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1 Journal of Ningxia Medical University EEFSEC 8q24 张玉荣 1,2, 王建业 3, 史晓红 2, 刘铭 2, 杨泽 2 1, 霍正浩 EEFSEC A 8q24 C T prostate cancer Pca SNP Pca 1 EEFSEC A 8q24 C T P > EEFSEC A 8q24 C T 3 SNPs 3 8q24 AA Pca P = SNPs Pca Gleason PSA P > EEFSEC A 8q24 C T 3 SNPs Pca 8q24 AA Pca EEFSEC 8q24 R A prostate cancer Pca T 3 SNPs Pca 4 Pca 3 SNPs Pca 1 SNPs Pca 50 1 Pca Pca 1. 1 Pca ± 6 Pca 3 Pca ± 8 PSA < 4 ng ml - 1 Pca Pca 39 EEFSEC A 8q24 C BJ E - mail huozhh@163. com 1. 2 DNA Bio Chain Taq DNA dntp LC - green PLUS Idaho 1. 3 PCR MJ PTC PCR HRM

2 / Idaho Lightscanner TMHR - I96 DNA Ep- DNA DNA DNA pendorf Biophotometer / 1. 5 Bio - Rad Gel DOC - 5 Oligo 6. 0 primer Beckma Al- 1 ~ 2 legra TM 21R C DNA EDTA 0. 5mL DNA SNPs PCR / /bp TTGGCCTGTGTGTTACA GGCATACGCTTTGTG AGGAGAATTGCAAACT CCTTGATATGCAATTAGC CAAGCAGTGATCCGTTT GCAAGTAAGTGCTAAAATAAAGGTA TTAAATTATAAAATATTTATAATATTAATTATATATATATAAATAT AATA 60 TATTATATTTATATATATATAATTAATATTATAAATATTTTATAAT TTAA GCGCGGCCGGCACTGACCCGAGACTCTGAGCGGCTGCTGGAGGTGCGGAAGCGGAGGGGCGGG 90 CCCGCCCCTCCGCTTCCGCACCTCCAGCAGCCGCTCAGAGTCTCGGGTCAGTGCCGGCCGCGC 2 3 SNPs 5-3 / /bp AGCCAGAGGATGGTAAAT GGCATACGCTTTGTG AAACGGTTGTGTACTCAC CCTTGATATGCAATTAGC TAAGTGGAGCATAATATTGTTCTAC GCAAGTAAGTGCTAAAATAAAGGTA 1. 6 HRM MDR - SPSS 5 DNA OR PCR 95% 95% CI Pca P < '- AGCCAGAGGATGGTAAAT - 3' 5'- GGCATACGCTTTGTG - 3' PCR 50μL DNA 5μL 10 PCR Buffer 5μL 10 mmol L - 1 dntp 1μL Taq DNA 5U μl - 1 1μL 10pmol q24 T 8q24 T 3 CC CT TT HRM μl μL 50μL PCR 95 5 min s s s min PCR HRM HRM 1. 7 Hardy - Weinberg 100% 2 3 SHEsis BLAST

10. EEFSEC 8q24 983 2. 2 EEFSEC A 8q24 EEFSEC A 8q24 C T T 3 Pca Pca Hardy - Weinberg P > P > 0. 05 0. 05 3 3 3 SNPs Pca SNPs n = 212 n = 231 P OR 95% CI A 177 0. 417 192 0. 416 0. 955 1. 008 0.

3 10. EEFSEC 8q EEFSEC A 8q24 EEFSEC A 8q24 C T T 3 Pca Pca Hardy - Weinberg P > P > SNPs Pca SNPs n = 212 n = 231 P OR 95% CI A ~ C AA CC AC HEW P A ~ C AA CC AC HEW P C ~ T CC TT CT HEW P SNPs MDR EEFSEC A 8q SNPs C T 3 SNPs 3 SNPs P = / MDR EEFSEC 8q24 3 SNPs 3 EEFSEC A 8q24 C T

4 EEFSEC A 8q C T 8q24 C AA Pca P P = SNPs Pca Gleason /10 PSA /10 SNP /10 5 P > SNPs Pca PSA AA AC + CC P AA AC + CC P CC TC + TT P / < Gleason < PSA / ng ml < T < SNPs 3 SNP SNP Pca SNPs

5 10. EEFSEC 8q SNPs 1 Jamal A Siegel R Ward E et al Cancer statistics J. CA Cancer J Clin Jamal A Kulldorff M Devesa SS et al. A geographic Pca analysis of prostate cancer mortality in the United States J. Int J Cancer EEFSEC A 8q24 3 Schaid DJ. The complex genetic epidemiology of prostate cancer J. Hum Mol Genet Spec No 1 C T 3 Pca R P > Ryo T Shusuke A Michiaki K et al. Genome - wide SNPs association study identifies five new susceptibility loci 3 SNPs for prostate cancer in the Japanese population J. Nature genetics Pca 5. 3 J q q24 8q24 8q24 J Pca q24 rsl q24 14 SNP 17 Chris- J tiane R 8q24 SNPs Pca 18 EEFSEC 8. 8q24 SNP EEFSEC Pca J Chiara G and Matthew LF. Chromosome 8q24 Associated Cancers and MYC J. Genes & Cancer Crowther - Swanepoel D Broderick P Di Bernardo MC et al. Common variants at 2q q Pca 15q21. 3 and 16q24. 1 influence chronic lymphocytic leukemia risk J. Nat Genet Pca 11 Ghoussaini M Song H Koessler T et al. Multiple loci 8q24 C with different cancer specificities within the 8q24 gene desert J. J Natl Cancer Inst AA Pca 12 Eeles RA Kote - Jarai Z Giles GG et al. Multiple P = Pca Gleason PSA newly identified loci associated with prostate cancer susceptibility J. Nat Genet P > Easton DF Pooley KA Dunning AM et al. Genome - Pca wide association study identifies novel breast cancer - - susceptibility loci J. Nature EEFSEC A 8q24 C T 14 Turnbull C Ahmed S Morrison J et al. Genome - Pca 8q24 wide association study identifies five new breast cancer C AA Pca susceptibility loci J. Nat Genet P = Pca ( 下转第 1008 页 )

6 difference of serum LHBs in different HBV - DNA level groups F = had statistically significant P < Serum LHBs showed direct correlation with HBV - DNA level r = P < The LHBs positive patients' ALT AST markers were higher than those in the HBV - LP negative patients. Conclusion LH- Bs showed a high coincidence rate with HBV - DNA. Monitoring serum LHBs is a good adding test for diagnosing HBV replication and active. Key words hepatitis B virus hepatitis B virus large surface protein hepatitis B virus DNA ( 上接第 985 页 ) 15 Thomas G Jacobs KB Yeager M et al. Multiple loci identified in a genome - wide association study of prostate cancer J. Nat Genet Eeles RA Kote - Jarai Z Al Olama AA et al. Identification of seven new prostate cancer susceptibility loci through a genome - wide association study J. Nat Genet Ahmadiyeh N Pomerantz MM Grisanzio C et al. 8q24 prostate breast and colon cancer risk loci show tissue - specific long - range interaction with MYC J. Proc Natl Acad Sci U S A Christiana R Jada BT Stanley H et al. Confirmation study of prostate cancer risk variants at 8q24 in African Americans identifies a novel risk locus J. Cold Spring Harbor Laboratory Press The Association between EEFSEC 8q24 and Prostate Cancer in Chinese Han Populations ZHANG Yu - rong 1 2 WANG Jian - ye 3 SHI Xiao - hong 2 LIU Ming 2 YANG Ze 2 HUO Zheng - hao 1 1. Basic Medical Academy of Ningxia Med. Univ. Yinchuan Dept. of Beijing Hospital Key State Laboratory of Ministry of Health Beijing Urology of Beijing Hospital Beijing Abstract Objective To explore the associations between 3 SNPs at EEFSEC and at 8q24 and Pca and the relative clinical risk factors in Chinese Han populations. Methods Blood samples and personal information of 212 Pca patients and 231 controls were collected. The frequencies of alleles and genotypes of these 3 SNPs in two groups were analyzed based on the model of case - control and case - only studies. We also explored the association between these loci and the clinical characteristics. Results 1 There is no significantly different distribution of risk alleles and genotype frequencies in cases and controls among the association of EEFSEC A and 8q24 C T and Pca P > The interaction of 3 SNPs in EEFSEC A and 8q24 C T showed that EEFSEC and 8q24 had synergism. 3 The interaction between genes and the environment people with genotype AA on the site of had less probability to get Pca when getting older P = Other sites had no significant relevance P < with the age of Pca on - set PSA level Gleason and pathology on indicators. Conclusion Combined EEFSEC A and 8q24 C T might raise the risk of Pca of Chinese Han people. People with genotype AA on the site of have less probability to get Pca when getting older. Key words gene EEFSEC 8q24 prostate cancer

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<4D6963726F736F667420576F7264202D20B2CCA3BA4542B2A1B6BE52746120496747BFB9CCE5D3EBB1C7D1CAB0A9B7D6C6DAB5C4B9D8CFB52E646F63> EB 病毒 Rta/IgG 抗体与鼻咽癌分期的关系蔡永林, 郑裕明, 成积儒, 李军, 莫永坤, 钟青燕 ( 广西梧州市红十字会医院, 梧州市肿瘤防治研究所, 广西梧州 543002) 摘要 : 目的探讨 EB 病毒 Rta/IgG 抗体与鼻咽癌临床分期的关系方法用酶