2011 43 5 21 3 1 1* 1 2 3 1 1. 2. 3. 78. 68±2. 95 g 28 300 3 5 3% 3 1 ALT TP ALB AST 0. 05 P>0. 05 ALT TP ALB P>0. 05 AST 0. 05 GLOB P>0. 05 2 INS 0. 05 TSH T 3 0. 05 P>0. 05 GLU GH T4 IGF-I P>0. 05 S816. 48 A 0529-5130 2011 05-0021-05 Effects of three different protein-feed on the serum biochemical indexs and serum hormone in the green goose TANG Hai-rong 1 LIU Chang-zhong 1* ZHANG Hai-tang 1 CUI Jian-xun 2 Li Guo-ming 3 WANG Zi-liang 1 1. School of Animal Science Henan Institute of Science and Technology Xinxiang China 2. Xinxiang Tiandi goose co. Ltd. Xinxiang China 3. Daxing Feed Equipment co. Ltd. Yuanyang County Xinxiang China Abstract The objective of this study was to evaluate the effects of 3% of 3 kinds of protein-feed blood meal expanded blood meal and fish meal on the serum biochemical indexs and serum hormone in the green goose. A total of 300 huoyan geese 78. 68±2. 95 g BW 28 d of age were randomly divided into three treatments with five replicates. The results were as follows 1 the ALT TP ALB and AST of the fish meal group were significantly higher than that of the blood meal group 0. 05 but there was no significant difference between the fish meal group and the expanded blood meal group P>0. 05. The difference of ALT TP and ALB between the expanded blood meal group and the blood meal group were not significant P>0. 05. The AST of the expanded blood meal group was significantly higher than that of the blood meal group 0. 05. The difference of GLOB among the three groups were not significant P>0. 05. 2 The INS of the fish meal group was significantly higher than that of the blood meal group and the expanded blood meal group 0. 05. The TSH and T3 of the fish meal group were significantly higher than that of the blood meal group 0. 05 but there was no significant difference between the fish meal group and the expanded blood meal group P>0. 05. The difference of GLU GH T4 and IGF-1 among the three groups were not obvious P>0. 05. The results demonstrated that the expanded blood meal could partially replace the fish meal in the diet of green goose and the effect of the expanded blood meal on green goose is obvious superior to the blood meal. Key words blood meal fish meal expanded blood meal green goose 2010-09-21 2006BAK02A21 2010A230002 1977- E-mail thr9166_cn@ 126. com * 1971- E-mail liuchangzhong68@ 163. com 1 2 3 4 5 3
22 Animal Husbandry & Veterinary Medicine 2011 Vol. 43 No. 5 1 3% 3% 3% /% 65. 77 66. 61 66. 75 1 1. 1 1. 1. 1 81. 30% 6. 67% 1. 71% 1. 11% 0. 14% 0. 16% 1. 1. 2 + 83. 00% 6. 80% + 1. 72% 1. 11% 0. 15% 0. 17% 1. 1. 3 65. 40% 5. 34% + 2. 34% 0. 80% 1. 2 2. 50% 3. 66% 3. 0% 3 5 1. 3 1. 4 300 28 78. 68±2. 95 g 3 5 20 28 d 1. 5. 2 1. 5 28 1. 5. 1 2 10 28 3 ml 3 000 r /min 10 min 2 10-20 3 ml 4 1 500 r /min /% 15. 00 15. 00 15. 00 /% 10. 52 9. 14 8. 99 /% \ 3. 00 \ /% \ \ 3. 00 /% 3. 00 \ \ /% 0. 10 0. 18 0. 18 /% 0. 24 0. 67 0. 67 /% 5. 00 5. 00 5. 00 /% 0. 33 0. 33 0. 33 /% 0. 04 0. 07 0. 08 100. 00 100. 00 100. 00 /MJ kg -1 11. 50 11. 50 11. 50 /% 15. 01 15. 00 15. 00 /% 0. 80 0. 80 0. 80 /% 0. 40 0. 40 0. 40 /% 0. 95 0. 95 0. 95 + /% 0. 67 0. 67 0. 67 /% 0. 16 0. 16 0. 16 * A 30 000 U D 3 5 000 U E 20 U K 3 8 mg B 1 5 mg B 2 10 mg 60 mg B 6 5 mg 10 mg 3 mg 0. 1 mg 1 000 mg 1 mg B 12 20 μg 5 mg 100 mg 80 mg 100 mg 0. 1 mg 0. 15 mg 0. 4 mg Growth hormone GH -I Insulin-like growth factor- NRC I IGF-I thyroidstimulating 1 hormone TSH 3 5 3'-triiodothyronine T3 thyroxine T4 insulin INS glucagon Glu RIA 20 min -20 ci8200 ALT AST
2011 43 5 23 ALB GLOB TP 3 1. 6 SAS ANOVA Duncan 6 ALT AST ± 2 2. 1 3 2 3 AST ALT /U L -1 21. 6±4. 06 b 25. 8±3. 34 ab 27. 1±4. 06 a AST /U L -1 39. 2±5. 72 b 47. 2±4. 65 a 47. 8±4. 43 a ALB /g L -1 10. 42±1. 37 b 12. 34±1. 29 ab 13. 68±1. 54 a GLOB /g L -1 19. 78±2. 11 a 21. 82±1. 90 a 21. 92±3. 61 a TP /g L -1 30. 2±3. 42 b 34. 16±3. 06 ab 35. 6±4. 38 a P > 0. 05 0. 01 0. 05 TP ALB 2 2 TP ALB ALT ALB TP 0. 05 P>0. 05 AST 0. 05 GLOB P>0. 05 TP 2. 2 3 ALB 3 TSH T3 0. 05 12 P>0. 05 2 3 4 INS 0. 05 P > GLOB 0. 05 GLU GH T4 IGF-I P>0. 05 13 14 3 3 3. 2 3 Glu /pg ml -1 207. 96±9. 64 a 218. 29±18. 10 a 232. 11±21. 63 a INS /μu ml -1 5. 16±0. 80 b 5. 23±0. 86 b 5. 89±0. 34 a TSH /ng ml -1 0. 66±0. 12 b 0. 75±0. 27 ab 0. 82±0. 09 a GH /ng ml -1 0. 58±0. 07 a 0. 64±0. 07 a 0. 64±0. 09 a T 3 /ng ml -1 1. 09±0. 07 b 1. 30±0. 10 ab 1. 40±0. 15 a T 4 /ng ml -1 10. 08±0. 83 a 10. 61±1. 28 a 10. 64±0. 93 a IGF-I /ng ml -1 48. 52±7. 33 a 50. 94±3. 10 a 50. 35±4. 45 a 3. 1 3 ALT AST 7-8 ALT ALT ALT AST 9 AST TP ALB GLOB 10-11 INS B Glu A INS B
24 Animal Husbandry & Veterinary Medicine 2011 Vol. 43 No. 5 GH 23 J -1 IGF- 34-36. I 27 IGF-I 15 IGF-1 IGFBP-1 16 B 24 28 IGFBP- 1 17 GH IGF-I B- INS GH IGF-I INS α GH IGF-I Glu 18 Glu 29 GH INS IGF-I 4 Glu B INS D A Glu 19 Si- GLOB GLU GH T4 IGF-I mon 20 21 21-22 T3 ALB AST INS TSH T 3 T4 23 ALT TP ALB AST TSH T 3 T3 0. 05 P >0. 05 INS P < 0. 05 ALT TP ALB P>0. 05 AST 24 0. 05 - - TSH T3 - - GH 5D-Ⅲ 5D-Ⅰ 1. J. 2010 T3 25 5 13-14. 2. J. GH 2007 2 32-34. GH 3. J. 2009 37 21 9995-9996 10016. 4. 23 J. T3 6 Song K Shan A S Li J P. Effect of different combinations of enzyme preparation supplemented to wheat based diets on growth and serum biochemical values of broiler chickens J. Acta Zoonutri- mentasinica 2004 25-29. 26 7 T3 J. 2001 34-37. ALT TP J. 2003 9 7-8. 5. 1995 19 2 159-164.. 8.. 1999 1
2011 43 5 25 CO1 1 1* 1* 2 1 3 1 1. 010018 2. 010020 3. 015500 Parabronema skrjabini DNA 1 CO1 pmd19-t PCR DNAStar 5. 0 MEGA 4. 0 GenBank CO1 DNA CO1 689 bp CO1 Habronema microstoma Habronema muscae 86. 7% 0. 143 Litomosoides galizai 80. 1% 0. 229 47% CO1 CO1 S852. 73+1 A 0529-5130 2011 05-0025-06 2010-12-23 1985- * 30660124 Tel 0471-4303726 E-mail lianruyang122@ 163. com 200607010401 E-mail xiaoyeyang122@ sohu. com 櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔櫔 9. Poultry Science 1995 14-17. J. 2009 41 4 21. 64-67. J. 2004 16 4 10. 195-197. D. 2007. 22. T3 T4 11. J. 2008 J. 2010 42 7 3 106-109. 21-25. 23. 5 IGF-Ⅰ 12. J. J. 2003 21 8-9. 2006 38 12 4-7. 13. 24. D. 2005. J. 2007 35 33 14. 10718-10722. J. 25 Vasilatos-Younken R Wang X H Zhou Y et al. New insights into 2001b 4 18-20. the mechanism and actions of growth hormone GH in poultry 15. J. Domest Anim Endocrinol 1999 17 2-3 181-190. J. 5-8. 26. 16. J. 2009 28 2 7-11. -2 D. 2009. 17. J. - 2004 24 3 24-26. 18. M. 28. M. 1993 82-99. 2001 1-19. 20 Simon J. Insulin-glucagon and growth in broilers J. European 27. J. 2007 2 72-75. 19. - M. 29 Ramos S goya L Alvarez C et al. Effect of thyroxine adminostration on the IGF /IGF binding protein system in neonataland adult 1991 98-129. thyroidectomized rats J. Endocrinol 2001 169 1 111-122.