2011 12 19 6 ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA December 2011 Vol. 19 No. 6 檵 檵檵檵檵檵檵檵檵檵 1 2 2 2 3 4 1 1 2 5 1. 400054 2. 400010 3. 400080 4. 721000 5. 400010 SD 2 mm 3 mm 4 55. 5% 13. 3% 36. 2% 66. 7% 100% QRS - T M R T ST 4 3 mm 2 mm ST SD Q95-33 R542. 22 A 1005-4847 2011 06-0525-05 Doi 10. 3969 / j. issn. 1005-4847. 2011. 06. 015 Method selection for the establishment of myocardial infarction model and the electrocardiographic presentation in the rats WANG Yong 1 GAO Da-zhong 2 YIN Yue-hui 2 SHE Qiang 2 GAO Cui-ju 3 ZhAO Jiang-long 4 TAN Yong 1 TANG Yong-gang 1 CHEN Yun-qing 2* LI Pan 5 1. Department of Cardiovascular DiseasesThe Seventh People s Hospital of ChongqingChongqing 400054China 2. the Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010 3. General Hospital of Chongqing Iron and Steel CompanyChongqing 400080 4. People s Hospital of Baoji CityBaoji 721000 5. Ultrasonic Imaging Institute of Chongqing Medical UniversityChongqing 400010 Abstract Objective To explore the site of left coronary artery ligation on establishing myocardial infarction model and the electrocardiographic features in those rat models. Methods After the Sprague-Dawley SD rats were anaesthetized and put on chine-assisted respirationan occluding suture was placed at one of two distinct sites along the left coronary artery. The suture was placed about 3 mm distal to the aortic root high and about 2 mm distal to the horizontal line of left atrial appendage tip low respectivelyand underwent the same procedure as the low suture group except left coronary artery ligation sham monitored by electrocardiography ECG before and after operationrespectively. At 4 weeks after operation they were sacrificed for histopathological analysis and measurement of the infarct area. Results The infarct area was about 55. 5% and 36. 2% and the total survival rate was 13. 3% and 66. 7% in the high and low suture groupsre- 2006 - B - 25 1973 - E-mail foot1972@ sohu. com E-mail chenyunqing. 88@ 163. com
526 2011 12 19 6 Acta Lab Anim Sci Sin December 2011 Vol. 19. No. 6 spectively. There was no myocardial infarction and the survival rate was 100% in the sham operation group. ECG QRS-T wave displayed an intersection M shape in the sham group and before left coronary artery ligation. The R wave and T wave fused into one large tent-like single wave after the left coronary artery ligationand without visible ST segment. Histopathological changes of myocardial infarction were seen at 4 weeks after operation. Conclusions It is a novel method to establish myocardial infarct model that the suture is placed about 2 mm distal to the horizontal line of left atrial appendage tipand all ECG showed no visible ST segment in the rats. Key words Rat Modelmyocardial infarction Electrocardiogram 1. 2. 2 70 / / 1 3 3 ml /100 g 1. 2. 3 10% 0. 3 ml /100 g 2 ~ 3 mm 1. 2. 4 1 ~ 5 50 mm / s 2 mv 10 min 20 min 6 ~ 8 1 1. 2. 5 SD 3 ~ 4 1 1. 1 1. 1. 1 SPF SD 45 2 ~ 3 259 ± 21 g SCXK 2007-0001 SYXK 2007-0001 3R 1. 1. 2 7-0 0. 5 ~ 1. 0 mm ALC-V 8 3 mm ECG- 6511 Canon-IXUS 850IS 2 mm RM2235 1. 2 1. 5 cm 1. 2. 6 1 9 3 4 3 4 1 1. 2. 1 45 SD 1. 2. 7 15 15 0 15 5 ml
2011 12 19 6 Acta Lab Anim Sci Sin December 2011 Vol. 19. No. 6 527 1. 4 Junya Takagawa 10 5 min 4 μmhe % = / /2 + 7-0 / /2 0 100% IPP 6. 0 1. 5 1. 2. 8 SPSS11. 5 30 P < 0. 05 7 d 2 1. 3 2. 1 4 492 ± 21 / P PR R 4% T R T M HE R T ST 1 A B P PR R T M ST C D P PR R T ST A B C D 20 min 1 Ⅰ Ⅱ Ⅲ avr avl avf Note A B Apparent P wave and PR intervalr and T wave intersection M without visible ST segment C D Apparent P wave and PR interval manifestr and T wave composition high tent wavewithout visible ST segment A the preoperative ECGB C D represent the ECG in sham operation low suture and high suture groups at 20 min after operationrespectively. Fig. 1 The ECG changes in different groups before and after the left coronary artery ligation ⅠⅡⅢavRavLavF
528 2011 12 19 6 Acta Lab Anim Sci Sin December 2011 Vol. 19. No. 6 20 min 521 ± 36 / 55. 5% P PR I II III AVF II III AVL AVF I II AVL AVF I II III AVL AVF II III AVF R T 7 24 h 11 2 13. 3% P PR R T R T M R T ST 1 P < 0. 05 1 100% 2. 2 1 Tab. 1 n = 15 Comparison of the mortalitysurvival rate and infarct area in the rats Group 1 h Within 1 h 24 h Within 2 h 36. 2% P < 0. 05 1 h 1 h 1 24 h 4 1 66. 7% 24 h After 24 h / % Total survival rate / % Infarct area High ligation group 7 11 2 13. 3 55. 5 Low ligation group 1 4 1 66. 7 36. 2 Sham operation group 0 0 0 100 0 P < 0. 05 Note Compared with the high suture groupp < 0. 05 a significant difference. 2. 3 11 12 HE 2 4 HE 3 4 13 3 2 ~ 3 mm 80% ~ 90% 2 ~ 5 73. 3% 4 66. 7% 1 5 3 mm 24 h 26. 7% 4 13. 3% 11 2 mm 24 h
2011 12 19 6 Acta Lab Anim Sci Sin December 2011 Vol. 19. No. 6 529 ST 492 ~ 521 / R T 20 ~ 30 min 2 3 4 Degabriele 11 2002 2 200917 6 419-423. 3 J 55. 5% 36. 2% 332. 6 Marc APfefferJanice M et al. ST ventricular function in ratsj. Circ Res 1 ~ 5 7 Brenner WAicher AEckey Tet al. P PR R T R T R T M ST R T J ST 2 ST 2 2005 26 15 1544 - l550. ST R T 2007102 2104-2111. Degabriele 11 3 mm 2 mm 1. J. 27 2 153-155.. J... 2004 35 1 13-15. 4. J. 2005 26 4 67-69. 5. J. 2008 30 4 329 - Myocardial infarct size and 197944 503-512. 111 In-labeled CD34 hematopoietic progenitor cells in a rat myocardial infarction model J. J Nucl Med 2004 45 512-518. 8 Gao EHLei YHShang XYet al. A novel and efficient model of coronary artery ligation and myocardial in farction in the mouse. Circ Res. 2010 107 1445-1453. 9 Mellin V Isabelle M Oudot A et al. Transient reduction in myocardial free oxygen radical levels is involved in the improved cardiac function and structure after long-term allopurinol treatment initiated in established chronic heart failure J. Eur Heart J 10Takagawa JZhang YWong MLet al. Myocardial infarct size measurement in the mouse chronic infarction model comparison of area- and length-based approaches J. J Appl Physiol 11Degabriele NMGriesenbach UDato Ket al. Critical appraisal of the mouse model of myocardial infarction J. Exp Physiol 200489 89 497-505. 12Lutgens EDaemen MJAPde Muinck ED et al. Chronic myocardial infarction in the mouse cardiac structural and functional changes J. Cardiovasc Res199841 1999 586-593. 13. J. 2008 42 2 147-149. 2011-05-03
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