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1 5 5 Vol. 5 No Journal of Food Safety and Quality May., 2014 李晓东 1,2,3*, 蒋琛 2, 宋惠敏 (1.,, ; 2., ; 3., ) 1,3 摘要 :,,,, ACE, ACE,,,,,,,, 关键词 : ; ; Research progress on preparing functional polypeptide from whey protein hydrolysis LI Xiao-Dong 1,2,3*, JIANG Chen 2, SONG Hui-Min 1,3 (1. Key Laboratory of Dairy Science of Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin , China; 2. Heilongjiang University of Chinese Medicine, Harbin , China; 3. Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin , China) ABSTRACT: With the development of research on biological activity of small peptides in recent years, research on functions of whey protein hydrolysate peptides become hot. Whey is a liquid by-product obtained in the process of cheese and casein. Whey is rich in nutrients, and its main component is whey protein which has a high nutritional value. Recent studies have found that whey protein contains a variety of bioactive peptides, including antihypertensive peptides, antimicrobial peptides, antiviral peptide, antioxidant activity peptides and other bioactive peptides, and has been a hot research. At present, there are many studies on the antihypertensive peptides at home and abroad, and there are fewer studies on hypocholesterolemic peptides. Most of studies about hypocholesterolemic peptides are about soy protein. However, whey protein hypocholesterolemic peptides have more research value, in fact. In this experiment, whey protein was as raw material, hypocholesterolemic peptides were separated and prepared from its hydrolysates. In this subject, by-product of cheese was full used, it not only reduced waste of resources and environmental pollution, but also provided a theoretical basis 基金项目 : (2011BAD09B01) Fund: Supported by the National "Twelfth Five-Year" Plan for Science and Technology Support Program (2011BAD09B01) * 通讯作者 :,,, hrblxd@163.com *Corresponding author: LI Xiao-Dong, Professor, Key Laboratory of Dairy Science of Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin , China. hrblxd@163.com

2 for the commercial development of hypocholesterolemic functional products. This paper reviews the whey protein source of functional polypeptides preparation, separation and purification technology and research status of active peptides. KEY WORDS: whey protein; bioactive peptides; bioactivity 1 引言,,,,,, [1], [2],, [3],, 1 9, [4],,, ( 1), [5],,, [6], [7],, Fig. 1 1 Physiological framework of bioactivity of whey peptides.

3 5, : 乳清蛋白生物活性肽的研究进展 : ACE [8-15], 1 表 1 乳清蛋白水解产生的生物活性肽的功能及其可能机制 Table 1 The function and possible mechanism of Bioactive peptides from whey protein hydrolysis / f( ) ACE (ET-1) α-lactorphin f(50 53) ACE ( ) β-lactorphin f( ACE NT2 β-lactotensin f( ) ACE NT2 α-lactorphin; β-lactorphin; serophin Albutensin C3a C5a NT2 D1 C3a 2.1 乳清蛋白肽抑菌抗病毒抗肿瘤活性的研究现状, 2007, Miralles [16] - ; 2004, Leonie [17],, 2.2 乳清蛋白肽免疫调节作用的研究现状,,, 2008, Gauthier [18], γ- 2.3 乳清蛋白抗氧化肽的研究进展, 2007, Cheison [19] Protease N Amano, DPPH ; 2009, Kong [20], 100~2800 Da 2.4 乳清蛋白 ACE 抑制肽的研究进展 ACE, 1982 Maruyama ACE Mullally [21] β- ACE β- ACE, ACE 83.4% Pihlanto-Leppala [22], ACE, ACE, IC50 77~1062 μm Lee [23] K, 6 ACE, ; Ferreira [24] ACE,, Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR) ACE Elizabete [25] ACE, 65 95,, 65 ACE Guo [26] L. Helveticus, E/S 0.60, ph 9.18, 38.9 ACE 82.2%, 18.8% Lee [27] β- α- ACE β- α-,, α- ACE, C- α IC 50 1~5 μm β- ACE f58-76 f59-76 Tânia [28], ACE,, ;,

4 ; 2012 ACE [29] ACE,,, ACE [30], 2.5 乳清蛋白降胆固醇肽的研究进展,,,, [31] LPYPR IAVPGEVA A (HMGR) [32], 2001 Nagaoka Nagaoka [33],,, 1999 Nagaoka [34],,,, 2001 Nagaoka [35] β- (β-lactoglobulin), 5 (Ile-Ile-Ala-Glu-Lys), f 71-75, Caco-2 5,,,, 2006 [36], 2000~12000 Da HMG-CoA A (HMGR) Kensei Lactostatin CYP7A1 mrna [37],,, 2.6 乳清蛋白降血糖肽的研究进展 2007, Blouet [38] -La Wistar, 2 h 2011 [39],, 900~1900 Da, KKAy 0.5 h 2.7 乳清蛋白肽的其他功能性研究现状 2009, Xu [40] 1.4~3.4 kda,,, 2010, [41],,,, 2011, Malcata [42],, 3 乳清蛋白降胆固醇活性肽的制备及初步纯化的研究 3.1 乳清蛋白降胆固醇活性肽分离纯化研究现状, [43-46] ( 2):,, [46-50], 2

5 5, : 1409 Fig. 2 2 The preparation of functional peptide from Whey protein Table 2 表 2 乳清蛋白降胆固醇活性肽的分离纯化方法 The methods of separation and purification of whey protein hypocholesterolemic peptides,,,,, 1 kda~15 kda,,,, 1 kda,,,, (SDS-PAGE) 3.2 酶法制备乳清蛋白降胆固醇活性肽及初步纯化的研究 WPC80,, : (1) :,, : 8.5 h 55 ph % 5.8%, 17.26% (2) : 10 kda,, : 40, 0.15 MPa, 6 mg/ml, 28.63%,,,, 93.14%, 85.13%, 28.63% 47.53% Sephadex G-50 Sephadex G-25, Sephadex G-50 F %, Sephadex G % FB

6 (3): Tricine-SDS-PAGE, Y = X (R 2 = ), 1.9~3.3 kda (4) :, 90.18%, 5.37%, 0.85% (5) :,, 81.12% 76.85%, 3.3 下一步研究计划,,,, : 12α α-(CYP7A1), - (LXR) (FXR),, 4 结语,,,,, 岀, : (1) Caco-2 ; (2) ; (3),, 参考文献 [1]. [D]. :, Xu Q. Effects of medium-chain fatty acids on cholesterol metabolism and their mechanisms in mice [D]. Beijing: Academy of Military Medical Sciences, [2] Salter AM. Dietary fatty acids and cardiovascular disease. Animal: an internationaljournal of animal [J]. Biosci, 2013, 7(Suppl 1): [3]. [M]. :, Gu RX. Physiological features of milk and dairy producs [M].Beijing: China Light Industry Press, [4] Ryan KN, Vardhanabhuti B, et al. Stability and mechanism of whey protein soluble aggregates thermally treated with salts [J]. Food Hyd, 2012, 27, [5] Yuma T, Masaya S, et al. L-Cysteine-induced up-regulation of the low-density lipoprotein receptor is mediated via a transforming growth factor-alpha signaling pathway [J]. Biochem Biophy Res Communications. Available online, [6] JacobsonTA, Glickstein SB, et al. Effects of eicosapentaenoic acid and docosahexaenoic acid on low-density lipoprotein cholesterol and other lipids:a review [J]. J Clinical Lipidol, 2012, 6(1): [7] [EB/OL] / html, The interpretation of food industry 12th Five-Year plan [EB/OL] / html, [8] Maes W, Camp JV, et al. Influence of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR) on the release of endothelin-1 by endothelial cells [J]. Regul Pept, 2004, 118: [9] Sipola M, Finckenberg P, et al. Effect of long-term intake of milk products on blood pressure in hypertensive rats [J]. J Dairy Res, 2002, 69: [10] Yamauchi R, Usui H, et al. Characterization of β-lactotensin a bioactive peptide derived from bovine β-lactoglobulin as a neurotensin agonist [J]. Biosci Biotechnol Biochem, 2003, 67(1): [11] Nurminen ML, Sipola M, Kaarto H, et al. α-lactorphin lowers

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Microsoft Word 马姜-出3355

Microsoft Word 马姜-出3355 6 9 Vol. 6 No. 9 2015 9 Journal of Food Safety and Quality Sep., 2015 马姜 1, 高原 1*, 王红静 2 (1.,, 100089; 2. ( ), 100080) 摘要 : 目的, 方法 HORIBA LA-950 结果,, 结论, 关键词 : ; ; ; Application of particle