Uwayjan M G, et al. Vicine and convicine in common vetch (Vicia sativa) seeds enhance beta-cyanoalanine toxicity in male broiler chicks[j]. Int J Toxicol, 2002, 21(3): [12] Zhang H X, Wang Y W, Zhang X Y, et al. Analysis of vicine in bitter melon with high performance liquid chromatography[j]. Analyt Lett, 2003, 36(8): [13] Azaza M S, Wassim K, Mensi F, et al. Evaluation of faba beans(vicia faba L. var. minuta) as a replacement for soybean meal in practical diets of juvenile Nile tilapia Oreochromis niloticus[j]. Aquaculture, 2009, 287 (1 2): [14] Shen T, Wang J Y. Biochemistry (2nd edition)[m]. Beijing: Higher Education Press, 1990: [,. : 2 [M]. :, 1990: ] [15] Li B S, Leng X J, Li X Q, et al. Effects of feeding broad bean on growth and flesh quality of grass carp Ctenopharyngodon idellus[j]. Journal of Fishery Sciences of China, 2008, 15(6): [,,,. [J]., 2008, 15(6): ] [16] Lun F, Leng X J, Li X Q, et al. Effect of feeding broad bean on growth and flesh quality of grass carp (Ctenopharyngodon idellus)[j]. Freshwater Fisheries, 2008, 38(3): [,,,. [J]., 2008, 38(3): ] [17] Li Z M, Leng X J, Li X Q, et al. Differential analysis of

10 2 : 379 growth performance, muscle quality, serum biochemical indicators and digestive enzymes of crisped grass carp[j]. Jiangsu Agricultural Sciences, 2012, 40(3): [,,,. [J]., 2012, 40(3): ] [18] Qin Z Q. Effects of feeding different raw material on growth and flesh quality of grass carp (Ctenopharyngodon idellus) [J]. Journal of Fujian Fisheries, 2010(1): [. [J]., 2010(1): ] [19] Vilarino M, Metayer J P, Crepon K, et al. Effects of varying vicine, convicine and tannin contents of fababean seeds(vicia faba L.)on nutritional values for broiler chicken[j]. Anim Feed Sci Technol, 2009, 150(1 2): [20] Francis G, Makkar H P, Becker K. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish[j]. Aquaculture, 2001, 199(3 4): [21] Li B S, Leng X J, Li X Q, et al. Effect of feeding broad bean on growth, muscle composition and intestine protease activity of different sizes of grass carp, Ctenopharyngodon idellus[j]. Journal of Shanghai Fisheries University, 2008, 17(3): [,,,., [J]., 2008, 17(3): ] [22] Gaber M M. Partial and complete replacement of fish meal by broad bean meal in feeds for Nile tilapia, Oreochromis niloticus, L., fry[j]. Aqu Res, 2006, 37(10): [23] Mao P, Hu Y, Xun Z L, et al. Effects of feeding broad bean and dehulling broad bean diets on growth performance, muscle quality and blood physiological-biochemical indices of grass carp(ctenopharyngodon idellus)[j]. Chinese Journal of Animal Nutrition, 2014, 26(3): [,,,., [J]., 2014, 26(3): ] [24] Yalsin S, Tuncer I, Yalsin S, et al. The use of different levels of common vetch seed (Vicia sativa L.) in diets for fattening rabbits[j]. Livest Prod Sci, 2003, 84(1): [25] Wong R C H, Fong W P, Ng T B. Multiple trypsin inhibitors from Momordica cochinchinensis seeds, the Chinese drug Mubiezhi[J]. Peptides, 2004, 25(2): [26] Xiao F G, Sun J T, Wang D G. Study on extraction and antioxidant activity of flavonoids from bitter melon seed[j]. Food Research and Development, 2014, 35(10): [,,. [J]., 2014, 35(10): ] [27] Liu S B, Wu M H, Huang W H, et al. Preparation of α- momorcharins from Momordica charantia and study on biological activity[j]. West China Journal of Pharmaceutical Sciences, 2010, 25(6): [,,,. α- [J]., 2010, 25(6): ] [28] Benjamin M N. Outline of Veterinary Clinical Pathology[M]. Iowa: Iowa University Press, 1978: [29] Teschke R. Bereits alcoholbedingte fettleber behandeln?[j]. Klinikarzt, 1983, 12: [30] Lumeng L, Li T K. Characterization of the pyridoxal 5 -phosphate and pyridoxamine 5 -phosphate hydrolase activity in rat liver. Identity with alkaline phosphatase[j]. J Biol Chem, 1975, 250(20): [31] Liu M, Tan H Z, Tian X H, et al. Correlation analysis of mineral content and cooking quality from 20 broad bean varieties[j]. Spectroscopy and Spectral Analysis, 2010, 30(8): [,,,. 20 [J]., 2010, 30(8): ] [32] Kumar V, Sinha A K, Makkar H P S, et al. Phytate and phytase in fish nutrition[j]. J Anim Physiol Anim Nutr, 2012, 96(3): [33] Melin A M, Perromat A, Deleris G. Pharmacologic application of fourier transform IR spectroscopy: in vivo toxicity of carbon tetrachloride on rat liver[j]. Biopolymers, 2000, 57(3): [34] Michiels C, Raes M, Toussaint O, et al. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress[j]. Free Radic Biol Med, 1994, 17(3): [35] Szymonik L, Czechowska G, Stryjecka Z M, et al. Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication[j]. J Hepato-Biliary-Pancreatic Surg, 2003, 10(4): [36] Cigermis Y, Turel H, Adiguzel K, et al. The effects of acute acetaminophen toxicity on hepatic mrna expression of SOD, CAT, GSH-Px, and levels of peroxynitrite, nitric oxide, reduced glutathione, and malondialdehyde in rabbit[j]. Mol Cell Biochem, 2014, 391(1 2): 257. [37] Yamashita K, Yoshioka T. Activities of creatine kinase isoenzymes in single skeletal muscle fibres of trained and untrained rats[j]. Pflugers Arch, 1992, 421(2): [38] Benoit F, Helene A D, Michel L, et al. Growth and meat quality relations in carp[j]. Aquaculture, 1995, 129(1 4):

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12 2 : 381 Effects of broad bean, common vetch seed and bitter melon seed diets on the growth, flesh composition, tissue indices and digestive enzymes of grass carp ZHENG Xiaomiao 1, LI Xiaoqin 1, 2, 3, 4, WEI Jing 1, HU Jing 1, SUN Wentong 1, XU Huaibing 1 1, 2, 3, 4, LENG Xiangjun 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai , China; 2. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Aquaculture; Shanghai Ocean University, Shanghai , China; 3. Shanghai Engineering Research Center of Aquaculture, Shanghai , China; 4. Shanghai University Knowledge Service Platform, Aquatic Animal Breeding Center (ZF1206), Shanghai Ocean University, Shanghai , China Abstract: Five diets, including complete formula diet (control), soaked broad bean (SBB, fed as crumbled pieces after immersionin water), broad bean, common vetch seed and bitter melon seed diets (BBD, CVD, BMD), which included 75% broad bean, 60% common vetch seed and 60% bitter melon seed in formula diet, respectively, were fed to juvenile grass carps with an initial body weight of (59.0±0.6) g. After 8 weeks of feeding, the control fish had the highest weight gain (WG) and lowest feed conversation ratio (FCR) among all groups (P<0.05). Compared with the SBB group, the WG was 56.9% in the BBD group, and 30.6% and 21.2% in the CVD and BMD groups (P<0.05), respectively. Muscle collagen contents of the SBB, BBD, CVD and BMD groups were significantly higher, while muscle lipid contents were significantly lower than those of the control (P<0.05). The BMD group had higher total amino acids and delicious amino acids contents in muscle than those of the control and SBB groups (P<0.05). The hepatopancreatic superoxide dismutase (SOD) activities of fish fed the BBD, CVD and SBB diets were significantly lower (P<0.05), and hepatopancreatic malonaldehyde (MDA) content and muscle creatine kinase (CK) activity were higher than those of fish fed the control diet (P<0.05). However, fish fed the BBD diet had lower hepatopancreatic MDA than fish fed the SBB diet. Fish fed the BBD, CVD, BMD and SBB diets had a lower lipidase activities and a higher amylase activities in their intestines compared with the control fish (P<0.05). The fish fed the BBD, CVD and SBB diets also showed lower protease activities than the control (P<0.05). In conclusion, soaked broad bean and high levels of dietary broad bean, common vetch seeds and bitter melon seed saltered the muscle composition of grass carp, and significantly decreased their growth performance. For growth and muscle composition, a broad bean diet has more advantages in the culture of crisped grass carp than common vetch seed, bitter melon seed and soaked broad bean diets. Key words: grass carp; broad bean; common vetch seed; bitter melon seed; growth performance; biochemical indices; muscle composition; digestive enzyme Corresponding author: LENG Xiangjun. xjleng@shou.edu.cn

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗 1338 中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月九味肝泰胶囊对高脂饮食诱导大鼠非酒精性脂肪肝的治疗作用陈菲 1 2,, 艾国 3, 盛柳青 1, 麻佳蕾 1, 黄正明 1. 金华职业技术学院医学院, 浙江金华