Microsoft Word 李小佳-二校出3114

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1 7 8 Vol. 7 No Journal of Food Safety and Quality Aug., 2016 李小佳, 赵志红 *, 杨扬, 朱慧 (, ) 摘要 :,,,,, ;,, 关键词 : ; ; Research progress on detection of fatty acids in food by gas chromatography LI Xiao-Jia, ZHAO Zhi-Hong *, YANG Yang, ZHU Hui (Hangzhou Wahaha Group Co., Ltd., Hangzhou , China) ABSTRACT: Fatty acids are the important components of food and one of main sources of the body's energy. It is of great help to create a reasonable diet plan by acquiring the content of fatty acids. As food safety incidents have frequently occurred in recent years, determination of fatty acids in food has become the focus of the food safety work. Gas chromatography is still the most common detection method for fatty acids in food. The national standard detection methods of fatty acids in food and recent progress on gas chromatography in fatty acids at home and abroad were summarized in this paper, including the application of gas chromatography on different kinds of food, optimization of the pre-treatment methods, as well as the tandem mass spectrometry methods. Finally, the future trends of detection methods for fatty acids in food were also prospected. KEY WORDS: food; fatty acid; gas chromatography 1 引言 (fatty acid),,,,,, (SCFA, 4~6 C), (MCFA, 8~12 C) (LCFA, >12 C), (SFA, ) (MUFA, 基金项目 : ( ) (2015C37074) Fund: Supported by Public Welfare Technology Applied Research Projects in Zhejiang Province (2015C37074) * 通讯作者 :,, zzhxuan@sina.com *Corresponding author: ZHAO Zhi-Hong, Senior Engineer, Hangzhou Wahaha Group Co., Ltd., Hangzhou , China. zzhxuan@sina.com

2 8, : ) (PUFA, 2 2 ) [1],, (C14:0) (C12:0),,, ;,, (C4:0),, ; (C16:0), [2], (EPA) (DHA), [3,4],, (TFA),,,,,,,,, 2 相关检测方法, [5] (GC), (HPLC) [6,7] - (HPLC-MS/MS) [8,9] [10,11] [12] 1 [13-19] 2.1 不同检测物质的应用 FID, 反式脂肪酸分析,, [20], (GC-FID), %~10.75%, C18:1t, 46.59%~80.65% [21] 8, HP ~1.26 mg/kg, 70%~110%Tyburczy [22] 25, (ATR-FTIR) GC-FID, 2%, ATR-FTIR, [23] ,12-2,, [24] , TFAs, Table 1 表 1 食品中脂肪酸检测方法的标准 The standards of detection methods for fatty acids in foods SN/T EPA DHA mg/g GB/T EPA DHA mg/kg GB/T %( ) SN/T %( ) GB mg/kg GB ~10 mg/kg GB %

3 3116 7, TFAs [25] KOH- GC-FID 5, 0.4%~1.1%( ),, 0 g/100 g 常规脂肪酸分析,,,, DHA EPA, [26] - KOH-, JH-FFAP, 6, ( ),,,, [27], 1% -,,, [28] 7,, KOH- 90%,, ;,,, [29], 12, 8, 62.3%, 2 3,, [30], min, - (GC-MS/MS),, [31] Agilent HP-88 GC-FID, 99.59%, 2.60%, [32], ARA EPA DHA,, 95.4%~104.7% [33] -, (DHA) (AA),,, DHA AA [34] EPA DHA,, 96.54%~101.0%, (GC 20 min ), EPA DHA [35] DB-5MS, GC-FID 11 DHA EPA, 2.2 检测方法的优化,,, 脂肪提取的优化,,, [36] Florisil,, 5, GC sn-2 sn-2, sn-2 [37] (ASE), 125, kpa, : : (3:2:1, V:V:V), C16:1-t9, 96.1%~99.3%, 0.5%~2.3% [38] CO 2, 8,,,, [39] 3,, 20 min,,,,, 4 h, [40],

4 8, : 3117,,, Brunton [41], Pusvaskiene [42] () ( )( ),, μg/l 甲酯化的优化,,, [43] -,, : min, 84.24, KOH ml, ml, 99.2% [44] GC-FID,,, 0.5%KOH- 647 μl 5 min 43, [45] / - - ( )2, -,,, [46] 5,, 2,,,, 色谱条件的优化,,, [39] HP-5 HP-INNOWax HP HP-88 40, 23 [47] (DB-FFAP) (CP-SIL88) 50% - (DB-225) DB-FFAP ; CP-SIL88, ; DB min 37 [48],, 1.5 μl, 75 ml/min, 220,, [49], 100 m 30 m 100 m,, ; 30 m 35 min, C18:1t,, 30 m HP GC-MS 检测技术的扩展,, GC-MS,, [50] HP-88,,, DHA EPA AA -, 90%~110% [51] HCl-BF 3 -,, - (GC-MS) HCl- BF 3 -,, [52] SPB ,,, ω-3( EPA DHA) ω-6 [53] -

5 3118 7,, -, 28, (26.57%) (17.6%) (7.34%) [54] - (GC-MS) 16,, PUFA EPA DHA ; PUFA EPA ARA Saini [55] 8,, α-(c18:3) 49%~59%, (C16:0) (16%~18%) (C18:2) (6%~13%),, 3 展望,,,, GC-MS,,,,,,,,,,,, 参考文献 [1],,. [J]., 2004, 25: Huang FH, Huang QD, Liu CS. Nutrition and balance of fatty acids [J]. Food Sci, 2004, 25: [2],,. [J]., 2008, 33(3): Chen YJ, Ju XR, Zhou GH. Classification and physiological function of saturated fatty acids [J]. Chin Oils Fat, 2008, 33(3): [3],. [J]., 2005, 4: Wang W, Zhang WM. Functional characteristic of monounsaturated fatty acids [J]. Food Nutr China, 2005, 4: [4],,,. [J]., 2012,7: Sun XY, Gao GT, Duan AL, et al. Research progress in polyunsaturated fatty acids[j]. Sci Technol Food Ind, 2012, 7: [5],,,. [J]., 2014, 31(2): Zhou WB, Lv CM, Zhang N, et al. Progress in the analytical methods for the determination of fatty acids [J]. Chin J Mod Appl Pharm, 2014, 31(2): [6] Makahleh A, Saad B, Siang Gh, et al. Determination of underivatized long chain fatty acids using RP-HPLC with capacitively coupled contactless conductivity detection [J]. Talanta, 2010, 81(1/2): [7],,,. HPLC-FLD [J]., 2011, 30(3): Zheng ZJ, Zhao XE, Chi BH, et al. HPLC-FLD analysis of free fatty acids in peanut oil [J]. Chin J Anal Lab, 2011, 30(3): [8] Xie Y, Li G, You J, et al. A novel labeling reagent of 2-(12-benzo [b] acridin-5-(12h)-yl)-acetohydrazide for determination of saturated and unsaturated fatty acids in traditional Chinese herbs by HPLC-APCI-MS [J]. Chromatography, 2012, 75(11/12): [9],,,. HPLC-MS [J]., 2014, 33(6): Hu N, Suo YR, Han LJ, et al. Determination and analysis of fatty acids in Lycium ruthenicum Murr fruits by HPLC-MS with pre-column derivatization [J]. Chin J Anal Lab, 2014, 33(6): [10] Casale M, Oliveri P, Casolino C, et al. Characterisation of PDO olive oil Chianti classico by non-selective(uv-visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques [J]. Anal Chim Acta, 2012(712): [11] Vongsvivut J, Heraud P, Zhang W, et al. Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements using Fourier transform infrared(ftir) spectroscopy [J]. Food Chem, 2012, 135(2): [12] Knothe G, Kenar JA. Determination of the fatty acid profile by 1H-NMR spectroscopy [J]. Eur J Lipid Sci Technol, 2004, 106(2): [13] SN/T EPA DHA - [S]. SN/T Determination of eicosapentaenoic acid (EPA) and docosahexaenoic acid(dha) in foods for export-gas chromatography [S]. [14] GB/T [S]. GB/T Determination of eicosapentaenoic acid and docosahexaenoic acid in foods [S]. [15] GB/T [S]. GB/T Determination of trans fatty acids in foods-gas chromatographic methods [S]. [16] SN/T [S]. SN/T Determination of trans fatty acids in foodstuff and oils-ftir method [S]. [17] GB [S]. GB National food safety standard Determination of trans fatty acids in foods for infants and young children milk products [S]. [18] GB

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Microsoft Word - 3098-3102 20150611007-蔡伟江-二校

Microsoft Word - 3098-3102 20150611007-蔡伟江-二校 6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug., 2015 蔡伟江 *, 邓鹏飞, 刘光兰, 李丹 (, 519040) 摘要 : 目的, 方法 (4.6 mm 250 mm, 5.0 μm) ; : : : =70: 16: 14(V:V:V),, : 1.0 ml/min; : 40 ; : 10