Microsoft Word 魏 静-二校 929

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1 6 3 Vol. 6 No Journal of Food Safety and Quality Mar., 2015 魏 静 1,3, 李幼梅 2, 谷满屯 2, 姬彦羽 2, 张伟敏 2* (1., ; 2., ; 3., ) 摘要 : 目的 方法, Stata 12, 结果 kg/d; 1,, 99.1%,, 结论,, 关键词 : ; ; Exposure assessment of the content of trans fatty acids of formula milk powder for infant and young children in Hainan Province WEI Jing 1,3, LI You-Mei 2, GU Man-Tun 2, JI Yan-Yu 2, ZHANG Wei-Min 2* (1. Food Inspection & Testing Center of Hainan Food and Drug Administration, Haikou , China; 2. College of Food Science, Hainan University, Haikou , China; 3. Hainan Provincial Products Quality Supervision and Inspection Institute, Haikou , China) ABSTRACT: Objective To assess the risk of infant and young children intake of trans-fatty acids (TFA) by consumption of formula milk powder. Methods The content of trans fatty acids of formula milk powder for infant and young children was determined by gas chromatographic method. Hazard quotient was employed to characterize the dietary risk of trans-fatty acids via eating formula milk powder for infant and young children with the application of Stata 12 software basing on Monte Carlo simulation. Results The results showed the average daily exposure dose of intake of TFA in Hainan province was kg/d. The average and each percentile of the hazard quotient of general population through consuming formula milk powder for infant and young children were all less than 1. Moreover, the sensitivity of the content of TFA of formula milk powder was strongest, accounting for 99.1%. The even time was negative, it was indicated that it had negative effects on daily exposure of 基金项目 : (314178) Fund: Supported by the Natural Science Fund of Hainan Province (314178) * 通讯作者 :,, zhwm1979@163.com *Corresponding author: ZHANG Wei-Min, Associate Professor, College of Food Science and Technology, Hainan University, No. 58, Renmin Avenue, Meilan District, Haikou , China. zhwm1979@163.com

2 930 6 TFA. Conclusion Therefore, to the general infant and young children, the dietary exposure of TFA only by formula milk powder was unlikely to bring about severe health risk. But the control and monitoring of TFA in formula milk powder should be strengthened to further reduce the dietary TFA risk level of the infant and young children. KEY WORDS: powdered infant formula; trans-fatty acids; exposure assessment 1 引言 25% 31% [1],, (trans fatty acids, TFAs), [2-4],, : ( ), FDA [5,6] ; 5%( ), 5% ( 1%), 3.8%, 5%, 3% 2%, [7] 2%, 0.01% [8] [9,10],, 2 材料与方法 2.1 样品采集, 试验材料与仪器 主要试剂 ( 10%); 60 g/l ; : ; (C18:0) -9- (C18:1-9t) -9- (C18:1-9c) -9, 12- (C18:2-9t, 12t) -9, 12- (C18:2-9c, 12c) (99%), Sigma, 仪器 Agilent 7890A ( )( ); BP221S ( ); ( ); RE52AA (); HH-4 ( ); HSC-12A ( ); TGL-10B (); ( ) 2.3 方法 GB [11] 2.4 风险评估方法 风险评估软件 Monte Carlo Stata 12, Stata 膳食数据 2010 (GB ), 3% (CODEX STAN ), 3%,,,, 3%(

3 3, : 931, 0.6 g 0.9 g) 反式脂肪酸的参考剂量, 12, 1 3,, ,, [12] WHO/FAO, (RfD)2.2 g/( ), 1%, 2 g [13-15],,,,,,, (RfD) WHO/FAO 2.2 g/( ) 风险评估的数学模型 (HQ, hazard quotient), Monte-Carlo, (RfD), HQ,, HQ<1, ; HQ>1,,, [16,17] Cf * PIR * ABS * EF * ED HQ AT * RfD *100 3 结果与讨论 3.1 色谱条件的选择 GB , HP-88,, -, 1 1 : HP-88 15,,, ; :,,, [18] 1 - Fig. 1 Gas chromatogram of cis-and trans-fatty acids

4 婴幼儿配方奶粉中反式脂肪酸的测定 GB ,, , 307, 0 g g/100 g, 3%, -, P 0.268, TFA 0, = , TFA 0 1, 1 -, p 0.516, TFA 0 表 1 婴幼儿配方奶粉中反式脂肪酸含量 (TFA) 含量分布 (%) Table 1 Distribution statistics for TFA concentrations in formula milk powder for infant and young children (%) 307 P 50 P 90 P 95 P LOD LOD LOD LOD LOD LOD LOD LOD (TFA) Fig. 2 Distribution statistics for TFA concentrations in formula milk powder for infant and young children 3.3 暴露评估模型参数的确定 婴幼儿配方奶粉中反式脂肪酸含量分布拟合, 307, TFA 0, Kolmogorov-Smirnov Stata 12, 3 Fig. 3 Distribution fitting of TFA concentrations in formula milk powder for infant and young children 反式脂肪酸日均暴露量的计算 Monte-Carlo 2

5 3, : 933 Table 2 表 2 基于 Monte-Carlo 方法的婴幼儿配方奶粉摄入反式脂肪酸风险商值的计算参数 The parameters of the calculation of hazard quotient through formula milk powder for infant and young children based on Monte-Carlo method (RfD), kg/d (PIR), kg/d (ABS) 1 (EF), d/ 365 (Cf)/% 0, , p =0.516, = (ED)/ =69.63, =73.33 (AT)/d =25415, =26765 (CDI), kg/d (HQ) Cf PIR ABS EF ED CDI AT 100 Cf HQ * PIR* ABS * EF * ED AT * RfD * 基于 Monte-Carlo 方法的婴幼儿配方奶粉中反式脂肪酸日均暴露量计算 2, Stata 12,, 10000, , 4 5, kg/d, 0 kg/d, kg/d, 1, 3.5 敏感度分析 Stata 12, 4 4,, 99.1%,,,,, 4 Fig. 4 The probabilistic distribution of chronic daily intake about the trans-fatty acids through formula milk powder for infant and young children 5 Fig. 5 The probabilistic distribution of hazard quotient about the trans-fatty acids through formula milk powder for infant and young children

6 934 6 表 3 婴幼儿配方奶粉中反式脂肪酸含量 (TFA) 日均暴露量与风险商 Table 3 Distribution statistics for TFA concentrations and hazard quotient in formula milk powder for infant and young children /kg/d P 50 P 90 P 95 P LOD LOD LOD LOD LOD LOD LOD LOD 表 4 反式脂肪酸日均暴露量影响因子的敏感度分析 Table 4 The exposure assessment and sensitivity analysis of exposure factors of the trans-fatty acids /% 结论 ED 0.50 AT 0.15, kg/d, 0 kg/d, kg/d,,,,,,,,,,,, 参考文献 [1]. [M]. :, Guo BH. Milk [M]. Beijing: Chemical Industry Press, [2] Kummerow, FA. The negative effects of hydrogenated trans fats and what to do about them [J]. Athsbl, 2009, 205(2): [3] Filip S, Fink R, Hribar J, et al. Trans fatty acids in food and their influence on human health [J]. Food Technol Biotechnol, 2010, 48(2): [4] Mozaffarian D. Trans fatty acids-effects on systemic inflammation and endothelial function [J]. Athsbl Supp, 2006, 7(2): [5],,. [J]., 2006, (11): Song ZH, Shan L, Wang XG. Developments in analytical methods for trans fatty acids [J]. Cereals Oils Proc, 2006, (11): [6],, 飏,. [J]., 2007, 23(2): Lai XY, Wu DY, Wu Y, et al. Hazards and determination of trans fatty acids [J]. Mod Food Sci Technol, 2007, 23(2): [7],,. [J]., 2009, 24(1): Xue YL, Zhao HY, Zhang R. Present status of trans fatty acids in edible vegetable oils in China [J]. J Chin Cereals Oils Assoc, 2009, 24(1): [8],. [J]., 2011, 27(4): He ZY, Wu C. Risk aeeseement on trans fatty acids in foods [J]. Food Mach, 2011, 27(4): [9] GB [S]. GB National food safety standard Infant formula [S].

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