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1 Chinese Journal of Applied Entomology 2016, 53(5): DOI: /j.issn 日本弓背蚁雄蚁生殖系统结构 * 及其化学组成研究 ** 李长有 *** 王云果贺虹摘要目的 Camponotus japonicus 方法 GC-MS 结果 /3 2 GC-MS % 21.87% 20.56% 12.71% 15.05% % % % 结论关键词 The male internal reproductive system, including chemical compounds, of the carpenter ant Camponotus japonicus Mayr LI Chang-You ** WANG Yun-Guo HE Hong *** (College of Forestry, Key Laboratory of Comprehensive Forestry of Shaanxi Province, Northwest A&F University, Yangling , China) Abstract [Objectives] To describe the internal reproductive system of male Camponotus japonicus, and analyze chemical compounds associated with this system, in order to provide baseline data for further study of male reproductive behavior. [Methods] The male internal reproductive system was observed under optical and scanning electron microscopy (SEM), and associated chemical compounds were analyzed using GC-MS. [Results] (1) The male reproductive system consists of one pair of accessory glands and one pair of vas deferentia and an ejaculatory duct; the testes degenerate and eventually disappear. The paired accessory glands are developed and milky white. The vas deferentia are simple and slender, and each connects with the lower third of each lobe of the accessory gland at the base. The surface of the accessory glands is relatively smooth and can be seen to be covered in muscle tissue under SEM. (2) Thirteen chemical compounds were identified, mainly comprised of aldehydes, olefins and acids, accounting for 29.31%, 21.87% and 20.56%, of the total, respectively. Some alcohols (12.71%) and esters (15.05%) were also detected. The most abundant components were cctadecatrienal (15.86%), 11-hexadecenal (13.45%), and 9,12,15-18 carbon triene acid-2,3-2 hydroxy propyl ester (11.78%). [Conclusion] The male reproductive system is comprised of three parts; the accessory glands, which are fully developed, the vas deferentia, which are slender, and the testes, which degenerate and disappear. Thirteen chemical compounds were found in the male reproductive system but their functions have yet to be investigated. Key words Camponotus japonicus, male ant, reproductive system, chemical compound * Supported projects No , No ** First author @qq.com *** Corresponding author hehong@nwsuaf.edu.cn Received Accepted

2 5 : 1119 Formicidae Hölldobler and Wilson 1990 Ball and Vinson 1984 Wheeler et al Wheeler and Krutzsch et al 1992 Heinze and Tsuji 1995 Keller et al Ayasse et al Mikheyev Jones et al Chenand Grodowitz et al Gotwald and Burdette 1981 Gillott 2003 Mikheyev 2003 Camponotus japonicus Mayr 竑 Blochmannia Sauer et al He et al Li et al 材料与方法 1.1 蚂蚁采集及腺体解剖 % Motic SMZ 扫描电镜样品的制备观察 2.5% 1.0 ml 1.5 ml 4 12 h 0.1 mol/l min 30% 50% 70% 80% 90% 100% JEOL JSM-6360 LV 1.3 化学成分分析 5 2 h GC-MS LTQ-XL min 20 /min min 10 /min min 1 L 70 ev NIST05

1120 应用昆虫学报 Chinese Journal of Applied Entomology 2 结果与分析 2.1 雄蚁生殖系统结构 2.

3 1120 应用昆虫学报 Chinese Journal of Applied Entomology 2 结果与分析 2.1 雄蚁生殖系统结构卷雄蚁生殖系统的化学组成雄蚁生殖系统的总离子流图如图 2 主要根据保留时间和质谱库进行初步辨认共鉴定出日本弓背蚁雄蚁的生殖系统比较简单主要包括 1 对附腺 1 对输精管和射精管 3 部分图 1 A 附腺最为发达乳白色不透明湿润饱满附腺两部分大小形状相同都为肾形长约 1.33 ± 0.21 mm 宽约 0.5 ± 0.2 mm 基部合并连接到射精管 1 对细长的输精管分别与附腺在其基部 1/3 的位置相连白色半透明每个输精管长约 1.5 ± 0.32 mm 直径约 0.14 ± 0.05 mm 输精管顶端的睾丸退化消失 13 种化学物质表 1 其中以醛类烃类和酸类为主分别占 29.31% 21.87%和 20.56% 还有少量的醇类和酯类分别占 12.71%和 15.05% 其中酸类 4 种分别是正十六酸 17-十八炔酸 9-十六烯酸和 7-十八烯酸烃类 3 种包括 7-十八碳烯 9-十九烯和 10-二十一烯醛类两种分别为 11-十六烯醛和十八烯醛还含有两种醇类 13-十七烯-1-醇环十五醇和两种酯类 9 在扫描电镜下生殖副腺和输精管表面平十八碳三烯酸-2 3-二羟基丙酯和 4-羟基- 坦没有明显的突起和其他结构输精管直径不十八碳酸甲酯其中含量最高的 3 种成分分别均匀图 1 B C 射精管细长图 1 B 为十八烯醛 15.86% 11-十六烯醛 13.45% 在更高的倍数下发现生殖副腺表面包裹发达的和十八碳三烯酸-2 3-二羟基丙酯网状肌肉组织图 1 D 11.78% Fig. 1 图 1 日本弓背蚁雄蚁生殖系统结构 Structure of male reproductive system of Camponotus japonicus A. 光学显微镜下雄蚁生殖系统结构 B C D. 扫描电镜下雄蚁生殖系统的形态特征 Ag 附腺 Ed 射精管 Mf 肌肉纤维 Vd 输精管 A. Structure of male reproductive system under the optical microscope; B, C, D. Morphology of male reproductive system under the SEM; Ag: Accessory gland; Ed: Ejaculatory duct; Mf: Muscle fiber; Vd: Vas deferens.

4 5 : 1121 图 2 日本弓背蚁雄蚁生殖系统化学成分 GC-MS 总离子流图 Fig. 2 GC-MS total ion current chromatograms of chemical component derived from male reproductive system of Camponotus japonicus 表 1 日本弓背蚁雄蚁生殖系统化学成分的 GC-MS 鉴定结果 Table 1 Identification of chemical components in male reproductive system of Camponotus japonicus by GC-MS Retention time min Compounds Formula % Relative content % Kinds C 18 H C 18 H 34 O C 17 H 32 O C 19 H C 16 H 30 O C 21 H C 15 H 30 O C 16 H 32 O C 18 H 32 O ,12,15- -2,3- C 21 H 36 O C 16 H 30 O C 19 H 38 O C 18 H 34 O , Si5~ Si10 Si7 3 讨论 Gotwald and Burdette 1981 Ball and Vinson 1984 Wheeler and Krutzsch 1992 Mikheyev Chen et al Wheeler Camponotus festinatus C. sayi C. mina Gotwald and Burdette 1981

5 1122 Chinese Journal of Applied Entomology 53 Mikheyev Jones 2010 Myrmicinae Monomorium Solenopsis 4 Mikheyev 2003 GC/MS Solenopsis invicta Bombus terrestris Baer et al Chen Grodowitz 2016 Wheeler Krutzsch 1992 SDS- PAGE 3 GC-MS Mikheyev 参考文献 (References) Ayasse M, Paxton RJ, Tengö J, Mating behavior and chemical communication in the order Hymenoptera. Annual Review of Entomology, 46: Baer B, Maile R, Schmid-Hempel P, Morgan ED, Jones GR, Chemistry of a mating plug in bumblebees. Journal of Chemical Ecology, 26(8): Ball DE, Vinson SB, Anatomy and histology of the male reproductive system of the fire ant, Solenopsis inuicta. Journal of Insect Morphology & Embryology, 13(4): Chen J, Grodowitz MJ, Tyramides in male alates of black imported fire ants, Solenopsis richetri (Hymenoptera: Formicidae). Insect Science, 17(3): Dai DC, Wang ZW, Li GH, Ge BW, Xu JS, Zheng HQ, The research of Camponotus japonicus and the roles to control the pine moth. Forest Pest and Disease, 5(1): 4 6. [,,,,,, , 5(1): 4 6.] Gao WT, Meng QF, Liu S, Bionomics of Camponotus japonicas. Forest Pest and Disease, 24(4): [,,, , 24(4): ] Gillott C, Male accessory gland secretions: modulators of female reproductive physiology and behavior. Annual Review of Entomology, 48: Gotwald WH, Burdette AW, Morphology of the male internal reproductive system in army ants: phylogenetic implications (Hymenoptera: Formicidae). Proceedings of the Entomological Society of Washington, 83(1): He H, Chen YY, Zhang YL, Wei C, Bacteria associated with gut lumen of Camponotus japonicus Mayr. Environ. Entomol., 40: He H, Wei C, Wheeler DE, The gut bacterial communities associated with lab-raised and field-collected ants of Camponotus fragilis (Formicidae). Current Microbiology, 69(3):

6 5 : 1123 Heinze J, Tsuji K, Ant reproductive strategies. Researches on Population Ecology, 37(2): Hölldobler B, Wilson EQ, The Ants. Cambridge: Harvard University Press Jones TH, Garraffo HM, Spande TF, Andriamaharavo NR, Gorman JS, Snyder AJ, Jeter, AW, Torres JA, Snelling RR, Daly JW, Caste-specific tyramides from Myrmicine ants. Journal of Natural Products, 73(3): Keller L, Sundström L, Chapuisat M, Male reproductive success: paternity contribution to queens and workers in Formica ants. Behavioral Ecology & Sociobiology, 41(1): Li XP, Nan XN, Wei C, He H, The gut bacteria associated with Camponotus japonicus Mayr with culture-dependent and DGGE methods. Current Microbiology, 65(5): Mikheyev AS, Evidence for mating plugs in the fire ant Solenopsis invicta. Insectes Sociaux, 50(4): Mikheyev AS, Male accessory gland size and the evolutionary transition from single to multiple mating in the fungus-gardening ants. Journal of Insect Science, 4(1): 37. Sauer C, Stackebrandt E, Gadau J, Hölldobler B, Gross R, Systematic relationships and cospeciation of bacterial endosymbionts and their carpenter ant host species: proposal of the new taxon Candidatus Blochmannia gen. nov. International Journal of Systematic & Evolutionary Microbiology, 15(3): Shang YC, The social life of ant. Bulletin of Biology, 41(4): 5 7. [, , 41(4): 5 7.] Wheeler DE, Krutzsch PH, Internal reproductive system in adult males of the genus Camponotus (Hymenoptera: Formicinae). Journal of Morphology, 211(3): Wheeler DE, Crichton EG, Krutzsch PH, Comparative ultrastructure of ant spermatozoa (Formicidae: Hymenoptera). Journal of Morphology, 206(3): Wu J, Wang CL, The Ant of China. Beijing: Chinese Forestry Publishing House. 1 10, [,, :. 1 10, ] Yin SH, Yang LZ, The morphology and life habits of Camponotus japonicus. Chinese Bulletin of Entomology, 23(5): [ 竑,, , 23(5): ] Zhan GJ, Xi GS, Observation on inner reproductive system of Polyrhachis vicina Rog. Journal of Anhui Agricultural Sciences, 37(12): [,, , 37(12): ] Zhang J, Nan XN, Wei C, Wang YG, He H, Bacterial composition of the infrabuccal pocket of Camponotus japonicus Mayr. Chinese Journal of Applied Entomology, 53(1): [,,,,, , 53(1): ] Zhu JY, Ye GY, Hu C, Research progress on morphology, secretion and function of Dufour gland in Hymenoptera. Acta Entomologica Sinica, 50(6): [,,, , 50(6): ] Zhu ZL, Nan XN, Wang YG, He H, The isolation of anaerobic bacteria from the gut of Camponotus japonicus. Chinese Journal of Applied Entomology, 52(4): [,,,, , 52(4): ]

Microsoft Word - 793-797 tb20150504赵宏宇s-高校教改纵横.doc

Microsoft Word - 793-797 tb20150504赵宏宇s-高校教改纵横.doc 微生物学通报 Microbiology China tongbao@im.ac.cn Apr. 20, 2016, 43(4): 793 797 http://journals.im.ac.cn/wswxtbcn DOI: 10.13344/j.microbiol.china.150504 高校教改纵横生物工程专业发酵课程群建设探索 * 赵宏宇