Microsoft Word 王建梅-二校

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1 7 2 Vol. 7 No Journal of Food Safety and Quality Feb., 2016 * 王建梅, 刘河疆, 马玉娥, 安冉, 孙婷, 杨莲 (, ( ),, ) 摘要 : 目的 -, 方法, -, 结果 28, (26.57%) (17.6%) (7.34%) 38.83%, 61.17% 结论,,, 关键词 : ; ; ; - Determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry WANG Jiang-Mei, LIU He-Jiang, MA Yu-E, AN Ran, SUN Ting, YANG Lian * (Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Science, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture, Key Laboratory of Agroproducts Quality and Safety of Xinjiang, Urmqi , China) ABSTRACT: Objective To establish a method for determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry (GC-MS). Methods Fatty acids extracted by the mixture of hexane and aether were converted to methyl esters with KOH-CH 3 OH. Then the methyl esters were determined by the area normalizing method. Result There were 28 kinds of fatty acids in Apocynum honey, with the main components of linoleic acid (26.57%), palmitic acid (17.6%), and heptadecanoic acid (7.34%). The content of saturated fatty acid and polyunsaturated fatty acid were 38.83% and 61.17%, respectively. Conclusion Apocynum honey was rich in polyunsaturated fatty acid. This study provides scientific basis for exploitation and utilization of Apocynum honey. KEY WORDS: Apocynum; honey; fatty acids; gas chromatography-mass spectrometry 基金项目 : (KY ) ( ) Fund: Supported by Basic Scientific Research Nonprofit Funds of the Xinjiang Uygur Autonomous Region (KY ) and the Project of Technology Innovation Outstanding Talents in Xinjiang Uygur Autonomous Region ( ) * 通讯作者 :,, @qq.com *Corresponding author: YANG Lian, Experimentalist, Institute of Quality Standards & Testing Technology for Agroproducts, Xinjiang Academy of Agricultural Science, No. 403, Nanchang South Road, Urumqi , China @qq.com

2 引言 (Apocynum venetum),, 1952,,, [1-3], [4-6] ; [7],,, [8], - [9-11],,, - (gas chromatography-mass spectrometry, GC-MS),,, 2 材料与方法 2.1 原材料, 2.2 试剂,, ;,, ;,, Fisher Scientific 2.3 仪器 Clarus 500 ( Perkin Elmer ), (EI) ; PB303-E ( - ); N-1100 ( EYELA ) 2.4 方法 样品处理 2.50 g 10 ml, 2.5 ml - (2:1, V:V), 1 min; 2.5 ml 2.5 ml 2 mol/l -,, 1 min,, 25 min, 2.5 ml,,, 1 μl, GC-MS 仪器条件 (GC) : : HP-5MS (30 m 0.25 mm, 0.25 μm); : (99.999%); : 1.0 ml/min; : 1.0 μl, ; : 250 ; : 100, 20 /min 280, 16 min; (MS) : : (EI); : 70 ev; : 230 ; 220 ; : 3 min; : (SCAN), : 50~450 amu 脂肪酸组分定性定量分析 GC-MS NIST2011, GC-MS,, 3 结果与分析 3.1 样品中脂肪酸的成分分析,,, 仪器的重现性 3, (CV) 10%,, 3.3 方法的重复性 , (RSD),, 1

3 2, : Fig. 1 Full scan mass chromatogram of fatty acids methyl ester in Apocynum honey : 1 (C6:0); 2 (C12:0); 3 (C14:1); 4 (C14:0); 5 (C15:0); 6 (C16:1); 7 (C16:0); 8 (C17:1); 9 (C17:0); 10 γ- (C18:3n6); 11 (C18:2n6t); 12 (C18:2n6c); 13 (C18:1n9c); 14 (C18:0); 15 (C20:4n3); 16 (C20:5n3); 17 (C20:3); 18 (C20:2); 19 (C20:1); 20 (C20:0); 21 (C22:6n3); 22 (C22:5n3); 23 (C22:4); 24 13,16- (C22:2); 25 (C22:1n9); 26 (C22:0); 27 (C24:1); 28 (C24:0) Table 1 表 1 罗布麻蜂蜜中脂肪酸甲酯化学组分及含量 Composition and content of fatty acids methyl ester in Apocynum honey CAS min (%) RSD % 1 C6: ( ) C12: ( ) C14: ( ) C14: C15: ( )* C16: ( ) C16: * C17: C17: ( )* C18:3n ( ) * C18:2n6t ( ) * C18:2n6c ( ) * C18:1n9c ( ) C18: ( )* C20:4n (EPA-M) * C20:5n * C20: * C20: ( )* C20:

4 782 7 续表 1 CAS min (%) RSD % 20 ( ) C20: (DHA-M) * C22:6n (DPA) * C22:5n * C22: ,16- * C22: ( )* C22:1n ( ) C22: ( )* C24: ( ) C24: : * 4 讨论, 34.75%,, : = / 100% [8],,, ; 4,, ;,, 47.05%, ,, DHA DPA,,,, 5 结论 GC-MS,, 28, (26.57%) (17.6%) (7.34%), ; (SFA) 38.83%, 61.17%, (MUFA) 14.12%, (PUFA), 47.05%, [13-17] ; SFA/MUFA/PUFA 1:0.36:1.21; n %, n %, 9.14:1; 6.15% 参考文献 [1],. : [J]., 2005, 7(20): 8 Gao SL, Guan QH. Healthcare plants: Apocynum [J]. Chin Commun Doct, 2005, 7(20): 8 [2]. [M]. :, 1977.

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