1410 HEREDITAS (Beijing) ancestry location of oversea Chinese, the surname origin and evolution, origin and migration of modern humans and man

HEREDITAS (Beijing) 2012 11, 34(11): 1409 1416 ISSN 0253-9772 www.chinagene.cn 研究报告 DOI: 10.3724/SP.J.1005.2012.01409 人类 Y 染色体 36 个新 STR 位点的筛选与鉴定 1, 彭冬铂 2, 姜正文 2 1,, 孙斯平 2, 李才华 2, 卢大儒 1 1.,,, 200433; 2., 201203 通过对 NCBI 数据库中的 Y-STR 位点和生物信息学预测的新 Y-STR 位点的分析, 选取了 133 个位点在 48 个全球分布的样本中进行检测验证, 获得 41 个可靠的高频位点, 其中 36 个为首次发现验证的新 Y-STR 位点 利用这 41 个 Y-STR 位点, 在 200 个上海随机男性样本中共发现 200 种单倍型, 实现了人群内个体间 100% 区分, 并通过对浙江江山一姜氏聚集地的 9 个 5 代以内无血缘关系的姜氏个体和 7 个上海随机人群的姜氏个体的单倍型的分析, 发现 6 个江山姜氏个体具较近的亲缘关系 ( 彼此差异位点在 2~4 个之间 ) 这 41 个 Y-STR 位点具备较高的信息度, 能有效区分群体内不同家族来源的个体, 这将有助于群体内个体间近代亲缘关系研究, 并有望在法医学法医个体识别 亲权鉴定, 人类起源 迁徙等研究中发挥重大作用 Y-STR; 多态性 ; 单倍型 ; 区分度 ; 近代亲缘关系 Screening and identification of 36 new STR loci in human Y chromosome PENG Dong-Bo 1,2, JIANG Zheng-Wen 2, SUN Si-Ping 1,2, LI Cai-Hua 2, LU Da-Ru 1 1. State Key Laboratory of Genetic Engineering, MOE Key Laboratory of Contemporary Anthropology, School of Life Science, Fudan University, Shanghai 200433, China; 2. Center for Genetic & Genomic Analysi, Genesky Biotechnologies Inc., Shanghai 201203, China Abstract: 133 candidate Y-STR loci were selected from NCBI STS database or by bioinformatics analysis in human Y-chromosome sequence, and were screened among 48 DNA samples around the world. Forty-one Y-STRs with high allelic frequency were validated, 36 of which were first reported. Two hundred haplotypes of the 41 STRs were identified among 200 randomly sampled male individuals in Shanghai, indicating 100% inter-individual discrimination. By network analysis of haplotypes of the 41 STRs among nine Jiang-surname male individuals with no consanguinity within 5 generations from a Jiang-surname individual gathering at Jiangshan, Zhejiang Province, and 7 Jiang-surname male individuals from the random shanghai population, 6 Jiang-surname individuals from Jiangshan were close with only 2-4 STR locus difference. These 41 Y-STR loci provide enough information by which individuals from each other with different early modern family origin can be effectively distinguished. This will promote studies on identification of non-lineal relationship in forensics, 收稿日期 : 2012 05 14; 修回日期 : 2012 07 13 作者简介 :,, Tel: 021-50802060- 22; E-mail: pengdongbo@163.com 通讯作者 :,,,, E-mail: drlu@fudan.edu.cn 致 谢 : 网络出版时间 : 2012-8-15 10:51:15 URL: http://www.cnki.net/kcms/detail/11.1913.r.20120815.1051.001.html

1410 HEREDITAS (Beijing) 2012 34 ancestry location of oversea Chinese, the surname origin and evolution, origin and migration of modern humans and many other studies of Contemporary Anthropology Keywords: Y-STR; polymorphism; haplotype; discrimination; early modern genetic relationship 1921, Painter [1] Y, Y 59 373 566 bp, 95% (Nonrecombining portion of the Y chromosome, NRY) [2] Y,,, (Short tandem repeat, STR), DNA, 2~6,, STR 10-3 / [3, 4], SNP (SNP 10-9 / [5] ).,, [6~14] SNP,, STR, Y-STR,, Y-STR, DYS19 DYS388 DYS389I DYS389Ⅱ DYS390 DYS391 DYS392 DYS393 DYS425 DYS426 DYS434 DYS435 DYS436 DYS437 DYS438 DYS439 [15], Y-STR, NCBI DYS 3, Y-STR, DYS, 48, 200,,, 9 5 7, 1 1.1 48 3 4 6 ( 2 2 2 ) 6 4 25 200,, 9, 5 1.2 1.2.1 Y-STR 位点预测 (1) Y-STR, 201 DYS STR ; (2) 10 Y-STR ; (3) Y-STR NCBI 3 (Refercese HuRef Celera), Y-STR ; (4), Y-STR, 133 Y-STR, 30 DYS, 103, 1 1.2.2 Y-STR 位点检测 PCR Y-STR, 5 4, 4 (FAM VIC NED PET) Primer3 (http://frodo.wi.mit.edu/

11 : Y 36 STR 1411 primer3), PolyN SNP, 2 Y-STR (1) 20 μl PCR, 1 Hotstar Taq, 0.1 μmol/l PCR, 2.4 mmol/l MgCl 2, 0.3 mmol/l dntp, 1 U Hotstar Taq (QIAGEN), 5~10 ng DNA, ddh 2 O PCR 95 10 min; 94 20 s; 65 40 s, 72 2 min, 10, 1 ; 94 20 s, 63 40 s, 72 2 min, 20 ; 72 2 h (2) 20 µl 1 HotStar Taq, 2.0 mmol/l MgCl 2, 0.2 mmol/l dntp, 0.1 µmol/l 4, 1 U Hotstar Taq (QIAGEN), 1 µl PCR, ddh 2 O 95 10 min; 94 20 s, 65 40 s, 72 3 min, 30 (3) 10, LIZ500 (0.2 µl LIZ500+8.8 µl Hi-Di+1 µl PCR )95 5 min, ABI3130 l, GeneMapper4.0(Applied Biosystems ) 1.2.3 Y-STR 数据分析 1.2.3.1 Y-STR 等位基因的命名 Y-STR DNA (ISFG DNA Commission) [16, 17] Y-STR PCR 1, 1.2.3.2 男性个体区分度 Y-STR PDM (Power of Discrimination for Males), Y-STR PDM, PDM 1 n =, X STR, PDM=1- [18] 1.2.3.3 Network 软件分析样本之间遗传亲缘关系 Network, i X 2 i, ( ),, [19] Network MJ(Median-Joining network) RM(Reduced Median network) MJ Y-STR [20],,,,,, 2 2.1 Y-STR 48,, 133 Y-STR 41 Y-STR ( 5 DYS, 36 ), 3, 1, 3 panel 1 2.2 Y-STR PDM(Power of discrimination for males) 200, 41 Y-STR, 200 200 ( 3), 100%, 41 Y-STR 36 Y-STR, 41 Y-STR 0.8514(S85), 0.5 28 ( 2) 3.3 Network 200, Network ( 3, ) 41 Y-STR,,,

1412 HEREDITAS (Beijing) 2012 34 表 1 41 个 Y-STR 位点检测信息 STR# Repeat Unit Ref 1 HuRef 1 Celera 1 2 2 (bp) S95 3 ac 19 19 19 15624080 15624117 FAM 1 220~250 S115 gt 16 16 16 19281328 19281359 FAM 1 286~326 S10 aata 13 12 12 20125094 20125145 FAM 1 408~470 S96 ag 18 18 18 15707856 15707891 VIC 1 270~312 S63 ac 17 17 17 7013203 7013236 VIC 1 362~403 S31 ac 22 20 22 7614787 7614830 VIC 1 430~470 DYS602 agat 11 11 11 17274833 17275076 NED 1 228~268 S73 tg 19 19 19 8564199 8564236 NED 1 278~312 S16 agat 11 10 11 15784358 15784401 NED 1 372~424 S85 ctt 25 25 25 14262033 14262107 PED 1 224~290 S6 tacta 12 11 11 20079883 20079942 PED 1 280~336 S20 gata 10 9 10 7475625 7475664 PED 1 390~420 S120 tg 17 17 17 20177037 20177070 PED 1 470~490 S110 tg 17 17 17 17195022 17195055 FAM 2 180~228 DYS390 agat 11 11 11 17274885 17275099 FAM 2 264~308 S32 tg 21 20 21 16171405 16171446 FAM 2 300~360 S4 aag 23 7 23 22170997 22171065 FAM 2 398~440 S70 ctt 22 22 22 8486378 8486443 VIC 2 178~232 DYS391 tcta 11 11 11 14102758 14103044 VIC 2 270~310 S14 tatc 12 11 11 10131987 10132034 VIC 2 372~428 DYS389 gata 12 12 12 14612070 14612436 VIC 2 450~520 S91 gt 16 16 16 15118068 15118099 NED 2 294~332 S5 tccctt 11 8 11 7724423 7724488 NED 2 470~508 S99 ca 20 20 20 15786876 15786915 PED 2 220~264 S64 gt 19 19 19 7492245 7492282 PED 2 270~288 DYS448a19 agagat 11 11 11 24365000 24365293 PED 2 300~390 S13 atag 12 11 12 15809316 15809363 PED 2 390~432 S23 aat 12 13 12 17558865 17558900 PED 2 460~490 S109 ac 16 16 16 17152787 17152818 FAM 3 184~228 S76 ga 18 18 18 8807697 8807732 FAM 3 220~260 S11 aaag 11 14 11 16433141 16433184 FAM 3 310~360 S75 ca 19 19 19 8785760 8785797 FAM 3 388~428 S92 gt 19 19 19 15156043 15156080 FAM 3 420~446 S74 ac 22 22 22 8694382 8694425 VIC 3 260~298 S81 gt 22 22 22 10399276 10399319 VIC 3 370~394 S62 tttc 17 17 17 6921231 6921298 VIC 3 412~476 S105 ac 22 22 22 16350723 16350766 NED 3 208~272 S112 ag 26 26 26 17313869 17313920 NED 3 360~390 S117 ac 22 22 22 19577547 19577590 NED 3 460~484 S80 tg 22 22 22 10250890 10250933 PED 3 224~298 S56 tttc 18 18 18 5469729 5469800 PED 3 420~468 1 3 (Ref HuRef Celera ) ; 2 NCBI GRCh37 Y ; 3 S 36

11 : Y 36 STR 1413 图 1 41 个 Y-STR 位点毛细管电泳分型检测峰图 A 1 13 Y-STR ; B 2 15 Y-STR ; C 3 13 Y-STR

1414 HEREDITAS (Beijing) 2012 34 图 2 41 个 Y-STR 位点的男性个体区分度 (PDM) 图 3 200 个随机上海男性样本的 Network 分析图 ( ) 41 Y-STR, Network 16 (16 4), 9 (P003 P008 P019 P037 P044 P047 P053 P058 P069), 5, 7 (135 294 498 608 626 662 741) ( 4, 5) 6 (P008 P019 P037 P053 P044 P069), 2~4, P003 P047, 6, P058, 135,, Y-STR 3 Y DNA,

11 : Y 36 STR 1415 图 4 姜氏家系样本的遗传亲缘关系图 图 5 姜氏家系亲缘关系密切样本之间的亲缘关系图 Y-STR, 3 NCBI DYS, 48, 200, 100% 41 Y-STR, 36 Y-STR 36 Y-STR 5 DYS,, 200,,,,, Y-STR Y, STR, Y,, SNP Y STR PCR, PCR,, 5,, PCR,,,, 36 Y-STR, 5 DYS 3 PCR, 200 9, 41 STR,, 1~4 (www.chinagene.cn) 参考文献 (References): [1] Painter TS. The Y-Chromosome in mammals. Science, 1921, 27: 503 504.DOI [2] Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS,

1416 HEREDITAS (Beijing) 2012 34 Hillier L, Brown LG, Repping S, Pyntikova T, Ali J, Bieri T, Chinwalla A, Delehaunty A, Delehaunty K, Du H, Fewell G, Fulton L, Fulton R, Graves T, Hou SF, Latrielle P, Leonard S, Mardis E, Maupin R, McPherson J, Miner T, Nash W, Nguyen C, Ozersky P, Pepin K, Rock S, Rohlfing T, Scott K, Schultz B, Strong C, Tin-Wollam A, Yang SP, Waterston RH, Wilson RK, Rozen S, Page DC. The malespecific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature, 2003, 423(6942): 825 837. DOI [3] Garrigan D, Hammer MF. Reconstructing human origins in the genomic era. Nat Rev Genet, 2006, 7(9): 669 680. [4] Cavalli-Sforza LL. The human genome diversity project: past, present and future. Nat Rev Genet, 2005, 6(4): 333 340. DOI [5] Kayser M, Kittler R, Erler A, Hedman M, Lee AC, Mohyuddin A, Mehdi SQ, Rosser Z, Stoneking M, Jobling MA, Sajantila A, Tyler Smith C. A comprehensive survey of human Y-chromosomal microsatellites. Am J Hum Genet, 2004, 74(6): 1183 1197. DOI [6],,,. 22 Y-STR., 2008, 30 (9): 1136 1142. DOI [7],,,. 11 Y-STR., 2008, 30(5): 583 589. DOI [8],,,. 4 17 Y-STR., 2009, 31(9): 921 935. DOI [9],,,,. 7 Y-STR., 2007, 29(10): 1214 1222. DOI [10],,,,,,. Y STR., 2006, 28(12): 1495 1499. DOI [11],,,,. 2 Y-STR., 2006, 28(11): 1355 1360. DOI [12] Grsković B, Mrsić G. Y chromosome: from evolution to forensics- an overview. Acta Med Croatica, 2010, 64(1): 33 40. DOI [13] McElreavey K, Quintana-Murci L. A population genetics perspective of the Indus valley through uniparentallyinherited markers. Ann Hum Biol, 2005, 32(2): 154 162. DOI [14] Gemmell NJ, Sin FY. Mitochondrial mutations may drive Y chromosome evolution. Bioessays, 2002, 24(3): 275 279. DOI [15] Kayser M, Roewer L, Hedman M, Henke L, Henke J, Brauer S, Krüger C, Krawczak M, Nagy M, Dobosz T, Szibor R, de Knijff P, Stoneking M, Sajantila A. Charaeteristics and frequency of germline mutations at microsatellite loci from the human Y chromosome, as revealed by direct observation in father/son Pairs. Am J Hum Genet, 2000, 66(5): 1580 1588. DOI [16],,. 17 Y-STR., 2011, 33(3): 228 238. DOI [17] Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B, DNA recommendations. Further report of the DNA Commission of the ISFG regarding the use of short tandem repeat systems. Forensic Sci Int, 1997, 87(3): 179 184. DOI [18] Pericić M, Barać Lauc L, Martinović Klarić I, Janićijević B, Rudan P. Review of Croatian genetic heritage as revealed by mitochondrial DNA and Y chromosomal lineages. Croat Med J, 2005, 46(4): 502 513. DOI [19] Scheinfeldt L, Friedlaender F, Friedlaender J, Latham K, Koki G, Karafet T, Hammer M, Lorenz J. Unexpected NRY chromosome variation in Northern Island melanesia. Mol Biol Evol, 2006, 23(8): 1628 1641. [20] Bandelt HJ, Forster P, Röhl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol, 1999, 16(1): 37 48. DOI

11 : Y 36 STR 1417 附表 1 133 个验证 STR 位点信息 STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 DYS19 tatc 12 12 12 9521935 9522128 DYS197 tg 11 11 11 18468760 18468890 DYS199 ca 13 13 13 19096303 19096543 DYS209 tg 18 18 18 20932050 20932311 DYS211 caa 11 11 11 20975104 20975294 DYS257 tg 14 14 14 8620197 8620484 DYS274 gt 11 11 11 14637813 14638181 DYS280 tg 11 11 11 16543938 16544240 DYS288 ac 15 15 15 7657608 7657726 DYS289 ac 7 12 7 18821148 18821299 DYS379 tg 12 12 12 24631231 24631480 DYS385 gaaa 14 14 14 20842336 20842716 DYS389 gata 12 12 12 14612070 14612436 DYS390 agat 19 19 19 17274885 17275099 DYS391 tcta 11 11 11 14102758 14103044 DYS392 aat 13 13 13 22633758 22634011 DYS393 tctct 14 14 14 3131458 3131528 DYS395 agat 12 12 12 3131128 3131244 DYS413 ac 23 23 23 16167253 16167426 DYS426 tgt 12 12 12 19134813 19134909 DYS437 tcta 10 16 16 14466994 14467058 DYS438 ttttc 10 10 10 14937824 14937874 DYS446a13 tc 38 38 38 3131434 3131545 DYS447a22 ttata 5 5 5 15278692 15278902 DYS448 agagat 24 24 24 24365010 24365246 DYS448a19 agagat 11 11 11 24365000 24365293 DYS458 tatc 17 16 16 9521988 9522036 DYS463a18 ggaag 17 17 17 7643407 7643660 DYS602 agat 11 11 11 17274833 17275076 DYS80 agat 11 11 11 18957359 18958848 S1 gaaag 17 7 17 9090409 9090493 S10 aata 13 12 12 20125094 20125145 S100 at 20 20 20 15950570 15950609 S101 ta 18 18 18 16036833 16036868 S105 ac 22 22 22 16350723 16350766 S106 at 22 22 22 16526503 16526546 S107 at 19 19 19 16559443 16559480 S108 tg 16 16 16 16929108 16929139 S109 ac 16 16 16 17152787 17152818 S11 aaag 11 14 11 16433141 16433184 S110 tg 17 17 17 17195022 17195055 S111 tc 27 27 27 17269637 17269690 S112 ag 26 26 26 17313869 17313920 S115 gt 16 16 16 19281328 19281359

1418 HEREDITAS (Beijing) 2012 34 1 STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 S116 tg 17 17 17 19522151 19522184 S117 ac 22 22 22 19577547 19577590 S118 ac 19 19 19 20082909 20082946 S119 tg 16 16 16 20102245 20102276 S12 tatc 12 13 12 15951178 15951225 S120 tg 17 17 17 20177037 20177070 S124 gt 17 17 17 22308790 22308823 S125 tg 17 17 17 22391222 22391255 S127 at 19 19 19 26991019 26991056 S128 ta 17 17 17 27110120 27110153 S13 atag 12 11 12 15809316 15809363 S14 tatc 12 11 11 10131987 10132034 S15 aaat 11 12 11 8526195 8526238 S16 agat 11 10 11 15784358 15784401 S17 ttta 11 12 11 7378491 7378534 S18 atcc 11 11 10 19820653 19820696 S19 tgtt 10 9 9 7774985 7775024 S2 ac 17 17 11 6209872 6209905 S20 gata 10 9 10 7475625 7475664 S21 tcta 10 12 10 14752027 14752066 S22 tct 18 17 17 8561690 8561743 S23 aat 12 13 12 17558865 17558900 S24 gga 10 8 12 5453363 5453392 S25 ta 31 26 26 22825107 22825168 S26 at 29 30 29 12590036 12590093 S27 ta 28 27 28 7592277 7592332 S28 at 27 23 27 5096584 5096637 S29 ta 27 24 27 14873453 14873506 S3 ta 19 6 8 6210010 6210047 S30 ta 23 24 23 17829229 17829274 S31 ac 22 20 22 7614787 7614830 S32 tg 21 20 21 16171405 16171446 S33 ta 20 20 18 21702987 21703026 S34 at 19 21 19 20225922 20225959 S35 at 19 20 20 17818817 17818854 S36 ta 17 16 17 16325458 16325491 S37 ac 15 16 15 13110831 13110860 S38 ta 15 16 16 19264274 19264303 S39 ta 15 16 15 12939414 12939443 S4 aag 23 7 23 22170997 22171065 S40 tg 12 7 12 13104540 13104563 S41 ct 12 9 12 7678066 7678089 S42 ac 12 13 12 8682200 8682223 S43 gt 11 7 11 16314918 16314939 S44 tg 10 9 10 7304054 7304073

11 : Y 36 STR 1419 1 STR# Repeat Unit Ref 1 HuRef 1 Celera 1 5 POS. 2 3 POS. 2 S46 ta 6 7 6 11963600 11963611 S48 ta 20 20 20 2867025 2867064 S49 at 26 26 26 3882963 3883014 S5 tccctt 11 8 11 7724423 7724488 S52 tg 16 16 16 5246755 5246786 S53 gt 19 19 19 5279337 5279374 S54 ac 16 16 16 5301787 5301818 S55 at 16 16 16 5414714 5414745 S56 tttc 18 18 18 5469729 5469800 S57 tg 17 17 17 5562828 5562861 S6 tacta 12 11 11 20079883 20079942 S60 ta 21 21 21 6406083 6406124 S61 ct 16 16 16 6655213 6655244 S62 tttc 17 17 17 6921231 6921298 S63 ac 17 17 17 7013203 7013236 S64 gt 19 19 19 7492245 7492282 S67 ac 16 16 16 7671641 7671672 S68 ac 17 17 17 7731308 7731341 S70 ctt 22 22 22 8486378 8486443 S73 tg 19 19 19 8564199 8564236 S74 ac 22 22 22 8694382 8694425 S75 ca 19 19 19 8785760 8785797 S76 ga 18 18 18 8807697 8807732 S77 tg 17 17 17 8834717 8834750 S79 ta 21 21 21 10134020 10134061 S8 cttt 15 16 15 16902370 16902429 S80 tg 22 22 22 10250890 10250933 S81 gt 22 22 22 10399276 10399319 S82 gt 16 16 16 10433411 10433442 S83 at 25 25 25 11789880 11789929 S85 ctt 25 25 25 14262033 14262107 S86 at 18 18 18 14419149 14419184 S89 ca 19 19 19 14917509 14917546 S9 tttc 14 13 14 14572566 14572621 S90 tg 18 18 18 15023699 15023734 S91 gt 16 16 16 15118068 15118099 S92 gt 19 19 19 15156043 15156080 S93 ta 21 21 21 15328366 15328407 S94 ac 17 17 17 15343050 15343083 S95 ac 19 19 19 15624080 15624117 S96 ag 18 18 18 15707856 15707891 S97 ta 17 17 17 15748685 15748718 S98 ac 18 18 18 15752373 15752408 S99 ca 20 20 20 15786876 15786915 1 3 (Ref, HuRef, Celera) ; 2 NCBI GRCh37 Y

1420 HEREDITAS (Beijing) 2012 34 附表 2 41 个 Y-STR 引物信息 (5-3 ) 1 S95F CTTTCGGCCCAGTAAGGGTATTCAAGGCTGCTAGTCATGCTTCTA 1 S95R GTTTGCTTTGAAGCGTGTTTGAAAGAA 1 S115F CTTTCGGCCCAGTAAGGGTATGGTCAACAGAAGGGCTTGTTATTTTC 1 S115R GTTTAGCCCCCTCAATTAGCCTTTTG 1 S10F CTTTCGGCCCAGTAAGGGTATCCTCCCCACATTCTGAAATCAC 1 S10R GTTTCCAAGAGATTCAGAGGCCTCCAC 1 S96F TAGACTAAACGGGCGTGGTTCTGCTGGTGCCTGATGACAGA 1 S96R GTTTGGCCCTGGGGAAAATAAGATCA 1 S63F TAGACTAAACGGGCGTGGTTCGCAGGAGAGCAATGCCTTGTAA 1 S63R GTTTtgatggagatgggttgctgaag 1 DYS602F ATGAGACTACGCGGCTAATGCTGAGTGGGAGAAATGGATGACA 1 DYS602R GTTTTTTTTGGGCCCTGCATTTTG 1 S31F TAGACTAAACGGGCGTGGTTCTGTCTGATGATGCCCAAAATGA 1 S31R GTTTGGAAAAACAGACCCAGTCACAAA 1 S73F ATGAGACTACGCGGCTAATGCCTCAAGAGCTGCCAGGCTGTGT 1 S73R GTTTGCCTGCCTCCCAATTGACTAAA 1 S16F ATGAGACTACGCGGCTAATGCACAGTGTATCCGCCATGGTAGC 1 S16R GTTTGGGATGGGAAATGATGTTTCTGA 1 S85F GTGTTCCGACCGCTATTGTTTGACTCCCCCATGCCAGTAAGAA 1 S85R GTTTGGGAACTGAGGGAAGGCAAAA 1 S6F GTGTTCCGACCGCTATTGTTTGGGGCCTTACTTACATTGGTGGT 1 S6R GTTTTGGGTGCTAAATGCCCAAGAGT 1 S20F GTGTTCCGACCGCTATTGTTTTCCACAATGCTTTCCTTACACCTG 1 S20R GTTTAACCAGTGAGAGCCGGAACCTC 1 S120F GTGTTCCGACCGCTATTGTTTCAAACAGGCAGGCTCCTCCTAA 1 S120R GTTTaggATTTCCCTCACCATTTTCTG 2 S110F CTTTCGGCCCAGTAAGGGTATCCCCGGGCTTATTTCAGATTTA 2 S110R GTTTTGTGAGCCAGCCACACTTGATA 2 DYS390F CTTTCGGCCCAGTAAGGGTATACAGTGTATCCGCCATGGTAGC 2 DYS390R GTTTCATTTTTGGGCCCTGCATTT 2 S32F CTTTCGGCCCAGTAAGGGTATCAGCAGGGAGGACTTGGACAAA 2 S32R GTTTgctttcttccaaccatccattca 2 S4F CTTTCGGCCCAGTAAGGGTATTTTGAAAGCTGGTGGGATTCAG 2 S4R GTTTAGCAGCTGCCAGGTTGTGAA 2 S70F TAGACTAAACGGGCGTGGTTCAGGAATGTGGCTAACGCTGTTCA 2 S70R GTTTAATCAGAACACAGAGCCCCACAA 2 DYS391F TAGACTAAACGGGCGTGGTTCccccaccacagattagcattca 2 DYS391R GTTTagggataggtaggcaggcagat 2 S14F TAGACTAAACGGGCGTGGTTCTCAGGAATTTGCTGGTCAATCTC 2 S14R GTTTGGAAAATTTTGACAAGCCCAAAG 2 DYS389F TAGACTAAACGGGCGTGGTTCGAATGTCatagatagatgatggactgc 2 DYS389R GTTTttgCATTAGCATGAGAGATCctg

11 : Y 36 STR 1421 2 (5-3 ) 2 S91F ATGAGACTACGCGGCTAATGCCCTCACAGGAAGCAGAATTCACTACA 2 S91R GTTTTCTTTGTTTCAAGTTGGCCaattt 2 S5F ATGAGACTACGCGGCTAATGCTCCCTAAAAGTTACAACCCAGCAC 2 S5R GTTTCACAGCAGTggctaggcacag 2 S99F GTGTTCCGACCGCTATTGTTTATGGTCCCTCTTAATGTTGCCTGT 2 S99R GTTTTCTTTTGTCTGGGGTATGACAGCTA 2 S64F GTGTTCCGACCGCTATTGTTTAATGCATTAACAGCCCATCCCATTAG 2 S64R GTTTgaaggaaccagcacttcccata 2 DYS448a19F GTTTAGAAATAGAGATCGCgagacagaaag 2 DYS448a19R GTGTTCCGACCGCTATTGTTTTGATTCCCTGTGTTGGAGACCTTTTC 2 S13F GTTTcctcccttacCACAGATAGGTAAGACA 2 S13R GTGTTCCGACCGCTATTGTTTTGAAGATGTTCAGTCAGGAAAAAAAA 2 S23F GTGTTCCGACCGCTATTGTTTCGCATGGACTTCCACCCACTTA 2 S23R GTTTTTCCAAGCCGTTAATTTCCTCAT 3 S109F CTTTCGGCCCAGTAAGGGTATAAGCTGTTACCACCTGCTTACATAGAA 3 S109R GTTTGAGATGAAGCTTCTTTGAGGCATAA 3 S76F GTTTGGGTTCACTCTAGGTGGACAGAAAA 3 S76R CTTTCGGCCCAGTAAGGGTATTCACAGGGGTCCAAAGGAAAC 3 S11F GTTTcctacctgggcaacagaacaaca 3 S11R CTTTCGGCCCAGTAAGGGTATCCCAGACAGAATGCAATCTTCttt 3 S75F GTTTCATCATTCTATATGAAGCAGTGCAAAA 3 S75R CTTTCGGCCCAGTAAGGGTATGTGATAAAGGCTTGGCAAAAAGAGA 3 S92F CTTTCGGCCCAGTAAGGGTATTTTGCACCTCTGGTCTTACTGGTC 3 S92R GTTTACCTGCCATCGAAAGCATTACC 3 S74F TAGACTAAACGGGCGTGGTTCGATCCCACAGCCCAAGCAGAT 3 S74R GTTTGGGACTTCTGCAAAACCATACCA 3 S62F TAGACTAAACGGGCGTGGTTCGCCTGGGACCGTGTCTTTATTT 3 S62R GTTTGGTGGCAACCTAAGCTGAAATG 3 S81F TAGACTAAACGGGCGTGGTTCGGGCCTGGCAATCTTTTCAC 3 S81R GTTTTGACCCTTTCCACAGACCCTTAAC 3 S105F ATGAGACTACGCGGCTAATGCGGCCATTCAGTTCTTCATCAGCTC 3 S105R GTTTTGATGAGAAACCACCACTGTAACTAGG 3 S112F ATGAGACTACGCGGCTAATGCTGACCAAAGCAAATCCTGAAATATG 3 S112R GTTTTCCCCAAAGGCAACCAAGAA 3 S117F ATGAGACTACGCGGCTAATGCTCTGCCAACCCACCTGATGATA 3 S117R GTTTTGACtgatttgctcacaatcacatatc 3 S80F GTGTTCCGACCGCTATTGTTTAGGCCCAATTTTTTGCACACAG 3 S80R GTTTGGCTGAAGTGCATTAGCCACATT 3 S56F GTGTTCCGACCGCTATTGTTTCATGTGCAAGGCAAAAGTACCTC 3 S56R GTTTCAGTTGTGGTACAGGCTGGATTT

1422 HEREDITAS (Beijing) 2012 34 附表 3 200 个上海随机男性样本单倍型信息 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S16 5 6 6 4 4 3 3 6 2 1 3 7 3 4 3 3 5 6 5 6 5 4 4 3 6 1 2 1 3 8 3 4 5 3 7 4 5 6 5 4 4 3 5 1 2 1 3 7 3 4 5 3 7 4 5 6 5 4 4 3 6 1 2 1 3 8 3 4 5 3 7 4 5 4 6 1 4 3 6 6 7 1 4 10 7 4 5 2 3 4 5 6 4 6 2 3 5 6 2 1 3 3 9 2 2 3 5 5 5 4 4 7 4 3 4 6 4 1 3 3 7 3 4 3 5 4 3 6 4 7 4 6 3 6 4 1 2 7 4 3 4 2 5 6 6 6 4 7 4 3 5 6 4 1 5 9 3 3 5 3 5 4 3 6 4 7 4 6 3 6 4 1 2 5 6 3 4 2 5 6 3 6 4 7 4 6 3 6 4 1 2 7 6 3 4 2 5 5 5 4 4 7 4 3 4 7 4 1 3 3 5 3 4 3 5 3 5 6 5 4 5 3 1 7 5 1 3 7 1 4 5 3 5 5 5 5 4 7 4 3 4 6 4 1 3 8 3 3 5 4 4 4 5 6 4 10 4 3 4 7 2 1 3 8 3 4 5 3 8 5 5 5 6 4 4 3 5 4 5 1 3 6 4 4 5 3 3 4 5 5 6 4 4 3 5 6 5 1 3 8 5 4 5 3 3 4 5 6 4 7 2 3 6 6 2 1 3 3 6 2 2 3 5 4 5 9 4 7 4 3 5 6 2 1 4 8 7 2 2 3 2 6 5 4 4 7 4 3 4 6 4 1 3 8 4 3 5 4 6 4 5 6 4 13 4 3 4 6 4 1 3 7 3 4 5 3 8 5 5 7 7 3 4 3 6 1 2 1 4 7 4 4 5 3 5 7 5 7 8 4 4 3 5 6 2 1 3 9 4 4 5 3 7 5 5 9 5 6 4 3 5 6 3 1 3 4 6 4 5 3 5 5 5 6 7 4 4 3 7 1 2 1 3 7 4 4 5 3 5 5 5 6 5 6 4 3 6 6 4 1 4 9 2 4 6 3 5 5 5 3 4 7 4 3 4 6 4 1 4 2 4 3 5 3 5 4 5 3 4 7 4 3 5 6 4 1 3 2 6 3 5 3 5 4 5 4 4 7 4 3 4 6 4 1 3 5 4 3 4 3 5 4 5 4 4 7 4 3 4 6 4 1 4 5 5 3 4 3 5 4 5 4 3 7 4 3 4 6 4 1 3 2 6 3 4 3 5 4 5 4 4 7 4 3 4 6 4 1 3 4 5 3 4 3 5 4 5 4 4 7 4 3 4 6 4 1 3 4 7 3 4 3 5 4 5 3 4 7 4 3 4 6 4 1 4 6 4 3 4 3 5 4 5 6 7 3 4 3 6 6 2 1 3 8 3 4 5 3 5 6 3 5 8 4 4 3 7 6 2 1 3 8 5 4 5 3 7 5 5 5 7 4 5 3 6 6 2 1 3 7 3 4 5 3 5 5 5 6 6 4 4 3 6 6 2 1 3 7 3 4 5 3 6 6

11 : Y 36 STR 1423 3 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S16 5 6 7 4 4 3 6 6 2 1 3 7 5 4 5 3 5 6 5 7 7 4 4 3 6 6 2 1 3 9 2 4 5 3 5 6 5 7 8 4 4 3 7 6 2 1 3 7 5 4 5 3 7 5 5 7 8 4 4 3 7 6 2 1 3 7 5 4 5 3 7 5 5 8 7 4 4 3 6 1 2 1 3 7 4 4 5 3 5 6 5 8 8 4 4 3 7 6 2 1 3 7 4 4 5 3 5 6 5 5 6 4 4 3 5 6 5 1 4 9 4 4 5 3 3 4 5 6 8 4 4 3 6 1 2 1 3 9 5 4 5 3 5 6 5 5 7 4 4 3 5 1 2 1 2 9 3 4 5 3 5 7 5 5 7 4 4 3 5 1 2 1 3 9 4 4 5 3 5 6 5 7 5 6 4 3 5 8 4 1 3 8 4 4 6 3 5 5 6 7 4 7 4 3 5 6 4 1 4 8 5 3 6 3 5 5 6 7 4 7 4 3 6 6 4 1 5 10 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 11 5 3 5 3 5 4 5 5 4 9 4 3 4 6 4 1 3 8 4 4 5 3 7 4 5 6 4 9 4 3 4 5 4 1 3 9 5 4 5 3 8 6 3 7 4 7 4 6 3 5 4 1 1 5 5 3 4 2 5 5 5 4 4 6 4 3 4 6 4 1 3 7 6 3 4 3 5 5 5 3 4 7 4 3 5 6 4 1 3 2 4 3 5 3 5 4 5 3 4 7 4 3 5 6 4 1 3 2 5 3 5 3 5 4 6 6 4 7 4 3 4 6 4 1 4 5 4 3 5 3 5 5 5 6 4 7 4 5 6 4 4 1 4 8 7 3 4 5 3 7 6 6 4 7 4 3 5 6 4 1 4 11 5 3 5 3 5 4 6 8 4 7 4 3 5 6 4 1 3 11 5 3 5 3 5 4 5 6 7 2 4 3 7 5 2 1 3 9 3 4 5 3 5 6 5 6 7 4 4 3 6 1 2 1 3 9 4 4 5 3 6 6 5 7 7 4 4 3 6 1 2 1 3 10 3 4 5 3 5 7 5 7 7 4 4 3 7 1 2 1 3 9 1 4 5 3 5 6 5 7 7 4 5 3 7 6 2 1 2 7 2 5 5 3 5 5 5 9 7 4 5 3 6 6 2 1 3 5 3 4 5 3 5 6 6 6 7 4 5 3 6 6 2 1 3 8 4 4 5 3 5 6 5 4 4 7 4 3 4 6 4 1 4 4 8 3 4 3 5 4 6 6 4 7 4 3 5 6 4 1 4 10 5 3 5 3 5 4 5 5 5 6 4 3 5 7 4 1 3 5 7 2 5 3 5 3 5 6 7 4 5 3 5 6 2 1 2 7 3 4 5 3 5 5 5 4 5 7 4 3 4 4 4 1 3 2 5 3 5 3 5 4 5 5 7 4 4 3 5 6 2 1 3 7 5 4 5 3 3 5 6 5 7 4 5 3 7 6 2 1 3 9 4 4 5 3 5 7

1424 HEREDITAS (Beijing) 2012 34 3 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S16 5 5 7 4 5 3 5 6 2 1 3 7 3 4 5 3 5 6 5 6 5 4 4 2 5 8 4 1 4 8 7 5 4 3 5 6 5 6 5 4 4 3 5 6 4 1 2 7 3 5 4 3 5 6 5 7 5 4 4 3 5 5 4 1 2 7 7 5 4 3 5 6 5 6 5 7 2 3 6 6 2 1 3 4 7 2 2 3 5 4 5 4 5 6 4 3 5 6 4 1 3 9 5 4 5 3 6 4 5 9 4 6 4 3 7 6 2 1 3 8 6 3 5 3 5 4 5 5 4 7 2 3 6 5 1 1 3 6 6 2 2 3 5 4 5 5 4 7 2 3 6 6 2 1 3 4 7 2 2 3 5 4 5 6 4 7 2 3 6 6 2 1 3 4 7 2 1 3 5 4 5 6 4 7 2 3 6 6 2 1 3 4 7 2 2 3 5 4 5 6 4 7 2 3 6 6 2 1 3 6 5 2 2 3 5 4 5 7 4 7 2 3 5 6 2 1 4 5 7 2 2 3 5 4 5 7 4 7 2 3 6 6 2 1 4 5 6 2 2 3 5 4 5 9 4 7 2 3 5 6 2 1 3 5 7 2 2 3 5 4 6 5 4 6 2 3 6 6 2 1 3 5 6 2 2 3 5 4 6 7 4 7 2 3 5 6 2 1 4 5 7 2 2 3 5 4 1 3 5 6 4 3 6 6 4 1 3 7 6 4 5 3 6 4 5 4 5 6 4 3 5 6 4 1 3 7 4 4 5 3 6 4 5 4 5 6 4 3 5 6 4 1 3 7 6 4 5 3 8 4 5 6 4 7 2 3 5 6 2 1 3 4 9 2 2 3 5 4 5 9 4 7 2 3 5 3 2 1 4 7 8 2 2 3 5 4 5 6 4 7 2 3 5 5 3 1 3 3 6 2 2 3 5 4 5 4 5 6 4 3 5 6 4 1 3 7 5 4 5 3 6 4 5 5 5 6 4 3 5 6 4 1 3 6 6 4 5 3 6 4 2 5 7 4 5 3 5 6 2 1 3 8 3 4 5 3 5 3 5 10 4 6 4 3 2 6 4 1 4 8 6 4 5 3 9 5 5 7 7 4 4 3 6 1 2 1 2 6 3 4 5 3 5 5 5 8 7 4 4 3 6 1 3 1 3 9 3 4 5 3 5 6 5 9 7 4 4 4 5 1 3 1 3 8 3 4 5 3 5 6 5 4 5 6 4 3 5 7 4 1 3 8 5 4 5 3 6 3 6 7 4 7 4 3 5 6 4 1 4 9 5 3 5 3 6 4 6 7 4 12 4 3 5 6 4 1 3 8 5 3 5 3 5 4 5 4 5 6 4 3 5 6 4 1 3 9 4 4 5 3 6 4 6 8 4 7 4 3 5 6 4 1 3 11 3 3 5 3 6 4 5 5 4 9 4 3 4 6 4 1 4 8 4 4 5 3 7 5 6 6 4 7 4 3 5 6 2 1 4 10 5 3 5 3 5 4 6 6 4 7 4 3 5 6 4 1 3 9 2 3 5 3 5 4 6 7 4 5 4 3 5 6 4 1 3 9 4 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 11 6 3 5 3 5 4 6 9 4 7 4 3 5 5 4 1 5 10 5 3 5 3 5 4 6 9 4 7 4 3 5 6 4 1 3 9 5 3 5 3 5 4

11 : Y 36 STR 1425 3 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S16 5 5 4 9 4 3 5 6 4 1 3 11 4 4 5 3 8 4 5 5 5 6 4 5 5 6 4 1 3 6 5 5 5 3 6 4 5 6 4 9 4 3 4 6 4 1 3 7 4 4 5 3 6 6 5 7 4 6 4 3 5 6 3 1 3 5 5 4 5 3 5 5 6 7 4 7 4 3 5 6 4 1 3 10 6 3 5 3 5 4 6 7 4 7 4 3 5 6 4 2 4 8 5 3 5 3 5 4 7 6 4 7 4 3 5 6 4 1 3 8 7 3 5 3 5 4 7 5 4 7 4 3 5 6 4 1 3 10 7 3 5 3 5 4 5 4 4 9 4 3 7 6 4 1 3 7 6 4 5 3 9 5 5 6 4 9 4 3 4 6 4 1 5 7 4 4 5 3 8 6 5 6 5 6 4 3 5 6 4 1 3 8 3 4 5 3 6 4 5 6 4 7 2 3 6 6 2 1 3 8 7 2 2 3 5 4 5 8 4 7 2 3 5 6 2 1 5 7 4 2 4 3 5 5 5 7 4 7 2 3 6 6 2 1 4 8 6 2 2 3 5 4 5 3 4 7 4 3 4 6 4 1 3 4 6 3 4 3 5 5 6 5 4 7 4 3 4 6 4 1 4 3 4 3 5 3 5 5 5 6 7 4 3 3 7 6 2 1 3 9 4 4 5 3 5 5 5 5 5 6 4 3 5 6 4 1 3 5 7 4 5 3 6 4 5 9 4 6 4 3 2 6 4 1 4 8 8 4 5 3 9 6 5 9 5 6 4 3 5 5 4 1 3 6 4 4 5 3 5 6 5 6 7 4 4 3 6 6 2 1 3 9 5 4 5 3 5 6 6 7 4 5 4 3 5 6 3 1 4 8 5 3 5 3 5 4 5 3 5 5 5 3 5 8 4 1 3 6 4 4 5 3 6 4 5 4 5 6 4 3 5 5 4 1 3 8 6 4 5 3 6 4 5 6 5 6 4 3 5 6 4 1 3 7 3 4 5 3 4 4 5 9 4 7 2 3 5 2 2 1 3 6 5 2 2 3 5 4 6 8 4 7 4 3 5 6 4 1 3 7 5 3 5 3 5 4 6 7 4 7 4 3 7 6 4 1 3 9 6 3 5 3 5 4 6 8 4 7 4 1 5 6 4 1 3 10 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 4 9 5 3 5 3 5 4 6 8 4 7 4 3 5 6 4 1 4 9 5 3 5 3 5 4 5 5 4 7 4 3 5 6 4 1 4 10 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 4 10 4 3 5 3 5 4 6 7 4 7 4 3 6 6 4 1 4 10 5 3 5 3 5 4 6 9 4 7 4 3 6 6 4 1 4 10 4 3 5 3 5 5 6 6 4 7 4 3 7 6 4 1 5 11 6 3 5 3 5 4 3 4 4 9 4 3 4 6 6 1 3 7 4 4 5 3 5 5 5 5 4 9 4 3 4 6 4 1 3 10 4 4 5 3 8 4 5 5 4 9 4 3 4 6 7 1 2 8 2 4 6 3 8 6 6 5 4 9 4 3 4 6 4 1 3 9 3 4 5 3 8 5 5 4 4 9 4 3 5 6 4 1 3 7 3 4 5 3 8 5 5 5 4 9 4 3 5 6 4 1 3 8 5 4 5 3 8 5

1426 HEREDITAS (Beijing) 2012 34 3 S5 S70 S73 S74 S75 S76 S14 S80 S81 S40 DYS391 S85 DYS389 S91 S92 S95 S96 S16 6 7 4 7 4 3 5 6 4 1 3 9 6 3 5 3 5 4 6 10 4 7 4 3 5 6 4 1 3 10 4 3 5 3 6 4 5 7 4 4 4 3 5 6 5 1 3 7 5 4 5 3 3 5 5 8 4 7 2 3 5 6 2 1 3 5 8 2 2 3 5 5 5 8 4 7 4 3 7 4 2 1 2 8 4 2 2 3 5 6 5 5 4 9 4 3 4 6 4 1 4 7 5 4 5 3 8 3 6 5 4 7 4 3 5 6 4 1 4 10 4 3 5 3 5 4 6 6 4 6 4 3 5 6 4 1 4 8 5 3 5 3 5 4 6 6 4 7 4 3 5 6 4 1 3 7 3 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 5 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 9 6 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 10 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 3 10 5 3 5 3 5 5 6 7 4 7 4 3 5 6 4 1 4 8 5 3 5 3 5 4 6 8 5 7 4 3 5 6 4 1 4 10 4 3 5 3 5 4 5 5 4 11 4 3 5 6 4 1 4 9 4 4 5 3 8 5 5 5 4 6 4 3 4 6 4 1 4 8 3 5 3 3 6 6 5 4 4 9 4 3 4 6 4 1 3 9 2 4 5 3 8 4 5 5 4 9 4 3 4 6 2 1 3 6 5 4 5 3 8 5 5 5 4 9 4 3 4 6 4 1 3 11 5 4 5 3 8 4 6 3 4 8 4 3 6 6 4 1 4 9 3 3 5 3 5 4 3 5 4 9 4 3 4 6 4 1 3 8 3 4 5 3 8 6 5 5 4 11 2 3 4 6 5 1 3 8 3 4 5 3 8 5 5 5 5 6 5 3 5 6 4 1 3 9 3 4 6 3 6 4 5 7 5 6 4 3 4 6 4 1 3 9 6 4 5 3 5 6 5 6 7 4 4 3 7 6 2 1 3 7 4 4 5 3 5 6 4 9 4 6 4 3 4 8 4 1 3 9 7 2 6 3 4 5 6 7 4 8 4 3 5 6 4 1 5 10 5 3 5 3 5 4 6 7 4 7 4 3 5 6 4 1 4 10 6 3 5 3 6 4 5 4 4 9 4 3 4 6 4 1 3 9 5 4 5 3 8 4 3 5 5 7 4 3 6 6 2 1 3 6 5 4 6 3 5 5 5 5 5 7 4 4 4 6 4 1 4 6 5 2 6 3 5 5 5 9 4 7 2 3 6 6 2 1 3 4 5 2 2 3 5 4 5 6 7 4 4 3 7 6 2 1 3 8 5 4 5 3 5 7 6 7 4 7 4 3 5 6 4 1 4 10 4 3 5 3 5 4 5 5 5 6 4 3 5 6 4 1 3 8 6 4 5 3 6 4 5 5 5 6 4 3 5 6 4 1 3 8 5 4 7 3 5 5 3 5 4 5 4 3 5 8 4 1 3 6 6 3 5 3 5 5 5 5 5 6 4 3 5 6 4 1 3 4 6 4 5 3 6 5 5 5 5 4 4 3 5 6 4 1 4 11 2 4 5 3 6 5

11 : Y 36 STR 1427 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 4 7 6 5 5 3 3 5 6 5 5 5 4 5 5 4 10 5 3 6 7 4 4 3 3 3 4 3 5 5 4 5 5 4 14 5 3 6 6 4 4 3 3 3 4 3 5 5 4 5 5 4 16 5 3 6 6 4 4 3 3 3 4 3 5 5 4 5 5 4 10 5 5 6 6 5 6 3 3 3 4 5 5 5 5 5 5 4 12 5 3 7 7 6 8 5 3 4 5 6 5 5 4 3 6 3 12 8 5 5 6 5 5 3 2 3 4 6 4 5 4 5 6 3 12 5 5 8 6 5 4 3 2 5 6 10 6 5 3 4 6 3 8 5 4 6 6 3 6 3 5 3 4 4 4 5 5 5 6 3 10 4 5 8 6 5 4 3 2 5 6 10 6 5 3 4 6 3 10 5 5 7 6 4 5 3 2 4 5 9 6 5 3 3 6 3 8 5 5 5 4 5 4 3 2 2 3 6 4 5 4 4 6 3 16 5 5 7 6 4 10 3 3 4 5 6 5 5 4 5 6 4 16 5 5 5 6 5 10 3 3 3 4 6 4 5 4 4 8 3 10 7 5 6 5 4 8 3 3 4 5 5 7 5 4 4 6 4 12 7 5 5 6 5 6 3 3 3 4 8 5 5 5 4 5 4 14 5 5 5 6 5 10 3 3 3 4 8 5 5 5 5 5 4 14 5 3 7 6 6 8 5 3 3 4 6 5 5 4 5 6 2 8 8 2 6 6 6 10 5 3 5 6 4 5 5 4 5 6 2 8 8 5 5 6 5 10 3 3 3 4 6 4 5 4 4 6 3 17 5 5 6 6 5 6 3 3 4 5 5 5 5 4 4 6 4 14 9 3 5 6 5 4 3 3 6 7 5 5 5 4 5 5 4 10 5 3 6 6 5 4 3 3 4 5 5 5 5 6 6 5 4 10 5 4 6 5 5 10 3 3 4 5 7 5 5 4 4 7 4 12 9 3 6 6 6 4 3 3 4 5 5 5 5 4 6 6 4 12 5 5 7 6 5 5 3 4 4 5 11 6 4 3 5 6 4 14 5 5 5 6 5 4 3 3 3 4 6 4 5 4 5 5 3 14 4 5 5 6 5 5 3 3 3 4 6 4 5 4 4 7 3 14 4 5 5 6 5 4 3 2 3 4 6 4 5 4 4 6 3 17 7 5 6 6 5 4 3 2 3 4 6 4 5 4 4 6 3 12 5 5 5 6 6 5 3 2 3 4 6 4 5 4 5 6 3 12 5 4 5 6 6 5 3 2 3 4 4 4 5 4 5 6 3 14 5 5 5 6 5 5 3 2 3 4 6 4 5 4 5 6 3 12 5 5 5 6 5 4 1 2 3 4 6 4 5 4 4 6 3 12 5 3 6 6 5 5 3 3 5 6 3 5 6 4 5 6 4 12 7 3 6 6 5 3 3 3 4 5 5 4 5 4 5 5 4 10 7 3 6 6 5 5 3 3 4 5 5 5 5 4 5 7 4 8 7 3 6 6 5 5 3 3 5 6 5 6 6 4 5 6 4 12 5

1428 HEREDITAS (Beijing) 2012 34 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 3 5 6 5 8 3 3 5 6 5 5 5 4 5 6 4 14 7 3 6 5 5 5 3 3 5 6 5 5 6 4 5 6 4 14 5 3 4 6 5 2 3 3 4 5 5 5 5 4 5 6 4 12 7 3 6 6 5 3 3 3 4 5 5 5 5 4 5 5 4 12 7 3 6 6 5 3 3 3 5 6 5 5 5 4 5 5 4 14 5 3 6 6 5 4 3 3 5 6 3 5 5 4 6 5 4 10 5 5 6 6 5 6 3 3 3 4 6 5 5 4 7 5 4 10 5 3 5 6 5 5 3 3 5 6 7 5 5 4 5 6 4 14 5 3 6 6 5 4 3 3 6 7 5 5 5 4 4 5 4 16 5 3 6 6 5 4 3 3 5 6 5 5 5 4 5 5 4 14 5 5 6 6 5 6 3 5 4 5 10 6 4 3 5 6 4 12 7 4 6 6 3 8 3 4 4 5 4 4 5 5 6 6 3 10 4 4 6 6 3 8 3 4 3 4 4 4 5 5 5 6 3 12 4 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 12 4 5 5 6 5 8 3 4 3 4 5 5 5 4 4 6 4 14 7 5 6 6 5 6 3 3 5 6 3 5 3 4 4 6 4 16 7 5 6 6 5 5 3 2 4 5 11 6 5 3 4 6 3 10 5 5 5 6 5 4 3 2 4 5 7 4 5 4 4 6 3 18 4 5 5 6 5 5 3 3 3 4 6 4 5 4 4 7 3 14 4 5 5 6 5 6 3 3 3 4 6 4 5 4 4 7 3 12 4 5 7 6 5 6 3 5 4 5 8 4 5 4 5 7 3 16 5 5 6 7 4 3 3 2 6 7 6 3 5 4 4 6 1 12 5 4 6 6 3 8 3 4 3 4 2 4 5 5 4 6 3 10 4 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 12 4 3 5 6 5 4 3 3 5 6 5 5 5 4 5 5 4 14 4 3 4 7 5 4 3 3 5 6 6 5 5 4 5 5 4 16 5 3 5 6 5 4 5 3 6 7 5 5 5 4 6 5 4 10 5 3 7 6 5 4 3 3 5 6 5 5 5 4 5 5 4 12 5 3 5 6 5 5 3 3 4 5 5 5 5 4 6 6 4 8 7 3 6 6 5 4 3 3 5 6 5 5 5 4 4 6 4 10 7 3 6 6 5 4 3 3 5 6 5 4 5 4 5 6 4 8 5 5 5 6 5 2 5 2 3 4 6 4 5 4 4 6 4 16 5 4 6 8 2 6 3 5 3 4 5 5 5 4 5 7 3 10 4 5 4 6 5 3 3 3 2 3 4 4 5 4 5 6 3 9 4 4 6 6 5 4 3 3 4 5 5 5 5 4 5 6 4 10 7 5 5 6 5 4 3 3 3 4 8 2 5 4 4 5 3 17 7 3 7 6 5 5 3 3 4 5 5 5 5 4 5 6 4 12 7 3 6 6 5 4 3 3 6 7 5 4 5 4 5 6 4 8 5

11 : Y 36 STR 1429 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 3 6 6 4 5 3 4 5 6 3 5 5 4 5 6 4 8 7 6 6 7 5 8 3 3 5 6 7 5 5 4 6 6 4 10 5 5 6 7 5 8 3 3 5 6 7 5 6 4 5 7 4 6 5 5 6 7 5 10 3 3 5 6 7 5 5 4 5 7 4 10 5 3 5 6 6 8 5 3 3 4 4 5 5 4 5 6 2 6 8 5 4 5 5 4 3 3 3 4 6 5 5 4 4 6 4 10 5 5 4 6 5 6 2 2 3 4 6 6 5 4 3 6 4 14 4 3 6 6 7 8 5 3 3 4 6 5 5 4 5 8 2 8 8 3 5 6 6 10 5 3 3 4 4 5 5 4 5 6 2 5 8 3 5 6 6 6 5 3 3 4 6 5 5 3 4 8 2 8 8 3 6 6 6 8 5 3 3 4 6 5 5 5 5 6 2 6 8 3 5 6 7 6 5 3 3 4 6 5 5 4 4 8 2 8 8 3 5 6 6 8 5 3 3 4 5 5 5 4 6 7 2 8 8 3 5 6 6 8 5 3 3 4 6 5 5 4 3 7 2 8 8 3 5 6 6 8 5 3 3 4 4 5 5 4 5 5 2 6 8 3 6 6 6 10 5 3 3 4 6 5 5 4 5 7 2 6 8 3 5 6 6 6 5 2 3 4 6 5 5 4 4 8 2 10 8 5 3 3 5 6 3 3 3 4 6 5 5 4 6 6 4 10 5 5 3 4 4 5 3 3 3 4 6 5 5 4 4 6 4 14 5 5 5 5 5 5 3 3 3 4 4 5 5 4 4 6 4 12 5 3 5 6 6 8 5 3 3 4 6 5 5 4 4 7 2 8 8 3 5 6 6 10 5 3 3 4 6 4 5 4 6 6 2 8 8 3 5 6 5 6 5 4 3 4 6 5 5 4 5 6 2 6 9 5 4 5 5 4 3 2 3 4 6 5 5 4 4 6 4 14 5 5 4 5 5 4 3 3 3 4 6 5 5 4 4 6 4 12 5 5 6 6 5 3 3 3 2 3 5 5 5 4 5 6 4 12 5 5 7 4 5 4 3 1 4 5 5 5 4 4 4 6 3 5 7 3 5 6 5 4 3 3 4 5 5 5 5 4 5 5 5 10 5 3 7 6 5 4 3 3 5 6 2 5 5 3 5 5 4 10 5 3 7 6 5 4 3 3 5 6 2 5 5 5 5 5 4 12 5 5 4 5 4 4 3 3 2 3 6 5 5 4 4 6 4 12 5 4 6 6 3 5 3 4 3 4 4 4 5 5 5 7 3 6 4 4 7 8 3 5 3 4 3 4 4 4 5 5 5 6 3 8 4 5 5 5 5 5 3 3 3 4 6 5 5 4 6 6 4 14 5 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 5 6 6 5 6 3 3 4 5 3 5 5 4 4 6 4 14 7 4 6 6 3 6 3 4 3 4 5 4 5 5 5 7 3 14 2 6 4 8 3 6 3 4 3 4 4 4 5 5 5 6 3 8 4

1430 HEREDITAS (Beijing) 2012 34 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 4 5 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 3 4 7 6 3 6 3 4 3 4 4 4 5 5 5 6 3 8 5 4 7 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 4 6 5 3 6 3 4 3 4 4 4 5 5 5 7 3 10 4 5 7 6 5 6 3 3 3 4 5 5 5 4 4 6 4 8 7 5 3 3 5 6 3 3 3 4 4 5 5 4 4 6 4 12 5 5 5 6 5 6 3 3 5 6 5 5 5 4 4 6 4 14 8 4 5 4 5 6 3 3 4 5 7 5 5 5 4 6 4 8 7 4 5 8 3 8 3 4 3 4 4 4 5 5 5 5 3 8 4 4 5 6 3 8 3 3 3 4 4 4 5 5 5 6 3 10 4 4 6 8 3 8 3 4 3 4 4 4 6 5 5 6 3 8 4 4 5 8 3 8 3 4 3 4 4 4 6 5 6 6 3 8 4 5 6 6 5 8 3 3 4 5 3 5 5 4 4 6 4 17 7 5 6 6 5 8 3 3 5 6 7 5 5 4 4 6 4 16 5 5 5 5 5 8 2 3 3 4 6 4 5 4 5 6 4 10 5 3 7 6 5 8 5 3 3 4 6 5 5 2 6 6 2 6 8 3 6 6 6 10 5 3 4 5 7 5 5 4 5 7 2 8 8 3 5 6 6 8 5 3 3 4 7 5 5 4 5 8 2 6 8 5 5 6 5 3 3 2 4 5 5 4 5 4 4 6 3 12 5 5 7 6 4 5 3 3 4 5 8 3 5 4 5 7 3 12 8 3 5 5 5 4 3 3 4 5 5 5 5 4 5 5 4 15 5 5 5 5 6 4 3 3 3 4 6 5 5 4 3 5 4 12 5 5 7 6 5 5 3 1 5 6 5 5 4 4 4 5 3 5 8 5 4 6 5 5 3 4 5 6 10 4 5 4 4 7 4 8 7 3 5 6 5 5 3 3 5 6 5 5 5 4 5 6 4 14 5 4 6 6 3 6 3 4 3 4 4 4 4 5 5 6 3 12 4 5 5 5 4 6 3 3 3 4 6 5 5 4 5 5 4 12 5 5 3 4 5 6 3 3 3 4 6 5 5 4 4 7 4 12 5 5 4 5 5 6 2 3 3 4 6 4 5 4 5 6 4 10 5 3 5 6 6 6 5 3 3 4 6 5 5 4 6 7 2 10 8 4 5 8 1 6 3 4 3 4 4 5 5 4 5 7 3 8 5 4 6 6 3 6 3 4 3 4 4 4 5 5 5 7 3 10 4 4 5 6 3 6 3 4 3 4 4 4 5 5 5 6 3 8 2 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 4 5 6 3 6 3 4 3 4 4 4 5 5 5 5 3 10 4 4 6 6 3 6 3 4 3 4 4 4 5 5 7 6 3 10 4 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 8 4

11 : Y 36 STR 1431 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 4 6 6 3 6 3 4 4 5 4 4 5 5 5 6 3 10 5 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 8 4 5 6 6 5 6 3 3 4 5 6 5 5 4 4 6 4 14 7 5 6 6 5 6 3 4 3 4 5 5 5 4 4 6 4 10 7 5 7 6 4 6 3 3 5 6 5 5 5 4 3 6 4 14 7 5 6 5 5 6 3 3 4 5 5 5 5 4 5 6 4 16 7 5 6 6 5 6 3 3 4 5 5 4 5 4 4 6 4 12 7 5 6 6 5 6 3 3 4 5 5 5 5 4 5 6 4 14 7 4 6 6 3 8 3 4 3 4 4 4 5 5 5 6 3 8 4 4 6 6 3 8 3 4 3 4 4 4 5 5 5 6 3 10 4 5 4 6 5 8 3 3 4 5 6 5 5 4 5 5 4 12 7 3 5 6 6 8 5 3 4 5 5 5 5 4 5 7 2 6 8 3 4 8 6 11 5 3 5 6 6 5 5 4 5 6 2 12 7 5 7 6 5 8 3 3 2 3 5 5 5 4 4 6 4 17 7 4 5 6 3 6 3 4 3 4 4 4 5 4 5 6 3 10 4 4 6 6 3 8 3 4 3 4 4 4 5 5 5 6 3 8 4 5 4 8 3 6 3 4 3 4 4 4 5 5 5 6 3 8 4 4 5 8 1 6 3 4 3 4 4 5 5 4 5 7 3 8 4 4 6 6 3 8 3 4 3 4 5 4 5 5 5 6 3 8 4 4 6 6 3 6 3 5 3 4 4 4 5 5 5 5 3 6 3 4 7 6 3 5 3 4 4 5 4 4 5 5 5 6 3 10 4 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 6 4 4 6 6 3 6 3 4 3 4 4 3 5 5 5 6 3 10 4 4 6 6 5 5 3 3 4 5 5 5 5 4 4 6 4 17 7 6 5 6 5 8 3 3 5 6 6 5 5 4 5 6 3 5 7 5 6 6 5 5 3 3 3 4 5 6 5 4 4 6 4 12 7 5 5 6 5 6 3 4 4 5 5 5 4 4 4 6 4 18 7 5 5 6 5 6 3 3 3 4 5 5 5 4 4 6 4 12 7 4 7 8 3 8 3 4 3 4 4 3 5 5 5 7 3 8 5 5 6 6 5 5 3 3 5 6 5 5 5 4 4 6 4 14 7 5 6 6 5 6 3 3 4 5 5 5 5 4 4 6 4 12 8 5 5 6 3 8 5 3 3 4 6 4 5 4 6 6 4 14 7 6 6 6 5 8 3 4 5 6 12 6 5 4 6 6 4 12 7 3 5 6 5 4 3 3 5 6 5 5 5 4 5 5 4 12 5 2 4 4 5 2 3 3 4 5 6 4 6 3 7 6 3 12 3 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 4 6 6 3 5 3 4 3 4 4 4 5 5 5 7 3 8 4 5 6 6 5 6 3 3 3 4 5 5 5 4 4 6 4 12 7 5 5 6 5 5 3 4 4 5 9 8 5 4 4 6 3 10 8 4 5 4 5 5 2 3 4 5 5 4 5 4 6 6 3 12 5 3 7 6 6 6 5 3 3 4 4 5 5 4 5 6 2 6 8 3 5 6 5 3 3 3 6 7 5 5 5 5 5 5 4 10 5

1432 HEREDITAS (Beijing) 2012 34 3 S99 S13 S32 S105 S11 S109 S110 DYS602 DYS390 S112 S23 S115 S117 S6 S10 S120 S4 DYS448a19 4 6 6 3 6 3 4 3 4 4 4 5 5 5 6 3 10 4 5 4 4 5 8 3 3 3 4 6 5 5 4 4 7 4 12 5 5 4 5 5 5 3 3 4 5 6 5 5 4 5 6 4 12 7 7 6 6 4 6 3 3 4 5 4 6 3 4 4 6 4 5 4 5 4 4 5 8 3 3 4 5 6 5 5 4 6 6 4 12 5 5 5 6 5 10 3 3 4 5 6 5 5 4 6 6 4 8 7 附表 4 16 个姜氏样本 41 个 Y-STR 单倍型信息 S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 S17 S18 S19 S20 S21 135 1 7 6 4 4 5 5 7 6 4 3 3 5 2 1 3 6 2 4 294 7 6 6 4 4 4 5 4 5 6 4 3 5 6 4 1 3 7 4 4 5 498 3 5 6 2 4 4 5 5 4 9 4 3 4 6 4 1 3 8 4 4 5 608 3 7 7 2 4 6 3 6 4 7 4 6 3 6 4 1 2 5 6 3 4 626 8 5 6 4 4 4 5 4 5 5 4 3 5 6 4 1 4 7 4 4 5 662 7 9 6 4 4 5 8 3 6 4 3 6 6 4 1 2 7 5 4 5 741 4 4 6 4 4 5 5 4 5 4 4 3 7 6 2 1 3 5 6 4 5 P003 5 3 6 2 4 4 7 6 4 7 4 3 6 6 4 1 3 8 6 3 5 P008 4 6 6 2 4 4 6 6 4 7 5 3 5 6 4 1 3 11 7 3 5 P019 4 7 6 2 4 4 6 7 4 7 5 3 5 6 4 1 3 10 6 3 5 P037 4 7 6 2 4 4 6 7 4 7 5 3 5 6 4 1 3 10 7 3 5 P047 7 9 6 2 4 4 6 6 4 7 5 3 5 6 4 1 3 9 5 3 5 P053 4 7 6 2 4 4 6 6 4 7 5 3 5 6 4 1 3 11 7 3 5 P058 5 7 6 4 4 6 5 6 7 4 4 3 7 6 2 1 3 6 5 4 5 P044 4 7 6 2 4 4 6 6 4 7 5 3 5 6 4 1 3 11 6 3 5 P069 4 7 6 2 4 4 6 6 4 7 5 3 5 6 4 1 3 10 7 3 5 S22 S23 S24 S25 S26 S27 S28 S29 S30 S31 S32 S33 S34 S35 S36 S37 S38 S39 S40 S41 S42 135 3 5 7 4 5 6 5 3 3 6 7 5 5 5 4 6 5 4 8 5 294 3 7 4 5 1 6 6 4 3 3 4 6 6 5 4 5 6 4 8 4 498 3 5 5 7 6 5 5 3 3 4 5 3 5 5 4 4 6 4 14 7 608 2 5 6 5 8 6 5 4 3 2 5 6 10 6 5 3 4 6 3 10 5 626 3 6 5 5 5 5 5 8 3 3 4 5 6 5 4 4 5 6 4 12 7 662 3 6 6 5 5 6 5 10 3 3 5 6 8 7 4 4 4 6 3 10 8 741 3 5 6 5 5 6 5 4 3 3 5 6 5 5 5 4 6 5 8 5 P003 3 5 4 4 5 8 3 8 3 4 3 4 4 4 5 5 6 6 3 8 4 P008 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 7 3 10 4 P019 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 7 3 10 4 P037 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 6 3 10 4 P047 3 5 4 4 6 8 2 8 3 4 3 4 6 5 5 5 5 6 3 8 4 P053 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 6 3 10 4 P058 3 5 6 3 5 6 5 4 3 3 5 6 3 5 5 4 5 5 4 12 4 P044 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 7 3 10 4 P069 3 5 4 4 6 8 2 6 3 4 3 4 4 5 5 4 5 7 3 10 4